Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004959

Physical Location & Seq

Genomic Coordinates Forward (+)

384241 .. 387363

Gene Structure 3123 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1004000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGAATCCGTTGGACCAAAAACGAAAACAAGACAATCTCATCCAATTGTCACTGAAATTCAACGGCACGATGGCGAGCAGTCCGACGGTTGATGCCAGAAGCGGCTTCTGCAGTTCCAATTCCGTATTCTACAGCAAGCGCAAGGCCATAACACTACCGCCCAGCGATTATCTCGACATCACAACATTCATATCGTCTCAAGCCCACCGAGGCAATGTGGCCTTCATCGACGGCTCCACCGGCCAACAACTCACCTACGTTCAATTATGGACTGCCGTCGATTCAGTTGCCTCGTCTCTCTCCGACATGGGCATCCGAAAGGGCCATGTCATCCTCCTCCTGTCCCCGAACTCCATCTTCTTCCCGGTGGTCTGCCTGGCCGTGATGTCGCTGGGTGCGATCATAACAACCACCAACCCACTCAACACTACTCGGGAAATCACCAAGCAAATGGCCGATTCCAAACCGGTACTCGCCTTCACTACTCGCCAGCTCATCCCCAAACTCGCCGGTCCAAAACCTCTCCCCATCGTCCTCATTGATGGCGAAATCAATACTCCTGTTAGTAACAAAAATATTGCGTCTACCTTGGGAAAGATGATGGCGAATGAGAAACGTGATGGATTGAAATCGAGGGAAGTGATCAACCAGGACGACACGGCGACTCTGCTCTACTCGTCGGGAACGACGGGGGCGAGTAAAGGCGTGGTGTCGTCTCACAGGAATCTGATAGCGATGGTTCAGAGCGTTAAGGGCTGGTTATATTTGGACGATACCCACCAAACGTTCCTCTGCACGATTCCAATGTTCCACATCTTCGGCTTGGCGGTGTTCGCTGCGGGCCTCCTTTCCTTGGGGTCGACCATCGTGGTGCTCTCCAAGTTCGAGATGCATGACATGCTGTTGGCCATTCAGAAGAACCGTGTGAGCTACGTGCCGGTGGTGCCTCCCATTCTGGTGGCGCTAATCAACGCCGCCGACCAGGTGAAGGCGAAGTATGATCTGAGTTCGCTGAGGTGGGTGTTGTCGGGTGGCGCCCCACTGAGCAAGGAAGTGACCGAGGGGTTCTTGGACAAGTATCCAATGGTGACAATTCTTCAGGGTTACGGGTTGACGGAATCGGCCGGGGTCGGAGCTTCAACGGAATCGCTGGAGGAGAGTCGCAAGTACGGTACGGCGGGGCTGTTGTCGGCGAGTATGGAGGCTATGATTGTGGACCGGGACAGCGGTAATCCACTACCGGCGAATAAGACGGGAGAGCTTTATCTCAAAGGTCCCTCAATAATGAAAGGGTATTTTAATAATGCAAAAGCAACTGCATCAACACTCGATTCAAAAGGATGGTTAAGAACCGGAGATTTGTGCTACATCGATGAGGATGGATTCATTTTTGTGGTTGATAGGTTGAAGGAGCTCATTAAGTACAACGGATATCAGGTGGCCCCTGCTGAACTGGAGGCTTTGCTGCTCACGCATCCCGAAATTGTTGATGCTGCCGTTATACCATTTCCAGATGAAAAGGTTGGACAGCATCCAATGGCATATGTGGTAAGAAAAGCCGGAAGCAGTTTAACGGAGAGCGGAGTGATGGAGTTTGTGGGCAAGCAGGTGGCTCCCTACAAGAGAATTAGGAGGGTGGCATTTATAGATTCCGTGCCTAAGAATCCATCTGGCAAGATTCTCCGGAAGGATCTCATCCAGATTGCAACTTCTTCTTCCAAACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009611 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00592 ko01100 ko01110 map00592 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

576

Amino Acids

62.34

Weight (kDa)

