AT1G20490

ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004959
Physical Location & Seq
Reverse (-)
7097211 .. 7099892
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20490.2

Sequence Viewer

Length: 1344 bp
ATGGCGTATCCTGAGAGAGATCTCATTGTTGATCCTAGAAGCGGTTTCTGCAAATCAAACTCTACATTCTATAGCAAGCGAAACCCATTATGTCTCCCGCCAAATCCGTCGCTTGACGTCACAACTTTCATATCATCTCAACCGCAACGCGGCACCACCGCCTTCATCGACGCCTCCACGGGCCACCGCTTAACCTTCTCCGATCTCTGGAGAGTCGTTGATCGCGTCGCTGACTGTCTATATCATGAAGTCGGAATACGAAGAGGCGACGTCGTCCTCATCCTCTCTCCAAATTCAATCTACATTCCCGTCGTTTGCCTCTCCGTCATGTCTCTCGGCGCCGTCGTCACCACCGCCAACACTCTCAACACCTCCGGCGAAATCTCCAAACAGATCGCCCAAAGTAATCCAACTCTCGTCTTCACCACGAGCCAGCTGGCACCCAAACTCGCAGCCGCAATCTCCGTCGTCCTCACCGACGAAGAAGACGAGAAGCGAGTGGAGCTCACTAGTGGAGTCAGAGTCGTTGGGATTTTGAGCGAGATGATGAAGAAAGAAACGAGTGGTCAACGAGTCAGAGACCGAGTCAACCAGGACGACACGGCGATGATGCTTTACTCGTCGGGAACCACAGGGACGAGCAAAGGAGTGATCTCGTCTCACCGGAATTTAACAGCTTACGTTGCGAAATACATCGACGATAAATGGAAACGAGACGAGATATTCGTATGTACCGTGCCGATGTTCCACTCCTTCGGGTTATTGGCGTTCGCAATGGGCTCCGTAGCATCGGGTTCAACGGTGGTGATTCTCCGGAGATTCGGGCTCGACGATATGATGCAAGCGGTGGAGAAGTACAAAGCCACGATTCTGAGTTTGGCTCCACCGGTTTTGGTAGCTATGATTAACGGTGCAGATCAGCTCAAGGCCAAGTACGATTTGACCTCCCTGAGAAAGGTTAGGTGTGGTGGAGCACCGTTGAGCAAGGAGGTTATGGATAGCTTCCTAGAGAAATATCCAACGGTTAATATTTTTCAAGGATATGCGTTGACTGAGTCGCACGGTTCAGGAGCTTCGACGGAATCTGTGGAGGAGAGTCTGAAATACGGTGCGGTTGGGTTGTTGTCGTCCGGTATTGAAGCGAGGATTGTGGATCCGGATACGGGTCGGGTCATGGGTGTGAACCAACCCGGTGAGCTCTGGCTCAAGGGTCCTTCTATTTCTAAAGGTTATTTCGGAAATGAAGAAGCTACAAATGAAACTATTAATCTAGAAGGATGGCTAAAACTGGAGATCTCTGCTATATCGATGAAGATGGGTTTCTATTTGTTGTGGATCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01100 ko01110 map00592 map01100 map01110
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

447

Amino Acids

48.81

Weight (kDa)

