Rroxscaffold_2G00102170

3hydroxyisobutyrate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016707
Physical Location & Seq
Reverse (-)
24200519 .. 24204132
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00102170.1

Sequence Viewer

Length: 885 bp
ATGTCCAATCTGGGCCTCTTCGTCTTCCTCTTCACTTTCTTCATCGCCATCCCTTTCTTTGTTTTCGTAAAGCTGAGGAAAACAGAGCACAAGCAACAGACCCAGAATGACCTGTTTGAACTAAAGCGGTTTCTGTCCGAGACATTGGCCGAGACCAGTCAGGTAGGCAATGAAAATGACCCGACCCATTTGGCCCAAAAAGGGTCGGGCTCGGAGGACGAGGAGAGGGAAGGGTCCGGCGGCAATGAGAAGGTGAAAAAGAAGAGGAGGGCCAAGAAGAAGAATACATCACATTTGGAACAAGATGGTGGTGGTGGGGAGGATATGAGTGAGAAAGGGAATTCTGGTCCTCAGATCAAGGCTGATGTGGTGTGTTTCTACCCGTTTACGTCATCCACTAGTGCTACACAAAGAAAGATCAAGAAACAATATGATGAGCTTATGAAGTGTAATGCATCCAAGAAATTGACTCTCTCTCAGGTTGGACAGTTTGCTACTTGCTTAGTTGAGGCTAGAAATGAGCTACAGCACAAGGCTGATGCCATTCAGCGTAAATTCACAATAAGAAAGGCTTTACTATACAAGGCAGATAGATCTTCTTTTGACCGCCTTCGTAAACAGATATGCAAGCTAGAATTAGAACAGAAGAGACTGGAAGAGGATGCATCTGTCTACAATTGGCTCCAACAACAGCTTAAGCTTTCACCAGCATACAAGAAGATGCTTGAAATTAGTGCCTGCACGGAGTTGAAGACCAAATCCAGAGAGCAAATGGAAAGTAAAGATGTAGATTTTACTGGAATTTCATTTGAAGATTTCCTGGCACAAGAAAAGAAGGATTCATTCTGGCAGAAAAACATGAAATCAAGATCATGCTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

33.75

Weight (kDa)

