Prupe.3G050100_v2.0.a1

3hydroxyisobutyrate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0016707
Physical Location & Seq
Reverse (-)
3534850 .. 3539054
4205 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G050100.1

Sequence Viewer

Length: 993 bp
ATGTTCTCTTTGTGCGCGTTCATCTTCTTCTTCACCTTCTCTCTCTTCATATTCTCCTTTTCTGTTTTCAAAATTCTGAGAAAAACAGAGCAAAAGCAACAGACCCAGAACGACCTTTTTGAGCTCAAGCGTTCATTGTCTGAAACTCTGGGCAAAACCCATCTAACCCATGAAAATGACCCGACCCATATCGCCAATCCTCCCCACAAGCTCCTCGTTGAGATCTTACCATCTGATTCTCCTAAATGGGCGAGCTTGTTTGTGAACAGAGAAGGTGGTGACCCGGATGACGCCGGGTCGGGTCTGGACGTGGAGAAACTCGGGTCGGGCGGGGATCAGAGAGTGAAGAAGAAGAAGAGGAGAGCCAAGAAGAAGAGTTCATCGCATTTGGAAGAAGAAGAAGAAGATAGTGGTTTTCATATGCGTGATAAGGAAAATTCGGGTCCGGGTTTGGGTTCTCGGGTCCATGAGGAATTGGTATGTTTTTACCCGTATACATCATCGAGTAGTGCTACGCAGAGGAAGATCAAGCAACAATACGATGAGCTTATGAAGTGCCATGAGTCCAGGAAATTGACACTTGCTCAGGTTGGACAATTTGCTAATTGCTTGGTGGAGGCTAGAAATGAGTTACAGCACAAGGCTGATGTCATTCAACGTAAATTCACAATAACAAAGGCTCTACTATTCAAGGCAGACAGATCTTCTTTTGACCGCCTTCGTCAACAGATATGCAAGCTAGAGTTAGAACAGAAGAGACTGGAAGAGGATGCGTCTGTCTACAATTGGCTTCAACAACAGCTTAAGCTTTCACCAGCATACAATAAGATGCTTGAAATTAGTGCCTGCATGGAGTTGAAGGCCAAATCCAGAGAACAAGTGGAGAGCACAGATGTGGAATATAGTGAAATATCATTTGAAGAGTTACTAGCACAAGAAAAGAAGGACTCATTCTGGCAGAAGAATAGGAAATCAAGATCACGCTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

38.18

Weight (kDa)

8.72

Isoelectric Point (pI)