6.06

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 49 - 412 2.1e-83 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 120, 273
AccB1I GGYRCC 3 cut(s) 152, 338, 439
AccII CGCG 2 cut(s) 150, 225
AccIII TCCGGA 2 cut(s) 813, 1156
AclWI GGATC 4 cut(s) 26, 1148, 1161, 1343
AcsI RAATTY 2 cut(s) 292, 667
AcyI GRCGYC 4 cut(s) 117, 171, 270, 339
AfaI GTAC 3 cut(s) 733, 857, 935
AfiI CCNNNNNNNGG 5 cut(s) 41, 149, 207, 955, 1163
AgeI ACCGGT 1 cut(s) 886
AgsI TTSAA 4 cut(s) 297, 798, 1037, 1139
AhlI ACTAGT 1 cut(s) 509
AjnI CCWGG 1 cut(s) 591
AluBI AGCT 9 cut(s) 436, 505, 677, 899, 922, 1002, 1073, 1198, 1250
AluI AGCT 9 cut(s) 436, 505, 677, 899, 922, 1002, 1073, 1198, 1250
Alw21I GWGCWC 3 cut(s) 507, 976, 1200
Alw26I GTCTC 5 cut(s) 98, 336, 573, 663, 708
AlwI GGATC 4 cut(s) 26, 1148, 1161, 1343
Aor13HI TCCGGA 2 cut(s) 813, 1156
AoxI GGCC 2 cut(s) 181, 927
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 2 cut(s) 292, 667
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AseI ATTAAT 1 cut(s) 1266
AsiGI ACCGGT 1 cut(s) 886
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 2 cut(s) 181, 1211
AsuC2I CCSGG 1 cut(s) 1191
AsuHPI GGTGA 6 cut(s) 340, 415, 466, 653, 817, 1205
AvaII GGWCC 1 cut(s) 1211
BamHI GGATCC 1 cut(s) 1153
BanI GGYRCC 3 cut(s) 152, 338, 439
BanII GRGCYC 4 cut(s) 507, 782, 828, 1200
BarI GAAGNNNNNNTAC 2 cut(s) 240, 272
BauI CACGAG 1 cut(s) 427
BbsI GAAGAC 2 cut(s) 412, 492
Bbv12I GWGCWC 3 cut(s) 507, 976, 1200
BbvI GCAGC 1 cut(s) 464
BccI CCATC 2 cut(s) 1272, 1309
BceAI ACGGC 2 cut(s) 326, 618
BciT130I CCWGG 1 cut(s) 593
BciVI GTATCC 2 cut(s) 18, 1153
BcnI CCSGG 1 cut(s) 1191
BcoDI GTCTC 5 cut(s) 98, 336, 573, 663, 708
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 4 cut(s) 36, 510, 1007, 1271
BfmI CTRYAG 1 cut(s) 70
BfoI RGCGCY 1 cut(s) 342
BfuI GTATCC 2 cut(s) 18, 1153
BglII AGATCT 2 cut(s) 19, 1293
BisI GCNGC 3 cut(s) 151, 453, 456
BlsI GCNGC 3 cut(s) 152, 454, 457
Bme1390I CCNGG 2 cut(s) 593, 1191
Bme18I GGWCC 1 cut(s) 1211
BmgT120I GGNCC 2 cut(s) 181, 1211
BmiI GGNNCC 8 cut(s) 154, 340, 441, 628, 781, 882, 1155, 1212
BmrFI CCNGG 2 cut(s) 593, 1191
BmsI GCATC 3 cut(s) 600, 797, 828
BpiI GAAGAC 2 cut(s) 412, 492
BplI GAGNNNNNCTC 4 cut(s) 489, 521, 865, 897
BpmI CTGGAG 2 cut(s) 229, 1310
BpuEI CTTGAG 2 cut(s) 908, 1190
BpuMI CCSGG 1 cut(s) 1191
Bsa29I ATCGAT 1 cut(s) 1307
BsaHI GRCGYC 4 cut(s) 117, 171, 270, 339
BsaI GGTCTC 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 177
BsaWI WCCGGW 5 cut(s) 663, 813, 886, 1130, 1156
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 149, 207, 955, 1163
Bse118I RCCGGY 1 cut(s) 886
Bse1I ACTGG 1 cut(s) 1293
Bse3DI GCAATG 1 cut(s) 780
BseAI TCCGGA 2 cut(s) 813, 1156
BseBI CCWGG 1 cut(s) 593
BseCI ATCGAT 1 cut(s) 1307
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 2 cut(s) 279, 1283
