AT1G16540

Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005454
Physical Location & Seq
Forward (+)
5659370 .. 5665488
6119 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G16540.1

Sequence Viewer

Length: 2460 bp
ATGGAAGCATTTCTTAAGGAATTCGGAGATTATTATGGATACCCAGATGGTCCCAAGAACATTCAAGAGATCCGCGACACCGAATTCAAGAGATTAGATAAAGGTGTTGTATACTTGGATCATGCTGGTTCTACTTTGTATTCTGAGTTGCAGATGGAATATATTTTTAAGGACTTCACAAGCAATGTTTTTGGAAATCCACATAGTCAAAGTGATATCAGTTCGGCCACCAGTGACCTTATAGCGGATGCTCGACATCAGGTGCTTGAATACTTTAATGCATCTCCTGAAGATTACAGTTGCTTATTCACCTCCGGAGCCACAGCAGCGCTGAAGCTTGTCGGAGAGACTTTTCCGTGGACCCAAGACAGTAATTTTTTGTATACCATGGAGAATCACAACAGTGTACTTGGTATTAGGGAATATGCATTAGCTCAAGGTGCTTCAGCATGTGCAGTGGATATTGAAGAGGCAGCTAACCAACCAGGCCAGCTTACAAATTCAGGACCATCTATCAAGGTAAAGCATCGTGCTGTGCAGATGAGAAACACTTCTAAACTCCAAAAGGAAGAGTCAAGAGGAAATGCCTATAATCTATTTGCTTTCCCCTCGGAGTGCAATTTTTCTGGCCTGAGGTTTAATCTAGATCTGGTGAAGTTGATGAAAGAAAATACTGAGACCGTGCTACAAGGCTCCCCCTTTAGCAAGAGCAAGCGGTGGATGGTCTTGATTGATGCTGCAAAGGGTTGTGCTACACTACCACCTGATTTATCGGAGTATCCTGCAGATTTTGTTGTTCTGTCATTCTACAAGTTATTTGGTTATCCTACTGGGCTTGGCGCTCTCCTTGTACGGAATGATGCAGCCAAATTGCTCAAAAAGACTTATTTTAGTGGAGGCACTGTTGCTGCTTCAATTGCTGACATCGACTTTGTAAAAAGAAGGGAAAGGGTGGAGGAGTTTTTTGAGGATGGTTCTGCTTCATTCCTGAGCATAGCAGCCATCCGTCATGGCTTCAAATTACTCAAGTCGCTTACACCTTCTGCAATTTGGATGCACACAACGTCACTTTCCATATATGTGAAAAAGAAGCTTCAGGCTTTACGACATGGAAACGGGGCTGCTGTATGTGTTCTGTATGGCAGTGAAAATCTGGAGTTATCTTCACATAAATCAGGCCCAACGGTTACATTCAACTTGAAAAGACCTGATGGCTCTTGGTTTGGCTACTTGGAGGTGGAGAAGCTTGCTTCTTTATCTGGAATTCAGTTACGGACAGGATGTTTTTGCAATCCTGGCGCATGTGCAAAGTATCTCGAGTTATCCCATTCTGAGCTACGGTCTAATGTAGAGGCTGGGCATATTTGCTGGGATGACAATGATGTGATAAATGGAAAACCAACAGGGGCTGTTAGGGTTTCGTTTGGTTATATGTCAACCTTTGAAGATGCCAAGAAATTTATTGATTTCATCATAAGTTCATTTGCTTCACCTCCAAAGAAGACTGGGAATGGAACCGTCGTCAGTGGAAGGTTTCCTCAACTTCCTAGTGAAGACCTTGAAAGTAAAGAATCTTTTCCAAGCCACTACCTTAAGTCAATTACTGTATACCCGATCAAGTCATGTGCTGGATTTTCTGTGATACGTTGGCCACTTTGCAGAACAGGCCTGCTGCATGATCGAGAATGGATGGTTCAGGGTCTGACCGGTGAAATTCTTACCCAAAAGAAGGTGCCTGAGATGTCTCTTATAAAAACCTTTATCGACCTTGAGGAAGGACTACTGTCTGTAGAATCTTCTCGCTGCGAAGACAAGTTGCACATCAGAATCAAGTCTGATTCATATAACCCGAGGAACGATGAGTTTGATTCACATGCCAACATACTTGAAAACCGTAATGAGGAAACTAGAATCAATCGTTGGTTCACCAATGCCATTGGTCGACAATGCAAGTTGCTACGGTATTCTAGCTCTACTTCCAAAGACTGCTTGAACAGAAACAAGAGTCCTGGTTTGTGCAGAGATTTGGAAAGCAATATCAACTTTGCTAATGAAGCTCAGTTCTTGTTAATCTCCGAGGAGAGTGTTGCTGACCTAAACAGAAGATTAGAAGCAAAAGACGAGGATTACAAACGGGCTCATGAAAAACTCAATCCACATAGGTTCAGACCAAATCTGGTTATATCTGGAGGTGAACCATACGGGGAAGATAAATGGAAAACTGTCAAGATAGGAGACAATCATTTCACATCATTGGGCGGTTGTAACCGGTGCCAGATGATAAACATAAGTAATGAAGCTGGACTAGTGAAGAAATCCAATGAGCCCTTAACAACTTTAGCTTCATATAGGAGAGTAAAGGGAAAGATCTTGTTTGGAACGCTTTTGAGATACGAGATTGATGAGAAAAGACAATGTTGGATTGGAGTTGGGGAAGAAGTTAATCCAGATATTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006066 