Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005454

Physical Location & Seq

Genomic Coordinates Reverse (-)

35401467 .. 35409700

Gene Structure 8234 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1221200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGAGGCGAGCAAGGAAGCGTTTCTGAGAGAGTTTGGAGAGCACTACGGATACCCCAACGGACCCAAAACCATCGACGAAATCCGAGCTACCGAATTCAAACGATTAGATGGACTTGTGTACTTGGATCATGCTGGTGCAACTATGTATTCTGAGTTGCAGATGGAAGCAATCTTCAAAGATTTCACTTCCAATGCTTATGGAAACCCACATAGCCAAAGTGATACCAGTTCTGCTACGAGTGACATCGTAAGGGAAGCCCGGCAACAGGTCCTGGACTATTGTAAAGCATCCCCAAAAGATTACAGCTGCATTTTCACTTCTGGGGCTACAGCGGCGCTGAAACTGGTAGGGGAGGCCTTTCCATGGAGCTGTCAGAGCTGTTTTGCTTACACGATGGAGAATCACAATACTTCCAAGTTTGTGCTTAATGTTTGGCATAGTTACACATCAGTTTTGTCAGATGATTTTATTATCCCTGAAATCTTTTTGAAATCAAGTCTGTTCCTTTCATATGCACTGGGTCAAGGAGCTGCAGCTTTTGCAATAGATGTTGAAGAGACTGCGCATCATGGAGTATCTAATGGTACTGTAGCATCCACAAAGGCATTGCAGCACCCCATACAAAGGAGAAGTGAAGCTAGATCCCTGGAGGGAGAACCAACAGGTGATGCATATAACTTATTTGCCTTCCCCTCAGAGTGCAATTTCTCAGGGTTGAGATTTAATCTTGATTTGGTGAAGACAATTAAAGAAGACCCAACAAGAATTCTGGATGGCTCCCCATTTTGCAATGGCCGCTGGATGGTCTTGATTGATGCTGCAAAAGGATCTGCCACAGAACCACCGGATTTATCACAATATCCGGCAGATTTTGTTGTTATGTCTTTCTACAAGCTATTTGGTTATCCAACTGGACTTGGAGCGCTCATTGCTCGAAAGGATGCTTCCAGATTACTAAAGAAGACATACTTCAGTGGAGGCACAGTCGCAGCCTCAATTGCTGATATTGACTTCGTTAGAAGAAGAAAAGGTGTGGAAGAGCTGTTTGAGGACGGTACCATTTCATTCCTGAGCATAGCATCTATTCACCATGGGTTCAAAATTTTCAATTCCCTAACTGAATCTGCCATATCTCGGCATACAGCATCACTTGCAACTTATGTGAGGAAGAAGCTCTTGGCCTTGAGGCATGAAAATGGAGCCAGTGTTTGCTCACTCTATGGAACTTCAAAGGCATCATTTCATGGATTTGGCCCTACAGTCACTTTCAACTTGAAAAGGTTGGATGGATCTTGGTGTGGATACCGTGAAGTGGAAAAACTGGCATCTTTATCTGGAATTCAGTTACGGACAGGATGCTTTTGCAATCCCGGTGCATGTGCAAAATATCTTGGCTTGTCTCACTCAGATCTTCTTTCAAATATTGAGGCTGGCCATGTTTGCTGGGATGATAATGACATAATTCATGGCAAACCTACTGGAGCTGTGAGAGTATCCTTTGGTTATATGTCAACGTTTGAAGATGCAAAGAAATTCGTTGATTTCCTGACAAGTTCGTTTGTGGCATTACCAAATTGGAATGAAAGGGGTTATCAAATAAACCAAGGTCCTGAAAGCAGACTACCAGCTGCTAGTTTGTATCTCAAGTCGATTACTGTCTACCCAATAAAATCATGCGCAGGATTCACCGTGGAAAGTTGGCCTTTGAGCAGTACTGGTCTGCTTCATGATCGAGAATGGGTTCTTACAAGCCCAAGTGGTGAAATTCTTACACAGAAAAAGGTTCCTGACATGTGCTTTATCAGTACTTTTATTGACCTCAATGAGGGAATATTGTTTGTAGAGTCACCACGATGCCAAGCGAGACTGCCAATCAACATTCTGACAGATTCATGCAATGGTGTGCGAGAAGAAATTAAGTTAAATGGCCAGAGATATGAAGTACAGTGTTACGACAATGAAGCCAATATTTGGTTCAGCAATGCCATTGGCCGACCATGCACTTTATTACACTGTTATAGTTCGAATCACAACCATTGCTTGAATAAGAGCAAAAGTATGTGCATGGGCAGAGATGCGCAGGGCATATTGAATTTTTCGAATGAAGCTCAGTTCTTATTAATATCTGAGGAAAGCGTATCTGACCTCAACCGCAGAGTAAGCACAAAAGACGTACAAAAGGGTGCTCGTGGAGCTGCAGGTCAAATCGATCCAATGAGGTTTCGGCCCAACCTCGTCTTATCTGGGGGTGAACCATATGTTGAAGATGGATGGAGAAATCTTAAAATTGGGAATAAATACTTCACATCTTTGGGTGGATGTAATCGTTGCCAGATGATCAACATTGTTCATGAAGCTGGACAAGTGCAGAAGTCAAATGAACCTTTGGCTACTCTAGCATCGTATAGGAGAGCGAAGGGAAAGATTTTGTTTGGGATACTTCTGAAATACGAAAGAAGCGAAGTCATAGGTAGGGACGATGATCTGTGGCTCCGAGTGGGACAAGATGTACAGCCGAATATCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006066 GO:0006082 GO:0006464 GO:0006605 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0008265 GO:0008299 GO:0008610 GO:0009000 GO:0009058 GO:0009266 GO:0009408 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009687 GO:0009688 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009743 GO:0009755 GO:0009756 GO:0009987 GO:0010033 GO:0010118 GO:0010182 GO:0015031 GO:0015833 GO:0016053 GO:0016106 GO:0016114 GO:0016740 GO:0016782 GO:0016783 GO:0016829 GO:0016846 GO:0017038 GO:0018315 GO:0019538 GO:0019752 GO:0023052 GO:0032787 GO:0032870 GO:0033036 GO:0033365 GO:0034613 GO:0036211 GO:0042040 GO:0042221 GO:0042742 GO:0042886 GO:0043170 GO:0043207 GO:0043288 GO:0043289 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0045036 GO:0045037 GO:0045184 GO:0046165 GO:0046394 GO:0046907 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0065002 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071322 GO:0071365 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0072330 GO:0072594 GO:0072596 GO:0072598 GO:0098542 GO:1901564 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00790 map00790

