AT1G04480

60S ribosomal protein L23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0020844
Physical Location & Seq
Forward (+)
1215925 .. 1217471
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04480.1

Sequence Viewer

Length: 423 bp
ATGTCGAAGCGAGGGCGTGGAGGTACCTCTGGTAACAAATTCAGGATGTCACTGGGTCTTCCGGTGGCGGCGACGGTGAACTGTGCCGATAACACCGGTGCTAAGAATCTCTACATCATTTCCGTGAAAGGTATCAAAGGTCGTCTTAATCGATTACCTTCTGCTTGCGTCGGAGACATGGTGATGGCCACCGTCAAAAAGGGTAAGCCTGATCTCAGGAAAAAGGTTCTTCCTGCCGTCATCGTTAGGCAACGCAAACCATGGCGCCGAAAGGATGGTGTTTTCATGTACTTCGAAGATAATGCTGGAGTCATTGTCAACCCCAAGGGAGAAATGAAAGGTTCTGCAATTACTGGTCCTATTGGGAAAGAGTGTGCTGATCTTTGGCCAAGGATTGCTAGTGCTGCCAATGCCATTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14

Protein Analysis

140

Amino Acids

15.03

Weight (kDa)

10.48

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 21 - 140 1.2e-41 Ribosomal protein L14p/L23e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04480 AT2G33370 AT3G04400
pyrus_communis pycom13g19490
rosa_wichuraiana Rw7G016970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 23
AccB1I GGYRCC 2 cut(s) 23, 264
AciI CCGC 1 cut(s) 68
AcoI YGGCCR 2 cut(s) 186, 386
AcsI RAATTY 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 2 cut(s) 25, 290
AgeI ACCGGT 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 168
AoxI GGCC 2 cut(s) 186, 386
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 1 cut(s) 38
AsiGI ACCGGT 1 cut(s) 95
Asp718I GGTACC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 2 cut(s) 88, 193
AsuII TTCGAA 1 cut(s) 294
AvaII GGWCC 1 cut(s) 356
BalI TGGCCA 2 cut(s) 188, 388
BanI GGYRCC 2 cut(s) 23, 264
BbsI GAAGAC 1 cut(s) 50
BbvI GCAGC 1 cut(s) 391
BccI CCATC 2 cut(s) 178, 269
BceAI ACGGC 1 cut(s) 221
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 268
BisI GCNGC 2 cut(s) 69, 405
BlsI GCNGC 2 cut(s) 70, 406
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 2 cut(s) 25, 266
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 50
BpmI CTGGAG 1 cut(s) 327
Bpu14I TTCGAA 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 151
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 3 cut(s) 260, 324, 389
BsaWI WCCGGW 2 cut(s) 61, 95
BsaXI ACNNNNNCTCC 4 cut(s) 165, 195, 300, 330
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 2 cut(s) 57, 358
BseCI ATCGAT 1 cut(s) 151
BseDI CCNNGG 3 cut(s) 260, 324, 389
BseGI GGATG 2 cut(s) 51, 280
BseMII CTCAG 1 cut(s) 229
BseNI ACTGG 2 cut(s) 57, 358
BseXI GCAGC 1 cut(s) 391
BshFI GGCC 2 cut(s) 188, 388
BshNI GGYRCC 2 cut(s) 23, 264
BshTI ACCGGT 1 cut(s) 95
BshVI ATCGAT 1 cut(s) 151
BsiSI CCGG 2 cut(s) 62, 96
BsmAI GTCTC 1 cut(s) 168
BsnI GGCC 2 cut(s) 188, 388
Bsp119I TTCGAA 1 cut(s) 294
Bsp143I GATC 2 cut(s) 211, 379
Bsp19I CCATGG 1 cut(s) 260
BspACI CCGC 1 cut(s) 68
BspANI GGCC 2 cut(s) 188, 388
BspCNI CTCAG 1 cut(s) 228
BspDI ATCGAT 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 25, 266
BspT104I TTCGAA 1 cut(s) 294
BspT107I GGYRCC 2 cut(s) 23, 264
BsrFI RCCGGY 1 cut(s) 95
BsrI ACTGG 2 cut(s) 57, 358
BssAI RCCGGY 1 cut(s) 95
BssECI CCNNGG 3 cut(s) 260, 324, 389
BssMI GATC 2 cut(s) 211, 379
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 3 cut(s) 260, 324, 389
Bst4CI ACNGT 3 cut(s) 76, 83, 193
BstACI GRCGYC 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 2 cut(s) 102, 215
BstDSI CCRYGG 1 cut(s) 260
BstF5I GGATG 2 cut(s) 51, 280
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 2 cut(s) 214, 382
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 2 cut(s) 211, 379
BstMWI GCNNNNNNNGC 2 cut(s) 404, 410
BstV1I GCAGC 1 cut(s) 391
BstV2I GAAGAC 1 cut(s) 50
Bsu15I ATCGAT 1 cut(s) 151
BsuRI GGCC 2 cut(s) 188, 388
BsuTUI ATCGAT 1 cut(s) 151
BtgI CCRYGG 1 cut(s) 260
BtsCI GGATG 2 cut(s) 51, 280
BtsIMutI CAGTG 1 cut(s) 50
Cac8I GCNNGC 1 cut(s) 166
CfoI GCGC 1 cut(s) 267
