FvH4_6g33270

3hydroxyisobutyrate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016707
Physical Location & Seq
Forward (+)
26344640 .. 26348142
3503 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g33270.t1

Sequence Viewer

Length: 897 bp
ATGTCCCATCTCTGCCTCTTCGTCGTCTTCGTCTTCACTTTCTTCGTCGTAATACCTTTCTTTGTTTTTGTAAAGCTGAAGAGAACAGAGCTCAAGCTGCAGAGACGGAATGACCTGTTTGAACTAAACCTGTTTCTATATCAGACTCTGGCCAAGACCCACCATGTGGACGATGGAAATGACCCGACCCGTTTGGCCCAACAAGGGTCGGGCTCCGAGGACGAGGAGAGGGACGGGTCCGGCGGCGGCATTGAGAAAGTGAAGAAGAAGAAGAGGGCCAAGAAGAAGAATTCATCACAATTGGAATGTGGTGGTGGAAATGAGGATATGAGTGAGAAGGGGAATTCGGGTCCGGTTTCGGGTTCTCGGGTCAAGGCTGATGTGGTGTGTTTCTACCCGTTTACCTCATCCACTAGTGCTACACAGAGGAAGATCAAGAAGCAATATGATGAGCTTATGAAGTGTAATGCATCCAAGAAATTGACACTCTCTCAGGTTGGACAGTTTGCTACTTGCTTAGTTGAGGCTAGAAATGAGCTACAGCACAAGGCTGATGCCATTCAGCGTAAATTTACAATAAGAAAAGCTCTACTAAACAAGGCAGATAGATCTTCTTTTGACCGCCTTCGTAAACAGATATGCAAGCTAGAATTAGAACAGAAGAGACTGGAAGAGGATGCATCTGTCTACAATTGGCTCCAACATCAGCTTAAGCTTTCACCAGCATACAAGAAGATGCTTGAAATTGCCTGCACTGAGTTGAAGACCAAATCCAGAGAGCAAATGGAGAGTAAAGATGTAGATTTTACTGGAATTTCTTTTGAAGATTTCCTGGCACAAGAAAAGAAGGATTCATTCTGGCAGAAAAACATGAAATCAAGATCATGCTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

34.17

Weight (kDa)