BseLI CCNNNNNNNGG 5 cut(s) 41, 149, 207, 955, 1163
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 3 cut(s) 863, 941, 1044
BseNI ACTGG 1 cut(s) 1293
BseRI GAGGAG 1 cut(s) 1106
BseXI GCAGC 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 2 cut(s) 150, 225
BshFI GGCC 2 cut(s) 183, 929
BshNI GGYRCC 3 cut(s) 152, 338, 439
BshTI ACCGGT 1 cut(s) 886
BshVI ATCGAT 1 cut(s) 1307
BsiHKAI GWGCWC 3 cut(s) 507, 976, 1200
BsiSI CCGG 7 cut(s) 375, 664, 814, 887, 1131, 1157, 1191
BslFI GGGAC 1 cut(s) 649
BslI CCNNNNNNNGG 5 cut(s) 41, 149, 207, 955, 1163
BsmAI GTCTC 5 cut(s) 98, 336, 573, 663, 708
BsmBI CGTCTC 2 cut(s) 663, 708
BsmFI GGGAC 1 cut(s) 649
BsnI GGCC 2 cut(s) 183, 929
Bso31I GGTCTC 1 cut(s) 573
Bsp1286I GDGCHC 5 cut(s) 507, 782, 828, 976, 1200
Bsp13I TCCGGA 2 cut(s) 813, 1156
BspANI GGCC 2 cut(s) 183, 929
BspCNI CTCAG 4 cut(s) 4, 864, 942, 1045
BspDI ATCGAT 1 cut(s) 1307
BspEI TCCGGA 2 cut(s) 813, 1156
BspFNI CGCG 2 cut(s) 150, 225
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 8 cut(s) 154, 340, 441, 628, 781, 882, 1155, 1212
BspPI GGATC 4 cut(s) 26, 1148, 1161, 1343
BspT107I GGYRCC 3 cut(s) 152, 338, 439
BspTNI GGTCTC 1 cut(s) 573
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 886
BsrI ACTGG 1 cut(s) 1293
BssAI RCCGGY 1 cut(s) 886
BssECI CCNNGG 1 cut(s) 177
BssNI GRCGYC 4 cut(s) 117, 171, 270, 339
BssSI CACGAG 1 cut(s) 427
Bst2BI CACGAG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 593
Bst4CI ACNGT 8 cut(s) 236, 736, 802, 911, 978, 1024, 1064, 1109
Bst6I CTCTTC 1 cut(s) 256
BstACI GRCGYC 4 cut(s) 117, 171, 270, 339
BstC8I GCNNGC 4 cut(s) 77, 434, 438, 843
BstDEI CTNAG 4 cut(s) 12, 872, 950, 1053
BstDSI CCRYGG 1 cut(s) 177
BstENI CCTNNNNNAGG 1 cut(s) 953
BstF5I GGATG 2 cut(s) 279, 1283
BstFNI CGCG 2 cut(s) 150, 225
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 1 cut(s) 341
BstMAI GTCTC 5 cut(s) 98, 336, 573, 663, 708
BstMWI GCNNNNNNNGC 3 cut(s) 48, 502, 683
BstNI CCWGG 1 cut(s) 593
BstSCI CCNGG 2 cut(s) 591, 1189
BstSFI CTRYAG 1 cut(s) 70
BstUI CGCG 2 cut(s) 150, 225
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 2 cut(s) 412, 492
BstX2I RGATCY 3 cut(s) 19, 1153, 1293
BstYI RGATCY 3 cut(s) 19, 1153, 1293
Bsu15I ATCGAT 1 cut(s) 1307
BsuI GTATCC 2 cut(s) 18, 1153
BsuRI GGCC 2 cut(s) 183, 929
BsuTUI ATCGAT 1 cut(s) 1307
BtgI CCRYGG 1 cut(s) 177
BtgZI GCGATG 1 cut(s) 620
BtsCI GGATG 2 cut(s) 279, 1283
Cac8I GCNNGC 4 cut(s) 77, 434, 438, 843
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 886
Cfr13I GGNCC 2 cut(s) 181, 1211
ClaI ATCGAT 1 cut(s) 1307
CseI GACGC 2 cut(s) 179, 214
Csp6I GTAC 3 cut(s) 732, 856, 934
CspAI ACCGGT 1 cut(s) 886
CspCI CAANNNNNGTGG 2 cut(s) 873, 908
CviAII CATG 3 cut(s) 245, 328, 1174
CviQI GTAC 3 cut(s) 732, 856, 934
DdeI CTNAG 4 cut(s) 12, 872, 950, 1053
DinI GGCGCC 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 256
