AT1G01695

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004678
Physical Location & Seq
Forward (+)
252626 .. 254530
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01695.1

Sequence Viewer

Length: 1302 bp
ATGCCAGTCGAAAAACATGTGGAAGAAAAGCTTCGCTCAAATAGAAAAGAGAGAGGCTGGATATCTGGTATTTTCAGAAGGAAGAAGAGATCACCACAAGAAGAAGAAGTTGATAATGAAAATAACTCATCTGAAGATTCTCGTTTGATGGGAGCCAAGAATTTGCATTTGTTTCTCTCTGAGATCATGAGAAAACTGAAACATGCAATCAGAAAGGAGAAACCGGACAAGAGATTGTTAGGGAAGAAGAAAAGCTTTGAGAAGAGTTTGTCAACTAAAGATCATTTCTTTCTCGAGCGCATGACTTCAATATCTCAAAAGAGATTTCATCAAGAACATAATGATCCAAACTTGGCAACAAGCAAACAGGTTCAAAGAAATCACGAAAGGATTGTATGGCTTCCCGAGTATTCGTCTCCTTTTAGCAGCCCGGGAAGAATATGGAAACAGAATTCAACCGTGCTTTTGAGGTCTTCTTCATATGATTTCATCAAGTCCGAAACAATTGCAGATGATTCAATAACTTTGAAAGAGATAGGAACTGCTTCATCAAGTGAAGGTAGTAGTAGCCCTCTTCTATCTAAAAGCAATCAGATTGTTGATGAGATGTCAAAAGTTGCAGCTTATGGTGCAGAGAATGAAGGTGAATTCTTGGAAGAGACACTGTCTCTCCCCTCTGTGGGCTCACCATCTCACTATATATCTAAAACTGAGGAATATGACTTTGTTCTTGAACCACTATTCATAAGTCCAGCAAGAGGTGAAGCAAAGATACAACCTTTGTGTAACCAATTGCAAGAAAAGAATCGTCACAAAGAATCAGTGTTTAAGTATGTAAAAGCGGTGTTAGAGGCAATAGACTCAAACTGGGAAGAGCTGTATCTGAAAACAGAAATCTCTGACCAGCTTCTATATCCGGCATTGATCAGCAACATACCATTCTATCCAAATCAGCTTTGTGTTGAACATGAGCTCCTCTTCGACTGCATCAATGAAGTTCTCTTCGAATTCTGCAGGTTTCCTCAATGGGTTTCCTTTGCTGAAACCAGGACCCAAGTCTTGCCATATAGTGTTGAAAGCATTGTTCCCGAGGTTCAAGAAAAGGTTTATTGTCATCTCTTGCCAATGCAACTCCGTCGATCGTTGGAAGAAAGAGTTAGAGAAGATATGGCTAAACACAGGAGCTGGTTAGACATTAGATGTGACCTTGAGTGTATTGGCTTTGAGACTAGCGAATTGATATTGAATGAATTATTGGAACAGCTGATGCTTGAGCTTGAAGATAATCACAAAAATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

433

Amino Acids

50.4

Weight (kDa)

5.75

Isoelectric Point (pI)