Pfam Domains

Protein Families

Aminotran_5 MOSC MOSC_N

Protein Analysis

844

Amino Acids

93.44

Weight (kDa)

6.51

Isoelectric Point (pI)

39.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aminotran_5	PF00266	40 - 122	8.7e-08	Aminotransferase class-V
Aminotran_5	PF00266	269 - 513	6.8e-18	Aminotransferase class-V
MOSC_N	PF03476	548 - 666	1.2e-18	MOSC N-terminal beta barrel domain
MOSC	PF03473	698 - 829	2.5e-21	MOSC domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005454)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16540 AT1G16540 AT1G16540
fragaria_vesca	FvH4_3g15600 FvH4_3g15600 FvH4_3g15600 FvH4_3g15600
malus_domestica	MD05G1221200.v1.1
prunus_persica	Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1 Prupe.4G138400_v2.0.a1
pyrus_communis	pycom05g20450
rosa_chinensis	RchiOBHm_Chr5g0026221
rosa_laevigata	RLG00000032914
rosa_multiflora	Rmu_sc0000632.1_g000002
rosa_roxburghii	Rroxscaffold_1G00053090
rosa_rugosa	Rorug05G0091600
rosa_samantha	Rh5AG184600 Rh5BG181500 Rh5CG200700 Rh5DG183200
rosa_wichuraiana	Rw5G016740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	3 cut(s)	567, 1681, 2082
Acc36I	ACCTGC	1 cut(s)	2192
Acc65I	GGTACC	1 cut(s)	1058
AccB1I	GGYRCC	1 cut(s)	1058
AccB7I	CCANNNNNTGG	1 cut(s)	1974
AccI	GTMKAC	1 cut(s)	1662
AciI	CCGC	3 cut(s)	335, 799, 2155
AclI	AACGTT	1 cut(s)	1517
AclWI	GGATC	5 cut(s)	135, 639, 838, 1300, 2207
AcoI	YGGCCR	4 cut(s)	796, 1435, 1930, 1993
AcsI	RAATTY	7 cut(s)	95, 768, 1104, 1341, 1535, 1767, 2095
AcuI	CTGAAG	1 cut(s)	958
AfaI	GTAC	8 cut(s)	122, 589, 1060, 1717, 1810, 1947, 2178, 2514
AfeI	AGCGCT	1 cut(s)	927
AfiI	CCNNNNNNNGG	8 cut(s)	268, 366, 627, 805, 1137, 1315, 1828, 1974
AflIII	ACRYGT	1 cut(s)	1794
AjnI	CCWGG	2 cut(s)	273, 648
AjuI	GAANNNNNNNTTGG	4 cut(s)	1163, 1195, 1961, 1993
Alw21I	GWGCWC	2 cut(s)	45, 2191
Alw26I	GTCTC	3 cut(s)	554, 1407, 1861
AlwI	GGATC	5 cut(s)	135, 639, 838, 1300, 2207
AlwNI	CAGNNNCTG	1 cut(s)	274
Aor51HI	AGCGCT	1 cut(s)	927
AoxI	GGCC	9 cut(s)	357, 796, 1182, 1255, 1435, 1703, 1930, 1993, 2228
ApeKI	GCWGC	8 cut(s)	309, 533, 536, 613, 821, 992, 1631, 2198
ApoI	RAATTY	7 cut(s)	95, 768, 1104, 1341, 1535, 1767, 2095
AseI	ATTAAT	1 cut(s)	2123
Asp700I	GAANNNNTTC	2 cut(s)	21, 1743
Asp718I	GGTACC	1 cut(s)	1058
AspLEI	GCGC	5 cut(s)	340, 568, 928, 1682, 2083
AspS9I	GGNCC	5 cut(s)	62, 271, 1256, 1610, 2229
