MD17G1174400.v1.1

3hydroxyisobutyrate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0016707
Physical Location & Seq
Reverse (-)
19405027 .. 19408571
3545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1174400.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGCGGCTTCACATTCGTATCTATCCAATCTGGCTTCCAAATTTCCAATCCCAATACAGTGTCAGTCCCAGTTCCATACGCAAACACCCATGTTATCTCTGTGCACCTTCGTGTTCCTCGTCACCTTCTCTCTCTTCCTCTTCTCCTTCTCTGTTTTCAAAAAGTTGCAGAAAACAGAGCACAAGCAGCAGACCTACGACGACCTTTTCGAGCTCAAACGTTCATTGTCCCAAACTCTGGGCAAGGCCCATCCGGCCCGTGAAAATAACCCGACCCATTTCGACAATCCGCCCCACAAAATCCTCGTGGAGATCCTGCCCTCTGATTCTTCCAAATGGGCGAGCTTGTTCGTGAACAATGAGGGTGGCGGCCCGGATAACGCCGGGTTGGGTTTGGACGGGGATAAAGTCGGGTCGGAGGGGCATAAGGGAGCTAAGAAGAAGAAGAGGAGGGCCAAGAAGAAGAAGTTCTCGAATTTGGAAGAGGAAGAAGAAGATGGGAGTGAAGATTTGTGCGGTCAGGGGAATTCGAGTCCGGGTTTGGGCTCTCGGGTGAAGGAGGAATTGGTATGTTTTTACCCGTTTACATCATCGAGTAGTGCTACTCAGAGGAAGATCAAGCAGCAATACGATGAGCTCATGAAGTGCCATGAGTCCGAGAAATTGACACTCGCTCAGGTTGGACAGTTTGCTAATTGCTTGGTGGACGCTAGAAATGAGCTACAGCACAAAGCTGATGTCATTCAACGTAAATTCACAATAACAAAAGCTCTCTTATTCAAGGCAGACAGATCTTCTTTTGACCGCCTTCATCAACAGATGTGCAAGCTAGAGTTAGAACAGAAGAGACTGGAAGAGGATGCATCTGTCTACAATTGGCTCCAACAACAGCTTAAGCTTTCACCAGCATACAAGAAGATGTTTGAAATTAGTACCCACATCGACTTGAAAGCCAAATCCAGAGAACAAATGGAGAGCACAGATGTGGAATTTACTGAAATATCATTTGAAGAGTTACTAGCACAAGAAAAGAAGGATTCATTCTGGCAGAAAAATAGGAAATCAAGATCATGCTCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

361

Amino Acids

41.11

Weight (kDa)

