Rw7G016970

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0020844
Physical Location & Seq
Forward (+)
17531092 .. 17531514
423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016970.1

Sequence Viewer

Length: 423 bp
ATGTCGAAGCGAGGGCGCGGAGGGTCTGCCGGAAACAAGTTCTGGATGTCGCTGGGTCTGCCCGTGGCAGCGACGGTGAACTGCGCCGACAACACCGGTGCCAAGAATCTCTACATTATCTCTGTGAAGGGAATCAAGGGTCGTCTGAACCGGTTGCCGTCGGCTTGTGTGGGTGACATGGTCATGGCCACTGTGAAGAAAGGAAAGCCGGACCTCAGGAAGAAGGTGCTGCCAGCTGTCATTGTGAGGCAGCGCAAGCCGTGGCGCCGAAAGGATGGTGTTTTCATGTACTTCGAAGATAATGCTGGTGTCATTGTGAATCCAAAGGGTGAAATGAAAGGATCTGCTATCACTGGGCCTATTGGGAAGGAATGCGCTGATCTGTGGCCAAGAATTGCTAGTGCAGCTAATGCCATAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14

Protein Analysis

140

Amino Acids

15.03

Weight (kDa)

10.37

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 21 - 140 1.2e-41 Ribosomal protein L14p/L23e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04480 AT2G33370 AT3G04400
pyrus_communis pycom13g19490
rosa_wichuraiana Rw7G016970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 98, 264
AccII CGCG 1 cut(s) 18
AciI CCGC 1 cut(s) 18
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 2 cut(s) 186, 386
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 1 cut(s) 290
AgeI ACCGGT 2 cut(s) 95, 150
AluBI AGCT 2 cut(s) 236, 407
AluI AGCT 2 cut(s) 236, 407
AlwI GGATC 1 cut(s) 349
AoxI GGCC 3 cut(s) 186, 356, 386
ApeKI GCWGC 4 cut(s) 68, 229, 250, 404
AsiGI ACCGGT 2 cut(s) 95, 150
AspLEI GCGC 5 cut(s) 18, 86, 255, 267, 377
AspS9I GGNCC 2 cut(s) 211, 356
AsuHPI GGTGA 3 cut(s) 88, 185, 341
AsuII TTCGAA 1 cut(s) 294
AvaII GGWCC 1 cut(s) 211
AxyI CCTNAGG 1 cut(s) 215
BalI TGGCCA 2 cut(s) 188, 388
BanI GGYRCC 2 cut(s) 98, 264
BbvI GCAGC 4 cut(s) 80, 216, 262, 416
BccI CCATC 1 cut(s) 269
BceAI ACGGC 2 cut(s) 142, 244
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BfaI CTAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 268
BisI GCNGC 4 cut(s) 69, 230, 251, 405
BlsI GCNGC 4 cut(s) 70, 231, 252, 406
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 211, 356
BmiI GGNNCC 2 cut(s) 100, 266
BmrI ACTGGG 1 cut(s) 363
BmuI ACTGGG 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 63, 260
BsaWI WCCGGW 2 cut(s) 95, 150
Bse118I RCCGGY 2 cut(s) 95, 150
Bse1I ACTGG 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 215
BseDI CCNNGG 2 cut(s) 63, 260
BseGI GGATG 2 cut(s) 51, 280
BseMII CTCAG 1 cut(s) 229
BseNI ACTGG 1 cut(s) 358
BseXI GCAGC 4 cut(s) 80, 216, 262, 416
BseYI CCCAGC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 18
BshFI GGCC 3 cut(s) 188, 358, 388
BshNI GGYRCC 2 cut(s) 98, 264
BshTI ACCGGT 2 cut(s) 95, 150
BsiSI CCGG 4 cut(s) 30, 96, 151, 209
BsmI GAATGC 1 cut(s) 377
BsnI GGCC 3 cut(s) 188, 358, 388
Bsp119I TTCGAA 1 cut(s) 294
Bsp143I GATC 2 cut(s) 341, 379
BspACI CCGC 1 cut(s) 18
BspANI GGCC 3 cut(s) 188, 358, 388
BspCNI CTCAG 1 cut(s) 228
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 2 cut(s) 100, 266
BspPI GGATC 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 294
BspT107I GGYRCC 2 cut(s) 98, 264
BsrFI RCCGGY 2 cut(s) 95, 150
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 2 cut(s) 95, 150
BssECI CCNNGG 2 cut(s) 63, 260
BssMI GATC 2 cut(s) 341, 379
BssNI GRCGYC 1 cut(s) 265
Bst4CI ACNGT 2 cut(s) 76, 193
BstACI GRCGYC 1 cut(s) 265
BstAPI GCANNNNNTGC 1 cut(s) 410
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 234, 257
BstDEI CTNAG 1 cut(s) 215
BstDSI CCRYGG 2 cut(s) 63, 260
BstF5I GGATG 2 cut(s) 51, 280
BstFNI CGCG 1 cut(s) 18
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 5 cut(s) 18, 86, 255, 267, 377
BstKTI GATC 2 cut(s) 344, 382
BstMBI GATC 2 cut(s) 341, 379
BstMWI GCNNNNNNNGC 4 cut(s) 58, 256, 404, 410
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 4 cut(s) 80, 216, 262, 416
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
Bsu36I CCTNAGG 1 cut(s) 215
BsuRI GGCC 3 cut(s) 188, 358, 388
BtgI CCRYGG 2 cut(s) 63, 260
BtsCI GGATG 2 cut(s) 51, 280
BtsIMutI CAGTG 2 cut(s) 189, 351
Cac8I GCNNGC 2 cut(s) 234, 257
CfoI GCGC 5 cut(s) 18, 86, 255, 267, 377