GO:0006082 GO:0006464 GO:0006605 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008265 GO:0008299 GO:0008610 GO:0009000 GO:0009058 GO:0009266 GO:0009408 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009687 GO:0009688 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009743 GO:0009755 GO:0009756 GO:0009987 GO:0010033 GO:0010118 GO:0010182 GO:0015031 GO:0015833 GO:0016053 GO:0016106 GO:0016114 GO:0016740 GO:0016782 GO:0016783 GO:0016829 GO:0016846 GO:0017038 GO:0018315 GO:0019538 GO:0019752 GO:0023052 GO:0032787 GO:0032870 GO:0033036 GO:0033365 GO:0034613 GO:0036211 GO:0042040 GO:0042221 GO:0042742 GO:0042886 GO:0043170 GO:0043207 GO:0043288 GO:0043289 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0045036 GO:0045037 GO:0045184 GO:0046165 GO:0046394 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0065002 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071322 GO:0071365 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0072330 GO:0072594 GO:0072596 GO:0072598 GO:0098542 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902644 GO:1902645
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Aminotran_5 MOSC MOSC_N

Protein Analysis

819

Amino Acids

91.8

Weight (kDa)

6.56

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 37 - 154 1.9e-08 Aminotransferase class-V
Aminotran_5 PF00266 236 - 487 1e-16 Aminotransferase class-V
MOSC_N PF03476 530 - 649 1.6e-19 MOSC N-terminal beta barrel domain
MOSC PF03473 679 - 814 1.5e-22 MOSC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1751
AccB1I GGYRCC 2 cut(s) 1732, 2271
AccI GTMKAC 4 cut(s) 111, 383, 1608, 1942
AccII CGCG 1 cut(s) 75
AccIII TCCGGA 1 cut(s) 314
AciI CCGC 4 cut(s) 73, 245, 715, 2259
AclWI GGATC 2 cut(s) 64, 126
AcoI YGGCCR 2 cut(s) 225, 1649
AcsI RAATTY 6 cut(s) 20, 83, 499, 1263, 1457, 1713
AcuI CTGAAG 4 cut(s) 309, 353, 429, 1079
AfaI GTAC 2 cut(s) 408, 852
AfeI AGCGCT 1 cut(s) 330
AfiI CCNNNNNNNGG 3 cut(s) 244, 1729, 1900
AflII CTTAAG 2 cut(s) 14, 1592
AgeI ACCGGT 2 cut(s) 1706, 2268
AhlI ACTAGT 1 cut(s) 2305
AjnI CCWGG 3 cut(s) 484, 1294, 2008
AjuI GAANNNNNNNTTGG 2 cut(s) 1903, 1935
AleI CACNNNNGTG 1 cut(s) 402
Alw26I GTCTC 4 cut(s) 341, 671, 1749, 2229
