AT1G17665

3hydroxyisobutyrate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016707
Physical Location & Seq
Reverse (-)
6072153 .. 6074525
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G17665.1

Sequence Viewer

Length: 1017 bp
ATGGCGGCTCCGCATTTCACACAACTCAAAATTACACTAAACCCTCTCATGTATCCCTTTCTCGTCTTATCTCTACTAACTCTCGCCCTCTTCTCATTCGTCTCCGCCATCTTCTTTCTCCTCAAAGCTTCCCGCAGCAGAGCTGCTTTGTACAGCCAGAAACTCTTATCCGAATCCGAAACCAAACTCCAACCAGAATCGTCTCTATCGGAGATTTCCGACGAAGCCCAGTACCAAACCCATGAAAATGAACCGACCCATTTGACGAATTCGCGACTCTATGAGTTACTGCTCTCCGATAAGAAGGAGGATGATTCGGATTGGGAAGGAGATCATGTGAAAAAGAAGAAGAAGAAGAAGAAGAATCGAGGTAAGAAGAAGAAATCAGACATAAGAGGAGATGAATCCGGCGGCGAAAAGCAGCTCGGTGAGGGAGAAGATGGGCTTGTTTTGAATCCGAGGACAGACTCGATTTCGATATCGGAAAACAAACCGGAGTTTGTTTGTTTATATCCTTTTACATCGACGAGCAGTGCTACGCAGAGGAAGATTAAGCAGCAATACGATCAGCTTGTTAAATGCAATAATGCCAAAGGATTGACACTAGCTCAGGTTGGGGAGTTTGCTAATTGTTTGATAGAAGCCAAAAATGAACTACAACACAAGTCAGAAGTAATCAAGCGCAAGTTTTCAATAACAAAAGCCCTTCTCTTTAAGGCTGATAGATCTTCCTTTGACCGACTTCGTCAACAGATCTATAAGCTGGAGATGGAACAAAAAAGAGTAGAAGAAGATGCACTTGTATATAATTGGCTCCAGCAACAGCTTAAACTCTCACCTGCATACAAAAAGGTTCTTGAAATAAGCGCTTCCATGGAACTCAAAGACAAATCGAGCACAGAGTTAGACAATCCAGATGATGAATTTTCAGACATTTCCTTCGAAGAGCTATTGGAACAGGAAAAGAAAGACTCGTTTTGGCATAAAAATGGAAGATTGCGAACTTGCAGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

38.92

Weight (kDa)

