AT1G14030

Rubisco LSMT substrate-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010211
Physical Location & Seq
Reverse (-)
4805306 .. 4807517
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14030.1

Sequence Viewer

Length: 1449 bp
ATGTCAGCCTCCGTCGCCGTCGTGTCGGGTTTTCTCCGTATCCCGTCAATTCAAAAGTCTCAAAACCCTTCCTTTCTCTTTTCTCGACCCAAGAAATCTTTGGTGAGACCCATTTCAGCTTCAAGCTCAGAGTTGCCGGAAAATGTTCGGAATTTCTGGAAATGGCTCAGAGACCAAGGAGTCGTATCCGGGAAGTCTGTGGCAGAACCTGCGGTGGTACCGGAAGGTTTGGGACTTGTAGCCAGGCGTGATATTGGAAGAAACGAGGTCGTATTGGAGATTCCCAAGCGATTGTGGATAAACCCAGAGACAGTGACTGCTTCCAAGATTGGACCTTTATGCGGCGGATTAAAGCCGTGGGTTTCAGTAGCTCTGTTTTTGATCAGAGAGAAGTATGAAGAAGAGTCTTCATGGAGAGTTTATCTTGATATGCTTCCTCAATCTACTGATTCTACTGTCTTCTGGTCAGAAGAGGAGCTTGCTGAGCTTAAAGGGACTCAACTGTTGAGCACCACATTGGGTGTGAAAGAGTATGTGGAGAATGAATTCTTGAAACTGGAACAAGAGATATTACTGCCTAACAAAGATCTCTTCTCATCCCGCATAACACTTGATGACTTCATATGGGCGTTTGGGATCCTCAAGTCGAGGGCTTTTTCTCGTCTCCGTGGCCAAAACCTTGTCTTGATCCCTCTTGCAGACTTGATAAACCATAACCCCGCGATAAAGACAGAAGATTATGCATACGAGATCAAAGGAGCCGGCCTTTTCTCTAGAGATCTCTTATTTTCCTTGAAGTCACCTGTTTATGTTAAAGCAGGTGAGCAGGTATACATTCAGTACGATCTGAACAAAAGCAATGCAGAACTTGCTCTCGACTATGGTTTTGTGGAATCAAACCCTAAACGGAACTCATATACTTTAACAATAGAGATACCAGAATCAGACCCATTCTTTGGGGATAAGTTGGATATTGCTGAGAGTAACAAGATGGGTGAGACCGGATACTTTGACATAGTAGACGGCCAGACTCTTCCCGCTGGTATGCTTCAGTACCTTCGGCTTGTGGCTCTTGGCGGTCCAGATGCTTTCTTATTAGAATCTATCTTCAATAACACCATATGGGGTCATCTTGAATTGCCTGTAAGTCGTACAAACGAGGAACTCATATGCCGTGTTGTCAGAGATGCCTGCAAATCTGCTCTGTCTGGTTTTGATACGACCATTGAAGAGGATGAGAAGCTTCTGGACAAAGGAAAGCTTGAGCCTAGGTTGGAAATGGCTCTCAAGATAAGGATTGGTGAGAAGAGAGTGCTTCAGCAAATCGACCAAATCTTCAAGGATAGAGAGCTTGAACTTGACATTTTAGAGTATTACCAAGAGAGAAGGCTCAAAGATCTTGGGTTGGTTGGCGAACAAGGGGATATTATCTTCTGGGAAACCAAGTGA

Protein Analysis

482

Amino Acids

54.61

Weight (kDa)

5.02

Isoelectric Point (pI)