9.39

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17665
fragaria_vesca FvH4_6g33270
malus_domestica MD17G1174400.v1.1
prunus_persica Prupe.3G050100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142971
rosa_laevigata RLG00000020005
rosa_multiflora Rmu_sc0002277.1_g000025
rosa_roxburghii Rroxscaffold_2G00102170
rosa_rugosa Rorug02G0377400
rosa_samantha Rh2AG430700 Rh2BG439600 Rh2CG416300 Rh2DG449600
rosa_wichuraiana Rw2G035170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 166
AccI GTMKAC 1 cut(s) 687
AciI CCGC 3 cut(s) 243, 246, 622
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 4 cut(s) 289, 343, 569, 813
AcuI CTGAAG 1 cut(s) 98
AdeI CACNNNGTG 1 cut(s) 166
AfiI CCNNNNNNNGG 3 cut(s) 166, 204, 359
AflII CTTAAG 1 cut(s) 710
AgsI TTSAA 4 cut(s) 122, 743, 763, 824
AhlI ACTAGT 1 cut(s) 413
AjnI CCWGG 1 cut(s) 831
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 2 cut(s) 97, 658
AlwNI CAGNNNCTG 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 366
AoxI GGCC 3 cut(s) 150, 195, 276
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 4 cut(s) 289, 343, 569, 813
AspS9I GGNCC 4 cut(s) 196, 237, 276, 350
AsuHPI GGTGA 1 cut(s) 711
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 2 cut(s) 237, 350
BalI TGGCCA 1 cut(s) 152
BanII GRGCYC 2 cut(s) 93, 215
BbsI GAAGAC 3 cut(s) 19, 25, 770
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 1 cut(s) 84
BccI CCATC 2 cut(s) 15, 167
BciT130I CCWGG 1 cut(s) 833
BcoDI GTCTC 2 cut(s) 97, 658
BcuI ACTAGT 1 cut(s) 413
BfaI CTAG 3 cut(s) 414, 528, 647
BfmI CTRYAG 2 cut(s) 98, 539
BfrI CTTAAG 1 cut(s) 710
BglII AGATCT 1 cut(s) 608
BisI GCNGC 3 cut(s) 98, 244, 247
BlsI GCNGC 3 cut(s) 99, 245, 248
Bme1390I CCNGG 1 cut(s) 833
Bme18I GGWCC 2 cut(s) 237, 350
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 4 cut(s) 196, 237, 276, 350
BmiI GGNNCC 4 cut(s) 214, 238, 351, 698
BmrFI CCNGG 1 cut(s) 833
BmsI GCATC 5 cut(s) 479, 544, 667, 689, 726
BpiI GAAGAC 3 cut(s) 19, 25, 770
BpuEI CTTGAG 2 cut(s) 77, 874
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 204, 359
Bse1I ACTGG 2 cut(s) 672, 814
BseBI CCWGG 1 cut(s) 833
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 3 cut(s) 407, 470, 682
BseLI CCNNNNNNNGG 3 cut(s) 166, 204, 359
BseMII CTCAG 2 cut(s) 506, 747
BseNI ACTGG 2 cut(s) 672, 814
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 84
BsgI GTGCAG 1 cut(s) 736
BshFI GGCC 3 cut(s) 152, 197, 278
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 2 cut(s) 240, 353
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 3 cut(s) 166, 204, 359
BsmAI GTCTC 2 cut(s) 97, 658
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 3 cut(s) 152, 197, 278
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 93, 215
Bsp143I GATC 3 cut(s) 432, 608, 881
BspACI CCGC 3 cut(s) 243, 246, 622
BspANI GGCC 3 cut(s) 152, 197, 278
BspCNI CTCAG 2 cut(s) 505, 748
BspLI GGNNCC 4 cut(s) 214, 238, 351, 698
BspMAI CTGCAG 1 cut(s) 102
BspTI CTTAAG 1 cut(s) 710
BsrI ACTGG 2 cut(s) 672, 814
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 3 cut(s) 432, 608, 881
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 1 cut(s) 504
Bst6I CTCTTC 5 cut(s) 23, 74, 266, 656, 666
BstAFI CTTAAG 1 cut(s) 710
BstC8I GCNNGC 2 cut(s) 644, 751
BstDEI CTNAG 3 cut(s) 492, 517, 756
BstF5I GGATG 3 cut(s) 407, 470, 682
BstKTI GATC 3 cut(s) 435, 611, 884
BstMAI GTCTC 2 cut(s) 97, 658
BstMBI GATC 3 cut(s) 432, 608, 881
BstMWI GCNNNNNNNGC 1 cut(s) 97
BstNI CCWGG 1 cut(s) 833
BstSCI CCNGG 1 cut(s) 831
BstSFI CTRYAG 2 cut(s) 98, 539
BstV1I GCAGC 1 cut(s) 84
BstV2I GAAGAC 3 cut(s) 19, 25, 770
BstX2I RGATCY 1 cut(s) 608
BstYI RGATCY 1 cut(s) 608
BsuRI GGCC 3 cut(s) 152, 197, 278
BtsCI GGATG 3 cut(s) 407, 470, 682
BtsIMutI CAGTG 1 cut(s) 753
Cac8I GCNNGC 2 cut(s) 644, 751
CaiI CAGNNNCTG 1 cut(s) 148
Cfr13I GGNCC 4 cut(s) 196, 237, 276, 350
CviAII CATG 3 cut(s) 164, 871, 885
DdeI CTNAG 3 cut(s) 492, 517, 756
DpnI GATC 3 cut(s) 434, 610, 883
DpnII GATC 3 cut(s) 432, 608, 881
DraIII CACNNNGTG 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 5 cut(s) 23, 74, 266, 656, 666
EarI CTCTTC 5 cut(s) 23, 74, 266, 656, 666