EarI CTCTTC 1 cut(s) 256
Ecl136II GAGCTC 2 cut(s) 505, 1198
Eco24I GRGCYC 4 cut(s) 507, 782, 828, 1200
Eco31I GGTCTC 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 1211
Eco53kI GAGCTC 2 cut(s) 505, 1198
EcoICRI GAGCTC 2 cut(s) 505, 1198
EcoNI CCTNNNNNAGG 1 cut(s) 953
EcoO109I RGGNCCY 1 cut(s) 1211
EcoRII CCWGG 1 cut(s) 591
EcoT38I GRGCYC 4 cut(s) 507, 782, 828, 1200
EgeI GGCGCC 1 cut(s) 340
EheI GGCGCC 1 cut(s) 340
Esp3I CGTCTC 2 cut(s) 663, 708
FaeI CATG 3 cut(s) 248, 331, 1177
FaqI GGGAC 1 cut(s) 649
FatI CATG 3 cut(s) 244, 327, 1173
FauI CCCGC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 151, 453, 456
FokI GGATG 2 cut(s) 266, 1290
FriOI GRGCYC 4 cut(s) 507, 782, 828, 1200
Fsp4HI GCNGC 3 cut(s) 151, 453, 456
FspBI CTAG 4 cut(s) 36, 510, 1007, 1271
GlaI GCGC 1 cut(s) 340
GluI GCNGC 3 cut(s) 151, 453, 456
GsuI CTGGAG 2 cut(s) 229, 1310
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 2 cut(s) 183, 929
HapII CCGG 7 cut(s) 375, 664, 814, 887, 1131, 1157, 1191
HgaI GACGC 2 cut(s) 179, 214
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 4 cut(s) 117, 171, 270, 339
Hin1II CATG 3 cut(s) 248, 331, 1177
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 3 cut(s) 569, 589, 1050
HindII GTYRAC 3 cut(s) 569, 589, 1050
HpaII CCGG 7 cut(s) 375, 664, 814, 887, 1131, 1157, 1191
HphI GGTGA 6 cut(s) 340, 415, 466, 653, 817, 1205
Hpy166II GTNNAC 4 cut(s) 569, 589, 1050, 1183
Hpy188I TCNGA 7 cut(s) 202, 254, 521, 578, 873, 1101, 1238
Hpy188III TCNNGA 8 cut(s) 11, 208, 245, 624, 814, 1068, 1157, 1271
Hpy8I GTNNAC 4 cut(s) 569, 589, 1050, 1183
HpyAV CCTTC 5 cut(s) 172, 205, 763, 1224, 1268
HpyCH4III ACNGT 8 cut(s) 236, 736, 802, 911, 978, 1024, 1064, 1109
HpyCH4IV ACGT 3 cut(s) 117, 270, 681
HpyCH4V TGCA 3 cut(s) 51, 841, 914
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 502, 683
HpyF3I CTNAG 4 cut(s) 12, 872, 950, 1053
HpySE526I ACGT 3 cut(s) 117, 270, 681
Hsp92I GRCGYC 4 cut(s) 117, 171, 270, 339
Hsp92II CATG 3 cut(s) 248, 331, 1177
HspAI GCGC 1 cut(s) 339
KasI GGCGCC 1 cut(s) 338
Kpn2I TCCGGA 2 cut(s) 813, 1156
LmnI GCTCC 5 cut(s) 502, 785, 886, 971, 1070
Lsp1109I GCAGC 1 cut(s) 464
LweI GCATC 3 cut(s) 600, 797, 828
MaeI CTAG 4 cut(s) 36, 510, 1007, 1271
MaeII ACGT 3 cut(s) 117, 270, 681
MaeIII GTNAC 2 cut(s) 118, 346
MboII GAAGA 7 cut(s) 273, 412, 494, 497, 562, 1256, 1324
MflI RGATCY 3 cut(s) 19, 1153, 1293
MhlI GDGCHC 5 cut(s) 507, 782, 828, 976, 1200
MluCI AATT 2 cut(s) 292, 667
Mly113I GGCGCC 1 cut(s) 339
MlyI GAGTC 7 cut(s) 222, 525, 531, 582, 594, 1064, 1105
MmeI TCCRAC 3 cut(s) 232, 434, 1043
MroI TCCGGA 2 cut(s) 813, 1156
MseI TTAA 5 cut(s) 191, 671, 906, 1026, 1266
MslI CAYNNNNRTG 2 cut(s) 605, 1178
MspA1I CMGCKG 1 cut(s) 436
MspI CCGG 7 cut(s) 375, 664, 814, 887, 1131, 1157, 1191
MspR9I CCNGG 2 cut(s) 593, 1191