44.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 313 - 439 1.9e-34 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14030
fragaria_vesca FvH4_4g30820 FvH4_4g30820 FvH4_4g30820
malus_domestica MD16G1067900.v1.1
prunus_persica Prupe.1G278900_v2.0.a1
pyrus_communis pycom13g06090 pycom16g05950
rosa_chinensis RchiOBHm_Chr4g0439211
rosa_laevigata RLG00000006287
rosa_multiflora Rmu_sc0002236.1_g000013 Rmu_sc0006598.1_g000019
rosa_roxburghii Rroxscaffold_5G00380200
rosa_rugosa Rorug04G0317100
rosa_samantha Rh4AG367600 Rh4BG379200 Rh4CG393600 Rh4DG373800
rosa_wichuraiana Rw0G005650 Rw0G011640 Rw5G028540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 809
AasI GACNNNNNNGTC 1 cut(s) 179
Acc36I ACCTGC 3 cut(s) 217, 809, 817
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 956
AccI GTMKAC 2 cut(s) 831, 1020
AccII CGCG 1 cut(s) 722
AciI CCGC 7 cut(s) 212, 342, 345, 601, 720, 1038, 1077
AclWI GGATC 3 cut(s) 631, 644, 682
AcoI YGGCCR 2 cut(s) 670, 1024
AcsI RAATTY 2 cut(s) 151, 545
AcuI CTGAAG 2 cut(s) 1034, 1301
AfaI GTAC 4 cut(s) 219, 842, 1055, 1153
AfiI CCNNNNNNNGG 2 cut(s) 341, 956
AgsI TTSAA 9 cut(s) 53, 123, 553, 796, 1111, 1136, 1229, 1339, 1355
AjnI CCWGG 1 cut(s) 242
AluBI AGCT 8 cut(s) 119, 126, 371, 478, 487, 1243, 1261, 1351
AluI AGCT 8 cut(s) 119, 126, 371, 478, 487, 1243, 1261, 1351
Alw21I GWGCWC 1 cut(s) 512
Alw26I GTCTC 6 cut(s) 63, 100, 165, 302, 668, 992
AlwI GGATC 3 cut(s) 631, 644, 682
AlwNI CAGNNNCTG 2 cut(s) 209, 317
AoxI GGCC 3 cut(s) 670, 763, 1024
ApoI RAATTY 2 cut(s) 151, 545
ArsI GACNNNNNNTTYG 4 cut(s) 869, 901, 938, 970
Asp700I GAANNNNTTC 2 cut(s) 144, 545
Asp718I GGTACC 1 cut(s) 217
AspA2I CCTAGG 1 cut(s) 1268
AspS9I GGNCC 2 cut(s) 332, 1079
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 5 cut(s) 115, 792, 833, 1007, 1313
AvaII GGWCC 2 cut(s) 332, 1079
AvrII CCTAGG 1 cut(s) 1268
BalI TGGCCA 1 cut(s) 672
BamHI GGATCC 1 cut(s) 636
BanI GGYRCC 1 cut(s) 217
BbsI GAAGAC 2 cut(s) 399, 451
Bbv12I GWGCWC 1 cut(s) 512
BccI CCATC 1 cut(s) 985
BceAI ACGGC 4 cut(s) 2, 340, 1039, 1158
BciT130I CCWGG 1 cut(s) 244
BciVI GTATCC 3 cut(s) 50, 196, 998
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 6 cut(s) 63, 100, 165, 302, 668, 992
BfaI CTAG 2 cut(s) 774, 1269
BfuAI ACCTGC 3 cut(s) 217, 809, 817
BfuI GTATCC 3 cut(s) 50, 196, 998
BglII AGATCT 3 cut(s) 586, 778, 1396
BisI GCNGC 1 cut(s) 343
BlnI CCTAGG 1 cut(s) 1268
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 1 cut(s) 344
Bme1390I CCNGG 2 cut(s) 190, 244
Bme18I GGWCC 2 cut(s) 332, 1079
BmgT120I GGNCC 2 cut(s) 332, 1079
BmiI GGNNCC 3 cut(s) 219, 638, 760
BmrFI CCNGG 2 cut(s) 190, 244
BmsI GCATC 2 cut(s) 1075, 1177