AsuC2I	CCSGG	2 cut(s)	262, 1374
AsuHPI	GGTGA	7 cut(s)	680, 751, 1082, 1681, 1775, 1842, 2264
AsuII	TTCGAA	2 cut(s)	2027, 2102
AvaII	GGWCC	3 cut(s)	62, 271, 1610
BalI	TGGCCA	2 cut(s)	1437, 1932
BanI	GGYRCC	1 cut(s)	1058
BauI	CACGAG	1 cut(s)	2190
BbsI	GAAGAC	3 cut(s)	749, 762, 971
Bbv12I	GWGCWC	2 cut(s)	45, 2191
BbvI	GCAGC	8 cut(s)	296, 520, 548, 625, 808, 1004, 1618, 2185
BccI	CCATC	9 cut(s)	80, 104, 157, 391, 770, 799, 1283, 2264, 2268
BciT130I	CCWGG	2 cut(s)	275, 650
BciVI	GTATCC	4 cut(s)	44, 1298, 1507, 2433
BclI	TGATCA	1 cut(s)	2340
BcnI	CCSGG	2 cut(s)	262, 1374
BcoDI	GTCTC	3 cut(s)	554, 1407, 1861
BfaI	CTAG	3 cut(s)	642, 1635, 2399
BfmI	CTRYAG	5 cut(s)	330, 534, 591, 1260, 2199
BfoI	RGCGCY	2 cut(s)	341, 929
BfuAI	ACCTGC	1 cut(s)	2192
BfuI	GTATCC	4 cut(s)	44, 1298, 1507, 2433
BglII	AGATCT	1 cut(s)	1411
BmcAI	AGTACT	2 cut(s)	1717, 1810
Bme1390I	CCNGG	4 cut(s)	262, 275, 650, 1374
Bme18I	GGWCC	3 cut(s)	62, 271, 1610
BmgT120I	GGNCC	5 cut(s)	62, 271, 1256, 1610, 2229
BmiI	GGNNCC	6 cut(s)	64, 781, 1060, 1204, 1788, 2495
BmrFI	CCNGG	4 cut(s)	262, 275, 650, 1374
BmrI	ACTGGG	1 cut(s)	530
BmuI	ACTGGG	1 cut(s)	530
BpiI	GAAGAC	3 cut(s)	749, 762, 971
BpmI	CTGGAG	2 cut(s)	671, 1503
Bpu10I	CCTNAGC	1 cut(s)	1073
Bpu14I	TTCGAA	2 cut(s)	2027, 2102
BpuEI	CTTGAG	2 cut(s)	1207, 1631
BpuMI	CCSGG	2 cut(s)	262, 1374
Bsa29I	ATCGAT	1 cut(s)	2211
BsaJI	CCNNGG	5 cut(s)	365, 648, 1093, 1606, 1692
BsaWI	WCCGGW	1 cut(s)	847
BsaXI	ACNNNNNCTCC	4 cut(s)	972, 1002, 1194, 1224
Bsc4I	CCNNNNNNNGG	8 cut(s)	268, 366, 627, 805, 1137, 1315, 1828, 1974
Bse1I	ACTGG	8 cut(s)	228, 351, 525, 919, 1206, 1329, 1486, 1723
Bse3DI	GCAATG	6 cut(s)	608, 799, 930, 1906, 1990, 2038
BseBI	CCWGG	2 cut(s)	275, 650
BseCI	ATCGAT	1 cut(s)	2211
BseDI	CCNNGG	5 cut(s)	365, 648, 1093, 1606, 1692
BseLI	CCNNNNNNNGG	8 cut(s)	268, 366, 627, 805, 1137, 1315, 1828, 1974
BseMI	GCAATG	6 cut(s)	608, 799, 930, 1906, 1990, 2038
BseMII	CTCAG	8 cut(s)	17, 144, 711, 726, 1064, 1422, 2121, 2126
BseNI	ACTGG	8 cut(s)	228, 351, 525, 919, 1206, 1329, 1486, 1723
BseXI	GCAGC	8 cut(s)	296, 520, 548, 625, 808, 1004, 1618, 2185
BseYI	CCCAGC	1 cut(s)	1446
BsgI	GTGCAG	1 cut(s)	2390
BshFI	GGCC	9 cut(s)	359, 798, 1184, 1257, 1437, 1705, 1932, 1995, 2230
BshNI	GGYRCC	1 cut(s)	1058
BshVI	ATCGAT	1 cut(s)	2211
BsiHKAI	GWGCWC	2 cut(s)	45, 2191
BsiSI	CCGG	4 cut(s)	262, 848, 866, 1374