60.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 276 - 420 6.8e-23 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1005
AciI CCGC 1 cut(s) 842
AclWI GGATC 1 cut(s) 338
AcsI RAATTY 4 cut(s) 160, 451, 647, 1007
AcuI CTGAAG 1 cut(s) 153
AfiI CCNNNNNNNGG 3 cut(s) 679, 680, 758
AflIII ACRYGT 1 cut(s) 16
AjnI CCWGG 1 cut(s) 1046
AjuI GAANNNNNNNTTGG 2 cut(s) 1238, 1270
AloI GAACNNNNNNTCC 2 cut(s) 957, 989
Alw21I GWGCWC 1 cut(s) 975
Alw26I GTCTC 4 cut(s) 420, 653, 672, 1220
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 1185
Ama87I CYCGRG 4 cut(s) 293, 404, 430, 1088
ApeKI GCWGC 2 cut(s) 426, 620
ApoI RAATTY 4 cut(s) 160, 451, 647, 1007
Asp700I GAANNNNTTC 2 cut(s) 30, 544
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 1050
AsuC2I CCSGG 2 cut(s) 431, 432
AsuHPI GGTGA 4 cut(s) 84, 656, 678, 773
AsuII TTCGAA 1 cut(s) 1005
AvaI CYCGRG 4 cut(s) 293, 404, 430, 1088
AvaII GGWCC 1 cut(s) 1050
BanII GRGCYC 2 cut(s) 686, 975
BarI GAAGNNNNNNTAC 2 cut(s) 756, 788
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 975
BbvI GCAGC 2 cut(s) 438, 632
BccI CCATC 2 cut(s) 142, 697
BcgI CGANNNNNNTGC 4 cut(s) 488, 522, 1118, 1152
BciT130I CCWGG 1 cut(s) 1048
BclI TGATCA 1 cut(s) 924
BcnI CCSGG 2 cut(s) 431, 432
BcoDI GTCTC 4 cut(s) 420, 653, 672, 1220
BfaI CTAG 1 cut(s) 1230
BfmI CTRYAG 1 cut(s) 1012
BfuAI ACCTGC 1 cut(s) 1005
BisI GCNGC 2 cut(s) 427, 621
BlsI GCNGC 2 cut(s) 428, 622
Bme1390I CCNGG 3 cut(s) 431, 432, 1048
Bme18I GGWCC 1 cut(s) 1050
BmeT110I CYCGRG 4 cut(s) 293, 404, 430, 1088
BmgT120I GGNCC 1 cut(s) 1050
BmiI GGNNCC 2 cut(s) 154, 1052
BmrFI CCNGG 3 cut(s) 431, 432, 1048
BmrI ACTGGG 1 cut(s) 877
BmsI GCATC 2 cut(s) 996, 1257
BmuI ACTGGG 1 cut(s) 877
BoxI GACNNNNGTC 1 cut(s) 1055
BpiI GAAGAC 1 cut(s) 465
Bpu14I TTCGAA 1 cut(s) 1005
BpuEI CTTGAG 2 cut(s) 1229, 1292
BpuMI CCSGG 2 cut(s) 431, 432
BsaJI CCNNGG 2 cut(s) 430, 1089
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 4 cut(s) 654, 684, 957, 987
Bsc4I CCNNNNNNNGG 3 cut(s) 679, 680, 758
Bse1I ACTGG 2 cut(s) 5, 872
BseBI CCWGG 1 cut(s) 1048
BseDI CCNNGG 2 cut(s) 430, 1089
BseLI CCNNNNNNNGG 3 cut(s) 679, 680, 758
BseMII CTCAG 2 cut(s) 171, 702
BseNI ACTGG 2 cut(s) 5, 872
BseRI GAGGAG 1 cut(s) 965
BseXI GCAGC 2 cut(s) 438, 632
BsgI GTGCAG 1 cut(s) 651
Bsh1285I CGRYCG 1 cut(s) 1142
BsiEI CGRYCG 1 cut(s) 1142
BsiHKAI GWGCWC 1 cut(s) 975
BsiHKCI CYCGRG 4 cut(s) 293, 404, 430, 1088
BsiSI CCGG 3 cut(s) 224, 431, 917
BslI CCNNNNNNNGG 3 cut(s) 679, 680, 758
BsmAI GTCTC 4 cut(s) 420, 653, 672, 1220
BsmBI CGTCTC 1 cut(s) 420
BsoBI CYCGRG 4 cut(s) 293, 404, 430, 1088
Bsp119I TTCGAA 1 cut(s) 1005
Bsp1286I GDGCHC 2 cut(s) 686, 975
Bsp143I GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
BspACI CCGC 1 cut(s) 842
BspCNI CTCAG 2 cut(s) 172, 703
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 2 cut(s) 154, 1052
BspMAI CTGCAG 1 cut(s) 1016
BspMI ACCTGC 1 cut(s) 1005
BspPI GGATC 1 cut(s) 338
BspQI GCTCTTC 1 cut(s) 867
BspT104I TTCGAA 1 cut(s) 1005
BsrI ACTGG 2 cut(s) 5, 872
BssECI CCNNGG 2 cut(s) 430, 1089
BssMI GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