8.41

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17665
fragaria_vesca FvH4_6g33270
malus_domestica MD17G1174400.v1.1
prunus_persica Prupe.3G050100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142971
rosa_laevigata RLG00000020005
rosa_multiflora Rmu_sc0002277.1_g000025
rosa_roxburghii Rroxscaffold_2G00102170
rosa_rugosa Rorug02G0377400
rosa_samantha Rh2AG430700 Rh2BG439600 Rh2CG416300 Rh2DG449600
rosa_wichuraiana Rw2G035170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 847
Acc36I ACCTGC 1 cut(s) 847
AccII CGCG 1 cut(s) 274
AciI CCGC 5 cut(s) 5, 11, 105, 133, 411
AcsI RAATTY 2 cut(s) 268, 923
AfaI GTAC 2 cut(s) 152, 233
AfeI AGCGCT 1 cut(s) 868
AgsI TTSAA 3 cut(s) 454, 693, 860
AluBI AGCT 8 cut(s) 128, 143, 424, 571, 608, 763, 826, 949
AluI AGCT 8 cut(s) 128, 143, 424, 571, 608, 763, 826, 949
Alw21I GWGCWC 1 cut(s) 899
Alw26I GTCTC 2 cut(s) 106, 207
Aor51HI AGCGCT 1 cut(s) 868
ApeKI GCWGC 4 cut(s) 135, 143, 421, 556
ApoI RAATTY 2 cut(s) 268, 923
ArsI GACNNNNNNTTYG 2 cut(s) 923, 955
AspLEI GCGC 2 cut(s) 684, 869
AsuHPI GGTGA 2 cut(s) 440, 828
AsuII TTCGAA 1 cut(s) 942
Bbv12I GWGCWC 1 cut(s) 899
BbvI GCAGC 4 cut(s) 130, 147, 433, 568
BccI CCATC 3 cut(s) 116, 434, 763
BciVI GTATCC 1 cut(s) 63
BcoDI GTCTC 2 cut(s) 106, 207
BfaI CTAG 1 cut(s) 605
BfoI RGCGCY 1 cut(s) 870
BfuAI ACCTGC 1 cut(s) 847
BfuI GTATCC 1 cut(s) 63
BglII AGATCT 2 cut(s) 725, 753
BisI GCNGC 6 cut(s) 6, 136, 144, 412, 422, 557
BlsI GCNGC 6 cut(s) 7, 137, 145, 413, 423, 558
BmiI GGNNCC 2 cut(s) 9, 815
BmrI ACTGGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 784
BmuI ACTGGG 1 cut(s) 223
BpmI CTGGAG 2 cut(s) 785, 800
Bpu10I CCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 458, 873
BsaWI WCCGGW 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bse1I ACTGG 1 cut(s) 229
BseDI CCNNGG 2 cut(s) 458, 873
BseGI GGATG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 1 cut(s) 229
BseRI GAGGAG 2 cut(s) 110, 411
BseXI GCAGC 4 cut(s) 130, 147, 433, 568
Bsh1236I CGCG 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 899
BsiSI CCGG 2 cut(s) 408, 494
BsmAI GTCTC 2 cut(s) 106, 207
BsmBI CGTCTC 2 cut(s) 106, 207
Bsp119I TTCGAA 1 cut(s) 942
Bsp1286I GDGCHC 1 cut(s) 899
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 4 cut(s) 331, 565, 725, 753
Bsp19I CCATGG 1 cut(s) 873
Bsp68I TCGCGA 1 cut(s) 274
BspACI CCGC 5 cut(s) 5, 11, 105, 133, 411
BspCNI CTCAG 1 cut(s) 622
BspFNI CGCG 1 cut(s) 274
BspLI GGNNCC 2 cut(s) 9, 815
BspMI ACCTGC 1 cut(s) 847
BspQI GCTCTTC 1 cut(s) 939
BspT104I TTCGAA 1 cut(s) 942
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 458, 873
BssMI GATC 4 cut(s) 331, 565, 725, 753
BssT1I CCWWGG 1 cut(s) 873
Bst6I CTCTTC 2 cut(s) 95, 939
BstAUI TGTACA 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 942
BstDEI CTNAG 1 cut(s) 609
BstDSI CCRYGG 1 cut(s) 873
BstF5I GGATG 1 cut(s) 316
BstFNI CGCG 1 cut(s) 274
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 2 cut(s) 684, 869
BstKTI GATC 4 cut(s) 334, 568, 728, 756
BstMAI GTCTC 2 cut(s) 106, 207
BstMBI GATC 4 cut(s) 331, 565, 725, 753
BstUI CGCG 1 cut(s) 274
BstV1I GCAGC 4 cut(s) 130, 147, 433, 568
BstX2I RGATCY 2 cut(s) 725, 753
BstYI RGATCY 2 cut(s) 725, 753
BsuI GTATCC 1 cut(s) 63
BtgI CCRYGG 1 cut(s) 873
BtsCI GGATG 1 cut(s) 316
BtsI GCAGTG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 538
BtuMI TCGCGA 1 cut(s) 274
BveI ACCTGC 1 cut(s) 847
CfoI GCGC 2 cut(s) 684, 869
Csp6I GTAC 2 cut(s) 151, 232
CviAII CATG 5 cut(s) 49, 242, 335, 874, 1014
CviQI GTAC 2 cut(s) 151, 232
DdeI CTNAG 1 cut(s) 609
DpnI GATC 4 cut(s) 333, 567, 727, 755
DpnII GATC 4 cut(s) 331, 565, 725, 753
Eam1104I CTCTTC 2 cut(s) 95, 939
EarI CTCTTC 2 cut(s) 95, 939