8.61

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17665
fragaria_vesca FvH4_6g33270
malus_domestica MD17G1174400.v1.1
prunus_persica Prupe.3G050100_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0142971
rosa_laevigata RLG00000020005
rosa_multiflora Rmu_sc0002277.1_g000025
rosa_roxburghii Rroxscaffold_2G00102170
rosa_rugosa Rorug02G0377400
rosa_samantha Rh2AG430700 Rh2BG439600 Rh2CG416300 Rh2DG449600
rosa_wichuraiana Rw2G035170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 238
AccI GTMKAC 1 cut(s) 870
AciI CCGC 5 cut(s) 5, 290, 369, 516, 805
AclI AACGTT 1 cut(s) 220
AclWI GGATC 1 cut(s) 307
AcsI RAATTY 5 cut(s) 41, 475, 526, 752, 989
AfaI GTAC 1 cut(s) 934
AfiI CCNNNNNNNGG 2 cut(s) 238, 542
AflII CTTAAG 1 cut(s) 893
AgsI TTSAA 6 cut(s) 160, 746, 781, 926, 949, 1010
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
AleI CACNNNNGTG 2 cut(s) 110, 983
Alw21I GWGCWC 5 cut(s) 107, 183, 216, 639, 980
Alw26I GTCTC 1 cut(s) 841
Alw44I GTGCAC 1 cut(s) 103
AlwI GGATC 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 549
AoxI GGCC 4 cut(s) 246, 255, 370, 453
ApaLI GTGCAC 1 cut(s) 103
ApeKI GCWGC 2 cut(s) 187, 622
ApoI RAATTY 5 cut(s) 41, 475, 526, 752, 989
ArsI GACNNNNNNTTYG 4 cut(s) 191, 223, 723, 755
Asp700I GAANNNNTTC 1 cut(s) 467
AspS9I GGNCC 4 cut(s) 247, 256, 371, 453
AsuC2I CCSGG 3 cut(s) 374, 385, 537
AsuHPI GGTGA 3 cut(s) 115, 565, 894
AvaI CYCGRG 1 cut(s) 549
BaeGI GKGCMC 1 cut(s) 107
BaeI ACNNNNGTAYC 1 cut(s) 35
BanII GRGCYC 3 cut(s) 216, 548, 639
BauI CACGAG 1 cut(s) 305
Bbv12I GWGCWC 5 cut(s) 107, 183, 216, 639, 980
BbvI GCAGC 2 cut(s) 199, 634
BccI CCATC 2 cut(s) 258, 491
BcnI CCSGG 3 cut(s) 374, 385, 537
BcoDI GTCTC 1 cut(s) 841
BfaI CTAG 4 cut(s) 711, 830, 1019, 1077
BfmI CTRYAG 1 cut(s) 722
BfrI CTTAAG 1 cut(s) 893
BglI GCCNNNNNGGC 1 cut(s) 254
BglII AGATCT 1 cut(s) 791
BisI GCNGC 4 cut(s) 6, 188, 370, 623
BlsI GCNGC 4 cut(s) 7, 189, 371, 624
Bme1390I CCNGG 3 cut(s) 374, 385, 537
BmeT110I CYCGRG 1 cut(s) 549
BmgT120I GGNCC 4 cut(s) 247, 256, 371, 453
BmiI GGNNCC 1 cut(s) 881
BmrFI CCNGG 3 cut(s) 374, 385, 537
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 850, 872
BmuI ACTGGG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 675
BpuMI CCSGG 3 cut(s) 374, 385, 537
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 542
Bse1I ACTGG 2 cut(s) 70, 855
BseGI GGATG 2 cut(s) 250, 865
BseLI CCNNNNNNNGG 2 cut(s) 238, 542
BseMII CTCAG 2 cut(s) 620, 689
BseNI ACTGG 2 cut(s) 70, 855
BseRI GAGGAG 1 cut(s) 463
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 2 cut(s) 199, 634
BshFI GGCC 4 cut(s) 248, 257, 372, 455
BsiHKAI GWGCWC 5 cut(s) 107, 183, 216, 639, 980
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 4 cut(s) 254, 374, 384, 536
BslFI GGGAC 2 cut(s) 52, 214
BslI CCNNNNNNNGG 2 cut(s) 238, 542
BsmAI GTCTC 1 cut(s) 841
BsmFI GGGAC 2 cut(s) 52, 214
BsnI GGCC 4 cut(s) 248, 257, 372, 455
BsoBI CYCGRG 1 cut(s) 549
Bsp1286I GDGCHC 6 cut(s) 107, 183, 216, 548, 639, 980
