Rw7G020650

Galactose oxidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0018208
Physical Location & Seq
Reverse (-)
23636288 .. 23637728
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020650.1

Sequence Viewer

Length: 1281 bp
ATGACAACTAAATACTTTCTGCTGTCGTTCTCCCTCTTGATCACCGCAGCTGCTGCTACTTCCGGCGGCGGCGGCCGGTGGCAGGTCTTGCAGAAAAGTATTGGGATTTCAGCCATGCACATGCAGCTGCTGCGCAACGACCGAGTCATCATGTTCGACCGAACGGATTTCGGAAAATCAAATTTGTCGCTGCCGAGGGGAATCTGCCGGAGGAACCCAAACGACACCGCGTTGAAGGTCGACTGTACCGCACATTCGGTCGAGTACGACGTGGCAAGCAACTCTTTCCGACCGCTCATGCTGTTTTCCGACACTTGGTGCTCCTCCGGGTCCGTCAGCCCCAACGGCAGTCTCGTTCAGACTGGGGGGTGGAACGACGGTGAGCTAAGGGTGAGACTGTTTGAGCCATGCAGCAGTTGTGATTGGGAGGAGATTGCGCATGCGCTGGCCCACCCAAGGTGGTATTCCACAAATCACCTGCTGCCAGATAGCAGGCTTATAATCGTTGGCGGACGCTCGCAGCCAAACTATGAATTTTTTCCCAAGACCAAGGACGGGGAGGGGTCCATCACTGTGCCTTTCCTTGACGAAACAAAGGACGCCGTAGAGGACAATCTGTACCCTTTTGTTTTTCTCAACGTTGATGGCAATTTATTCATATATGCCAACAATCGAGCAATTCTTCTGAACTACGTTACCGGAGAAACTGTGAAGACTTATCCCACAATATCGGGCGGCGATCCGAGATCGTACCCCAGCACCGGTTCCGCCATCATGAATCCGACGTCCATCCCTGGCATGTACCACTCGACCGCCGTTCTGTTACGTGACGGTCGCGTGTTGGTCGGTGGGAGCAATCCACACGAGGGATACGTGTTTTCCTCCGTGCTCTTCCCAACCGAACTTTCCTTGGAAGCATTTTATCCGGAATATCTCAATCCTGAATTCTCCGATGTTCGTCCCACAGTGAGTGCACCCAAATCTCAACAAGTTGTTGGCTACGGGAAAAAGGTACAGATTCAATTTTCTATATCCGGGAAACTTGCCGCTCAATCCTTGTTCGCATCGATGATTTCTCCATCATTTTCAACCCACTCGTTCTCCATGAGCCAACGGTCATTACTTCTCTCTATTGACAATGTCGTATCAGCGGGTAATCGGACATATCAAGGGCAGCTCACCATGCCAAGTGATCCTAACCTGGCCCCCTCTTCGTATTTTATGCTCTATGTTGTTCATCAAAACATACCAAGTGAGGCCACTTGGATCCGCATTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1929 Glyoxal_oxid_N

Protein Analysis

426

Amino Acids

46.93

Weight (kDa)