AlwI GGATC 2 cut(s) 64, 126
AlwNI CAGNNNCTG 2 cut(s) 1409, 1702
Ama87I CYCGRG 2 cut(s) 1316, 1849
Aor13HI TCCGGA 1 cut(s) 314
Aor51HI AGCGCT 1 cut(s) 330
AoxI GGCC 6 cut(s) 225, 487, 628, 1177, 1649, 1666
ApeKI GCWGC 9 cut(s) 326, 473, 737, 863, 908, 998, 1121, 1672, 1803
ApoI RAATTY 6 cut(s) 20, 83, 499, 1263, 1457, 1713
ArsI GACNNNNNNTTYG 2 cut(s) 914, 946
AsiGI ACCGGT 2 cut(s) 1706, 2268
Asp700I GAANNNNTTC 3 cut(s) 9, 550, 1575
AspLEI GCGC 3 cut(s) 331, 842, 1301
AspS9I GGNCC 4 cut(s) 50, 360, 506, 1178
AsuHPI GGTGA 6 cut(s) 301, 664, 1482, 1721, 1918, 2204
AvaI CYCGRG 2 cut(s) 1316, 1849
AvaII GGWCC 3 cut(s) 50, 360, 506
AxyI CCTNAGG 1 cut(s) 632
BalI TGGCCA 1 cut(s) 1651
BanI GGYRCC 2 cut(s) 1732, 2271
BanII GRGCYC 2 cut(s) 2140, 2328
BbsI GAAGAC 3 cut(s) 1508, 1560, 1815
BbvI GCAGC 9 cut(s) 338, 485, 724, 875, 895, 1010, 1108, 1659, 1790
BccI CCATC 8 cut(s) 41, 148, 517, 715, 965, 1010, 1205, 1684
BciT130I CCWGG 3 cut(s) 486, 1296, 2010
BciVI GTATCC 2 cut(s) 32, 789
BcoDI GTCTC 4 cut(s) 341, 671, 1749, 2229
BcuI ACTAGT 1 cut(s) 2305
BfaI CTAG 5 cut(s) 644, 1548, 1908, 1968, 2306
BfmI CTRYAG 2 cut(s) 783, 1788
BfoI RGCGCY 2 cut(s) 332, 843
BfrI CTTAAG 2 cut(s) 14, 1592
BfuI GTATCC 2 cut(s) 32, 789
BglII AGATCT 2 cut(s) 646, 2367
BisI GCNGC 9 cut(s) 327, 474, 738, 864, 909, 999, 1122, 1673, 1804
BlsI GCNGC 9 cut(s) 328, 475, 739, 865, 910, 1000, 1123, 1674, 1805
Bme1390I CCNGG 3 cut(s) 486, 1296, 2010
Bme18I GGWCC 3 cut(s) 50, 360, 506
BmeT110I CYCGRG 2 cut(s) 1316, 1849
BmgT120I GGNCC 4 cut(s) 50, 360, 506, 1178
BmiI GGNNCC 7 cut(s) 52, 319, 362, 694, 1516, 1734, 2273
BmrFI CCNGG 3 cut(s) 486, 1296, 2010
BmrI ACTGGG 2 cut(s) 840, 1515
BmsI GCATC 7 cut(s) 238, 290, 535, 724, 850, 1044, 1438
BmuI ACTGGG 2 cut(s) 840, 1515
BoxI GACNNNNGTC 1 cut(s) 1783
BpiI GAAGAC 3 cut(s) 1508, 1560, 1815
BpmI CTGGAG 2 cut(s) 1175, 2208
Bpu10I CCTNAGC 1 cut(s) 989
BpuEI CTTGAG 3 cut(s) 420, 1010, 1790
BsaI GGTCTC 1 cut(s) 671
BsaJI CCNNGG 5 cut(s) 356, 387, 609, 1850, 2076
BsaWI WCCGGW 3 cut(s) 314, 1706, 2268
BsaXI ACNNNNNCTCC 2 cut(s) 888, 918
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 1729, 1900
Bse118I RCCGGY 2 cut(s) 1706, 2268
Bse1I ACTGG 3 cut(s) 231, 835, 1510
Bse21I CCTNAGG 1 cut(s) 632
Bse3DI GCAATG 1 cut(s) 190
BseAI TCCGGA 1 cut(s) 314
BseBI CCWGG 3 cut(s) 486, 1296, 2010
BseDI CCNNGG 5 cut(s) 356, 387, 609, 1850, 2076
BseGI GGATG 8 cut(s) 253, 726, 976, 1002, 1059, 1286, 1378, 1695
BseLI CCNNNNNNNGG 3 cut(s) 244, 1729, 1900