Bsp143I GATC 4 cut(s) 312, 615, 791, 1067
BspACI CCGC 5 cut(s) 5, 290, 369, 516, 805
BspANI GGCC 4 cut(s) 248, 257, 372, 455
BspCNI CTCAG 2 cut(s) 619, 688
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 1 cut(s) 881
BspPI GGATC 1 cut(s) 307
BspTI CTTAAG 1 cut(s) 893
BsrI ACTGG 2 cut(s) 70, 855
BssMI GATC 4 cut(s) 312, 615, 791, 1067
BssSI CACGAG 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 60, 687
Bst6I CTCTTC 7 cut(s) 140, 146, 440, 477, 839, 849, 1005
BstAFI CTTAAG 1 cut(s) 893
BstC8I GCNNGC 2 cut(s) 343, 827
BstDEI CTNAG 3 cut(s) 435, 606, 675
BstF5I GGATG 2 cut(s) 250, 865
BstKTI GATC 4 cut(s) 315, 618, 794, 1070
BstMAI GTCTC 1 cut(s) 841
BstMBI GATC 4 cut(s) 312, 615, 791, 1067
BstMWI GCNNNNNNNGC 2 cut(s) 187, 254
BstSCI CCNGG 3 cut(s) 372, 383, 535
BstSFI CTRYAG 1 cut(s) 722
BstSLI GKGCMC 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 199, 634
BstX2I RGATCY 2 cut(s) 312, 791
BstYI RGATCY 2 cut(s) 312, 791
BsuRI GGCC 4 cut(s) 248, 257, 372, 455
BtsCI GGATG 2 cut(s) 250, 865
BtsIMutI CAGTG 1 cut(s) 65
Cac8I GCNNGC 2 cut(s) 343, 827
CciI TCATGA 1 cut(s) 639
Cfr13I GGNCC 4 cut(s) 247, 256, 371, 453
CseI GACGC 1 cut(s) 716
Csp6I GTAC 1 cut(s) 933
CspCI CAANNNNNGTGG 2 cut(s) 346, 381
CviAII CATG 4 cut(s) 91, 640, 650, 1071
CviQI GTAC 1 cut(s) 933
DdeI CTNAG 3 cut(s) 435, 606, 675
DpnI GATC 4 cut(s) 314, 617, 793, 1069
DpnII GATC 4 cut(s) 312, 615, 791, 1067
Eam1104I CTCTTC 7 cut(s) 140, 146, 440, 477, 839, 849, 1005
EarI CTCTTC 7 cut(s) 140, 146, 440, 477, 839, 849, 1005
EciI GGCGGA 1 cut(s) 279
Ecl136II GAGCTC 2 cut(s) 214, 637
Eco24I GRGCYC 3 cut(s) 216, 548, 639
Eco53kI GAGCTC 2 cut(s) 214, 637
Eco88I CYCGRG 1 cut(s) 549
EcoICRI GAGCTC 2 cut(s) 214, 637
EcoRI GAATTC 1 cut(s) 526
EcoT22I ATGCAT 1 cut(s) 865
EcoT38I GRGCYC 3 cut(s) 216, 548, 639
FaeI CATG 4 cut(s) 94, 643, 653, 1074
FaiI YATR 8 cut(s) 78, 92, 426, 571, 641, 651, 910, 1072
FaqI GGGAC 2 cut(s) 52, 214
FatI CATG 4 cut(s) 90, 639, 649, 1070
FblI GTMKAC 1 cut(s) 870
Fnu4HI GCNGC 4 cut(s) 6, 188, 370, 623
FokI GGATG 2 cut(s) 237, 872
FriOI GRGCYC 3 cut(s) 216, 548, 639
Fsp4HI GCNGC 4 cut(s) 6, 188, 370, 623
FspBI CTAG 4 cut(s) 711, 830, 1019, 1077
GluI GCNGC 4 cut(s) 6, 188, 370, 623
HaeIII GGCC 4 cut(s) 248, 257, 372, 455
HapII CCGG 4 cut(s) 254, 374, 384, 536
HgaI GACGC 1 cut(s) 716
Hin1II CATG 4 cut(s) 94, 643, 653, 1074
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 4 cut(s) 326, 532, 653, 1037
HpaII CCGG 4 cut(s) 254, 374, 384, 536
HphI GGTGA 3 cut(s) 115, 565, 894
Hpy166II GTNNAC 5 cut(s) 105, 355, 585, 706, 871
Hpy188I TCNGA 4 cut(s) 325, 418, 609, 658
Hpy188III TCNNGA 5 cut(s) 352, 472, 640, 960, 1065
Hpy8I GTNNAC 5 cut(s) 105, 355, 585, 706, 871
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 6 cut(s) 118, 136, 157, 550, 818, 1027
HpyCH4III ACNGT 2 cut(s) 60, 687
HpyCH4IV ACGT 2 cut(s) 220, 748
HpyCH4V TGCA 4 cut(s) 105, 169, 825, 863
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 254