6.31

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxal_oxid_N PF07250 39 - 261 3.8e-100 Glyoxal oxidase N-terminus
Glyoxal_oxid_N PF07250 258 - 311 2e-20 Glyoxal oxidase N-terminus
GO-like_E_set PF09118 320 - 425 3.7e-25 Galactose oxidase-like, Early set domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018208)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0196161
rosa_laevigata RLG00000003166 RLG00000004057
rosa_multiflora Rmu_sc0000821.1_g000022 Rmu_sc0033762.1_g000001
rosa_roxburghii Rroxscaffold_3G00249710 Rroxscaffold_3G00259710
rosa_rugosa Rorug07G0031400
rosa_samantha Rh7CG162600
rosa_wichuraiana Rw7G013430 Rw7G020650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AarI CACCTGC 1 cut(s) 486
AatII GACGTC 1 cut(s) 788
Acc16I TGCGCA 2 cut(s) 134, 438
Acc36I ACCTGC 2 cut(s) 73, 486
AccBSI CCGCTC 2 cut(s) 295, 1049
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 230, 837
AccIII TCCGGA 1 cut(s) 925
AclI AACGTT 1 cut(s) 639
AclWI GGATC 4 cut(s) 734, 1187, 1261, 1274
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 3 cut(s) 181, 533, 944
AcyI GRCGYC 2 cut(s) 600, 785
AdeI CACNNNGTG 1 cut(s) 318
AfaI GTAC 6 cut(s) 247, 266, 620, 752, 803, 1014
AfiI CCNNNNNNNGG 6 cut(s) 82, 315, 456, 555, 761, 866
AflIII ACRYGT 1 cut(s) 873
AgeI ACCGGT 1 cut(s) 761
AgsI TTSAA 3 cut(s) 235, 1022, 1089
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 2 cut(s) 793, 1200
AjuI GAANNNNNNNTTGG 4 cut(s) 448, 480, 889, 921
AluBI AGCT 4 cut(s) 50, 127, 385, 1177
AluI AGCT 4 cut(s) 50, 127, 385, 1177
Alw21I GWGCWC 3 cut(s) 323, 891, 976
Alw26I GTCTC 2 cut(s) 356, 388
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 4 cut(s) 734, 1187, 1261, 1274
AlwNI CAGNNNCTG 2 cut(s) 53, 130
Aor13HI TCCGGA 1 cut(s) 925
AoxI GGCC 4 cut(s) 73, 447, 1203, 1257
ApaLI GTGCAC 1 cut(s) 972
ApoI RAATTY 3 cut(s) 181, 533, 944
AsiGI ACCGGT 1 cut(s) 761
Asp700I GAANNNNTTC 1 cut(s) 537
AspLEI GCGC 3 cut(s) 135, 439, 445
AspS9I GGNCC 4 cut(s) 330, 448, 564, 1204
AsuC2I CCSGG 2 cut(s) 328, 1036
AsuHPI GGTGA 5 cut(s) 34, 392, 403, 467, 1171
AvaII GGWCC 2 cut(s) 330, 564
BaeGI GKGCMC 1 cut(s) 976
BamHI GGATCC 1 cut(s) 1266
BauI CACGAG 1 cut(s) 863
BbsI GAAGAC 1 cut(s) 719
Bbv12I GWGCWC 3 cut(s) 323, 891, 976
BccI CCATC 5 cut(s) 575, 638, 779, 797, 1087
BceAI ACGGC 3 cut(s) 361, 587, 800
BciT130I CCWGG 2 cut(s) 795, 1202
BciVI GTATCC 1 cut(s) 863
BclI TGATCA 1 cut(s) 39
BcnI CCSGG 2 cut(s) 328, 1036
BcoDI GTCTC 2 cut(s) 356, 388
BfuAI ACCTGC 2 cut(s) 73, 486
BfuI GTATCC 1 cut(s) 863
BglI GCCNNNNNGGC 1 cut(s) 345
Bme1390I CCNGG 4 cut(s) 328, 795, 1036, 1202
Bme18I GGWCC 2 cut(s) 330, 564
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 4 cut(s) 330, 448, 564, 1204
BmiI GGNNCC 6 cut(s) 215, 331, 565, 766, 1206, 1268
BmrFI CCNGG 4 cut(s) 328, 795, 1036, 1202
BmrI ACTGGG 1 cut(s) 372
BmsI GCATC 1 cut(s) 1073
BmuI ACTGGG 1 cut(s) 372
BpiI GAAGAC 1 cut(s) 719
Bpu10I CCTNAGC 1 cut(s) 386
BpuMI CCSGG 2 cut(s) 328, 1036
Bsa29I ATCGAT 1 cut(s) 1067