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 356
ClaI ATCGAT 1 cut(s) 151
CseI GACGC 1 cut(s) 157
Csp6I GTAC 2 cut(s) 24, 289
CspAI ACCGGT 1 cut(s) 95
CviAII CATG 3 cut(s) 178, 261, 286
CviJI RGCY 3 cut(s) 188, 208, 388
CviKI_1 RGCY 3 cut(s) 188, 208, 388
CviQI GTAC 2 cut(s) 24, 289
DdeI CTNAG 2 cut(s) 102, 215
DinI GGCGCC 1 cut(s) 266
DpnI GATC 2 cut(s) 213, 381
DpnII GATC 2 cut(s) 211, 379
EaeI YGGCCR 2 cut(s) 186, 386
Eco130I CCWWGG 3 cut(s) 260, 324, 389
Eco47I GGWCC 1 cut(s) 356
EcoT14I CCWWGG 3 cut(s) 260, 324, 389
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
ErhI CCWWGG 3 cut(s) 260, 324, 389
FaeI CATG 3 cut(s) 181, 264, 289
FaiI YATR 3 cut(s) 179, 262, 287
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FatI CATG 3 cut(s) 177, 260, 285
Fnu4HI GCNGC 2 cut(s) 69, 405
FokI GGATG 2 cut(s) 58, 287
Fsp4HI GCNGC 2 cut(s) 69, 405
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 266
GluI GCNGC 2 cut(s) 69, 405
GsuI CTGGAG 1 cut(s) 327
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 2 cut(s) 188, 388
HapII CCGG 2 cut(s) 62, 96
HgaI GACGC 1 cut(s) 157
HhaI GCGC 1 cut(s) 267
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 3 cut(s) 181, 264, 289
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 2 cut(s) 106, 309
HpaII CCGG 2 cut(s) 62, 96
HphI GGTGA 2 cut(s) 88, 193
Hpy166II GTNNAC 2 cut(s) 79, 319
Hpy188I TCNGA 1 cut(s) 173
Hpy188III TCNNGA 2 cut(s) 43, 217
Hpy8I GTNNAC 2 cut(s) 79, 319
Hpy99I CGWCG 2 cut(s) 76, 173
HpyAV CCTTC 1 cut(s) 168
HpyCH4III ACNGT 3 cut(s) 76, 83, 193
HpyCH4V TGCA 1 cut(s) 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 410
HpyF3I CTNAG 2 cut(s) 102, 215
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 3 cut(s) 181, 264, 289
HspAI GCGC 1 cut(s) 265
KasI GGCGCC 1 cut(s) 264
KpnI GGTACC 1 cut(s) 27
Kzo9I GATC 2 cut(s) 211, 379
Lsp1109I GCAGC 1 cut(s) 391
MaeI CTAG 1 cut(s) 399
MaeIII GTNAC 2 cut(s) 32, 48
MalI GATC 2 cut(s) 213, 381
MboI GATC 2 cut(s) 211, 379
MboII GAAGA 3 cut(s) 50, 221, 308
MlsI TGGCCA 2 cut(s) 188, 388
MluCI AATT 2 cut(s) 38, 348
MluNI TGGCCA 2 cut(s) 188, 388
Mly113I GGCGCC 1 cut(s) 265
MlyI GAGTC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 151
MnlI CCTC 3 cut(s) 5, 14, 37
Mox20I TGGCCA 2 cut(s) 188, 388
MscI TGGCCA 2 cut(s) 188, 388
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 2 cut(s) 122, 182
Msp20I TGGCCA 2 cut(s) 188, 388
MspI CCGG 2 cut(s) 62, 96
MwoI GCNNNNNNNGC 2 cut(s) 404, 410
NarI GGCGCC 1 cut(s) 265
NcoI CCATGG 1 cut(s) 260
NdeII GATC 2 cut(s) 211, 379
NlaIII CATG 3 cut(s) 181, 264, 289
NlaIV GGNNCC 2 cut(s) 25, 266
NmuCI GTSAC 1 cut(s) 48
NspV TTCGAA 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 148
PfeI GAWTC 1 cut(s) 106
PinAI ACCGGT 1 cut(s) 95
PkrI GCNGC 2 cut(s) 70, 406
PleI GAGTC 1 cut(s) 317
PluTI GGCGCC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 25, 266
PspPI GGNCC 1 cut(s) 356
RsaI GTAC 2 cut(s) 25, 290
RsaNI GTAC 2 cut(s) 24, 289
RseI CAYNNNNRTG 2 cut(s) 122, 182
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 69, 405
Sau3AI GATC 2 cut(s) 211, 379
Sau96I GGNCC 1 cut(s) 356
SchI GAGTC 1 cut(s) 318
SetI ASST 7 cut(s) 25, 29, 133, 142, 160, 228, 343
SfoI GGCGCC 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 294
SgrAI CRCCGGYG 1 cut(s) 95
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 2 cut(s) 122, 182
Sse9I AATT 2 cut(s) 38, 348
SsiI CCGC 1 cut(s) 68
SspDI GGCGCC 1 cut(s) 264
SspMI CTAG 1 cut(s) 399
StyI CCWWGG 3 cut(s) 260, 324, 389
TaaI ACNGT 3 cut(s) 76, 83, 193
TaqI TCGA 3 cut(s) 5, 151, 294
TasI AATT 2 cut(s) 38, 348
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 57
TseFI GTSAC 1 cut(s) 48
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 48
TspDTI ATGAA 2 cut(s) 274, 350
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 1 cut(s) 38
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.