BpiI GAAGAC 2 cut(s) 399, 451
Bpu1102I GCTNAGC 1 cut(s) 483
BpuEI CTTGAG 3 cut(s) 626, 1271, 1283
BpuMI CCSGG 1 cut(s) 190
BsaI GGTCTC 3 cut(s) 100, 165, 992
BsaJI CCNNGG 4 cut(s) 175, 356, 667, 1268
BsaWI WCCGGW 2 cut(s) 220, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 956
Bse118I RCCGGY 1 cut(s) 761
Bse1I ACTGG 1 cut(s) 561
Bse3DI GCAATG 1 cut(s) 865
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 4 cut(s) 175, 356, 667, 1268
BseGI GGATG 2 cut(s) 596, 1240
BseLI CCNNNNNNNGG 2 cut(s) 341, 956
BseMI GCAATG 1 cut(s) 865
BseMII CTCAG 4 cut(s) 141, 181, 474, 969
BseNI ACTGG 1 cut(s) 561
BseRI GAGGAG 1 cut(s) 488
Bsh1236I CGCG 1 cut(s) 722
BshFI GGCC 3 cut(s) 672, 765, 1026
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 512
BsiSI CCGG 5 cut(s) 137, 189, 221, 762, 1002
BslFI GGGAC 2 cut(s) 246, 508
BslI CCNNNNNNNGG 2 cut(s) 341, 956
BsmAI GTCTC 6 cut(s) 63, 100, 165, 302, 668, 992
BsmBI CGTCTC 1 cut(s) 668
BsmFI GGGAC 2 cut(s) 246, 508
BsnI GGCC 3 cut(s) 672, 765, 1026
Bso31I GGTCTC 3 cut(s) 100, 165, 992
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 7 cut(s) 212, 342, 345, 601, 720, 1038, 1077
BspANI GGCC 3 cut(s) 672, 765, 1026
BspCNI CTCAG 4 cut(s) 140, 180, 475, 970
BspFNI CGCG 1 cut(s) 722
BspLI GGNNCC 3 cut(s) 219, 638, 760
BspMI ACCTGC 3 cut(s) 217, 809, 817
BspPI GGATC 3 cut(s) 631, 644, 682
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 3 cut(s) 100, 165, 992
BsrDI GCAATG 1 cut(s) 865
BsrFI RCCGGY 1 cut(s) 761
BsrI ACTGG 1 cut(s) 561
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 4 cut(s) 175, 356, 667, 1268
BssMI GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
BssNAI GTATAC 1 cut(s) 832
BssT1I CCWWGG 2 cut(s) 175, 1268
Bst1107I GTATAC 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 3 cut(s) 313, 457, 504
Bst6I CTCTTC 6 cut(s) 396, 465, 596, 1038, 1224, 1301
BstAPI GCANNNNNTGC 2 cut(s) 209, 869
BstC8I GCNNGC 3 cut(s) 480, 763, 1192
BstDEI CTNAG 4 cut(s) 127, 167, 483, 978
BstDSI CCRYGG 2 cut(s) 356, 667
BstF5I GGATG 2 cut(s) 596, 1240
BstFNI CGCG 1 cut(s) 722
BstKTI GATC 8 cut(s) 384, 589, 639, 690, 753, 781, 847, 1399
BstMAI GTCTC 6 cut(s) 63, 100, 165, 302, 668, 992
BstMBI GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
BstMWI GCNNNNNNNGC 4 cut(s) 14, 209, 484, 869
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 188, 242
BstUI CGCG 1 cut(s) 722
BstV2I GAAGAC 2 cut(s) 399, 451
BstX2I RGATCY 4 cut(s) 586, 636, 778, 1396
BstYI RGATCY 4 cut(s) 586, 636, 778, 1396
BstZ17I GTATAC 1 cut(s) 832
BsuI GTATCC 3 cut(s) 50, 196, 998
BsuRI GGCC 3 cut(s) 672, 765, 1026
BtgI CCRYGG 2 cut(s) 356, 667
BtsCI GGATG 2 cut(s) 596, 1240
BtsIMutI CAGTG 1 cut(s) 318
