Rw5G026680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0019932
Physical Location & Seq
Forward (+)
38673956 .. 38674727
772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026680.1

Sequence Viewer

Length: 693 bp
ATGAGCAGAAATTCAACCAAACTTCCCATTCAATTCCCTTCCATCAACAACTTCGTCACCATCGTCTCCTCCGTCGTCGTTTTCCATAACATCCATGACTCCTGCCTCTCCCACCTCCACGACCTCATTTGGGACATCATTCCCTCCCCCAATTCCCTCCATTACCACAAATCCTGGGCTTTGGATGCTTCTTCCTCCTCTGTCTTCCCCGCGCCTTCTACAAGCTCGGCCGATTCGATGCCACCGCTGGTGGCGGTCTCACTTCTCACTCTGGTTCTGGATTCGAAGCAGATGGACAAGGTTTGGAGTGACCTATTCAAAAGGCACAATGATTACCGGATTATGGTTGATGCGCCTTATGCTCTCAATTTGACCACTCTGGTTGTGAGTTTCAACCGAAATCAAAATTACCTAGATTTCTTGGTCATGGGTTCTGGTCTGCGGAACATGCTTCATTTAACTAATGCATCCGATTATGGTGCTTCATTGCAATTCACTAAGGATGATGCTGGTTCTTTGGTCTCATCTGAGCAGCATGAAGAAGCAATAATTACAGGGTCCGTGCTGGTCAGACCTTCGTCGCATCTCTTTTGGGTTGGGATACCCACTTTGATCAACTTGATGCTAAATACAGATGAGAAGAGGGAGAAGATAACTGATGTCTTGAGAGCTGCCTATGATGAGCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

25.72

Weight (kDa)