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 7 cut(s) 135, 623, 666, 980, 1323, 1728, 2072
BseNI ACTGG 3 cut(s) 231, 835, 1510
BseRI GAGGAG 2 cut(s) 971, 2093
BseXI GCAGC 9 cut(s) 338, 485, 724, 875, 895, 1010, 1108, 1659, 1790
BseYI CCCAGC 2 cut(s) 1355, 1368
BsgI GTGCAG 3 cut(s) 474, 557, 2038
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 6 cut(s) 227, 489, 630, 1179, 1651, 1668
BshNI GGYRCC 2 cut(s) 1732, 2271
BshTI ACCGGT 2 cut(s) 1706, 2268
BsiHKCI CYCGRG 2 cut(s) 1316, 1849
BsiSI CCGG 3 cut(s) 315, 1707, 2269
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 3 cut(s) 244, 1729, 1900
BsmAI GTCTC 4 cut(s) 341, 671, 1749, 2229
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 6 cut(s) 227, 489, 630, 1179, 1651, 1668
Bso31I GGTCTC 1 cut(s) 671
BsoBI CYCGRG 2 cut(s) 1316, 1849
Bsp1286I GDGCHC 2 cut(s) 2140, 2328
Bsp13I TCCGGA 1 cut(s) 314
Bsp143I GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 4 cut(s) 73, 245, 715, 2259
BspANI GGCC 6 cut(s) 227, 489, 630, 1179, 1651, 1668
BspCNI CTCAG 7 cut(s) 136, 624, 667, 981, 1324, 1729, 2071
BspEI TCCGGA 1 cut(s) 314
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 2140
BspLI GGNNCC 7 cut(s) 52, 319, 362, 694, 1516, 1734, 2273
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 2 cut(s) 64, 126
BspT107I GGYRCC 2 cut(s) 1732, 2271
BspTI CTTAAG 2 cut(s) 14, 1592
BspTNI GGTCTC 1 cut(s) 671
BsrDI GCAATG 1 cut(s) 190
BsrFI RCCGGY 2 cut(s) 1706, 2268
BsrI ACTGG 3 cut(s) 231, 835, 1510
BssAI RCCGGY 2 cut(s) 1706, 2268
BssECI CCNNGG 5 cut(s) 356, 387, 609, 1850, 2076
BssMI GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
BssNAI GTATAC 3 cut(s) 112, 384, 1609
BssT1I CCWWGG 1 cut(s) 387
Bst1107I GTATAC 3 cut(s) 112, 384, 1609
Bst2UI CCWGG 3 cut(s) 486, 1296, 2010
Bst6I CTCTTC 2 cut(s) 462, 564
BstAFI CTTAAG 2 cut(s) 14, 1592
BstC8I GCNNGC 4 cut(s) 491, 713, 1248, 1670
BstDEI CTNAG 7 cut(s) 144, 632, 675, 989, 1332, 1737, 2058
BstDSI CCRYGG 2 cut(s) 356, 387
BstF5I GGATG 8 cut(s) 253, 726, 976, 1002, 1059, 1286, 1378, 1695
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 2 cut(s) 332, 843
BstHHI GCGC 3 cut(s) 331, 842, 1301
BstKTI GATC 6 cut(s) 72, 121, 649, 1617, 1681, 2370
BstMAI GTCTC 4 cut(s) 341, 671, 1749, 2229
BstMBI GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
BstMWI GCNNNNNNNGC 6 cut(s) 326, 440, 917, 1296, 1665, 2054
BstNI CCWGG 3 cut(s) 486, 1296, 2010
BstNSI RCATGY 3 cut(s) 453, 1305, 1877
BstPAI GACNNNNGTC 1 cut(s) 1783