BsaAI YACGTR 2 cut(s) 827, 874
BsaHI GRCGYC 2 cut(s) 600, 785
BsaJI CCNNGG 5 cut(s) 194, 455, 549, 793, 909
BsaWI WCCGGW 3 cut(s) 698, 761, 925
BsaXI ACNNNNNCTCC 4 cut(s) 693, 723, 1085, 1115
Bsc4I CCNNNNNNNGG 6 cut(s) 82, 315, 456, 555, 761, 866
Bse118I RCCGGY 2 cut(s) 75, 761
Bse1I ACTGG 1 cut(s) 367
Bse3DI GCAATG 1 cut(s) 1272
BseAI TCCGGA 1 cut(s) 925
BseBI CCWGG 2 cut(s) 795, 1202
BseCI ATCGAT 1 cut(s) 1067
BseDI CCNNGG 5 cut(s) 194, 455, 549, 793, 909
BseGI GGATG 1 cut(s) 789
BseLI CCNNNNNNNGG 6 cut(s) 82, 315, 456, 555, 761, 866
BseMI GCAATG 1 cut(s) 1272
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 2 cut(s) 313, 443
BseSI GKGCMC 1 cut(s) 976
BseX3I CGGCCG 1 cut(s) 73
BseYI CCCAGC 1 cut(s) 755
Bsh1236I CGCG 2 cut(s) 230, 837
Bsh1285I CGRYCG 7 cut(s) 76, 142, 160, 261, 293, 813, 835
BshFI GGCC 4 cut(s) 75, 449, 1205, 1259
BshTI ACCGGT 1 cut(s) 761
BshVI ATCGAT 1 cut(s) 1067
BsiEI CGRYCG 7 cut(s) 76, 142, 160, 261, 293, 813, 835
BsiHKAI GWGCWC 3 cut(s) 323, 891, 976
BsiSI CCGG 8 cut(s) 63, 76, 208, 327, 699, 762, 926, 1035
BslFI GGGAC 1 cut(s) 945
BslI CCNNNNNNNGG 6 cut(s) 82, 315, 456, 555, 761, 866
BsmAI GTCTC 2 cut(s) 356, 388
BsmFI GGGAC 1 cut(s) 945
BsnI GGCC 4 cut(s) 75, 449, 1205, 1259
Bsp1286I GDGCHC 3 cut(s) 323, 891, 976
Bsp13I TCCGGA 1 cut(s) 925
Bsp143I GATC 5 cut(s) 39, 739, 746, 1192, 1266
BspANI GGCC 4 cut(s) 75, 449, 1205, 1259
BspDI ATCGAT 1 cut(s) 1067
BspEI TCCGGA 1 cut(s) 925
BspFNI CGCG 2 cut(s) 230, 837
BspHI TCATGA 1 cut(s) 774
BspLI GGNNCC 6 cut(s) 215, 331, 565, 766, 1206, 1268
BspMI ACCTGC 2 cut(s) 73, 486
BspPI GGATC 4 cut(s) 734, 1187, 1261, 1274
BspQI GCTCTTC 1 cut(s) 896
BsrBI CCGCTC 2 cut(s) 295, 1049
BsrDI GCAATG 1 cut(s) 1272
BsrFI RCCGGY 2 cut(s) 75, 761
BsrI ACTGG 1 cut(s) 367
BssAI RCCGGY 2 cut(s) 75, 761
BssECI CCNNGG 5 cut(s) 194, 455, 549, 793, 909
BssMI GATC 5 cut(s) 39, 739, 746, 1192, 1266
BssNI GRCGYC 2 cut(s) 600, 785
BssSI CACGAG 1 cut(s) 863
BssT1I CCWWGG 3 cut(s) 455, 549, 909
Bst2BI CACGAG 1 cut(s) 863
Bst2UI CCWGG 2 cut(s) 795, 1202
Bst4CI ACNGT 8 cut(s) 245, 380, 399, 574, 709, 833, 967, 1116
Bst6I CTCTTC 2 cut(s) 896, 1216
BstACI GRCGYC 2 cut(s) 600, 785
BstAPI GCANNNNNTGC 3 cut(s) 53, 88, 130
BstBAI YACGTR 2 cut(s) 827, 874
BstC8I GCNNGC 5 cut(s) 277, 441, 447, 494, 518
BstDEI CTNAG 1 cut(s) 386
BstF5I GGATG 1 cut(s) 789
BstFNI CGCG 2 cut(s) 230, 837
BstHHI GCGC 3 cut(s) 135, 439, 445
BstKTI GATC 5 cut(s) 42, 742, 749, 1195, 1269
BstMAI GTCTC 2 cut(s) 356, 388
BstMBI GATC 5 cut(s) 39, 739, 746, 1192, 1266
BstMCI CGRYCG 7 cut(s) 76, 142, 160, 261, 293, 813, 835
BstMWI GCNNNNNNNGC 7 cut(s) 53, 72, 88, 124, 130, 345, 1183
BstNI CCWGG 2 cut(s) 795, 1202
BstNSI RCATGY 3 cut(s) 124, 443, 802
BstSCI CCNGG 4 cut(s) 326, 793, 1034, 1200
BstSLI GKGCMC 1 cut(s) 976
BstUI CGCG 2 cut(s) 230, 837
BstV2I GAAGAC 1 cut(s) 719
BstX2I RGATCY 1 cut(s) 1266
BstYI RGATCY 1 cut(s) 1266
BstZI CGGCCG 1 cut(s) 73
Bsu15I ATCGAT 1 cut(s) 1067
BsuI GTATCC 1 cut(s) 863