9.0

Isoelectric Point (pI)

37.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	67 - 433	2.9e-86	AMP-binding enzyme
AMP-binding_C	PF13193	484 - 559	9.2e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20480 AT1G20490 AT1G20490 AT1G20500 AT1G20510 AT1G20510 AT1G20510 AT5G38120 AT5G38120 AT5G38120 AT5G38120
fragaria_vesca	FvH4_2g37640 FvH4_2g37640 FvH4_2g37640 FvH4_2g37640
malus_domestica	MD08G1004000.v1.1 MD15G1003500.v1.1
prunus_persica	Prupe.1G355900_v2.0.a1
pyrus_communis	pycom15g00250
rosa_chinensis	RchiOBHm_Chr6g0304091
rosa_laevigata	RLG00000011015
rosa_multiflora	Rmu_sc0003805.1_g000004 Rmu_sc0030558.1_g000003
rosa_roxburghii	Rroxscaffold_7G00164090
rosa_rugosa	Rorug06G0327500
rosa_samantha	Rh6AG441500 Rh6BG461300 Rh6CG453600 Rh6DG440900
rosa_wichuraiana	Rw6G038260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1597
Acc36I	ACCTGC	1 cut(s)	1597
AccB1I	GGYRCC	2 cut(s)	943, 1034
AccBSI	CCGCTC	1 cut(s)	1581
AccI	GTMKAC	2 cut(s)	584, 860
AccIII	TCCGGA	1 cut(s)	1683
AciI	CCGC	8 cut(s)	102, 158, 827, 839, 975, 1178, 1227, 1581
AclI	AACGTT	1 cut(s)	785
AclWI	GGATC	1 cut(s)	1698
AcoI	YGGCCR	5 cut(s)	241, 378, 453, 906, 1122
AcsI	RAATTY	1 cut(s)	56
AcuI	CTGAAG	1 cut(s)	1082
AcyI	GRCGYC	1 cut(s)	1035
AfaI	GTAC	4 cut(s)	471, 1169, 1174, 1424
AfiI	CCNNNNNNNGG	2 cut(s)	853, 988
AgeI	ACCGGT	1 cut(s)	466
AgsI	TTSAA	6 cut(s)	61, 263, 628, 1140, 1335, 1408
AjnI	CCWGG	3 cut(s)	375, 648, 981
AjuI	GAANNNNNNNTTGG	2 cut(s)	110, 142
AluBI	AGCT	5 cut(s)	493, 930, 1136, 1261, 1414
AluI	AGCT	5 cut(s)	493, 930, 1136, 1261, 1414
Alw21I	GWGCWC	2 cut(s)	876, 1416
Alw26I	GTCTC	3 cut(s)	201, 300, 720
AlwI	GGATC	1 cut(s)	1698
Ama87I	CYCGRG	1 cut(s)	432
Aor13HI	TCCGGA	1 cut(s)	1683
ApeKI	GCWGC	3 cut(s)	836, 1465, 1493
ApoI	RAATTY	1 cut(s)	56
AsiGI	ACCGGT	1 cut(s)	466
AspLEI	GCGC	3 cut(s)	141, 964, 1037
AspS9I	GGNCC	7 cut(s)	13, 322, 512, 841, 1216, 1274, 1441
AsuC2I	CCSGG	3 cut(s)	365, 1126, 1220
AsuHPI	GGTGA	4 cut(s)	244, 433, 997, 1099
AvaI	CYCGRG	1 cut(s)	432
AvaII	GGWCC	4 cut(s)	13, 512, 1216, 1274
BalI	TGGCCA	1 cut(s)	908
BanI	GGYRCC	2 cut(s)	943, 1034
BanII	GRGCYC	1 cut(s)	1416
Bbv12I	GWGCWC	2 cut(s)	876, 1416
BbvCI	CCTCAGC	1 cut(s)	1013
BbvI	GCAGC	3 cut(s)	823, 1452, 1480