BslFI	GGGAC	2 cut(s)	2492, 2517
BslI	CCNNNNNNNGG	8 cut(s)	268, 366, 627, 805, 1137, 1315, 1828, 1974
BsmAI	GTCTC	3 cut(s)	554, 1407, 1861
BsmFI	GGGAC	2 cut(s)	2492, 2517
BsnI	GGCC	9 cut(s)	359, 798, 1184, 1257, 1437, 1705, 1932, 1995, 2230
Bsp119I	TTCGAA	2 cut(s)	2027, 2102
Bsp1286I	GDGCHC	2 cut(s)	45, 2191
Bsp1407I	TGTACA	1 cut(s)	2512
Bsp143I	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
Bsp19I	CCATGG	2 cut(s)	365, 1093
BspACI	CCGC	3 cut(s)	335, 799, 2155
BspANI	GGCC	9 cut(s)	359, 798, 1184, 1257, 1437, 1705, 1932, 1995, 2230
BspCNI	CTCAG	8 cut(s)	18, 145, 710, 725, 1065, 1421, 2122, 2125
BspDI	ATCGAT	1 cut(s)	2211
BspHI	TCATGA	2 cut(s)	1729, 2353
BspLI	GGNNCC	6 cut(s)	64, 781, 1060, 1204, 1788, 2495
BspMAI	CTGCAG	2 cut(s)	538, 2203
BspMI	ACCTGC	1 cut(s)	2192
BspPI	GGATC	5 cut(s)	135, 639, 838, 1300, 2207
BspQI	GCTCTTC	1 cut(s)	1035
BspT104I	TTCGAA	2 cut(s)	2027, 2102
BspT107I	GGYRCC	1 cut(s)	1058
BsrDI	GCAATG	6 cut(s)	608, 799, 930, 1906, 1990, 2038
BsrGI	TGTACA	1 cut(s)	2512
BsrI	ACTGG	8 cut(s)	228, 351, 525, 919, 1206, 1329, 1486, 1723
BssECI	CCNNGG	5 cut(s)	365, 648, 1093, 1606, 1692
BssMI	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
BssSI	CACGAG	1 cut(s)	2190
BssT1I	CCWWGG	3 cut(s)	365, 1093, 1606
Bst2BI	CACGAG	1 cut(s)	2190
Bst2UI	CCWGG	2 cut(s)	275, 650
Bst4CI	ACNGT	9 cut(s)	592, 988, 1058, 1264, 1310, 1660, 1693, 1950, 2018
Bst6I	CTCTTC	2 cut(s)	552, 1035
BstAPI	GCANNNNNTGC	2 cut(s)	542, 1154
BstAUI	TGTACA	1 cut(s)	2512
BstBI	TTCGAA	2 cut(s)	2027, 2102
BstC8I	GCNNGC	2 cut(s)	10, 1435
BstDEI	CTNAG	8 cut(s)	26, 153, 697, 712, 1073, 1408, 2112, 2130
BstDSI	CCRYGG	3 cut(s)	365, 1093, 1692
BstENI	CCTNNNNNAGG	1 cut(s)	1826
BstH2I	RGCGCY	2 cut(s)	341, 929
BstHHI	GCGC	5 cut(s)	340, 568, 928, 1682, 2083
BstKTI	GATC	9 cut(s)	130, 647, 833, 1295, 1414, 1735, 2215, 2343, 2488
BstMAI	GTCTC	3 cut(s)	554, 1407, 1861
BstMBI	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
BstNI	CCWGG	2 cut(s)	275, 650
BstNSI	RCATGY	2 cut(s)	1383, 1798
BstSCI	CCNGG	4 cut(s)	260, 273, 648, 1372
BstSFI	CTRYAG	5 cut(s)	330, 534, 591, 1260, 2199
BstV1I	GCAGC	8 cut(s)	296, 520, 548, 625, 808, 1004, 1618, 2185
BstV2I	GAAGAC	3 cut(s)	749, 762, 971
BstX2I	RGATCY	4 cut(s)	644, 830, 1292, 1411
BstYI	RGATCY	4 cut(s)	644, 830, 1292, 1411
Bsu15I	ATCGAT	1 cut(s)	2211
BsuI	GTATCC	4 cut(s)	44, 1298, 1507, 2433