BsuRI GGCC 4 cut(s) 75, 449, 1205, 1259
BsuTUI ATCGAT 1 cut(s) 1067
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 570, 972
BveI ACCTGC 2 cut(s) 73, 486
Cac8I GCNNGC 5 cut(s) 277, 441, 447, 494, 518
CaiI CAGNNNCTG 2 cut(s) 53, 130
CciI TCATGA 1 cut(s) 774
CfoI GCGC 3 cut(s) 135, 439, 445
Cfr10I RCCGGY 2 cut(s) 75, 761
Cfr13I GGNCC 4 cut(s) 330, 448, 564, 1204
ClaI ATCGAT 1 cut(s) 1067
CseI GACGC 2 cut(s) 522, 608
Csp6I GTAC 6 cut(s) 246, 265, 619, 751, 802, 1013
CspAI ACCGGT 1 cut(s) 761
CviQI GTAC 6 cut(s) 246, 265, 619, 751, 802, 1013
DdeI CTNAG 1 cut(s) 386
DpnI GATC 5 cut(s) 41, 741, 748, 1194, 1268
DpnII GATC 5 cut(s) 39, 739, 746, 1192, 1266
DraIII CACNNNGTG 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 73
EagI CGGCCG 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 896, 1216
EarI CTCTTC 2 cut(s) 896, 1216
EciI GGCGGA 2 cut(s) 525, 757
EclXI CGGCCG 1 cut(s) 73
Eco130I CCWWGG 3 cut(s) 455, 549, 909
Eco47I GGWCC 2 cut(s) 330, 564
Eco52I CGGCCG 1 cut(s) 73
EcoRI GAATTC 1 cut(s) 944
EcoRII CCWGG 2 cut(s) 793, 1200
EcoT14I CCWWGG 3 cut(s) 455, 549, 909
ErhI CCWWGG 3 cut(s) 455, 549, 909
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FaqI GGGAC 1 cut(s) 945
FauI CCCGC 1 cut(s) 1144
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 240
FokI GGATG 1 cut(s) 776
FspI TGCGCA 2 cut(s) 134, 438
GlaI GCGC 3 cut(s) 134, 438, 444
GsaI CCCAGC 1 cut(s) 759
HaeIII GGCC 4 cut(s) 75, 449, 1205, 1259
HapII CCGG 8 cut(s) 63, 76, 208, 327, 699, 762, 926, 1035
HgaI GACGC 2 cut(s) 522, 608
HhaI GCGC 3 cut(s) 135, 439, 445
Hin1I GRCGYC 2 cut(s) 600, 785
Hin6I GCGC 3 cut(s) 133, 437, 443
HinP1I GCGC 3 cut(s) 133, 437, 443
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 4 cut(s) 144, 201, 778, 1018
HpaII CCGG 8 cut(s) 63, 76, 208, 327, 699, 762, 926, 1035
HphI GGTGA 5 cut(s) 34, 392, 403, 467, 1171
Hpy166II GTNNAC 2 cut(s) 241, 974
Hpy188I TCNGA 9 cut(s) 173, 290, 310, 360, 687, 744, 783, 952, 1161
Hpy188III TCNNGA 4 cut(s) 37, 775, 926, 941
Hpy8I GTNNAC 2 cut(s) 241, 974
Hpy99I CGWCG 3 cut(s) 272, 380, 787
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 8 cut(s) 245, 380, 399, 574, 709, 833, 967, 1116
HpyCH4IV ACGT 6 cut(s) 270, 639, 693, 785, 826, 873
HpyCH4V TGCA 5 cut(s) 91, 118, 124, 411, 974
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 72, 88, 124, 130, 345, 1183
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 6 cut(s) 270, 639, 693, 785, 826, 873
Hsp92I GRCGYC 2 cut(s) 600, 785
HspAI GCGC 3 cut(s) 133, 437, 443
Kpn2I TCCGGA 1 cut(s) 925
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 5 cut(s) 39, 739, 746, 1192, 1266
LguI GCTCTTC 1 cut(s) 896
LmnI GCTCC 2 cut(s) 326, 852
LweI GCATC 1 cut(s) 1073
MaeII ACGT 6 cut(s) 270, 639, 693, 785, 826, 873
MaeIII GTNAC 3 cut(s) 694, 822, 827
MalI GATC 5 cut(s) 41, 741, 748, 1194, 1268
MbiI CCGCTC 2 cut(s) 295, 1049
MboI GATC 5 cut(s) 39, 739, 746, 1192, 1266
MboII GAAGA 4 cut(s) 674, 724, 883, 1203
MflI RGATCY 1 cut(s) 1266