MvaI CCWGG 1 cut(s) 593
MvnI CGCG 2 cut(s) 150, 225
MwoI GCNNNNNNNGC 3 cut(s) 48, 502, 683
NarI GGCGCC 1 cut(s) 339
NciI CCSGG 1 cut(s) 1191
NlaIII CATG 3 cut(s) 248, 331, 1177
NlaIV GGNNCC 8 cut(s) 154, 340, 441, 628, 781, 882, 1155, 1212
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 2 cut(s) 118, 346
PagI TCATGA 1 cut(s) 244
PcsI WCGNNNNNNNCGW 7 cut(s) 222, 342, 474, 486, 723, 732, 828
PfeI GAWTC 4 cut(s) 808, 819, 868, 1082
PflFI GACNNNGTC 3 cut(s) 272, 584, 1054
PinAI ACCGGT 1 cut(s) 886
PkrI GCNGC 3 cut(s) 152, 454, 457
PleI GAGTC 7 cut(s) 221, 524, 530, 581, 593, 1063, 1104
PluTI GGCGCC 1 cut(s) 342
PpsI GAGTC 7 cut(s) 221, 524, 530, 581, 593, 1063, 1104
PpuMI RGGWCCY 1 cut(s) 1211
PshBI ATTAAT 1 cut(s) 1266
Psp124BI GAGCTC 2 cut(s) 507, 1200
Psp5II RGGWCCY 1 cut(s) 1211
Psp6I CCWGG 1 cut(s) 591
PspGI CCWGG 1 cut(s) 591
PspN4I GGNNCC 8 cut(s) 154, 340, 441, 628, 781, 882, 1155, 1212
PspPI GGNCC 2 cut(s) 181, 1211
PspPPI RGGWCCY 1 cut(s) 1211
PsuI RGATCY 3 cut(s) 19, 1153, 1293
PsyI GACNNNGTC 3 cut(s) 272, 584, 1054
PvuII CAGCTG 1 cut(s) 436
RsaI GTAC 3 cut(s) 733, 857, 935
RsaNI GTAC 3 cut(s) 732, 856, 934
RseI CAYNNNNRTG 2 cut(s) 605, 1178
SacI GAGCTC 2 cut(s) 507, 1200
SaqAI TTAA 5 cut(s) 191, 671, 906, 1026, 1266
SatI GCNGC 3 cut(s) 151, 453, 456
Sau96I GGNCC 2 cut(s) 181, 1211
SchI GAGTC 7 cut(s) 222, 525, 531, 582, 594, 1064, 1105
ScrFI CCNGG 2 cut(s) 593, 1191
SduI GDGCHC 5 cut(s) 507, 782, 828, 976, 1200
SfaNI GCATC 3 cut(s) 600, 797, 828
SfcI CTRYAG 1 cut(s) 70
SfoI GGCGCC 1 cut(s) 340
SinI GGWCC 1 cut(s) 1211
SmiMI CAYNNNNRTG 2 cut(s) 605, 1178
SmlI CTYRAG 2 cut(s) 923, 1205
SmoI CTYRAG 2 cut(s) 923, 1205
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 2 cut(s) 292, 667
SspDI GGCGCC 1 cut(s) 338
SspI AATATT 1 cut(s) 1030
SspMI CTAG 4 cut(s) 36, 510, 1007, 1271
SstI GAGCTC 2 cut(s) 507, 1200
StyD4I CCNGG 2 cut(s) 591, 1189
TaaI ACNGT 8 cut(s) 236, 736, 802, 911, 978, 1024, 1064, 1109
TaiI ACGT 3 cut(s) 120, 273, 684
TaqI TCGA 6 cut(s) 168, 696, 828, 1076, 1307, 1338
TaqII GACCGA 1 cut(s) 597
TasI AATT 2 cut(s) 292, 667
TatI WGTACW 1 cut(s) 855
TauI GCSGC 2 cut(s) 153, 458
TfiI GAWTC 4 cut(s) 808, 819, 868, 1082
Tru1I TTAA 5 cut(s) 191, 671, 906, 1026, 1266
Tru9I TTAA 5 cut(s) 191, 671, 906, 1026, 1266
TseFI GTSAC 2 cut(s) 118, 346
TseI GCWGC 1 cut(s) 452
Tsp45I GTSAC 2 cut(s) 118, 346
TspDTI ATGAA 7 cut(s) 118, 154, 261, 563, 1257, 1272, 1325
TspGWI ACGGA 5 cut(s) 96, 313, 454, 772, 1094
Tth111I GACNNNGTC 3 cut(s) 272, 584, 1054
VpaK11BI GGWCC 1 cut(s) 1211
VspI ATTAAT 1 cut(s) 1266
XagI CCTNNNNNAGG 1 cut(s) 953
XapI RAATTY 2 cut(s) 292, 667
XbaI TCTAGA 1 cut(s) 1270
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XspI CTAG 4 cut(s) 36, 510, 1007, 1271
ZraI GACGTC 2 cut(s) 118, 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.