BceAI	ACGGC	7 cut(s)	79, 247, 260, 365, 675, 1191, 1481
BcgI	CGANNNNNNTGC	2 cut(s)	269, 303
BciT130I	CCWGG	3 cut(s)	377, 650, 983
BciVI	GTATCC	1 cut(s)	1089
BclI	TGATCA	1 cut(s)	642
BcnI	CCSGG	3 cut(s)	365, 1126, 1220
BcoDI	GTCTC	3 cut(s)	201, 300, 720
BfmI	CTRYAG	2 cut(s)	109, 130
BfoI	RGCGCY	2 cut(s)	965, 1038
BfuAI	ACCTGC	1 cut(s)	1597
BfuI	GTATCC	1 cut(s)	1089
BisI	GCNGC	5 cut(s)	103, 837, 975, 1466, 1494
BlsI	GCNGC	5 cut(s)	104, 838, 976, 1467, 1495
Bme1390I	CCNGG	6 cut(s)	365, 377, 650, 983, 1126, 1220
Bme18I	GGWCC	4 cut(s)	13, 512, 1216, 1274
BmeT110I	CYCGRG	1 cut(s)	432
BmgT120I	GGNCC	7 cut(s)	13, 322, 512, 841, 1216, 1274, 1441
BmiI	GGNNCC	6 cut(s)	235, 945, 1036, 1276, 1443, 1614
BmrFI	CCNGG	6 cut(s)	365, 377, 650, 983, 1126, 1220
BmsI	GCATC	7 cut(s)	82, 321, 879, 1328, 1480, 1483, 1540
BoxI	GACNNNNGTC	1 cut(s)	275
BpmI	CTGGAG	2 cut(s)	1172, 1475
Bpu10I	CCTNAGC	1 cut(s)	1013
BpuEI	CTTGAG	1 cut(s)	183
BpuMI	CCSGG	3 cut(s)	365, 1126, 1220
Bsa29I	ATCGAT	1 cut(s)	1371
BsaAI	YACGTR	1 cut(s)	934
BsaBI	GATNNNNATC	1 cut(s)	628
BsaHI	GRCGYC	1 cut(s)	1035
BsaJI	CCNNGG	5 cut(s)	208, 588, 852, 1059, 1125
BsaWI	WCCGGW	3 cut(s)	466, 1352, 1683
Bsc4I	CCNNNNNNNGG	2 cut(s)	853, 988
Bse118I	RCCGGY	5 cut(s)	239, 466, 509, 937, 1240
Bse1I	ACTGG	1 cut(s)	1458
Bse3DI	GCAATG	1 cut(s)	220
Bse8I	GATNNNNATC	1 cut(s)	628
BseAI	TCCGGA	1 cut(s)	1683
BseBI	CCWGG	3 cut(s)	377, 650, 983
BseCI	ATCGAT	1 cut(s)	1371
BseDI	CCNNGG	5 cut(s)	208, 588, 852, 1059, 1125
BseGI	GGATG	9 cut(s)	39, 312, 330, 495, 1346, 1384, 1474, 1531, 1695
BseJI	GATNNNNATC	1 cut(s)	628
BseLI	CCNNNNNNNGG	2 cut(s)	853, 988
BseMI	GCAATG	1 cut(s)	220
BseMII	CTCAG	3 cut(s)	995, 1004, 1034
BseNI	ACTGG	1 cut(s)	1458
BseRI	GAGGAG	2 cut(s)	326, 1169
BseX3I	CGGCCG	1 cut(s)	1122
BseXI	GCAGC	3 cut(s)	823, 1452, 1480
BseYI	CCCAGC	2 cut(s)	161, 391
BsgI	GTGCAG	1 cut(s)	778
Bsh1285I	CGRYCG	1 cut(s)	1125
BshNI	GGYRCC	2 cut(s)	943, 1034
BshTI	ACCGGT	1 cut(s)	466
BshVI	ATCGAT	1 cut(s)	1371
BsiEI	CGRYCG	1 cut(s)	1125
BsiHKAI	GWGCWC	2 cut(s)	876, 1416
BsiHKCI	CYCGRG	1 cut(s)	432
BslFI	GGGAC	3 cut(s)	328, 1235, 1260
BslI	CCNNNNNNNGG	2 cut(s)	853, 988
BsmAI	GTCTC	3 cut(s)	201, 300, 720
BsmBI	CGTCTC	3 cut(s)	201, 300, 720
BsmFI	GGGAC	3 cut(s)	328, 1235, 1260
BsoBI	CYCGRG	1 cut(s)	432