MhlI GDGCHC 3 cut(s) 323, 891, 976
MluCI AATT 6 cut(s) 181, 533, 649, 678, 944, 1022
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 3 cut(s) 313, 333, 806
MroI TCCGGA 1 cut(s) 925
MroXI GAANNNNTTC 1 cut(s) 537
MslI CAYNNNNRTG 2 cut(s) 119, 572
MspA1I CMGCKG 3 cut(s) 50, 127, 1151
MspI CCGG 8 cut(s) 63, 76, 208, 327, 699, 762, 926, 1035
MspR9I CCNGG 4 cut(s) 328, 795, 1036, 1202
MvaI CCWGG 2 cut(s) 795, 1202
MvnI CGCG 2 cut(s) 230, 837
MwoI GCNNNNNNNGC 7 cut(s) 53, 72, 88, 124, 130, 345, 1183
NciI CCSGG 2 cut(s) 328, 1036
NdeII GATC 5 cut(s) 39, 739, 746, 1192, 1266
NlaIV GGNNCC 6 cut(s) 215, 331, 565, 766, 1206, 1268
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 827
NsbI TGCGCA 2 cut(s) 134, 438
NspI RCATGY 3 cut(s) 124, 443, 802
PaeI GCATGC 1 cut(s) 443
PagI TCATGA 1 cut(s) 774
PaqCI CACCTGC 1 cut(s) 486
PciSI GCTCTTC 1 cut(s) 896
PcsI WCGNNNNNNNCGW 3 cut(s) 267, 351, 870
PdmI GAANNNNTTC 1 cut(s) 537
PfeI GAWTC 3 cut(s) 201, 778, 1018
PflFI GACNNNGTC 2 cut(s) 143, 1139
PfoI TCCNGGA 1 cut(s) 1034
PinAI ACCGGT 1 cut(s) 761
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Ppu21I YACGTR 2 cut(s) 827, 874
PsiI TTATAA 1 cut(s) 500
Psp1406I AACGTT 1 cut(s) 639
Psp6I CCWGG 2 cut(s) 793, 1200
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 2 cut(s) 793, 1200
PspN4I GGNNCC 6 cut(s) 215, 331, 565, 766, 1206, 1268
PspPI GGNCC 4 cut(s) 330, 448, 564, 1204
PstNI CAGNNNCTG 2 cut(s) 53, 130
PsuI RGATCY 1 cut(s) 1266
PsyI GACNNNGTC 2 cut(s) 143, 1139
PvuII CAGCTG 2 cut(s) 50, 127
RsaI GTAC 6 cut(s) 247, 266, 620, 752, 803, 1014
RsaNI GTAC 6 cut(s) 246, 265, 619, 751, 802, 1013
RseI CAYNNNNRTG 2 cut(s) 119, 572
SalI GTCGAC 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 896
Sau3AI GATC 5 cut(s) 39, 739, 746, 1192, 1266
Sau96I GGNCC 4 cut(s) 330, 448, 564, 1204
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 4 cut(s) 328, 795, 1036, 1202
SduI GDGCHC 3 cut(s) 323, 891, 976
SfaNI GCATC 1 cut(s) 1073
SinI GGWCC 2 cut(s) 330, 564
SmiMI CAYNNNNRTG 2 cut(s) 119, 572
SphI GCATGC 1 cut(s) 443
Sse9I AATT 6 cut(s) 181, 533, 649, 678, 944, 1022
StyD4I CCNGG 4 cut(s) 326, 793, 1034, 1200
StyI CCWWGG 3 cut(s) 455, 549, 909
TaaI ACNGT 8 cut(s) 245, 380, 399, 574, 709, 833, 967, 1116
TaiI ACGT 6 cut(s) 273, 642, 696, 788, 829, 876
TaqI TCGA 6 cut(s) 156, 240, 261, 673, 809, 1067
TaqII GACCGA 3 cut(s) 156, 174, 247
TasI AATT 6 cut(s) 181, 533, 649, 678, 944, 1022
TauI GCSGC 5 cut(s) 69, 72, 75, 738, 1049
TfiI GAWTC 3 cut(s) 201, 778, 1018
TscAI CASTG 2 cut(s) 577, 972
TseFI GTSAC 1 cut(s) 827
Tsp45I GTSAC 1 cut(s) 827
TspDTI ATGAA 4 cut(s) 546, 646, 791, 1226
TspGWI ACGGA 3 cut(s) 179, 322, 874
TspRI CASTG 2 cut(s) 577, 972
Tth111I GACNNNGTC 2 cut(s) 143, 1139
VneI GTGCAC 1 cut(s) 972
VpaK11BI GGWCC 2 cut(s) 330, 564
XapI RAATTY 3 cut(s) 181, 533, 944
XceI RCATGY 3 cut(s) 124, 443, 802
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 537
ZraI GACGTC 1 cut(s) 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.