5.82

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019932)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0042551
rosa_multiflora Rmu_co8010648.1_g000001
rosa_rugosa Rorug05G0201500 Rorug05G0201900
rosa_samantha Rh5BG292000 Rh5DG301800
rosa_wichuraiana Rw5G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 212
AciI CCGC 4 cut(s) 210, 245, 254, 442
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 10
AfiI CCNNNNNNNGG 2 cut(s) 130, 343
AgsI TTSAA 4 cut(s) 15, 32, 319, 394
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 2 cut(s) 225, 671
AluI AGCT 2 cut(s) 225, 671
Alw26I GTCTC 3 cut(s) 70, 262, 526
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 532, 671
ApoI RAATTY 1 cut(s) 10
AspLEI GCGC 2 cut(s) 214, 355
AspS9I GGNCC 1 cut(s) 558
AsuHPI GGTGA 1 cut(s) 49
AsuII TTCGAA 1 cut(s) 284
AvaII GGWCC 1 cut(s) 558
BbsI GAAGAC 1 cut(s) 196
BbvI GCAGC 2 cut(s) 544, 658
BccI CCATC 3 cut(s) 50, 68, 286
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 1 cut(s) 175
BciVI GTATCC 1 cut(s) 594
BclI TGATCA 1 cut(s) 612
BcoDI GTCTC 3 cut(s) 70, 262, 526
BfaI CTAG 1 cut(s) 413
BfuI GTATCC 1 cut(s) 594
BisI GCNGC 2 cut(s) 533, 672
BlsI GCNGC 2 cut(s) 534, 673
Bme1390I CCNGG 1 cut(s) 175
Bme18I GGWCC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 1 cut(s) 175
BmsI GCATC 7 cut(s) 175, 228, 340, 476, 496, 592, 612
BoxI GACNNNNGTC 1 cut(s) 577
BpiI GAAGAC 1 cut(s) 196
Bpu14I TTCGAA 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 685
BsaBI GATNNNNATC 1 cut(s) 684
BsaI GGTCTC 2 cut(s) 262, 526
BsaJI CCNNGG 1 cut(s) 174
BsaWI WCCGGW 1 cut(s) 336
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 343
Bse3DI GCAATG 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 684
BseBI CCWGG 1 cut(s) 175
BseDI CCNNGG 1 cut(s) 174
BseGI GGATG 4 cut(s) 90, 190, 467, 508
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 2 cut(s) 130, 343
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 519
BseRI GAGGAG 2 cut(s) 58, 187
BseX3I CGGCCG 1 cut(s) 228
BseXI GCAGC 2 cut(s) 544, 658
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 1 cut(s) 230
BsiEI CGRYCG 1 cut(s) 231
BsiSI CCGG 1 cut(s) 337
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 2 cut(s) 130, 343
BsmAI GTCTC 3 cut(s) 70, 262, 526
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 2 cut(s) 262, 526
Bsp119I TTCGAA 1 cut(s) 284
Bsp143I GATC 1 cut(s) 612
BspACI CCGC 4 cut(s) 210, 245, 254, 442
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 559
BspT104I TTCGAA 1 cut(s) 284
BspTNI GGTCTC 2 cut(s) 262, 526
BsrDI GCAATG 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 175
Bst6I CTCTTC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 284
BstDEI CTNAG 2 cut(s) 498, 528
BstF5I GGATG 4 cut(s) 90, 190, 467, 508
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 2 cut(s) 214, 355
BstKTI GATC 1 cut(s) 615
BstMAI GTCTC 3 cut(s) 70, 262, 526
BstMBI GATC 1 cut(s) 612
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 3 cut(s) 185, 359, 448
BstNI CCWGG 1 cut(s) 175
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 577
BstSCI CCNGG 1 cut(s) 173
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 544, 658
BstV2I GAAGAC 1 cut(s) 196
BstZI CGGCCG 1 cut(s) 228
BsuI GTATCC 1 cut(s) 594
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 4 cut(s) 90, 190, 467, 508
CfoI GCGC 2 cut(s) 214, 355
Cfr13I GGNCC 1 cut(s) 558
CspCI CAANNNNNGTGG 2 cut(s) 364, 399
CviAII CATG 4 cut(s) 95, 427, 448, 536
CviJI RGCY 4 cut(s) 179, 225, 230, 671
CviKI_1 RGCY 4 cut(s) 179, 225, 230, 671
DdeI CTNAG 2 cut(s) 498, 528
DpnI GATC 1 cut(s) 614
DpnII GATC 1 cut(s) 612
EaeI YGGCCR 1 cut(s) 228
EagI CGGCCG 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 635
EarI CTCTTC 1 cut(s) 635
EclXI CGGCCG 1 cut(s) 228
Eco31I GGTCTC 2 cut(s) 262, 526
Eco47I GGWCC 1 cut(s) 558
Eco52I CGGCCG 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 173
EcoT22I ATGCAT 1 cut(s) 469
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 4 cut(s) 98, 430, 451, 539
FaiI YATR 9 cut(s) 87, 96, 344, 360, 428, 449, 477, 537, 678
FaqI GGGAC 1 cut(s) 146
FatI CATG 4 cut(s) 94, 426, 447, 535
FauI CCCGC 1 cut(s) 217
FbaI TGATCA 1 cut(s) 612
Fnu4HI GCNGC 2 cut(s) 533, 672
FokI GGATG 4 cut(s) 77, 197, 454, 515
Fsp4HI GCNGC 2 cut(s) 533, 672
FspBI CTAG 1 cut(s) 413
GlaI GCGC 2 cut(s) 213, 354
GluI GCNGC 2 cut(s) 533, 672
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 337
HhaI GCGC 2 cut(s) 214, 355
Hin1II CATG 4 cut(s) 98, 430, 451, 539
Hin6I GCGC 2 cut(s) 212, 353
HinP1I GCGC 2 cut(s) 212, 353
HinfI GANTC 3 cut(s) 98, 233, 281
HpaII CCGG 1 cut(s) 337
HphI GGTGA 1 cut(s) 49
Hpy188I TCNGA 3 cut(s) 472, 529, 572
Hpy188III TCNNGA 2 cut(s) 278, 664
Hpy99I CGWCG 3 cut(s) 77, 80, 583
HpyAV CCTTC 3 cut(s) 48, 225, 585
HpyCH4V TGCA 2 cut(s) 467, 490
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 359, 448
HpyF3I CTNAG 2 cut(s) 498, 528
Hsp92II CATG 4 cut(s) 98, 430, 451, 539
HspAI GCGC 2 cut(s) 212, 353
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 1 cut(s) 612
Lsp1109I GCAGC 2 cut(s) 544, 658
LweI GCATC 7 cut(s) 175, 228, 340, 476, 496, 592, 612
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 2 cut(s) 55, 308
MalI GATC 1 cut(s) 614
MboI GATC 1 cut(s) 612
MboII GAAGA 5 cut(s) 183, 196, 551, 652, 661
MluCI AATT 7 cut(s) 10, 32, 151, 367, 406, 491, 549
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 9 cut(s) 79, 116, 125, 134, 154, 167, 205, 208, 636
Mph1103I ATGCAT 1 cut(s) 469
MseI TTAA 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 247
MspI CCGG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 3 cut(s) 185, 359, 448
NdeII GATC 1 cut(s) 612
NlaIII CATG 4 cut(s) 98, 430, 451, 539
NlaIV GGNNCC 1 cut(s) 559
NmeAIII GCCGAG 1 cut(s) 206
NmuCI GTSAC 2 cut(s) 55, 308
NsiI ATGCAT 1 cut(s) 469
NspI RCATGY 1 cut(s) 451
NspV TTCGAA 1 cut(s) 284
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 69, 233
PfeI GAWTC 2 cut(s) 233, 281
PkrI GCNGC 2 cut(s) 534, 673
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PshAI GACNNNNGTC 1 cut(s) 577
Psp6I CCWGG 1 cut(s) 173
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 1 cut(s) 558
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 2 cut(s) 533, 672
Sau3AI GATC 1 cut(s) 612
Sau96I GGNCC 1 cut(s) 558
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 175
SetI ASST 8 cut(s) 117, 126, 227, 303, 315, 414, 577, 673
SfaNI GCATC 7 cut(s) 175, 228, 340, 476, 496, 592, 612
SfuI TTCGAA 1 cut(s) 284
SinI GGWCC 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 664
SmoI CTYRAG 1 cut(s) 664
Sse9I AATT 7 cut(s) 10, 32, 151, 367, 406, 491, 549
SsiI CCGC 4 cut(s) 210, 245, 254, 442
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 173
TaqI TCGA 2 cut(s) 236, 284
TasI AATT 7 cut(s) 10, 32, 151, 367, 406, 491, 549
TfiI GAWTC 2 cut(s) 233, 281
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TseFI GTSAC 2 cut(s) 55, 308
TseI GCWGC 2 cut(s) 532, 671
Tsp45I GTSAC 2 cut(s) 55, 308
TspDTI ATGAA 3 cut(s) 443, 474, 552
TspGWI ACGGA 2 cut(s) 61, 550
VpaK11BI GGWCC 1 cut(s) 558
XapI RAATTY 1 cut(s) 10
XceI RCATGY 1 cut(s) 451
XspI CTAG 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.