Bsp1286I	GDGCHC	2 cut(s)	876, 1416
Bsp13I	TCCGGA	1 cut(s)	1683
Bsp143I	GATC	4 cut(s)	399, 642, 1000, 1690
BspACI	CCGC	8 cut(s)	102, 158, 827, 839, 975, 1178, 1227, 1581
BspCNI	CTCAG	3 cut(s)	996, 1005, 1035
BspDI	ATCGAT	1 cut(s)	1371
BspEI	TCCGGA	1 cut(s)	1683
BspLI	GGNNCC	6 cut(s)	235, 945, 1036, 1276, 1443, 1614
BspMAI	CTGCAG	1 cut(s)	113
BspMI	ACCTGC	1 cut(s)	1597
BspPI	GGATC	1 cut(s)	1698
BspT107I	GGYRCC	2 cut(s)	943, 1034
BsrBI	CCGCTC	1 cut(s)	1581
BsrDI	GCAATG	1 cut(s)	220
BsrFI	RCCGGY	5 cut(s)	239, 466, 509, 937, 1240
BsrI	ACTGG	1 cut(s)	1458
BssAI	RCCGGY	5 cut(s)	239, 466, 509, 937, 1240
BssECI	CCNNGG	5 cut(s)	208, 588, 852, 1059, 1125
BssMI	GATC	4 cut(s)	399, 642, 1000, 1690
BssNI	GRCGYC	1 cut(s)	1035
BssT1I	CCWWGG	2 cut(s)	588, 852
Bst2UI	CCWGG	3 cut(s)	377, 650, 983
Bst4CI	ACNGT	3 cut(s)	88, 923, 1172
BstACI	GRCGYC	1 cut(s)	1035
BstAPI	GCANNNNNTGC	1 cut(s)	898
BstBAI	YACGTR	1 cut(s)	934
BstC8I	GCNNGC	5 cut(s)	76, 137, 491, 841, 1604
BstDEI	CTNAG	4 cut(s)	1004, 1013, 1043, 1659
BstF5I	GGATG	9 cut(s)	39, 312, 330, 495, 1346, 1384, 1474, 1531, 1695
BstH2I	RGCGCY	2 cut(s)	965, 1038
BstHHI	GCGC	3 cut(s)	141, 964, 1037
BstKTI	GATC	4 cut(s)	402, 645, 1003, 1693
BstMAI	GTCTC	3 cut(s)	201, 300, 720
BstMBI	GATC	4 cut(s)	399, 642, 1000, 1690
BstMCI	CGRYCG	1 cut(s)	1125
BstMWI	GCNNNNNNNGC	7 cut(s)	108, 240, 753, 833, 898, 943, 1471
BstNI	CCWGG	3 cut(s)	377, 650, 983
BstNSI	RCATGY	1 cut(s)	901
BstPAI	GACNNNNGTC	1 cut(s)	275
BstSCI	CCNGG	6 cut(s)	363, 375, 648, 981, 1124, 1218
BstSFI	CTRYAG	2 cut(s)	109, 130
BstV1I	GCAGC	3 cut(s)	823, 1452, 1480
BstX2I	RGATCY	1 cut(s)	1690
BstXI	CCANNNNNNTGG	2 cut(s)	451, 958
BstYI	RGATCY	1 cut(s)	1690
BstZI	CGGCCG	1 cut(s)	1122
Bsu15I	ATCGAT	1 cut(s)	1371
BsuI	GTATCC	1 cut(s)	1089
BsuTUI	ATCGAT	1 cut(s)	1371
BtgZI	GCGATG	1 cut(s)	749
BtsCI	GGATG	9 cut(s)	39, 312, 330, 495, 1346, 1384, 1474, 1531, 1695
BtsIMutI	CAGTG	2 cut(s)	50, 1040
BveI	ACCTGC	1 cut(s)	1597
Cac8I	GCNNGC	5 cut(s)	76, 137, 491, 841, 1604
CfoI	GCGC	3 cut(s)	141, 964, 1037
Cfr10I	RCCGGY	5 cut(s)	239, 466, 509, 937, 1240
Cfr13I	GGNCC	7 cut(s)	13, 322, 512, 841, 1216, 1274, 1441
ClaI	ATCGAT	1 cut(s)	1371
CseI	GACGC	1 cut(s)	570
CsiI	ACCWGGT	1 cut(s)	981
Csp6I	GTAC	4 cut(s)	470, 1168, 1173, 1423