BsuRI	GGCC	9 cut(s)	359, 798, 1184, 1257, 1437, 1705, 1932, 1995, 2230
BsuTUI	ATCGAT	1 cut(s)	2211
BtgI	CCRYGG	3 cut(s)	365, 1093, 1692
BtsIMutI	CAGTG	5 cut(s)	518, 982, 1213, 1955, 2014
BveI	ACCTGC	1 cut(s)	2192
Cac8I	GCNNGC	2 cut(s)	10, 1435
CaiI	CAGNNNCTG	1 cut(s)	274
CciI	TCATGA	2 cut(s)	1729, 2353
CfoI	GCGC	5 cut(s)	340, 568, 928, 1682, 2083
Cfr13I	GGNCC	5 cut(s)	62, 271, 1256, 1610, 2229
ClaI	ATCGAT	1 cut(s)	2211
Csp6I	GTAC	8 cut(s)	121, 588, 1059, 1716, 1809, 1946, 2177, 2513
CviQI	GTAC	8 cut(s)	121, 588, 1059, 1716, 1809, 1946, 2177, 2513
DdeI	CTNAG	8 cut(s)	26, 153, 697, 712, 1073, 1408, 2112, 2130
DpnI	GATC	9 cut(s)	129, 646, 832, 1294, 1413, 1734, 2214, 2342, 2487
DpnII	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
EaeI	YGGCCR	4 cut(s)	796, 1435, 1930, 1993
Eam1104I	CTCTTC	2 cut(s)	552, 1035
EarI	CTCTTC	2 cut(s)	552, 1035
Eco130I	CCWWGG	3 cut(s)	365, 1093, 1606
Eco147I	AGGCCT	1 cut(s)	359
Eco47I	GGWCC	3 cut(s)	62, 271, 1610
Eco47III	AGCGCT	1 cut(s)	927
Eco57I	CTGAAG	1 cut(s)	958
EcoNI	CCTNNNNNAGG	1 cut(s)	1826
EcoO109I	RGGNCCY	2 cut(s)	271, 1610
EcoRI	GAATTC	3 cut(s)	95, 768, 1341
EcoRII	CCWGG	2 cut(s)	273, 648
EcoT14I	CCWWGG	3 cut(s)	365, 1093, 1606
EcoT22I	ATGCAT	1 cut(s)	676
ErhI	CCWWGG	3 cut(s)	365, 1093, 1606
FalI	AAGNNNNNCTT	6 cut(s)	956, 988, 1163, 1195, 1690, 1722
FaqI	GGGAC	2 cut(s)	2492, 2517
FauNDI	CATATG	2 cut(s)	514, 2260
FbaI	TGATCA	1 cut(s)	2340
FblI	GTMKAC	1 cut(s)	1662
FspBI	CTAG	3 cut(s)	642, 1635, 2399
FspI	TGCGCA	3 cut(s)	567, 1681, 2082
GlaI	GCGC	5 cut(s)	339, 567, 927, 1681, 2082
GsaI	CCCAGC	1 cut(s)	1450
GsuI	CTGGAG	2 cut(s)	671, 1503
HaeII	RGCGCY	2 cut(s)	341, 929
HaeIII	GGCC	9 cut(s)	359, 798, 1184, 1257, 1437, 1705, 1932, 1995, 2230
HapII	CCGG	4 cut(s)	262, 848, 866, 1374
HhaI	GCGC	5 cut(s)	340, 568, 928, 1682, 2083
Hin6I	GCGC	5 cut(s)	338, 566, 926, 1680, 2081
HinP1I	GCGC	5 cut(s)	338, 566, 926, 1680, 2081
HincII	GTYRAC	1 cut(s)	1515
HindII	GTYRAC	1 cut(s)	1515
HinfI	GANTC	6 cut(s)	403, 1124, 1686, 1847, 1892, 2029
HpaII	CCGG	4 cut(s)	262, 848, 866, 1374
HphI	GGTGA	7 cut(s)	680, 751, 1082, 1681, 1775, 1842, 2264
Hpy166II	GTNNAC	4 cut(s)	121, 1515, 1663, 2255
Hpy8I	GTNNAC	4 cut(s)	121, 1515, 1663, 2255
Hpy99I	CGWCG	1 cut(s)	80
HpyAV	CCTTC	2 cut(s)	700, 2413
HpyCH4III	ACNGT	9 cut(s)	592, 988, 1058, 1264, 1310, 1660, 1693, 1950, 2018