61.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17665
fragaria_vesca FvH4_6g33270
malus_domestica MD17G1174400.v1.1
prunus_persica Prupe.3G050100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142971
rosa_laevigata RLG00000020005
rosa_multiflora Rmu_sc0002277.1_g000025
rosa_roxburghii Rroxscaffold_2G00102170
rosa_rugosa Rorug02G0377400
rosa_samantha Rh2AG430700 Rh2BG439600 Rh2CG416300 Rh2DG449600
rosa_wichuraiana Rw2G035170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 494, 780
AccII CGCG 1 cut(s) 17
AciI CCGC 2 cut(s) 330, 715
AclWI GGATC 1 cut(s) 342
AcsI RAATTY 3 cut(s) 72, 436, 662
AcyI GRCGYC 1 cut(s) 291
AfiI CCNNNNNNNGG 1 cut(s) 452
AflII CTTAAG 1 cut(s) 803
AgsI TTSAA 7 cut(s) 70, 656, 691, 794, 836, 859, 920
AhdI GACNNNNNGTC 1 cut(s) 295
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 1 cut(s) 566
AleI CACNNNNGTG 1 cut(s) 893
AluBI AGCT 8 cut(s) 124, 211, 255, 547, 739, 802, 808, 990
AluI AGCT 8 cut(s) 124, 211, 255, 547, 739, 802, 808, 990
Alw21I GWGCWC 2 cut(s) 126, 890
Alw26I GTCTC 1 cut(s) 751
AlwI GGATC 1 cut(s) 342
Ama87I CYCGRG 2 cut(s) 320, 459
AoxI GGCC 1 cut(s) 861
ApoI RAATTY 3 cut(s) 72, 436, 662
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 2 cut(s) 443, 463
AsuC2I CCSGG 3 cut(s) 284, 295, 447
AsuHPI GGTGA 3 cut(s) 25, 290, 804
AvaI CYCGRG 2 cut(s) 320, 459
AvaII GGWCC 2 cut(s) 443, 463
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 126, 890
BccI CCATC 2 cut(s) 168, 238
BciT130I CCWGG 1 cut(s) 568
BcnI CCSGG 3 cut(s) 284, 295, 447
BcoDI GTCTC 1 cut(s) 751
BfaI CTAG 3 cut(s) 621, 740, 929
BfrI CTTAAG 1 cut(s) 803
BglII AGATCT 2 cut(s) 222, 701
Bme1390I CCNGG 4 cut(s) 284, 295, 447, 568
Bme18I GGWCC 2 cut(s) 443, 463
BmeRI GACNNNNNGTC 1 cut(s) 295
BmeT110I CYCGRG 2 cut(s) 320, 459
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 443, 463
BmiI GGNNCC 2 cut(s) 444, 464
BmrFI CCNGG 4 cut(s) 284, 295, 447, 568
BmsI GCATC 2 cut(s) 760, 819
Bpu10I CCTNAGC 1 cut(s) 585
BpuEI CTTGAG 2 cut(s) 110, 970
BpuMI CCSGG 3 cut(s) 284, 295, 447
BsaHI GRCGYC 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse1I ACTGG 1 cut(s) 765
BseBI CCWGG 1 cut(s) 568
BseGI GGATG 2 cut(s) 292, 775
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMII CTCAG 2 cut(s) 68, 599
BseNI ACTGG 1 cut(s) 765
BseRI GAGGAG 2 cut(s) 203, 373
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 863
BsiHKAI GWGCWC 2 cut(s) 126, 890
BsiHKCI CYCGRG 2 cut(s) 320, 459
BsiSI CCGG 3 cut(s) 284, 294, 446
BslI CCNNNNNNNGG 1 cut(s) 452
BsmAI GTCTC 1 cut(s) 751
BsnI GGCC 1 cut(s) 863
BsoBI CYCGRG 2 cut(s) 320, 459
Bsp1286I GDGCHC 2 cut(s) 126, 890
Bsp143I GATC 5 cut(s) 222, 334, 525, 701, 977
BspACI CCGC 2 cut(s) 330, 715
BspANI GGCC 1 cut(s) 863
BspCNI CTCAG 2 cut(s) 69, 598
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 444, 464
BspPI GGATC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 803
BsrI ACTGG 1 cut(s) 765
BssMI GATC 5 cut(s) 222, 334, 525, 701, 977
BssNAI GTATAC 1 cut(s) 495
BssNI GRCGYC 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 568
Bst6I CTCTTC 6 cut(s) 50, 350, 368, 749, 759, 915
BstACI GRCGYC 1 cut(s) 291
BstAFI CTTAAG 1 cut(s) 803
BstC8I GCNNGC 3 cut(s) 253, 737, 847
BstDEI CTNAG 2 cut(s) 77, 585
BstEII GGTNACC 1 cut(s) 278
BstF5I GGATG 2 cut(s) 292, 775
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 5 cut(s) 225, 337, 528, 704, 980
BstMAI GTCTC 1 cut(s) 751
BstMBI GATC 5 cut(s) 222, 334, 525, 701, 977
BstNI CCWGG 1 cut(s) 568
BstPI GGTNACC 1 cut(s) 278
BstSCI CCNGG 4 cut(s) 282, 293, 445, 566
BstUI CGCG 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 222, 701
BstYI RGATCY 2 cut(s) 222, 701
BstZ17I GTATAC 1 cut(s) 495
BsuRI GGCC 1 cut(s) 863
BtgZI GCGATG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 2 cut(s) 292, 775
Cac8I GCNNGC 3 cut(s) 253, 737, 847
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 443, 463
CseI GACGC 2 cut(s) 299, 762
CviAII CATG 4 cut(s) 170, 467, 560, 850
DdeI CTNAG 2 cut(s) 77, 585
DpnI GATC 5 cut(s) 224, 336, 527, 703, 979
DpnII GATC 5 cut(s) 222, 334, 525, 701, 977
DriI GACNNNNNGTC 1 cut(s) 295
Eam1104I CTCTTC 6 cut(s) 50, 350, 368, 749, 759, 915