CspAI	ACCGGT	1 cut(s)	466
CviAII	CATG	4 cut(s)	307, 326, 893, 898
CviQI	GTAC	4 cut(s)	470, 1168, 1173, 1423
DdeI	CTNAG	4 cut(s)	1004, 1013, 1043, 1659
DinI	GGCGCC	1 cut(s)	1036
DpnI	GATC	4 cut(s)	401, 644, 1002, 1692
DpnII	GATC	4 cut(s)	399, 642, 1000, 1690
EaeI	YGGCCR	5 cut(s)	241, 378, 453, 906, 1122
EagI	CGGCCG	1 cut(s)	1122
Ecl136II	GAGCTC	1 cut(s)	1414
EclXI	CGGCCG	1 cut(s)	1122
Eco130I	CCWWGG	2 cut(s)	588, 852
Eco24I	GRGCYC	1 cut(s)	1416
Eco32I	GATATC	1 cut(s)	1433
Eco47I	GGWCC	4 cut(s)	13, 512, 1216, 1274
Eco52I	CGGCCG	1 cut(s)	1122
Eco53kI	GAGCTC	1 cut(s)	1414
Eco57I	CTGAAG	1 cut(s)	1082
Eco88I	CYCGRG	1 cut(s)	432
EcoICRI	GAGCTC	1 cut(s)	1414
EcoO109I	RGGNCCY	1 cut(s)	1274
EcoRII	CCWGG	3 cut(s)	375, 648, 981
EcoRV	GATATC	1 cut(s)	1433
EcoT14I	CCWWGG	2 cut(s)	588, 852
EcoT22I	ATGCAT	1 cut(s)	894
EcoT38I	GRGCYC	1 cut(s)	1416
EgeI	GGCGCC	1 cut(s)	1036
EheI	GGCGCC	1 cut(s)	1036
ErhI	CCWWGG	2 cut(s)	588, 852
Esp3I	CGTCTC	3 cut(s)	201, 300, 720
FaeI	CATG	4 cut(s)	310, 329, 896, 901
FaqI	GGGAC	3 cut(s)	328, 1235, 1260
FatI	CATG	4 cut(s)	306, 325, 892, 897
FauI	CCCGC	2 cut(s)	832, 1171
FauNDI	CATATG	1 cut(s)	1543
FbaI	TGATCA	1 cut(s)	642
FblI	GTMKAC	2 cut(s)	584, 860
Fnu4HI	GCNGC	5 cut(s)	103, 837, 975, 1466, 1494
FokI	GGATG	9 cut(s)	26, 299, 317, 482, 1353, 1391, 1461, 1518, 1682
FriOI	GRGCYC	1 cut(s)	1416
Fsp4HI	GCNGC	5 cut(s)	103, 837, 975, 1466, 1494
GlaI	GCGC	3 cut(s)	140, 963, 1036
GluI	GCNGC	5 cut(s)	103, 837, 975, 1466, 1494
GsaI	CCCAGC	2 cut(s)	165, 395
GsuI	CTGGAG	2 cut(s)	1172, 1475
HaeII	RGCGCY	2 cut(s)	965, 1038
HgaI	GACGC	1 cut(s)	570
HhaI	GCGC	3 cut(s)	141, 964, 1037
Hin1I	GRCGYC	1 cut(s)	1035
Hin1II	CATG	4 cut(s)	310, 329, 896, 901
Hin6I	GCGC	3 cut(s)	139, 962, 1035
HinP1I	GCGC	3 cut(s)	139, 962, 1035
HincII	GTYRAC	2 cut(s)	861, 1113
HindII	GTYRAC	2 cut(s)	861, 1113
HphI	GGTGA	4 cut(s)	244, 433, 997, 1099
Hpy166II	GTNNAC	4 cut(s)	585, 861, 1113, 1216
Hpy188I	TCNGA	8 cut(s)	84, 304, 317, 729, 744, 915, 1005, 1133
Hpy188III	TCNNGA	8 cut(s)	173, 434, 681, 886, 1478, 1511, 1684, 1699
Hpy8I	GTNNAC	4 cut(s)	585, 861, 1113, 1216
Hpy99I	CGWCG	5 cut(s)	88, 233, 281, 682, 691
HpyAV	CCTTC	5 cut(s)	232, 487, 982, 1402, 1681
HpyCH4III	ACNGT	3 cut(s)	88, 923, 1172
HpyCH4IV	ACGT	4 cut(s)	258, 616, 785, 933