BstSCI CCNGG 3 cut(s) 484, 1294, 2008
BstSFI CTRYAG 2 cut(s) 783, 1788
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 9 cut(s) 338, 485, 724, 875, 895, 1010, 1108, 1659, 1790
BstV2I GAAGAC 3 cut(s) 1508, 1560, 1815
BstX2I RGATCY 3 cut(s) 69, 646, 2367
BstYI RGATCY 3 cut(s) 69, 646, 2367
BstZ17I GTATAC 3 cut(s) 112, 384, 1609
Bsu36I CCTNAGG 1 cut(s) 632
BsuI GTATCC 2 cut(s) 32, 789
BsuRI GGCC 6 cut(s) 227, 489, 630, 1179, 1651, 1668
BtgI CCRYGG 2 cut(s) 356, 387
BtsCI GGATG 8 cut(s) 253, 726, 976, 1002, 1059, 1286, 1378, 1695
BtsI GCAGTG 2 cut(s) 462, 1150
BtsIMutI CAGTG 6 cut(s) 238, 409, 462, 900, 1150, 1531
Cac8I GCNNGC 4 cut(s) 491, 713, 1248, 1670
CaiI CAGNNNCTG 2 cut(s) 1409, 1702
CciI TCATGA 1 cut(s) 2140
CfoI GCGC 3 cut(s) 331, 842, 1301
Cfr10I RCCGGY 2 cut(s) 1706, 2268
Cfr13I GGNCC 4 cut(s) 50, 360, 506, 1178
Csp6I GTAC 2 cut(s) 407, 851
CspAI ACCGGT 2 cut(s) 1706, 2268
CviQI GTAC 2 cut(s) 407, 851
DdeI CTNAG 7 cut(s) 144, 632, 675, 989, 1332, 1737, 2058
DpnI GATC 6 cut(s) 71, 120, 648, 1616, 1680, 2369
DpnII GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
EaeI YGGCCR 2 cut(s) 225, 1649
Eam1104I CTCTTC 2 cut(s) 462, 564
EarI CTCTTC 2 cut(s) 462, 564
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 1668
Eco24I GRGCYC 2 cut(s) 2140, 2328
Eco31I GGTCTC 1 cut(s) 671
Eco32I GATATC 1 cut(s) 217
Eco47I GGWCC 3 cut(s) 50, 360, 506
Eco47III AGCGCT 1 cut(s) 330
Eco57I CTGAAG 4 cut(s) 309, 353, 429, 1079
Eco81I CCTNAGG 1 cut(s) 632
Eco88I CYCGRG 2 cut(s) 1316, 1849
EcoRI GAATTC 3 cut(s) 20, 83, 1263
EcoRII CCWGG 3 cut(s) 484, 1294, 2008
EcoRV GATATC 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 2 cut(s) 283, 430
EcoT38I GRGCYC 2 cut(s) 2140, 2328
ErhI CCWWGG 1 cut(s) 387
FalI AAGNNNNNCTT 1 cut(s) 29
FaqI GGGAC 1 cut(s) 36
FblI GTMKAC 4 cut(s) 111, 383, 1608, 1942
Fnu4HI GCNGC 9 cut(s) 327, 474, 738, 864, 909, 999, 1122, 1673, 1804
FokI GGATG 8 cut(s) 260, 733, 983, 989, 1066, 1293, 1385, 1702
FriOI GRGCYC 2 cut(s) 2140, 2328
Fsp4HI GCNGC 9 cut(s) 327, 474, 738, 864, 909, 999, 1122, 1673, 1804
FspBI CTAG 5 cut(s) 644, 1548, 1908, 1968, 2306
GlaI GCGC 3 cut(s) 330, 841, 1300
GluI GCNGC 9 cut(s) 327, 474, 738, 864, 909, 999, 1122, 1673, 1804
GsaI CCCAGC 2 cut(s) 1359, 1372
GsuI CTGGAG 2 cut(s) 1175, 2208
HaeII RGCGCY 2 cut(s) 332, 843
HaeIII GGCC 6 cut(s) 227, 489, 630, 1179, 1651, 1668
HapII CCGG 3 cut(s) 315, 1707, 2269
HhaI GCGC 3 cut(s) 331, 842, 1301
Hin6I GCGC 3 cut(s) 329, 840, 1299
HinP1I GCGC 3 cut(s) 329, 840, 1299