HpyF3I CTNAG 3 cut(s) 435, 606, 675
HpySE526I ACGT 2 cut(s) 220, 748
Hsp92II CATG 4 cut(s) 94, 643, 653, 1074
Kzo9I GATC 4 cut(s) 312, 615, 791, 1067
LmnI GCTCC 2 cut(s) 431, 885
Lsp1109I GCAGC 2 cut(s) 199, 634
LweI GCATC 2 cut(s) 850, 872
MaeI CTAG 4 cut(s) 711, 830, 1019, 1077
MaeII ACGT 2 cut(s) 220, 748
MaeIII GTNAC 2 cut(s) 121, 1014
MalI GATC 4 cut(s) 314, 617, 793, 1069
MboI GATC 4 cut(s) 312, 615, 791, 1067
MfeI CAATTG 1 cut(s) 874
MflI RGATCY 2 cut(s) 312, 791
MhlI GDGCHC 6 cut(s) 107, 183, 216, 548, 639, 980
MlyI GAGTC 2 cut(s) 541, 662
MmeI TCCRAC 3 cut(s) 396, 661, 907
Mph1103I ATGCAT 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 1 cut(s) 894
MslI CAYNNNNRTG 2 cut(s) 110, 983
MspCI CTTAAG 1 cut(s) 893
MspI CCGG 4 cut(s) 254, 374, 384, 536
MspR9I CCNGG 3 cut(s) 374, 385, 537
MunI CAATTG 1 cut(s) 874
MwoI GCNNNNNNNGC 2 cut(s) 187, 254
NciI CCSGG 3 cut(s) 374, 385, 537
NdeII GATC 4 cut(s) 312, 615, 791, 1067
NlaIII CATG 4 cut(s) 94, 643, 653, 1074
NlaIV GGNNCC 1 cut(s) 881
NmuCI GTSAC 1 cut(s) 121
NsiI ATGCAT 1 cut(s) 865
OliI CACNNNNGTG 2 cut(s) 110, 983
PagI TCATGA 1 cut(s) 639
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 207
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 326, 1037
PflMI CCANNNNNTGG 1 cut(s) 238
PkrI GCNGC 4 cut(s) 7, 189, 371, 624
PleI GAGTC 2 cut(s) 540, 661
PpsI GAGTC 2 cut(s) 540, 661
Psp124BI GAGCTC 2 cut(s) 216, 639
Psp1406I AACGTT 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 881
PspPI GGNCC 4 cut(s) 247, 256, 371, 453
PsuI RGATCY 2 cut(s) 312, 791
RsaI GTAC 1 cut(s) 934
RsaNI GTAC 1 cut(s) 933
RseI CAYNNNNRTG 2 cut(s) 110, 983
SacI GAGCTC 2 cut(s) 216, 639
SaqAI TTAA 1 cut(s) 894
SatI GCNGC 4 cut(s) 6, 188, 370, 623
Sau3AI GATC 4 cut(s) 312, 615, 791, 1067
Sau96I GGNCC 4 cut(s) 247, 256, 371, 453
SchI GAGTC 2 cut(s) 541, 662
ScrFI CCNGG 3 cut(s) 374, 385, 537
SduI GDGCHC 6 cut(s) 107, 183, 216, 548, 639, 980
SfaNI GCATC 2 cut(s) 850, 872
SfcI CTRYAG 1 cut(s) 722
SfiI GGCCNNNNNGGCC 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 110, 983
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
SsiI CCGC 5 cut(s) 5, 290, 369, 516, 805
SspMI CTAG 4 cut(s) 711, 830, 1019, 1077
SstI GAGCTC 2 cut(s) 216, 639
StyD4I CCNGG 3 cut(s) 372, 383, 535
TaaI ACNGT 2 cut(s) 60, 687
TaiI ACGT 2 cut(s) 223, 751
TaqI TCGA 6 cut(s) 210, 282, 473, 530, 593, 942
TauI GCSGC 2 cut(s) 8, 372
TfiI GAWTC 2 cut(s) 326, 1037
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TscAI CASTG 1 cut(s) 65
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 2 cut(s) 187, 622
Tsp45I GTSAC 1 cut(s) 121
TspDTI ATGAA 4 cut(s) 213, 656, 800, 1029
TspRI CASTG 1 cut(s) 65
Van91I CCANNNNNTGG 1 cut(s) 238
Vha464I CTTAAG 1 cut(s) 893
VneI GTGCAC 1 cut(s) 103
XapI RAATTY 5 cut(s) 41, 475, 526, 752, 989
XcmI CCANNNNNNNNNTGG 1 cut(s) 967
XmiI GTMKAC 1 cut(s) 870
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 4 cut(s) 711, 830, 1019, 1077
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.