HpyCH4V	TGCA	6 cut(s)	111, 795, 892, 1307, 1319, 1706
HpyF10VI	GCNNNNNNNGC	7 cut(s)	108, 240, 753, 833, 898, 943, 1471
HpyF3I	CTNAG	4 cut(s)	1004, 1013, 1043, 1659
HpySE526I	ACGT	4 cut(s)	258, 616, 785, 933
Hsp92I	GRCGYC	1 cut(s)	1035
Hsp92II	CATG	4 cut(s)	310, 329, 896, 901
HspAI	GCGC	3 cut(s)	139, 962, 1035
KasI	GGCGCC	1 cut(s)	1034
Kpn2I	TCCGGA	1 cut(s)	1683
Ksp22I	TGATCA	1 cut(s)	642
Kzo9I	GATC	4 cut(s)	399, 642, 1000, 1690
LmnI	GCTCC	4 cut(s)	239, 1133, 1411, 1618
Lsp1109I	GCAGC	3 cut(s)	823, 1452, 1480
LweI	GCATC	7 cut(s)	82, 321, 879, 1328, 1480, 1483, 1540
MabI	ACCWGGT	1 cut(s)	981
MaeII	ACGT	4 cut(s)	258, 616, 785, 933
MaeIII	GTNAC	5 cut(s)	48, 566, 1054, 1087, 1103
MalI	GATC	4 cut(s)	401, 644, 1002, 1692
MbiI	CCGCTC	1 cut(s)	1581
MboI	GATC	4 cut(s)	399, 642, 1000, 1690
MboII	GAAGA	7 cut(s)	349, 352, 808, 928, 1088, 1704, 1707
MfeI	CAATTG	1 cut(s)	44
MflI	RGATCY	1 cut(s)	1690
MhlI	GDGCHC	2 cut(s)	876, 1416
MlsI	TGGCCA	1 cut(s)	908
MluCI	AATT	7 cut(s)	44, 56, 118, 263, 1092, 1482, 1626
MluNI	TGGCCA	1 cut(s)	908
Mly113I	GGCGCC	1 cut(s)	1035
MlyI	GAGTC	2 cut(s)	658, 1168
MmeI	TCCRAC	4 cut(s)	107, 327, 1111, 1504
Mox20I	TGGCCA	1 cut(s)	908
Mph1103I	ATGCAT	1 cut(s)	894
MroI	TCCGGA	1 cut(s)	1683
MscI	TGGCCA	1 cut(s)	908
MseI	TTAA	5 cut(s)	750, 1299, 1346, 1419, 1571
MslI	CAYNNNNRTG	3 cut(s)	869, 956, 1391
Msp20I	TGGCCA	1 cut(s)	908
MspA1I	CMGCKG	1 cut(s)	1227
MspR9I	CCNGG	6 cut(s)	365, 377, 650, 983, 1126, 1220
MunI	CAATTG	1 cut(s)	44
MvaI	CCWGG	3 cut(s)	377, 650, 983
MwoI	GCNNNNNNNGC	7 cut(s)	108, 240, 753, 833, 898, 943, 1471
NarI	GGCGCC	1 cut(s)	1035
NciI	CCSGG	3 cut(s)	365, 1126, 1220
NdeI	CATATG	1 cut(s)	1543
NdeII	GATC	4 cut(s)	399, 642, 1000, 1690
NlaIII	CATG	4 cut(s)	310, 329, 896, 901
NlaIV	GGNNCC	6 cut(s)	235, 945, 1036, 1276, 1443, 1614
NmuCI	GTSAC	3 cut(s)	48, 1054, 1087
NsiI	ATGCAT	1 cut(s)	894
NspI	RCATGY	1 cut(s)	901
PaqCI	CACCTGC	1 cut(s)	1597
PcsI	WCGNNNNNNNCGW	3 cut(s)	395, 683, 692
PinAI	ACCGGT	1 cut(s)	466
PkrI	GCNGC	5 cut(s)	104, 838, 976, 1467, 1495
PleI	GAGTC	2 cut(s)	658, 1167
PluTI	GGCGCC	1 cut(s)	1038
PpsI	GAGTC	2 cut(s)	658, 1167
Ppu21I	YACGTR	1 cut(s)	934
PpuMI	RGGWCCY	1 cut(s)	1274
PshAI	GACNNNNGTC	1 cut(s)	275
Psp124BI	GAGCTC	1 cut(s)	1416