9.24

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17665
fragaria_vesca FvH4_6g33270
malus_domestica MD17G1174400.v1.1
prunus_persica Prupe.3G050100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142971
rosa_laevigata RLG00000020005
rosa_multiflora Rmu_sc0002277.1_g000025
rosa_roxburghii Rroxscaffold_2G00102170
rosa_rugosa Rorug02G0377400
rosa_samantha Rh2AG430700 Rh2BG439600 Rh2CG416300 Rh2DG449600
rosa_wichuraiana Rw2G035170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 672
AciI CCGC 3 cut(s) 127, 240, 607
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 3 cut(s) 340, 554, 801
AfiI CCNNNNNNNGG 1 cut(s) 201
AflII CTTAAG 1 cut(s) 695
AgsI TTSAA 4 cut(s) 119, 728, 751, 812
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 1 cut(s) 819
AluBI AGCT 7 cut(s) 73, 439, 523, 631, 694, 700, 882
AluI AGCT 7 cut(s) 73, 439, 523, 631, 694, 700, 882
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 3 cut(s) 134, 146, 643
AoxI GGCC 4 cut(s) 13, 147, 192, 270
ApoI RAATTY 3 cut(s) 340, 554, 801
AspS9I GGNCC 5 cut(s) 13, 193, 234, 270, 347
AsuHPI GGTGA 2 cut(s) 265, 696
AvaII GGWCC 2 cut(s) 234, 347
BanII GRGCYC 1 cut(s) 212
BbsI GAAGAC 2 cut(s) 16, 758
Bbv12I GWGCWC 1 cut(s) 90
BbvCI CCTCAGC 1 cut(s) 74
BccI CCATC 2 cut(s) 56, 299
BciT130I CCWGG 1 cut(s) 821
BcoDI GTCTC 3 cut(s) 134, 146, 643
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 3 cut(s) 399, 513, 632
BfmI CTRYAG 1 cut(s) 524
BfrI CTTAAG 1 cut(s) 695
BglII AGATCT 1 cut(s) 593
BisI GCNGC 1 cut(s) 241
BlsI GCNGC 1 cut(s) 242
Bme1390I CCNGG 1 cut(s) 821
Bme18I GGWCC 2 cut(s) 234, 347
BmgT120I GGNCC 5 cut(s) 13, 193, 234, 270, 347
BmiI GGNNCC 2 cut(s) 235, 683
BmrFI CCNGG 1 cut(s) 821
BmsI GCATC 5 cut(s) 464, 529, 652, 674, 711
BpiI GAAGAC 2 cut(s) 16, 758
Bpu10I CCTNAGC 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 862
BsaI GGTCTC 1 cut(s) 146
Bsc4I CCNNNNNNNGG 1 cut(s) 201
Bse1I ACTGG 3 cut(s) 156, 657, 802
Bse3DI GCAATG 2 cut(s) 175, 250
BseBI CCWGG 1 cut(s) 821
BseGI GGATG 4 cut(s) 48, 392, 455, 667
BseLI CCNNNNNNNGG 1 cut(s) 201
BseMI GCAATG 2 cut(s) 175, 250
BseMII CTCAG 3 cut(s) 65, 365, 491
BseNI ACTGG 3 cut(s) 156, 657, 802
BseRI GAGGAG 2 cut(s) 236, 280
BsgI GTGCAG 1 cut(s) 724
BshFI GGCC 4 cut(s) 15, 149, 194, 272
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 201
BsmAI GTCTC 3 cut(s) 134, 146, 643
BsnI GGCC 4 cut(s) 15, 149, 194, 272
Bso31I GGTCTC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 90, 212
Bsp143I GATC 4 cut(s) 354, 417, 593, 869
BspACI CCGC 3 cut(s) 127, 240, 607
BspANI GGCC 4 cut(s) 15, 149, 194, 272
BspCNI CTCAG 3 cut(s) 66, 364, 490
BspLI GGNNCC 2 cut(s) 235, 683
BspTI CTTAAG 1 cut(s) 695
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 2 cut(s) 175, 250
BsrI ACTGG 3 cut(s) 156, 657, 802
BssMI GATC 4 cut(s) 354, 417, 593, 869
Bst2UI CCWGG 1 cut(s) 821
Bst4CI ACNGT 1 cut(s) 489
Bst6I CTCTTC 5 cut(s) 23, 35, 257, 641, 651
BstAFI CTTAAG 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 629, 739
BstDEI CTNAG 4 cut(s) 74, 351, 477, 502
BstF5I GGATG 4 cut(s) 48, 392, 455, 667
BstKTI GATC 4 cut(s) 357, 420, 596, 872
BstMAI GTCTC 3 cut(s) 134, 146, 643
BstMBI GATC 4 cut(s) 354, 417, 593, 869
BstNI CCWGG 1 cut(s) 821
BstSCI CCNGG 1 cut(s) 819
BstSFI CTRYAG 1 cut(s) 524
BstV2I GAAGAC 2 cut(s) 16, 758
BstX2I RGATCY 1 cut(s) 593
BstYI RGATCY 1 cut(s) 593
BsuRI GGCC 4 cut(s) 15, 149, 194, 272
BtgZI GCGATG 1 cut(s) 28
BtsCI GGATG 4 cut(s) 48, 392, 455, 667
Cac8I GCNNGC 2 cut(s) 629, 739
Cfr13I GGNCC 5 cut(s) 13, 193, 234, 270, 347
CviAII CATG 2 cut(s) 859, 873
DdeI CTNAG 4 cut(s) 74, 351, 477, 502