HpyCH4IV	ACGT	2 cut(s)	1517, 2175
HpyF3I	CTNAG	8 cut(s)	26, 153, 697, 712, 1073, 1408, 2112, 2130
HpySE526I	ACGT	2 cut(s)	1517, 2175
HspAI	GCGC	5 cut(s)	338, 566, 926, 1680, 2081
KpnI	GGTACC	1 cut(s)	1062
Ksp22I	TGATCA	1 cut(s)	2340
Kzo9I	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
LguI	GCTCTTC	1 cut(s)	1035
LmnI	GCTCC	8 cut(s)	369, 530, 785, 923, 1202, 1484, 2195, 2499
Lsp1109I	GCAGC	8 cut(s)	296, 520, 548, 625, 808, 1004, 1618, 2185
MaeI	CTAG	3 cut(s)	642, 1635, 2399
MaeII	ACGT	2 cut(s)	1517, 2175
MaeIII	GTNAC	6 cut(s)	242, 443, 1264, 1347, 1848, 1952
MalI	GATC	9 cut(s)	129, 646, 832, 1294, 1413, 1734, 2214, 2342, 2487
MboI	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
MfeI	CAATTG	1 cut(s)	999
MflI	RGATCY	4 cut(s)	644, 830, 1292, 1411
MhlI	GDGCHC	2 cut(s)	45, 2191
MlsI	TGGCCA	2 cut(s)	1437, 1932
MluNI	TGGCCA	2 cut(s)	1437, 1932
MlyI	GAGTC	1 cut(s)	1856
MmeI	TCCRAC	2 cut(s)	935, 1266
Mox20I	TGGCCA	2 cut(s)	1437, 1932
Mph1103I	ATGCAT	1 cut(s)	676
MroXI	GAANNNNTTC	2 cut(s)	21, 1743
MscI	TGGCCA	2 cut(s)	1437, 1932
MseI	TTAA	7 cut(s)	429, 726, 750, 1920, 1925, 2123, 2286
MslI	CAYNNNNRTG	3 cut(s)	135, 1197, 1855
Msp20I	TGGCCA	2 cut(s)	1437, 1932
MspA1I	CMGCKG	4 cut(s)	309, 335, 801, 1631
MspI	CCGG	4 cut(s)	262, 848, 866, 1374
MspR9I	CCNGG	4 cut(s)	262, 275, 650, 1374
MunI	CAATTG	1 cut(s)	999
MvaI	CCWGG	2 cut(s)	275, 650
NciI	CCSGG	2 cut(s)	262, 1374
NcoI	CCATGG	2 cut(s)	365, 1093
NdeI	CATATG	2 cut(s)	514, 2260
NdeII	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
NlaIV	GGNNCC	6 cut(s)	64, 781, 1060, 1204, 1788, 2495
NmeAIII	GCCGAG	1 cut(s)	1117
NmuCI	GTSAC	3 cut(s)	242, 1264, 1848
NsbI	TGCGCA	3 cut(s)	567, 1681, 2082
NsiI	ATGCAT	1 cut(s)	676
NspI	RCATGY	2 cut(s)	1383, 1798
NspV	TTCGAA	2 cut(s)	2027, 2102
PagI	TCATGA	2 cut(s)	1729, 2353
PceI	AGGCCT	1 cut(s)	359
PciI	ACATGT	1 cut(s)	1794
PciSI	GCTCTTC	1 cut(s)	1035
PcsI	WCGNNNNNNNCGW	1 cut(s)	2460
PdmI	GAANNNNTTC	2 cut(s)	21, 1743
PfeI	GAWTC	5 cut(s)	403, 1124, 1686, 1892, 2029
PflMI	CCANNNNNTGG	1 cut(s)	1974
PfoI	TCCNGGA	1 cut(s)	273
PleI	GAGTC	1 cut(s)	1855
PpsI	GAGTC	1 cut(s)	1855
PpuMI	RGGWCCY	2 cut(s)	271, 1610
PscI	ACATGT	1 cut(s)	1794
PshBI	ATTAAT	1 cut(s)	2123
Psp1406I	AACGTT	1 cut(s)	1517
Psp5II	RGGWCCY	2 cut(s)	271, 1610
Psp6I	CCWGG	2 cut(s)	273, 648
PspFI	CCCAGC	1 cut(s)	1446
PspGI	CCWGG	2 cut(s)	273, 648
