Rw7G013510

50S ribosomal protein L22, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0020265
Physical Location & Seq
Forward (+)
13027084 .. 13027763
680 bp
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UTR
Exon/CDS
Intron
Rw7G013510.1

Sequence Viewer

Length: 420 bp
ATGGAGAAAAAGACCAGAAAGCCGAACCAATATACAGAAGTAGCCGCTTCAGGCCAACATATATGTATTTCTGCTCACAAAGCGAGAAGAGTAATTGATCAAATTCGTGGGCGTTCCTATGAAGAAACACTTATGATACTAGAACTCATGCCTTATCGAGCATGTTATGACATTTTAAAATTGGTTTATTCTGCAGCAGCAAACGCTAATCACAATAAAGGTTTGAATGAAACAAGTTTAATCGTTAGTAAAGCTGAAGTCAACGAGGGCACCACTGTGAAAAAATTAAAACCCCGAGCTCGAGGGCGGGGTTATCCGATAAGAAGATCCACCTGTCATATAACTATTGTGTTGAAAGATATATCTGTAGATGAACAACTAGAAAAAGATAAAAGGACAAAAGAGCGGAGGAATATTTAA

Protein Analysis

139

Amino Acids

15.94

Weight (kDa)

9.85

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22 PF00237 16 - 119 2.9e-33 Ribosomal protein L22p/L17e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020265)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00810
rosa_chinensis RchiOBHm_Chr1g0329561 RchiOBHm_Chr5g0025841 RchiOBHm_Chr6g0286361 RchiOBHm_CPg0501841
rosa_wichuraiana Rw7G013510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccBSI CCGCTC 1 cut(s) 406
AciI CCGC 3 cut(s) 45, 307, 406
AclWI GGATC 1 cut(s) 321
AcsI RAATTY 1 cut(s) 102
AcuI CTGAAG 2 cut(s) 33, 276
AgsI TTSAA 2 cut(s) 226, 355
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 2 cut(s) 254, 299
AluI AGCT 2 cut(s) 254, 299
Alw21I GWGCWC 1 cut(s) 301
AlwI GGATC 1 cut(s) 321
Ama87I CYCGRG 2 cut(s) 294, 300
AoxI GGCC 1 cut(s) 52
ApeKI GCWGC 2 cut(s) 194, 197
ApoI RAATTY 1 cut(s) 102
AvaI CYCGRG 2 cut(s) 294, 300
BaeGI GKGCMC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 301
BbvI GCAGC 2 cut(s) 206, 209
BclI TGATCA 1 cut(s) 97
BfaI CTAG 2 cut(s) 140, 380
BfmI CTRYAG 2 cut(s) 192, 366
BisI GCNGC 3 cut(s) 45, 195, 198
BlsI GCNGC 3 cut(s) 46, 196, 199
BmeT110I CYCGRG 2 cut(s) 294, 300
BmiI GGNNCC 1 cut(s) 271
BseSI GKGCMC 1 cut(s) 272
BseXI GCAGC 2 cut(s) 206, 209
BshFI GGCC 1 cut(s) 54
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 301
BsiHKCI CYCGRG 2 cut(s) 294, 300
BsnI GGCC 1 cut(s) 54
BsoBI CYCGRG 2 cut(s) 294, 300
Bsp1286I GDGCHC 2 cut(s) 272, 301
Bsp143I GATC 2 cut(s) 97, 326
BspACI CCGC 3 cut(s) 45, 307, 406
BspANI GGCC 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 321
BspT107I GGYRCC 1 cut(s) 269
BsrBI CCGCTC 1 cut(s) 406
BssMI GATC 2 cut(s) 97, 326
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 82
BstKTI GATC 2 cut(s) 100, 329
BstMBI GATC 2 cut(s) 97, 326
BstMWI GCNNNNNNNGC 2 cut(s) 80, 203
BstNSI RCATGY 1 cut(s) 165
BstSFI CTRYAG 2 cut(s) 192, 366
BstSLI GKGCMC 1 cut(s) 272
BstV1I GCAGC 2 cut(s) 206, 209
BstX2I RGATCY 1 cut(s) 326
BstYI RGATCY 1 cut(s) 326
BsuRI GGCC 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 273
CviAII CATG 2 cut(s) 148, 162