Bst2UI CCWGG 1 cut(s) 1048
Bst4CI ACNGT 2 cut(s) 460, 666
Bst6I CTCTTC 7 cut(s) 80, 257, 579, 651, 867, 983, 1007
BstBI TTCGAA 1 cut(s) 1005
BstDEI CTNAG 2 cut(s) 180, 711
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 6 cut(s) 92, 186, 283, 346, 927, 1142
BstMAI GTCTC 4 cut(s) 420, 653, 672, 1220
BstMBI GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
BstMCI CGRYCG 1 cut(s) 1142
BstMWI GCNNNNNNNGC 1 cut(s) 629
BstNI CCWGG 1 cut(s) 1048
BstNSI RCATGY 2 cut(s) 20, 206
BstPAI GACNNNNGTC 1 cut(s) 1055
BstSCI CCNGG 3 cut(s) 429, 430, 1046
BstSFI CTRYAG 1 cut(s) 1012
BstV1I GCAGC 2 cut(s) 438, 632
BstV2I GAAGAC 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 662, 828
BveI ACCTGC 1 cut(s) 1005
CaiI CAGNNNCTG 1 cut(s) 1185
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 1050
Cfr9I CCCGGG 1 cut(s) 430
CviAII CATG 5 cut(s) 17, 187, 203, 301, 968
DdeI CTNAG 2 cut(s) 180, 711
DpnI GATC 6 cut(s) 91, 185, 282, 345, 926, 1141
DpnII GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
Eam1104I CTCTTC 7 cut(s) 80, 257, 579, 651, 867, 983, 1007
EarI CTCTTC 7 cut(s) 80, 257, 579, 651, 867, 983, 1007
Ecl136II GAGCTC 1 cut(s) 973
Eco24I GRGCYC 2 cut(s) 686, 975
Eco32I GATATC 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 1050
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 4 cut(s) 293, 404, 430, 1088
EcoICRI GAGCTC 1 cut(s) 973
EcoO109I RGGNCCY 1 cut(s) 1050
EcoRI GAATTC 3 cut(s) 451, 647, 1007
EcoRII CCWGG 1 cut(s) 1046
EcoRV GATATC 1 cut(s) 63
EcoT38I GRGCYC 2 cut(s) 686, 975
Esp3I CGTCTC 1 cut(s) 420
FaeI CATG 5 cut(s) 20, 190, 206, 304, 971
FalI AAGNNNNNCTT 4 cut(s) 15, 47, 239, 271
FatI CATG 5 cut(s) 16, 186, 202, 300, 967
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 924
Fnu4HI GCNGC 2 cut(s) 427, 621
FriOI GRGCYC 2 cut(s) 686, 975
Fsp4HI GCNGC 2 cut(s) 427, 621
FspBI CTAG 1 cut(s) 1230
GlaI GCGC 1 cut(s) 299
GluI GCNGC 2 cut(s) 427, 621
HapII CCGG 3 cut(s) 224, 431, 917
HhaI GCGC 1 cut(s) 300
Hin1II CATG 5 cut(s) 20, 190, 206, 304, 971
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HindIII AAGCTT 2 cut(s) 29, 253
HinfI GANTC 5 cut(s) 137, 515, 805, 818, 860
HpaII CCGG 3 cut(s) 224, 431, 917
HphI GGTGA 4 cut(s) 84, 656, 678, 773
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 8 cut(s) 77, 133, 181, 212, 499, 594, 885, 901
Hpy188III TCNNGA 8 cut(s) 187, 293, 332, 383, 404, 731, 1088, 1097
Hpy8I GTNNAC 1 cut(s) 273
Hpy99I CGWCG 1 cut(s) 1140
HpyAV CCTTC 3 cut(s) 72, 551, 635
HpyCH4III ACNGT 2 cut(s) 460, 666
HpyCH4V TGCA 9 cut(s) 166, 206, 509, 620, 632, 796, 987, 1014, 1129
HpyF10VI GCNNNNNNNGC 1 cut(s) 629
HpyF3I CTNAG 2 cut(s) 180, 711
Hsp92II CATG 5 cut(s) 20, 190, 206, 304, 971
HspAI GCGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 924
Kzo9I GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
LguI GCTCTTC 1 cut(s) 867
LmnI GCTCC 3 cut(s) 152, 978, 1182
Lsp1109I GCAGC 2 cut(s) 438, 632
LweI GCATC 2 cut(s) 996, 1257
MaeI CTAG 1 cut(s) 1230
MaeIII GTNAC 3 cut(s) 785, 809, 1202
MalI GATC 6 cut(s) 91, 185, 282, 345, 926, 1141