Psp1406I	AACGTT	1 cut(s)	785
Psp5II	RGGWCCY	1 cut(s)	1274
Psp6I	CCWGG	3 cut(s)	375, 648, 981
PspFI	CCCAGC	2 cut(s)	161, 391
PspGI	CCWGG	3 cut(s)	375, 648, 981
PspN4I	GGNNCC	6 cut(s)	235, 945, 1036, 1276, 1443, 1614
PspPI	GGNCC	7 cut(s)	13, 322, 512, 841, 1216, 1274, 1441
PspPPI	RGGWCCY	1 cut(s)	1274
PstI	CTGCAG	1 cut(s)	113
PsuI	RGATCY	1 cut(s)	1690
RsaI	GTAC	4 cut(s)	471, 1169, 1174, 1424
RsaNI	GTAC	4 cut(s)	470, 1168, 1173, 1423
RseI	CAYNNNNRTG	3 cut(s)	869, 956, 1391
SacI	GAGCTC	1 cut(s)	1416
SalI	GTCGAC	1 cut(s)	859
SaqAI	TTAA	5 cut(s)	750, 1299, 1346, 1419, 1571
SatI	GCNGC	5 cut(s)	103, 837, 975, 1466, 1494
Sau3AI	GATC	4 cut(s)	399, 642, 1000, 1690
Sau96I	GGNCC	7 cut(s)	13, 322, 512, 841, 1216, 1274, 1441
SchI	GAGTC	2 cut(s)	658, 1168
ScrFI	CCNGG	6 cut(s)	365, 377, 650, 983, 1126, 1220
SduI	GDGCHC	2 cut(s)	876, 1416
SexAI	ACCWGGT	1 cut(s)	981
SfaNI	GCATC	7 cut(s)	82, 321, 879, 1328, 1480, 1483, 1540
SfcI	CTRYAG	2 cut(s)	109, 130
SfoI	GGCGCC	1 cut(s)	1036
SinI	GGWCC	4 cut(s)	13, 512, 1216, 1274
SmiMI	CAYNNNNRTG	3 cut(s)	869, 956, 1391
SmlI	CTYRAG	1 cut(s)	198
SmoI	CTYRAG	1 cut(s)	198
Sse9I	AATT	7 cut(s)	44, 56, 118, 263, 1092, 1482, 1626
SsiI	CCGC	8 cut(s)	102, 158, 827, 839, 975, 1178, 1227, 1581
SspDI	GGCGCC	1 cut(s)	1034
SspI	AATATT	1 cut(s)	577
SstI	GAGCTC	1 cut(s)	1416
StyD4I	CCNGG	6 cut(s)	363, 375, 648, 981, 1124, 1218
StyI	CCWWGG	2 cut(s)	588, 852
TaaI	ACNGT	3 cut(s)	88, 923, 1172
TaiI	ACGT	4 cut(s)	261, 619, 788, 936
TaqI	TCGA	8 cut(s)	174, 228, 279, 632, 860, 885, 1329, 1371
TaqII	GACCGA	1 cut(s)	1073
TasI	AATT	7 cut(s)	44, 56, 118, 263, 1092, 1482, 1626
TatI	WGTACW	1 cut(s)	1422
TauI	GCSGC	2 cut(s)	105, 977
Tru1I	TTAA	5 cut(s)	750, 1299, 1346, 1419, 1571
Tru9I	TTAA	5 cut(s)	750, 1299, 1346, 1419, 1571
TscAI	CASTG	2 cut(s)	57, 1047
TseFI	GTSAC	3 cut(s)	48, 1054, 1087
TseI	GCWGC	3 cut(s)	836, 1465, 1493
Tsp45I	GTSAC	3 cut(s)	48, 1054, 1087
TspDTI	ATGAA	6 cut(s)	17, 178, 214, 1301, 1375, 1530
TspGWI	ACGGA	6 cut(s)	112, 1130, 1157, 1443, 1589, 1642
TspRI	CASTG	2 cut(s)	57, 1047
VpaK11BI	GGWCC	4 cut(s)	13, 512, 1216, 1274
XapI	RAATTY	1 cut(s)	56
XceI	RCATGY	1 cut(s)	901
XmiI	GTMKAC	2 cut(s)	584, 860
Zsp2I	ATGCAT	1 cut(s)	894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.