AT3G04400

60S ribosomal protein L23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0020844
Physical Location & Seq
Forward (+)
1167250 .. 1168723
1474 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04400.1

Sequence Viewer

Length: 423 bp
ATGTCGAAGCGAGGAAGAGGAGGAACCTCTGGTAACAAGTTCAGGATGTCACTGGGTCTTCCAGTGGCAGCCACTGTGAACTGTGCTGACAACACCGGAGCTAAGAACCTTTACATCATTTCGGTTAAAGGAATCAAGGGTCGTCTTAACCGTTTGCCATCAGCGTGTGTTGGTGATATGGTTATGGCTACTGTTAAGAAAGGTAAGCCTGATCTCCGTAAGAAGGTGCTTCCTGCTGTCATTGTTAGGCAGAGGAAGCCTTGGCGCCGAAAGGACGGTGTCTTCATGTATTTCGAAGATAATGCTGGTGTCATTGTCAATCCCAAGGGTGAAATGAAAGGATCTGCTATCACTGGACCAATCGGAAAGGAGTGTGCTGATCTGTGGCCAAGAATCGCAAGTGCTGCGAACGCCATCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14

Protein Analysis

140

Amino Acids

15.03

Weight (kDa)

10.48

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 21 - 140 1.2e-41 Ribosomal protein L14p/L23e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020844)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04480 AT2G33370 AT3G04400
pyrus_communis pycom13g19490
rosa_wichuraiana Rw7G016970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 264
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 1 cut(s) 386
AcyI GRCGYC 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 223
AluBI AGCT 1 cut(s) 101
AluI AGCT 1 cut(s) 101
AlwI GGATC 1 cut(s) 349
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 2 cut(s) 68, 404
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 1 cut(s) 356
AsuHPI GGTGA 2 cut(s) 185, 341
AsuII TTCGAA 1 cut(s) 294
AvaII GGWCC 1 cut(s) 356
BalI TGGCCA 1 cut(s) 388
BanI GGYRCC 1 cut(s) 264
BbsI GAAGAC 2 cut(s) 50, 274
BbvI GCAGC 2 cut(s) 80, 391
BccI CCATC 2 cut(s) 166, 422
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BfoI RGCGCY 1 cut(s) 268
BisI GCNGC 2 cut(s) 69, 405
BlsI GCNGC 2 cut(s) 70, 406
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 2 cut(s) 25, 266
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
BpiI GAAGAC 2 cut(s) 50, 274
Bpu14I TTCGAA 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 2 cut(s) 260, 324
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 3 cut(s) 57, 62, 358
BseDI CCNNGG 2 cut(s) 260, 324
BseGI GGATG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 223
BseNI ACTGG 3 cut(s) 57, 62, 358
BseRI GAGGAG 1 cut(s) 33
BseXI GCAGC 2 cut(s) 80, 391
BshFI GGCC 1 cut(s) 388
BshNI GGYRCC 1 cut(s) 264
BsiSI CCGG 1 cut(s) 96
BslI CCNNNNNNNGG 1 cut(s) 223
BsnI GGCC 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 294
Bsp143I GATC 3 cut(s) 211, 341, 379
BspANI GGCC 1 cut(s) 388
BspLI GGNNCC 2 cut(s) 25, 266
BspPI GGATC 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 294
BspT107I GGYRCC 1 cut(s) 264
BsrI ACTGG 3 cut(s) 57, 62, 358
BssECI CCNNGG 2 cut(s) 260, 324
BssMI GATC 3 cut(s) 211, 341, 379
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 2 cut(s) 260, 324
Bst4CI ACNGT 5 cut(s) 76, 83, 152, 193, 278
Bst6I CTCTTC 1 cut(s) 10
BstACI GRCGYC 1 cut(s) 265
BstAPI GCANNNNNTGC 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 294
BstDEI CTNAG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 3 cut(s) 214, 344, 382
BstMBI GATC 3 cut(s) 211, 341, 379
BstMWI GCNNNNNNNGC 3 cut(s) 256, 404, 410
BstV1I GCAGC 2 cut(s) 80, 391
BstV2I GAAGAC 2 cut(s) 50, 274
BstX2I RGATCY 1 cut(s) 341
BstYI RGATCY 1 cut(s) 341
BsuRI GGCC 1 cut(s) 388
BtsCI GGATG 1 cut(s) 51
BtsIMutI CAGTG 4 cut(s) 50, 69, 72, 351
CaiI CAGNNNCTG 1 cut(s) 74