BveI ACCTGC 3 cut(s) 217, 809, 817
Cac8I GCNNGC 3 cut(s) 480, 763, 1192
CaiI CAGNNNCTG 2 cut(s) 209, 317
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 2 cut(s) 332, 1079
Csp6I GTAC 4 cut(s) 218, 841, 1054, 1152
CviAII CATG 1 cut(s) 411
CviQI GTAC 4 cut(s) 218, 841, 1054, 1152
DdeI CTNAG 4 cut(s) 127, 167, 483, 978
DpnI GATC 8 cut(s) 383, 588, 638, 689, 752, 780, 846, 1398
DpnII GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 2 cut(s) 670, 1024
Eam1104I CTCTTC 6 cut(s) 396, 465, 596, 1038, 1224, 1301
EarI CTCTTC 6 cut(s) 396, 465, 596, 1038, 1224, 1301
EciI GGCGGA 1 cut(s) 360
Eco130I CCWWGG 2 cut(s) 175, 1268
Eco31I GGTCTC 3 cut(s) 100, 165, 992
Eco47I GGWCC 2 cut(s) 332, 1079
Eco57I CTGAAG 2 cut(s) 1034, 1301
EcoRI GAATTC 1 cut(s) 545
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 2 cut(s) 175, 1268
EcoT22I ATGCAT 1 cut(s) 745
ErhI CCWWGG 2 cut(s) 175, 1268
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 1 cut(s) 414
FalI AAGNNNNNCTT 4 cut(s) 462, 494, 1245, 1277
FaqI GGGAC 2 cut(s) 246, 508
FatI CATG 1 cut(s) 410
FauI CCCGC 3 cut(s) 608, 727, 1045
FauNDI CATATG 3 cut(s) 623, 1121, 1169
FbaI TGATCA 1 cut(s) 381
FblI GTMKAC 2 cut(s) 831, 1020
Fnu4HI GCNGC 1 cut(s) 343
FokI GGATG 2 cut(s) 583, 1247
Fsp4HI GCNGC 1 cut(s) 343
FspBI CTAG 2 cut(s) 774, 1269
GluI GCNGC 1 cut(s) 343
HaeIII GGCC 3 cut(s) 672, 765, 1026
HapII CCGG 5 cut(s) 137, 189, 221, 762, 1002
Hin1II CATG 1 cut(s) 414
HindIII AAGCTT 2 cut(s) 1241, 1259
HinfI GANTC 9 cut(s) 180, 280, 404, 449, 496, 893, 941, 1030, 1100
HpaII CCGG 5 cut(s) 137, 189, 221, 762, 1002
HphI GGTGA 5 cut(s) 115, 792, 833, 1007, 1313
Hpy166II GTNNAC 2 cut(s) 832, 1021
Hpy188I TCNGA 8 cut(s) 130, 150, 170, 386, 469, 849, 946, 1184
Hpy8I GTNNAC 2 cut(s) 832, 1021
Hpy99I CGWCG 2 cut(s) 17, 23
HpyAV CCTTC 4 cut(s) 78, 218, 1067, 1380
HpyCH4III ACNGT 3 cut(s) 313, 457, 504
HpyCH4V TGCA 4 cut(s) 698, 743, 863, 1194
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 209, 484, 869
HpyF3I CTNAG 4 cut(s) 127, 167, 483, 978
Hsp92II CATG 1 cut(s) 414
KpnI GGTACC 1 cut(s) 221
KroI GCCGGC 1 cut(s) 761
KroNI GCCGGC 1 cut(s) 763
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
LmnI GCTCC 2 cut(s) 475, 758
LweI GCATC 2 cut(s) 1075, 1177
MaeI CTAG 2 cut(s) 774, 1269
MaeIII GTNAC 3 cut(s) 313, 798, 983
MalI GATC 8 cut(s) 383, 588, 638, 689, 752, 780, 846, 1398
MboI GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
MflI RGATCY 4 cut(s) 586, 636, 778, 1396
MhlI GDGCHC 1 cut(s) 512
MlsI TGGCCA 1 cut(s) 672
MluCI AATT 4 cut(s) 48, 151, 545, 1136
MluNI TGGCCA 1 cut(s) 672
MlyI GAGTC 4 cut(s) 189, 413, 490, 1024
MmeI TCCRAC 2 cut(s) 948, 1254