HincII GTYRAC 2 cut(s) 1437, 1943
HindII GTYRAC 2 cut(s) 1437, 1943
HindIII AAGCTT 3 cut(s) 335, 1091, 1244
HinfI GANTC 9 cut(s) 394, 572, 1571, 1793, 1827, 1838, 1868, 1911, 2005
HpaII CCGG 3 cut(s) 315, 1707, 2269
HphI GGTGA 6 cut(s) 301, 664, 1482, 1721, 1918, 2204
Hpy166II GTNNAC 9 cut(s) 112, 360, 384, 407, 1437, 1609, 1926, 1943, 2195
Hpy8I GTNNAC 9 cut(s) 112, 360, 384, 407, 1437, 1609, 1926, 1943, 2195
Hpy99I CGWCG 1 cut(s) 1523
HpyAV CCTTC 5 cut(s) 936, 1050, 1524, 1723, 1769
HpyCH4IV ACGT 2 cut(s) 1064, 1645
HpyF10VI GCNNNNNNNGC 6 cut(s) 326, 440, 917, 1296, 1665, 2054
HpyF3I CTNAG 7 cut(s) 144, 632, 675, 989, 1332, 1737, 2058
HpySE526I ACGT 2 cut(s) 1064, 1645
HspAI GCGC 3 cut(s) 329, 840, 1299
Kpn2I TCCGGA 1 cut(s) 314
Kzo9I GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
LmnI GCTCC 2 cut(s) 317, 698
Lsp1109I GCAGC 9 cut(s) 338, 485, 724, 875, 895, 1010, 1108, 1659, 1790
LweI GCATC 7 cut(s) 238, 290, 535, 724, 850, 1044, 1438
MaeI CTAG 5 cut(s) 644, 1548, 1908, 1968, 2306
MaeII ACGT 2 cut(s) 1064, 1645
MaeIII GTNAC 5 cut(s) 233, 1065, 1186, 1269, 2264
MalI GATC 6 cut(s) 71, 120, 648, 1616, 1680, 2369
MboI GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 3 cut(s) 69, 646, 2367
MhlI GDGCHC 2 cut(s) 2140, 2328
MlsI TGGCCA 1 cut(s) 1651
MluNI TGGCCA 1 cut(s) 1651
MlyI GAGTC 2 cut(s) 581, 2014
MmeI TCCRAC 2 cut(s) 322, 2399
Mox20I TGGCCA 1 cut(s) 1651
Mph1103I ATGCAT 2 cut(s) 283, 430
MroI TCCGGA 1 cut(s) 314
MroXI GAANNNNTTC 3 cut(s) 9, 550, 1575
MscI TGGCCA 1 cut(s) 1651
MseI TTAA 8 cut(s) 15, 168, 276, 639, 1593, 2069, 2330, 2442
MslI CAYNNNNRTG 2 cut(s) 402, 1079
Msp20I TGGCCA 1 cut(s) 1651
MspCI CTTAAG 2 cut(s) 14, 1592
MspI CCGG 3 cut(s) 315, 1707, 2269
MspR9I CCNGG 3 cut(s) 486, 1296, 2010
MunI CAATTG 1 cut(s) 915
MvaI CCWGG 3 cut(s) 486, 1296, 2010
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 6 cut(s) 326, 440, 917, 1296, 1665, 2054
NcoI CCATGG 1 cut(s) 387
NdeII GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
NlaIV GGNNCC 7 cut(s) 52, 319, 362, 694, 1516, 1734, 2273
NmuCI GTSAC 2 cut(s) 233, 1065
NsiI ATGCAT 2 cut(s) 283, 430
NspI RCATGY 3 cut(s) 453, 1305, 1877
OliI CACNNNNGTG 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 1316
PagI TCATGA 1 cut(s) 2140
PceI AGGCCT 1 cut(s) 1668
PdmI GAANNNNTTC 3 cut(s) 9, 550, 1575
PfeI GAWTC 7 cut(s) 394, 1571, 1793, 1827, 1838, 1868, 1911
PinAI ACCGGT 2 cut(s) 1706, 2268
PkrI GCNGC 9 cut(s) 328, 475, 739, 865, 910, 1000, 1123, 1674, 1805