CfoI GCGC 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 356
CviAII CATG 1 cut(s) 286
CviJI RGCY 6 cut(s) 71, 101, 188, 208, 259, 388
CviKI_1 RGCY 6 cut(s) 71, 101, 188, 208, 259, 388
DdeI CTNAG 1 cut(s) 102
DinI GGCGCC 1 cut(s) 266
DpnI GATC 3 cut(s) 213, 343, 381
DpnII GATC 3 cut(s) 211, 341, 379
EaeI YGGCCR 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 10
EarI CTCTTC 1 cut(s) 10
Eco130I CCWWGG 2 cut(s) 260, 324
Eco47I GGWCC 1 cut(s) 356
EcoT14I CCWWGG 2 cut(s) 260, 324
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
ErhI CCWWGG 2 cut(s) 260, 324
FaeI CATG 1 cut(s) 289
FaiI YATR 3 cut(s) 179, 185, 287
FatI CATG 1 cut(s) 285
Fnu4HI GCNGC 2 cut(s) 69, 405
FokI GGATG 1 cut(s) 58
Fsp4HI GCNGC 2 cut(s) 69, 405
GlaI GCGC 1 cut(s) 266
GluI GCNGC 2 cut(s) 69, 405
HaeII RGCGCY 1 cut(s) 268
HaeIII GGCC 1 cut(s) 388
HapII CCGG 1 cut(s) 96
HhaI GCGC 1 cut(s) 267
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 1 cut(s) 289
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HinfI GANTC 2 cut(s) 132, 393
HpaII CCGG 1 cut(s) 96
HphI GGTGA 2 cut(s) 185, 341
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 365
Hpy188III TCNNGA 1 cut(s) 43
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 5 cut(s) 76, 83, 152, 193, 278
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 404, 410
HpyF3I CTNAG 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 1 cut(s) 289
HspAI GCGC 1 cut(s) 265
KasI GGCGCC 1 cut(s) 264
Kzo9I GATC 3 cut(s) 211, 341, 379
LmnI GCTCC 1 cut(s) 98
LpnPI CCDG 9 cut(s) 15, 28, 38, 75, 109, 222, 246, 291, 339
Lsp1109I GCAGC 2 cut(s) 80, 391
MaeIII GTNAC 2 cut(s) 32, 48
MalI GATC 3 cut(s) 213, 343, 381
MboI GATC 3 cut(s) 211, 341, 379
MboII GAAGA 4 cut(s) 27, 50, 274, 308
MflI RGATCY 1 cut(s) 341
MlsI TGGCCA 1 cut(s) 388
MluNI TGGCCA 1 cut(s) 388
Mly113I GGCGCC 1 cut(s) 265
MnlI CCTC 5 cut(s) 5, 11, 14, 37, 246
Mox20I TGGCCA 1 cut(s) 388
MscI TGGCCA 1 cut(s) 388
MseI TTAA 4 cut(s) 126, 147, 195, 421
MslI CAYNNNNRTG 1 cut(s) 163
Msp20I TGGCCA 1 cut(s) 388
MspI CCGG 1 cut(s) 96
MwoI GCNNNNNNNGC 3 cut(s) 256, 404, 410
NarI GGCGCC 1 cut(s) 265
NdeII GATC 3 cut(s) 211, 341, 379
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 2 cut(s) 25, 266
NmuCI GTSAC 1 cut(s) 48
NspV TTCGAA 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 148
PfeI GAWTC 2 cut(s) 132, 393
PflFI GACNNNGTC 1 cut(s) 278
PkrI GCNGC 2 cut(s) 70, 406
PluTI GGCGCC 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 25, 266
PspPI GGNCC 1 cut(s) 356
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 341
PsyI GACNNNGTC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 163
SaqAI TTAA 4 cut(s) 126, 147, 195, 421
SatI GCNGC 2 cut(s) 69, 405
Sau3AI GATC 3 cut(s) 211, 341, 379
Sau96I GGNCC 1 cut(s) 356
SetI ASST 5 cut(s) 29, 103, 111, 205, 228
SfoI GGCGCC 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 294
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 163
SspDI GGCGCC 1 cut(s) 264
StyI CCWWGG 2 cut(s) 260, 324
TaaI ACNGT 5 cut(s) 76, 83, 152, 193, 278
TaqI TCGA 2 cut(s) 5, 294
TfiI GAWTC 2 cut(s) 132, 393
Tru1I TTAA 4 cut(s) 126, 147, 195, 421
Tru9I TTAA 4 cut(s) 126, 147, 195, 421
TscAI CASTG 4 cut(s) 57, 69, 79, 358
TseFI GTSAC 1 cut(s) 48
TseI GCWGC 2 cut(s) 68, 404
Tsp45I GTSAC 1 cut(s) 48
TspDTI ATGAA 2 cut(s) 274, 350
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 4 cut(s) 57, 69, 79, 358
Tth111I GACNNNGTC 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.