MnlI CCTC 9 cut(s) 19, 259, 447, 466, 642, 650, 702, 1153, 1225
Mox20I TGGCCA 1 cut(s) 672
Mph1103I ATGCAT 1 cut(s) 745
MroNI GCCGGC 1 cut(s) 761
MroXI GAANNNNTTC 2 cut(s) 144, 545
MscI TGGCCA 1 cut(s) 672
MseI TTAA 4 cut(s) 350, 489, 813, 923
Msp20I TGGCCA 1 cut(s) 672
MspA1I CMGCKG 1 cut(s) 1040
MspI CCGG 5 cut(s) 137, 189, 221, 762, 1002
MspR9I CCNGG 2 cut(s) 190, 244
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 1 cut(s) 722
MwoI GCNNNNNNNGC 4 cut(s) 14, 209, 484, 869
NaeI GCCGGC 1 cut(s) 763
NciI CCSGG 1 cut(s) 190
NdeI CATATG 3 cut(s) 623, 1121, 1169
NdeII GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
NgoMIV GCCGGC 1 cut(s) 761
NlaIII CATG 1 cut(s) 414
NlaIV GGNNCC 3 cut(s) 219, 638, 760
NmuCI GTSAC 2 cut(s) 313, 798
NsiI ATGCAT 1 cut(s) 745
PaqCI CACCTGC 1 cut(s) 809
PdiI GCCGGC 1 cut(s) 763
PdmI GAANNNNTTC 2 cut(s) 144, 545
PfeI GAWTC 5 cut(s) 280, 449, 893, 941, 1100
PflMI CCANNNNNTGG 1 cut(s) 956
PfoI TCCNGGA 1 cut(s) 188
PkrI GCNGC 1 cut(s) 344
PleI GAGTC 4 cut(s) 188, 412, 490, 1024
PpsI GAGTC 4 cut(s) 188, 412, 490, 1024
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 219, 638, 760
PspPI GGNCC 2 cut(s) 332, 1079
PstNI CAGNNNCTG 2 cut(s) 209, 317
PsuI RGATCY 4 cut(s) 586, 636, 778, 1396
RsaI GTAC 4 cut(s) 219, 842, 1055, 1153
RsaNI GTAC 4 cut(s) 218, 841, 1054, 1152
SaqAI TTAA 4 cut(s) 350, 489, 813, 923
SatI GCNGC 1 cut(s) 343
Sau3AI GATC 8 cut(s) 381, 586, 636, 687, 750, 778, 844, 1396
Sau96I GGNCC 2 cut(s) 332, 1079
SchI GAGTC 4 cut(s) 189, 413, 490, 1024
ScrFI CCNGG 2 cut(s) 190, 244
SduI GDGCHC 1 cut(s) 512
SfaNI GCATC 2 cut(s) 1075, 1177
SinI GGWCC 2 cut(s) 332, 1079
SmlI CTYRAG 3 cut(s) 641, 1262, 1286
SmoI CTYRAG 3 cut(s) 641, 1262, 1286
Sse9I AATT 4 cut(s) 48, 151, 545, 1136
SsiI CCGC 7 cut(s) 212, 342, 345, 601, 720, 1038, 1077
SspMI CTAG 2 cut(s) 774, 1269
StyD4I CCNGG 2 cut(s) 188, 242
StyI CCWWGG 2 cut(s) 175, 1268
TaaI ACNGT 3 cut(s) 313, 457, 504
TaqI TCGA 4 cut(s) 85, 647, 876, 1326
TasI AATT 4 cut(s) 48, 151, 545, 1136
TauI GCSGC 1 cut(s) 345
TfiI GAWTC 5 cut(s) 280, 449, 893, 941, 1100
Tru1I TTAA 4 cut(s) 350, 489, 813, 923
Tru9I TTAA 4 cut(s) 350, 489, 813, 923
TscAI CASTG 1 cut(s) 318
TseFI GTSAC 2 cut(s) 313, 798
Tsp45I GTSAC 2 cut(s) 313, 798
TspDTI ATGAA 4 cut(s) 399, 411, 558, 610
TspGWI ACGGA 3 cut(s) 26, 656, 922
TspRI CASTG 1 cut(s) 318
Van91I CCANNNNNTGG 1 cut(s) 956
VpaK11BI GGWCC 2 cut(s) 332, 1079
XapI RAATTY 2 cut(s) 151, 545
XbaI TCTAGA 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmaJI CCTAGG 1 cut(s) 1268
XmiI GTMKAC 2 cut(s) 831, 1020
XmnI GAANNNNTTC 2 cut(s) 144, 545
XspI CTAG 2 cut(s) 774, 1269
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.