DpnI GATC 4 cut(s) 356, 419, 595, 871
DpnII GATC 4 cut(s) 354, 417, 593, 869
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 5 cut(s) 23, 35, 257, 641, 651
EarI CTCTTC 5 cut(s) 23, 35, 257, 641, 651
Eco24I GRGCYC 1 cut(s) 212
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 2 cut(s) 234, 347
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 819
EcoT22I ATGCAT 2 cut(s) 457, 667
EcoT38I GRGCYC 1 cut(s) 212
FaeI CATG 2 cut(s) 862, 876
FaiI YATR 8 cut(s) 326, 432, 443, 580, 625, 712, 860, 874
FalI AAGNNNNNCTT 2 cut(s) 556, 588
FatI CATG 2 cut(s) 858, 872
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 1 cut(s) 241
FokI GGATG 4 cut(s) 35, 379, 442, 674
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 1 cut(s) 241
FspBI CTAG 3 cut(s) 399, 513, 632
GluI GCNGC 1 cut(s) 241
HaeIII GGCC 4 cut(s) 15, 149, 194, 272
HapII CCGG 1 cut(s) 237
Hin1II CATG 2 cut(s) 862, 876
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 2 cut(s) 469, 839
HpaII CCGG 1 cut(s) 237
HphI GGTGA 2 cut(s) 265, 696
Hpy166II GTNNAC 3 cut(s) 387, 617, 673
Hpy188I TCNGA 3 cut(s) 139, 214, 354
Hpy188III TCNNGA 3 cut(s) 421, 762, 867
Hpy8I GTNNAC 3 cut(s) 387, 617, 673
HpyAV CCTTC 4 cut(s) 224, 244, 620, 829
HpyCH4III ACNGT 1 cut(s) 489
HpyCH4IV ACGT 1 cut(s) 389
HpyCH4V TGCA 4 cut(s) 455, 627, 665, 741
HpyF3I CTNAG 4 cut(s) 74, 351, 477, 502
HpySE526I ACGT 1 cut(s) 389
Hsp92II CATG 2 cut(s) 862, 876
Kzo9I GATC 4 cut(s) 354, 417, 593, 869
LmnI GCTCC 1 cut(s) 687
LweI GCATC 5 cut(s) 464, 529, 652, 674, 711
MaeI CTAG 3 cut(s) 399, 513, 632
MaeII ACGT 1 cut(s) 389
MalI GATC 4 cut(s) 356, 419, 595, 871
MboI GATC 4 cut(s) 354, 417, 593, 869
MfeI CAATTG 1 cut(s) 676
MflI RGATCY 1 cut(s) 593
MhlI GDGCHC 2 cut(s) 90, 212
MluCI AATT 7 cut(s) 340, 464, 554, 635, 676, 729, 801
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 2 cut(s) 463, 709
Mph1103I ATGCAT 2 cut(s) 457, 667
MseI TTAA 1 cut(s) 696
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 1 cut(s) 821
MunI CAATTG 1 cut(s) 676
MvaI CCWGG 1 cut(s) 821
NdeII GATC 4 cut(s) 354, 417, 593, 869
NlaIII CATG 2 cut(s) 862, 876
NlaIV GGNNCC 2 cut(s) 235, 683
NmeAIII GCCGAG 1 cut(s) 175
NsiI ATGCAT 2 cut(s) 457, 667
PfeI GAWTC 1 cut(s) 839
PkrI GCNGC 1 cut(s) 242
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 819
PspGI CCWGG 1 cut(s) 819
PspN4I GGNNCC 2 cut(s) 235, 683
PspPI GGNCC 5 cut(s) 13, 193, 234, 270, 347
PsuI RGATCY 1 cut(s) 593
SaqAI TTAA 1 cut(s) 696
SatI GCNGC 1 cut(s) 241
Sau3AI GATC 4 cut(s) 354, 417, 593, 869
Sau96I GGNCC 5 cut(s) 13, 193, 234, 270, 347
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 1 cut(s) 821
SduI GDGCHC 2 cut(s) 90, 212
SfaNI GCATC 5 cut(s) 464, 529, 652, 674, 711
SfcI CTRYAG 1 cut(s) 524
SinI GGWCC 2 cut(s) 234, 347
SmlI CTYRAG 2 cut(s) 695, 877
SmoI CTYRAG 2 cut(s) 695, 877
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 7 cut(s) 340, 464, 554, 635, 676, 729, 801
SsiI CCGC 3 cut(s) 127, 240, 607
SspMI CTAG 3 cut(s) 399, 513, 632
StyD4I CCNGG 1 cut(s) 819
TaaI ACNGT 1 cut(s) 489
TaiI ACGT 1 cut(s) 392
TasI AATT 7 cut(s) 340, 464, 554, 635, 676, 729, 801
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 1 cut(s) 839
Tru1I TTAA 1 cut(s) 696
Tru9I TTAA 1 cut(s) 696
TspDTI ATGAA 6 cut(s) 31, 186, 458, 795, 831, 875
TspGWI ACGGA 1 cut(s) 758
Vha464I CTTAAG 1 cut(s) 695
VpaK11BI GGWCC 2 cut(s) 234, 347
XapI RAATTY 3 cut(s) 340, 554, 801
XcmI CCANNNNNNNNNTGG 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 672
XspI CTAG 3 cut(s) 399, 513, 632
Zsp2I ATGCAT 2 cut(s) 457, 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.