PleI GAGTC 2 cut(s) 580, 2013
PpsI GAGTC 2 cut(s) 580, 2013
PshAI GACNNNNGTC 1 cut(s) 1783
PsiI TTATAA 1 cut(s) 1751
Psp6I CCWGG 3 cut(s) 484, 1294, 2008
PspFI CCCAGC 2 cut(s) 1355, 1368
PspGI CCWGG 3 cut(s) 484, 1294, 2008
PspN4I GGNNCC 7 cut(s) 52, 319, 362, 694, 1516, 1734, 2273
PspPI GGNCC 4 cut(s) 50, 360, 506, 1178
PstI CTGCAG 1 cut(s) 787
PstNI CAGNNNCTG 2 cut(s) 1409, 1702
PsuI RGATCY 3 cut(s) 69, 646, 2367
RsaI GTAC 2 cut(s) 408, 852
RsaNI GTAC 2 cut(s) 407, 851
RseI CAYNNNNRTG 2 cut(s) 402, 1079
SalI GTCGAC 1 cut(s) 1941
SaqAI TTAA 8 cut(s) 15, 168, 276, 639, 1593, 2069, 2330, 2442
SatI GCNGC 9 cut(s) 327, 474, 738, 864, 909, 999, 1122, 1673, 1804
Sau3AI GATC 6 cut(s) 69, 118, 646, 1614, 1678, 2367
Sau96I GGNCC 4 cut(s) 50, 360, 506, 1178
SchI GAGTC 2 cut(s) 581, 2014
ScrFI CCNGG 3 cut(s) 486, 1296, 2010
SduI GDGCHC 2 cut(s) 2140, 2328
SfaNI GCATC 7 cut(s) 238, 290, 535, 724, 850, 1044, 1438
SfcI CTRYAG 2 cut(s) 783, 1788
Sfr274I CTCGAG 1 cut(s) 1316
SinI GGWCC 3 cut(s) 50, 360, 506
SlaI CTCGAG 1 cut(s) 1316
SmiMI CAYNNNNRTG 2 cut(s) 402, 1079
SmlI CTYRAG 6 cut(s) 14, 435, 1025, 1316, 1592, 1769
SmoI CTYRAG 6 cut(s) 14, 435, 1025, 1316, 1592, 1769
SpeI ACTAGT 1 cut(s) 2305
SseBI AGGCCT 1 cut(s) 1668
SsiI CCGC 4 cut(s) 73, 245, 715, 2259
SspMI CTAG 5 cut(s) 644, 1548, 1908, 1968, 2306
StuI AGGCCT 1 cut(s) 1668
StyD4I CCNGG 3 cut(s) 484, 1294, 2008
StyI CCWWGG 1 cut(s) 387
TaiI ACGT 2 cut(s) 1067, 1648
TaqI TCGA 6 cut(s) 253, 927, 1317, 1681, 1764, 1942
TatI WGTACW 1 cut(s) 406
TfiI GAWTC 7 cut(s) 394, 1571, 1793, 1827, 1838, 1868, 1911
Tru1I TTAA 8 cut(s) 15, 168, 276, 639, 1593, 2069, 2330, 2442
Tru9I TTAA 8 cut(s) 15, 168, 276, 639, 1593, 2069, 2330, 2442
TscAI CASTG 6 cut(s) 238, 409, 462, 907, 1150, 1531
TseFI GTSAC 2 cut(s) 233, 1065
TseI GCWGC 9 cut(s) 326, 473, 737, 863, 908, 998, 1121, 1672, 1803
Tsp45I GTSAC 2 cut(s) 233, 1065
TspDTI ATGAA 9 cut(s) 677, 972, 1459, 1470, 1830, 2067, 2157, 2310, 2334
TspGWI ACGGA 4 cut(s) 345, 868, 995, 1288
TspRI CASTG 6 cut(s) 238, 409, 462, 907, 1150, 1531
Vha464I CTTAAG 2 cut(s) 14, 1592
VpaK11BI GGWCC 3 cut(s) 50, 360, 506
XapI RAATTY 6 cut(s) 20, 83, 499, 1263, 1457, 1713
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 3 cut(s) 453, 1305, 1877
XhoI CTCGAG 1 cut(s) 1316
XmiI GTMKAC 4 cut(s) 111, 383, 1608, 1942
XmnI GAANNNNTTC 3 cut(s) 9, 550, 1575
XspI CTAG 5 cut(s) 644, 1548, 1908, 1968, 2306
Zsp2I ATGCAT 2 cut(s) 283, 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.