CviJI RGCY 5 cut(s) 22, 44, 54, 254, 299
CviKI_1 RGCY 5 cut(s) 22, 44, 54, 254, 299
DpnI GATC 2 cut(s) 99, 328
DpnII GATC 2 cut(s) 97, 326
DraI TTTAAA 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 299
Eco24I GRGCYC 1 cut(s) 301
Eco53kI GAGCTC 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 33, 276
Eco88I CYCGRG 2 cut(s) 294, 300
EcoICRI GAGCTC 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 301
FaeI CATG 2 cut(s) 151, 165
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 2 cut(s) 147, 161
FauI CCCGC 1 cut(s) 300
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 3 cut(s) 45, 195, 198
FriOI GRGCYC 1 cut(s) 301
Fsp4HI GCNGC 3 cut(s) 45, 195, 198
FspBI CTAG 2 cut(s) 140, 380
GluI GCNGC 3 cut(s) 45, 195, 198
HaeIII GGCC 1 cut(s) 54
Hin1II CATG 2 cut(s) 151, 165
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 1 cut(s) 318
Hpy8I GTNNAC 1 cut(s) 262
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 203
Hsp92II CATG 2 cut(s) 151, 165
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 2 cut(s) 97, 326
LpnPI CCDG 3 cut(s) 28, 36, 346
Lsp1109I GCAGC 2 cut(s) 206, 209
MaeI CTAG 2 cut(s) 140, 380
MalI GATC 2 cut(s) 99, 328
MbiI CCGCTC 1 cut(s) 406
MboI GATC 2 cut(s) 97, 326
MboII GAAGA 3 cut(s) 99, 134, 336
MflI RGATCY 1 cut(s) 326
MhlI GDGCHC 2 cut(s) 272, 301
MluCI AATT 4 cut(s) 93, 102, 179, 284
MnlI CCTC 3 cut(s) 259, 296, 402
MseI TTAA 4 cut(s) 176, 239, 287, 418
MslI CAYNNNNRTG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 80, 203
NdeII GATC 2 cut(s) 97, 326
NlaIII CATG 2 cut(s) 151, 165
NlaIV GGNNCC 1 cut(s) 271
NspI RCATGY 1 cut(s) 165
OliI CACNNNNGTG 1 cut(s) 275
PaeR7I CTCGAG 1 cut(s) 300
PkrI GCNGC 3 cut(s) 46, 196, 199
Psp124BI GAGCTC 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 271
PspXI VCTCGAGB 1 cut(s) 300
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 326
RseI CAYNNNNRTG 1 cut(s) 275
SacI GAGCTC 1 cut(s) 301
SaqAI TTAA 4 cut(s) 176, 239, 287, 418
SatI GCNGC 3 cut(s) 45, 195, 198
Sau3AI GATC 2 cut(s) 97, 326
SduI GDGCHC 2 cut(s) 272, 301
SetI ASST 4 cut(s) 223, 256, 301, 335
SfcI CTRYAG 2 cut(s) 192, 366
Sfr274I CTCGAG 1 cut(s) 300
SlaI CTCGAG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
Sse9I AATT 4 cut(s) 93, 102, 179, 284
SsiI CCGC 3 cut(s) 45, 307, 406
SspI AATATT 1 cut(s) 415
SspMI CTAG 2 cut(s) 140, 380
SstI GAGCTC 1 cut(s) 301
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 2 cut(s) 157, 301
TasI AATT 4 cut(s) 93, 102, 179, 284
TauI GCSGC 1 cut(s) 47
Tru1I TTAA 4 cut(s) 176, 239, 287, 418
Tru9I TTAA 4 cut(s) 176, 239, 287, 418
TscAI CASTG 1 cut(s) 280
TseI GCWGC 2 cut(s) 194, 197
TspDTI ATGAA 3 cut(s) 135, 243, 387
TspRI CASTG 1 cut(s) 280
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 165
XhoI CTCGAG 1 cut(s) 300
XspI CTAG 2 cut(s) 140, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.