Cfr10I RCCGGY 2 cut(s) 95, 150
Cfr13I GGNCC 2 cut(s) 211, 356
Csp6I GTAC 1 cut(s) 289
CspAI ACCGGT 2 cut(s) 95, 150
CviAII CATG 3 cut(s) 178, 184, 286
CviJI RGCY 8 cut(s) 164, 188, 208, 236, 259, 358, 388, 407
CviKI_1 RGCY 8 cut(s) 164, 188, 208, 236, 259, 358, 388, 407
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 215
DinI GGCGCC 1 cut(s) 266
DpnI GATC 2 cut(s) 343, 381
DpnII GATC 2 cut(s) 341, 379
EaeI YGGCCR 2 cut(s) 186, 386
Eco47I GGWCC 1 cut(s) 211
Eco81I CCTNAGG 1 cut(s) 215
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
FaeI CATG 3 cut(s) 181, 187, 289
FaiI YATR 4 cut(s) 179, 185, 287, 416
FatI CATG 3 cut(s) 177, 183, 285
Fnu4HI GCNGC 4 cut(s) 69, 230, 251, 405
FokI GGATG 2 cut(s) 58, 287
Fsp4HI GCNGC 4 cut(s) 69, 230, 251, 405
FspBI CTAG 1 cut(s) 399
GlaI GCGC 5 cut(s) 17, 85, 254, 266, 376
GluI GCNGC 4 cut(s) 69, 230, 251, 405
GsaI CCCAGC 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 3 cut(s) 188, 358, 388
HapII CCGG 4 cut(s) 30, 96, 151, 209
HhaI GCGC 5 cut(s) 18, 86, 255, 267, 377
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 3 cut(s) 181, 187, 289
Hin6I GCGC 5 cut(s) 16, 84, 253, 265, 375
HinP1I GCGC 5 cut(s) 16, 84, 253, 265, 375
HinfI GANTC 3 cut(s) 106, 132, 319
HpaII CCGG 4 cut(s) 30, 96, 151, 209
HphI GGTGA 3 cut(s) 88, 185, 341
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 2 cut(s) 43, 217
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 2 cut(s) 76, 163
HpyAV CCTTC 3 cut(s) 121, 217, 361
HpyCH4III ACNGT 2 cut(s) 76, 193
HpyCH4V TGCA 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 256, 404, 410
HpyF3I CTNAG 1 cut(s) 215
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 3 cut(s) 181, 187, 289
HspAI GCGC 5 cut(s) 16, 84, 253, 265, 375
KasI GGCGCC 1 cut(s) 264
Kzo9I GATC 2 cut(s) 341, 379
Lsp1109I GCAGC 4 cut(s) 80, 216, 262, 416
MaeI CTAG 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 173
MalI GATC 2 cut(s) 343, 381
MboI GATC 2 cut(s) 341, 379
MboII GAAGA 3 cut(s) 208, 232, 308
MflI RGATCY 1 cut(s) 341
MlsI TGGCCA 2 cut(s) 188, 388
MluCI AATT 1 cut(s) 393
MluNI TGGCCA 2 cut(s) 188, 388
Mly113I GGCGCC 1 cut(s) 265
MnlI CCTC 4 cut(s) 5, 14, 224, 240
Mox20I TGGCCA 2 cut(s) 188, 388
MscI TGGCCA 2 cut(s) 188, 388
MseI TTAA 1 cut(s) 421
MslI CAYNNNNRTG 1 cut(s) 182
Msp20I TGGCCA 2 cut(s) 188, 388
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 4 cut(s) 30, 96, 151, 209
Mva1269I GAATGC 1 cut(s) 377
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 4 cut(s) 58, 256, 404, 410
NarI GGCGCC 1 cut(s) 265
NdeII GATC 2 cut(s) 341, 379
NlaIII CATG 3 cut(s) 181, 187, 289
NlaIV GGNNCC 2 cut(s) 100, 266
NmuCI GTSAC 1 cut(s) 173
NspV TTCGAA 1 cut(s) 294
PctI GAATGC 1 cut(s) 377
PfeI GAWTC 3 cut(s) 106, 132, 319
PflFI GACNNNGTC 1 cut(s) 179
PinAI ACCGGT 2 cut(s) 95, 150
PkrI GCNGC 4 cut(s) 70, 231, 252, 406
PluTI GGCGCC 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 100, 266
PspPI GGNCC 2 cut(s) 211, 356
PsuI RGATCY 1 cut(s) 341
PsyI GACNNNGTC 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 182
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 4 cut(s) 69, 230, 251, 405
Sau3AI GATC 2 cut(s) 341, 379
Sau96I GGNCC 2 cut(s) 211, 356
SetI ASST 4 cut(s) 216, 228, 238, 409
SfoI GGCGCC 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 294
SgrAI CRCCGGYG 1 cut(s) 95
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 182
Sse9I AATT 1 cut(s) 393
SsiI CCGC 1 cut(s) 18
SspDI GGCGCC 1 cut(s) 264
SspMI CTAG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 76, 193
TaqI TCGA 2 cut(s) 5, 294
TasI AATT 1 cut(s) 393
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 3 cut(s) 106, 132, 319
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TscAI CASTG 2 cut(s) 196, 358
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 4 cut(s) 68, 229, 250, 404
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 274, 350
TspRI CASTG 2 cut(s) 196, 358
Tth111I GACNNNGTC 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 211
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.