PspN4I	GGNNCC	6 cut(s)	64, 781, 1060, 1204, 1788, 2495
PspPI	GGNCC	5 cut(s)	62, 271, 1256, 1610, 2229
PspPPI	RGGWCCY	2 cut(s)	271, 1610
PstI	CTGCAG	2 cut(s)	538, 2203
PstNI	CAGNNNCTG	1 cut(s)	274
PsuI	RGATCY	4 cut(s)	644, 830, 1292, 1411
PvuII	CAGCTG	2 cut(s)	309, 1631
RsaI	GTAC	8 cut(s)	122, 589, 1060, 1717, 1810, 1947, 2178, 2514
RsaNI	GTAC	8 cut(s)	121, 588, 1059, 1716, 1809, 1946, 2177, 2513
RseI	CAYNNNNRTG	3 cut(s)	135, 1197, 1855
SapI	GCTCTTC	1 cut(s)	1035
SaqAI	TTAA	7 cut(s)	429, 726, 750, 1920, 1925, 2123, 2286
Sau3AI	GATC	9 cut(s)	127, 644, 830, 1292, 1411, 1732, 2212, 2340, 2485
Sau96I	GGNCC	5 cut(s)	62, 271, 1256, 1610, 2229
ScaI	AGTACT	2 cut(s)	1717, 1810
SchI	GAGTC	1 cut(s)	1856
ScrFI	CCNGG	4 cut(s)	262, 275, 650, 1374
SduI	GDGCHC	2 cut(s)	45, 2191
SfcI	CTRYAG	5 cut(s)	330, 534, 591, 1260, 2199
SfuI	TTCGAA	2 cut(s)	2027, 2102
SinI	GGWCC	3 cut(s)	62, 271, 1610
SmiMI	CAYNNNNRTG	3 cut(s)	135, 1197, 1855
SmlI	CTYRAG	2 cut(s)	1186, 1646
SmoI	CTYRAG	2 cut(s)	1186, 1646
SseBI	AGGCCT	1 cut(s)	359
SsiI	CCGC	3 cut(s)	335, 799, 2155
SspI	AATATT	3 cut(s)	1426, 1836, 1972
SspMI	CTAG	3 cut(s)	642, 1635, 2399
StuI	AGGCCT	1 cut(s)	359
StyD4I	CCNGG	4 cut(s)	260, 273, 648, 1372
StyI	CCWWGG	3 cut(s)	365, 1093, 1606
TaaI	ACNGT	9 cut(s)	592, 988, 1058, 1264, 1310, 1660, 1693, 1950, 2018
TaiI	ACGT	2 cut(s)	1520, 2178
TaqI	TCGA	7 cut(s)	75, 937, 1652, 1735, 2027, 2102, 2211
TatI	WGTACW	5 cut(s)	120, 1715, 1808, 1945, 2512
TauI	GCSGC	2 cut(s)	338, 801
TfiI	GAWTC	5 cut(s)	403, 1124, 1686, 1892, 2029
Tru1I	TTAA	7 cut(s)	429, 726, 750, 1920, 1925, 2123, 2286
Tru9I	TTAA	7 cut(s)	429, 726, 750, 1920, 1925, 2123, 2286
TscAI	CASTG	5 cut(s)	525, 982, 1213, 1955, 2021
TseFI	GTSAC	3 cut(s)	242, 1264, 1848
TseI	GCWGC	8 cut(s)	309, 533, 536, 613, 821, 992, 1631, 2198
Tsp45I	GTSAC	3 cut(s)	242, 1264, 1848
TspGWI	ACGGA	3 cut(s)	63, 75, 1366
TspRI	CASTG	5 cut(s)	525, 982, 1213, 1955, 2021
Van91I	CCANNNNNTGG	1 cut(s)	1974
VpaK11BI	GGWCC	3 cut(s)	62, 271, 1610
VspI	ATTAAT	1 cut(s)	2123
XagI	CCTNNNNNAGG	1 cut(s)	1826
XapI	RAATTY	7 cut(s)	95, 768, 1104, 1341, 1535, 1767, 2095
XceI	RCATGY	2 cut(s)	1383, 1798
XmiI	GTMKAC	1 cut(s)	1662
XmnI	GAANNNNTTC	2 cut(s)	21, 1743
XspI	CTAG	3 cut(s)	642, 1635, 2399
ZrmI	AGTACT	2 cut(s)	1717, 1810
Zsp2I	ATGCAT	1 cut(s)	676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.