MboI GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
MfeI CAATTG 2 cut(s) 504, 791
MhlI GDGCHC 2 cut(s) 686, 975
MluCI AATT 8 cut(s) 160, 451, 504, 647, 791, 1007, 1235, 1250
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 1 cut(s) 1125
MroXI GAANNNNTTC 2 cut(s) 30, 544
MseI TTAA 2 cut(s) 828, 1300
MslI CAYNNNNRTG 1 cut(s) 1293
MspA1I CMGCKG 1 cut(s) 1264
MspI CCGG 3 cut(s) 224, 431, 917
MspR9I CCNGG 3 cut(s) 431, 432, 1048
MunI CAATTG 2 cut(s) 504, 791
MvaI CCWGG 1 cut(s) 1048
MwoI GCNNNNNNNGC 1 cut(s) 629
NciI CCSGG 2 cut(s) 431, 432
NdeI CATATG 1 cut(s) 481
NdeII GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
NlaIII CATG 5 cut(s) 20, 190, 206, 304, 971
NlaIV GGNNCC 2 cut(s) 154, 1052
NmuCI GTSAC 2 cut(s) 809, 1202
NspI RCATGY 2 cut(s) 20, 206
NspV TTCGAA 1 cut(s) 1005
PaeR7I CTCGAG 1 cut(s) 293
PagI TCATGA 1 cut(s) 186
PciI ACATGT 1 cut(s) 16
PciSI GCTCTTC 1 cut(s) 867
PdmI GAANNNNTTC 2 cut(s) 30, 544
PfeI GAWTC 4 cut(s) 137, 515, 805, 818
PflFI GACNNNGTC 1 cut(s) 664
PkrI GCNGC 2 cut(s) 428, 622
Ple19I CGATCG 1 cut(s) 1142
PleI GAGTC 1 cut(s) 854
PpsI GAGTC 1 cut(s) 854
PpuMI RGGWCCY 1 cut(s) 1050
PscI ACATGT 1 cut(s) 16
PshAI GACNNNNGTC 1 cut(s) 1055
Psp124BI GAGCTC 1 cut(s) 975
Psp5II RGGWCCY 1 cut(s) 1050
Psp6I CCWGG 1 cut(s) 1046
PspGI CCWGG 1 cut(s) 1046
PspN4I GGNNCC 2 cut(s) 154, 1052
PspPI GGNCC 1 cut(s) 1050
PspPPI RGGWCCY 1 cut(s) 1050
PstI CTGCAG 1 cut(s) 1016
PstNI CAGNNNCTG 1 cut(s) 1185
PsyI GACNNNGTC 1 cut(s) 664
PvuI CGATCG 1 cut(s) 1142
PvuII CAGCTG 1 cut(s) 1264
RseI CAYNNNNRTG 1 cut(s) 1293
SacI GAGCTC 1 cut(s) 975
SapI GCTCTTC 1 cut(s) 867
SaqAI TTAA 2 cut(s) 828, 1300
SatI GCNGC 2 cut(s) 427, 621
Sau3AI GATC 6 cut(s) 89, 183, 280, 343, 924, 1139
Sau96I GGNCC 1 cut(s) 1050
SchI GAGTC 1 cut(s) 854
ScrFI CCNGG 3 cut(s) 431, 432, 1048
SduI GDGCHC 2 cut(s) 686, 975
SfaNI GCATC 2 cut(s) 996, 1257
SfcI CTRYAG 1 cut(s) 1012
Sfr274I CTCGAG 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 1005
SinI GGWCC 1 cut(s) 1050
SlaI CTCGAG 1 cut(s) 293
SmaI CCCGGG 1 cut(s) 432
SmiMI CAYNNNNRTG 1 cut(s) 1293
SmlI CTYRAG 3 cut(s) 293, 1208, 1271
SmoI CTYRAG 3 cut(s) 293, 1208, 1271
Sse9I AATT 8 cut(s) 160, 451, 504, 647, 791, 1007, 1235, 1250
SsiI CCGC 1 cut(s) 842
SspMI CTAG 1 cut(s) 1230
SstI GAGCTC 1 cut(s) 975
StyD4I CCNGG 3 cut(s) 429, 430, 1046
TaaI ACNGT 2 cut(s) 460, 666
TaqI TCGA 5 cut(s) 9, 294, 981, 1005, 1138
TasI AATT 8 cut(s) 160, 451, 504, 647, 791, 1007, 1235, 1250
TfiI GAWTC 4 cut(s) 137, 515, 805, 818
Tru1I TTAA 2 cut(s) 828, 1300
Tru9I TTAA 2 cut(s) 828, 1300
TscAI CASTG 2 cut(s) 669, 828
TseFI GTSAC 2 cut(s) 809, 1202
TseI GCWGC 2 cut(s) 426, 620
Tsp45I GTSAC 2 cut(s) 809, 1202
TspDTI ATGAA 9 cut(s) 132, 317, 468, 478, 537, 654, 733, 1008, 1263
TspGWI ACGGA 1 cut(s) 1124
TspMI CCCGGG 1 cut(s) 430
TspRI CASTG 2 cut(s) 669, 828
Tth111I GACNNNGTC 1 cut(s) 664
VpaK11BI GGWCC 1 cut(s) 1050
XapI RAATTY 4 cut(s) 160, 451, 647, 1007
XceI RCATGY 2 cut(s) 20, 206
XhoI CTCGAG 1 cut(s) 293
XmaI CCCGGG 1 cut(s) 430
XmnI GAANNNNTTC 2 cut(s) 30, 544
XspI CTAG 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.