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 2 cut(s) 93, 215
Eco47I GGWCC 2 cut(s) 237, 350
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 91
EcoRI GAATTC 2 cut(s) 289, 343
EcoRII CCWGG 1 cut(s) 831
EcoT22I ATGCAT 2 cut(s) 472, 682
EcoT38I GRGCYC 2 cut(s) 93, 215
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 3 cut(s) 167, 874, 888
FaiI YATR 9 cut(s) 139, 165, 329, 447, 458, 640, 727, 872, 886
FaqI GGGAC 1 cut(s) 245
FatI CATG 3 cut(s) 163, 870, 884
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 3 cut(s) 98, 244, 247
FokI GGATG 3 cut(s) 394, 457, 689
FriOI GRGCYC 2 cut(s) 93, 215
Fsp4HI GCNGC 3 cut(s) 98, 244, 247
FspBI CTAG 3 cut(s) 414, 528, 647
GluI GCNGC 3 cut(s) 98, 244, 247
HaeIII GGCC 3 cut(s) 152, 197, 278
HapII CCGG 2 cut(s) 240, 353
Hin1II CATG 3 cut(s) 167, 874, 888
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 2 cut(s) 145, 851
HpaII CCGG 2 cut(s) 240, 353
HphI GGTGA 1 cut(s) 711
Hpy166II GTNNAC 4 cut(s) 169, 402, 632, 688
Hpy188I TCNGA 2 cut(s) 144, 217
Hpy188III TCNNGA 3 cut(s) 436, 774, 879
Hpy8I GTNNAC 4 cut(s) 169, 402, 632, 688
Hpy99I CGWCG 2 cut(s) 26, 50
HpyAV CCTTC 3 cut(s) 331, 635, 841
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4V TGCA 5 cut(s) 100, 470, 642, 680, 753
HpyF10VI GCNNNNNNNGC 1 cut(s) 97
HpyF3I CTNAG 3 cut(s) 492, 517, 756
Hsp92II CATG 3 cut(s) 167, 874, 888
Kzo9I GATC 3 cut(s) 432, 608, 881
LmnI GCTCC 2 cut(s) 218, 702
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 5 cut(s) 479, 544, 667, 689, 726
MaeI CTAG 3 cut(s) 414, 528, 647
MalI GATC 3 cut(s) 434, 610, 883
MboI GATC 3 cut(s) 432, 608, 881
MfeI CAATTG 2 cut(s) 299, 691
MflI RGATCY 1 cut(s) 608
MhlI GDGCHC 2 cut(s) 93, 215
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 9 cut(s) 289, 299, 343, 479, 569, 650, 691, 744, 813
MluNI TGGCCA 1 cut(s) 152
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 2 cut(s) 478, 724
Mox20I TGGCCA 1 cut(s) 152
Mph1103I ATGCAT 2 cut(s) 472, 682
MscI TGGCCA 1 cut(s) 152
MseI TTAA 1 cut(s) 711
Msp20I TGGCCA 1 cut(s) 152
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 2 cut(s) 240, 353
MspR9I CCNGG 1 cut(s) 833
MunI CAATTG 2 cut(s) 299, 691
MvaI CCWGG 1 cut(s) 833
MwoI GCNNNNNNNGC 1 cut(s) 97
NdeII GATC 3 cut(s) 432, 608, 881
NlaIII CATG 3 cut(s) 167, 874, 888
NlaIV GGNNCC 4 cut(s) 214, 238, 351, 698
NsiI ATGCAT 2 cut(s) 472, 682
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PfeI GAWTC 1 cut(s) 851
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 3 cut(s) 99, 245, 248
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
Psp124BI GAGCTC 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 831
PspGI CCWGG 1 cut(s) 831
PspN4I GGNNCC 4 cut(s) 214, 238, 351, 698
PspPI GGNCC 4 cut(s) 196, 237, 276, 350
PstI CTGCAG 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 148
PsuI RGATCY 1 cut(s) 608
SacI GAGCTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 3 cut(s) 98, 244, 247
Sau3AI GATC 3 cut(s) 432, 608, 881
Sau96I GGNCC 4 cut(s) 196, 237, 276, 350
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 833
SduI GDGCHC 2 cut(s) 93, 215
SfaNI GCATC 5 cut(s) 479, 544, 667, 689, 726
SfcI CTRYAG 2 cut(s) 98, 539
SinI GGWCC 2 cut(s) 237, 350
SmlI CTYRAG 3 cut(s) 92, 710, 889
SmoI CTYRAG 3 cut(s) 92, 710, 889
SpeI ACTAGT 1 cut(s) 413
Sse9I AATT 9 cut(s) 289, 299, 343, 479, 569, 650, 691, 744, 813
SsiI CCGC 3 cut(s) 243, 246, 622
SspMI CTAG 3 cut(s) 414, 528, 647
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 831
TaaI ACNGT 1 cut(s) 504
TasI AATT 9 cut(s) 289, 299, 343, 479, 569, 650, 691, 744, 813
TauI GCSGC 2 cut(s) 246, 249
TfiI GAWTC 1 cut(s) 851
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 1 cut(s) 760
TseI GCWGC 1 cut(s) 97
TspDTI ATGAA 4 cut(s) 282, 473, 843, 887
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 1 cut(s) 760
Van91I CCANNNNNTGG 1 cut(s) 166
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 237, 350
XapI RAATTY 4 cut(s) 289, 343, 569, 813
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 781
XmiI GTMKAC 1 cut(s) 687
XspI CTAG 3 cut(s) 414, 528, 647
Zsp2I ATGCAT 2 cut(s) 472, 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.