Eam1105I GACNNNNNGTC 1 cut(s) 295
EarI CTCTTC 6 cut(s) 50, 350, 368, 749, 759, 915
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 2 cut(s) 443, 463
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 2 cut(s) 320, 459
Eco91I GGTNACC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 124
EcoO65I GGTNACC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 566
EcoT38I GRGCYC 1 cut(s) 126
FaeI CATG 4 cut(s) 173, 470, 563, 853
FatI CATG 4 cut(s) 169, 466, 559, 849
FauI CCCGC 1 cut(s) 323
FauNDI CATATG 1 cut(s) 420
FblI GTMKAC 2 cut(s) 494, 780
FokI GGATG 2 cut(s) 299, 782
FriOI GRGCYC 1 cut(s) 126
FspBI CTAG 3 cut(s) 621, 740, 929
GlaI GCGC 1 cut(s) 16
HaeIII GGCC 1 cut(s) 863
HapII CCGG 3 cut(s) 284, 294, 446
HgaI GACGC 2 cut(s) 299, 762
HhaI GCGC 1 cut(s) 17
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 4 cut(s) 173, 470, 563, 853
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HincII GTYRAC 1 cut(s) 725
HindII GTYRAC 1 cut(s) 725
HindIII AAGCTT 1 cut(s) 806
HinfI GANTC 3 cut(s) 236, 563, 947
HpaII CCGG 3 cut(s) 284, 294, 446
HphI GGTGA 3 cut(s) 25, 290, 804
Hpy166II GTNNAC 4 cut(s) 265, 495, 725, 781
Hpy188I TCNGA 4 cut(s) 78, 142, 235, 339
Hpy188III TCNNGA 3 cut(s) 305, 870, 975
Hpy8I GTNNAC 4 cut(s) 265, 495, 725, 781
HpyAV CCTTC 5 cut(s) 46, 266, 728, 853, 937
HpyCH4IV ACGT 2 cut(s) 309, 658
HpyCH4V TGCA 2 cut(s) 735, 849
HpyF3I CTNAG 2 cut(s) 77, 585
HpySE526I ACGT 2 cut(s) 309, 658
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 4 cut(s) 173, 470, 563, 853
HspAI GCGC 1 cut(s) 15
Kzo9I GATC 5 cut(s) 222, 334, 525, 701, 977
LmnI GCTCC 1 cut(s) 216
LweI GCATC 2 cut(s) 760, 819
MaeI CTAG 3 cut(s) 621, 740, 929
MaeII ACGT 2 cut(s) 309, 658
MaeIII GTNAC 3 cut(s) 278, 630, 924
MalI GATC 5 cut(s) 224, 336, 527, 703, 979
MboI GATC 5 cut(s) 222, 334, 525, 701, 977
MfeI CAATTG 1 cut(s) 784
MflI RGATCY 2 cut(s) 222, 701
MhlI GDGCHC 2 cut(s) 126, 890
MluCI AATT 9 cut(s) 72, 436, 473, 572, 596, 604, 662, 784, 837
MlyI GAGTC 2 cut(s) 572, 941
MmeI TCCRAC 1 cut(s) 571
MnlI CCTC 7 cut(s) 210, 224, 351, 463, 513, 610, 760
MseI TTAA 1 cut(s) 804
MslI CAYNNNNRTG 3 cut(s) 174, 423, 893
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 3 cut(s) 284, 294, 446
MspR9I CCNGG 4 cut(s) 284, 295, 447, 568
MunI CAATTG 1 cut(s) 784
MvaI CCWGG 1 cut(s) 568
MvnI CGCG 1 cut(s) 17
NciI CCSGG 3 cut(s) 284, 295, 447
NdeI CATATG 1 cut(s) 420
NdeII GATC 5 cut(s) 222, 334, 525, 701, 977
NlaIII CATG 4 cut(s) 173, 470, 563, 853
NlaIV GGNNCC 2 cut(s) 444, 464
NmuCI GTSAC 1 cut(s) 278
OliI CACNNNNGTG 1 cut(s) 893
PfeI GAWTC 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 566
PleI GAGTC 2 cut(s) 571, 941
PpsI GAGTC 2 cut(s) 571, 941
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 566
PspEI GGTNACC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 566
PspN4I GGNNCC 2 cut(s) 444, 464
PspPI GGNCC 2 cut(s) 443, 463
PsuI RGATCY 2 cut(s) 222, 701
RseI CAYNNNNRTG 3 cut(s) 174, 423, 893
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 804
Sau3AI GATC 5 cut(s) 222, 334, 525, 701, 977
Sau96I GGNCC 2 cut(s) 443, 463
SchI GAGTC 2 cut(s) 572, 941
ScrFI CCNGG 4 cut(s) 284, 295, 447, 568
SduI GDGCHC 2 cut(s) 126, 890
SfaNI GCATC 2 cut(s) 760, 819
SinI GGWCC 2 cut(s) 443, 463
SmiMI CAYNNNNRTG 3 cut(s) 174, 423, 893
SmlI CTYRAG 3 cut(s) 125, 803, 985
SmoI CTYRAG 3 cut(s) 125, 803, 985
Sse9I AATT 9 cut(s) 72, 436, 473, 572, 596, 604, 662, 784, 837
SsiI CCGC 2 cut(s) 330, 715
SspMI CTAG 3 cut(s) 621, 740, 929
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 4 cut(s) 282, 293, 445, 566
TaiI ACGT 2 cut(s) 312, 661
TaqI TCGA 1 cut(s) 503
TasI AATT 9 cut(s) 72, 436, 473, 572, 596, 604, 662, 784, 837
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 804
Tru9I TTAA 1 cut(s) 804
TseFI GTSAC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 7 cut(s) 10, 37, 123, 186, 369, 407, 566
Vha464I CTTAAG 1 cut(s) 803
VpaK11BI GGWCC 2 cut(s) 443, 463
XapI RAATTY 3 cut(s) 72, 436, 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XmiI GTMKAC 2 cut(s) 494, 780
XspI CTAG 3 cut(s) 621, 740, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.