EciI GGCGGA 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 873
Eco32I GATATC 1 cut(s) 480
Eco47III AGCGCT 1 cut(s) 868
EcoRI GAATTC 1 cut(s) 268
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 873
ErhI CCWWGG 1 cut(s) 873
Esp3I CGTCTC 2 cut(s) 106, 207
FaeI CATG 5 cut(s) 52, 245, 338, 877, 1017
FalI AAGNNNNNCTT 4 cut(s) 429, 461, 783, 815
FatI CATG 5 cut(s) 48, 241, 334, 873, 1013
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 6 cut(s) 6, 136, 144, 412, 422, 557
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 6 cut(s) 6, 136, 144, 412, 422, 557
FspBI CTAG 1 cut(s) 605
GlaI GCGC 2 cut(s) 683, 868
GluI GCNGC 6 cut(s) 6, 136, 144, 412, 422, 557
GsuI CTGGAG 2 cut(s) 785, 800
HaeII RGCGCY 1 cut(s) 870
HapII CCGG 2 cut(s) 408, 494
HhaI GCGC 2 cut(s) 684, 869
Hin1II CATG 5 cut(s) 52, 245, 338, 877, 1017
Hin6I GCGC 2 cut(s) 682, 867
HinP1I GCGC 2 cut(s) 682, 867
HincII GTYRAC 1 cut(s) 749
HindII GTYRAC 1 cut(s) 749
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 9 cut(s) 173, 197, 276, 314, 364, 404, 454, 467, 971
HpaII CCGG 2 cut(s) 408, 494
HphI GGTGA 2 cut(s) 440, 828
Hpy166II GTNNAC 1 cut(s) 749
Hpy188III TCNNGA 3 cut(s) 273, 857, 914
Hpy8I GTNNAC 1 cut(s) 749
Hpy99I CGWCG 2 cut(s) 224, 529
HpyAV CCTTC 4 cut(s) 298, 320, 716, 949
HpyCH4V TGCA 4 cut(s) 582, 797, 842, 1008
HpyF3I CTNAG 1 cut(s) 609
Hsp92II CATG 5 cut(s) 52, 245, 338, 877, 1017
HspAI GCGC 2 cut(s) 682, 867
Kzo9I GATC 4 cut(s) 331, 565, 725, 753
LguI GCTCTTC 1 cut(s) 939
LmnI GCTCC 2 cut(s) 13, 819
Lsp1109I GCAGC 4 cut(s) 130, 147, 433, 568
LweI GCATC 1 cut(s) 784
MaeI CTAG 1 cut(s) 605
MaeIII GTNAC 1 cut(s) 285
MalI GATC 4 cut(s) 333, 567, 727, 755
MboI GATC 4 cut(s) 331, 565, 725, 753
MflI RGATCY 2 cut(s) 725, 753
MhlI GDGCHC 1 cut(s) 899
MluCI AATT 5 cut(s) 30, 268, 628, 808, 923
MlyI GAGTC 3 cut(s) 270, 461, 965
MmeI TCCRAC 2 cut(s) 214, 243
MnlI CCTC 9 cut(s) 54, 98, 131, 301, 362, 389, 424, 453, 537
MseI TTAA 4 cut(s) 552, 576, 714, 828
MslI CAYNNNNRTG 2 cut(s) 246, 987
MspI CCGG 2 cut(s) 408, 494
MvnI CGCG 1 cut(s) 274
NcoI CCATGG 1 cut(s) 873
NdeII GATC 4 cut(s) 331, 565, 725, 753
NlaIII CATG 5 cut(s) 52, 245, 338, 877, 1017
NlaIV GGNNCC 2 cut(s) 9, 815
NruI TCGCGA 1 cut(s) 274
NspV TTCGAA 1 cut(s) 942
PaqCI CACCTGC 1 cut(s) 847
PciSI GCTCTTC 1 cut(s) 939
PfeI GAWTC 6 cut(s) 173, 197, 314, 364, 404, 454
PflFI GACNNNGTC 1 cut(s) 744
PkrI GCNGC 6 cut(s) 7, 137, 145, 413, 423, 558
PleI GAGTC 3 cut(s) 270, 461, 965
PpsI GAGTC 3 cut(s) 270, 461, 965
PspN4I GGNNCC 2 cut(s) 9, 815
PsuI RGATCY 2 cut(s) 725, 753
PsyI GACNNNGTC 1 cut(s) 744
RruI TCGCGA 1 cut(s) 274
RsaI GTAC 2 cut(s) 152, 233
RsaNI GTAC 2 cut(s) 151, 232
RseI CAYNNNNRTG 2 cut(s) 246, 987
SapI GCTCTTC 1 cut(s) 939
SaqAI TTAA 4 cut(s) 552, 576, 714, 828
SatI GCNGC 6 cut(s) 6, 136, 144, 412, 422, 557
Sau3AI GATC 4 cut(s) 331, 565, 725, 753
SchI GAGTC 3 cut(s) 270, 461, 965
SduI GDGCHC 1 cut(s) 899
SfaNI GCATC 1 cut(s) 784
SfuI TTCGAA 1 cut(s) 942
SmiMI CAYNNNNRTG 2 cut(s) 246, 987
Sse9I AATT 5 cut(s) 30, 268, 628, 808, 923
SsiI CCGC 5 cut(s) 5, 11, 105, 133, 411
SspMI CTAG 1 cut(s) 605
StyI CCWWGG 1 cut(s) 873
TaqI TCGA 6 cut(s) 367, 470, 476, 524, 893, 942
TaqII GACCGA 1 cut(s) 753
TasI AATT 5 cut(s) 30, 268, 628, 808, 923
TatI WGTACW 1 cut(s) 150
TauI GCSGC 2 cut(s) 8, 414
TfiI GAWTC 6 cut(s) 173, 197, 314, 364, 404, 454
Tru1I TTAA 4 cut(s) 552, 576, 714, 828
Tru9I TTAA 4 cut(s) 552, 576, 714, 828
TscAI CASTG 1 cut(s) 538
TseI GCWGC 4 cut(s) 135, 143, 421, 556
TspDTI ATGAA 5 cut(s) 258, 264, 417, 666, 936
TspRI CASTG 1 cut(s) 538
Tth111I GACNNNGTC 1 cut(s) 744
XapI RAATTY 2 cut(s) 268, 923
XspI CTAG 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.