Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004959

Physical Location & Seq

Genomic Coordinates Forward (+)

204321 .. 207372

Gene Structure 3052 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1003500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGGCCAACACTCCGACGGTCGACGCCAGAAGCGGCTTCTGCAGTTCCAATTCGGTATTCTACAGCAAGCGCAGGTCCATACCACTCCCGCCCAACGACTCCCTCGACATCACAACCTTCATTTCCTCTCAGGCCCACCGTGGCAACGTGGCCTTCATCGACGGCTCCACCGGCCAACAACTCACCTACGTTCAATTATGGGATGCCGTCCGTTCAGTTGCCTCTTCGCTCTCTGACATGGGCATCCGAAAGGGCCACGTCGTCCTCCTCCTGTCCCCGAACTCCATCTTCTTCCCGGTCGTCTGCCTGGCCGTGATGTCGCTGGGCGCGATCATAACAACCACCAACCCCCTCAACACTACTCGAGAAATCGCCAAGCAAATCGCCGATTCCAAACCGGTGCTCGCCTTCACTACTCGCCAGCTCATCCCCAAACTCGCCGGTCCAATTCCTCTCCCCATCGTCCTCATCAATGACGAAATTGATGCGCCCATTGATAATAAAAGTATTGTGAATACCTTGGGAAAGATGATGGCGATTAAAAAACATGATGGGTTGAAATCGAGGGAAGCAATCATCCAGGACGACACGGCGACGCTGCTTTACTCGTCGGGCACGACGGGGGCGAGTAAAGGCGTGGTGTCGTCTCACAGGAATCTGATAGCAATGGTCCAGACCGTTATCGGCCGGTTCTATTCGGACGATAACCACCACCAAACGTTCCTCTGCACGGTTCCCATGTTCCACATCTATGGTTTGGTGGCGTTCGCGACGGGCCTACTTGCCTCCGGATCGACGATTGTCGTGCTCTCTAAGTTCGAGATGCATGACATGCTTTTGTCCATCCAGAGGAACCGTGTCAGCTATCTGCCGCTGGTGCCTCCCATACTGGTGGCGCTGATTAACGCCGCCGACCAGGTGAAGGCCAAGTACGATCTGAGTTCGTTGAGGTGGGTGTTATCGGGTGGGGCCCCGCTGAGCAAGGAAGTGATCGAGGGGTTCTTGGAGAAGTATCCGATGGTGTCAATTCTTCAGGGTTACGGATTGACGGAATCGACAGGAGTCGGAGCGTCAACGGATTCACTGGAGGAGAGTCGCAAGTACGGTACGGCGGGGCTGTTGTCGGCGAGCATGGAAGCTATGATTGTGGATCCGGACAGCGGTCATCCACTAGCGACGAATAAGACGGGAGAGCTCTGGCTCAAAGGTCCCACGATCATGAAAGGATATTTTAGTAACGCAGAAGCGACTGCATCGACACTTGATTCAAAAGGGTGGTTGAGAACCGGAGACTTGTGCTACATCGATGAGGACGGATTTATTTTTGTAGTAGATAGGTTGAAGGAGCTCATTAAATATAAAGGATATCAGGTGCCCCCTGCGGAACTGGAGGCTTTGCTTCTCACGCACCCTGAAATTGCTGATGCTGCTGTTATACCATTTCCAGATGAAAAGGTTGGACAGTATCCAATGGCATATGTGGTAAGAAATGCCGGAAGCAGTTTAACGGAGAGCGGAGTGATGGAGTTTGTGGGCAAGCAGGTGGCTCCGTACAAGAGAATTAGGAGGGTGGCATTTATAGATTCCGTGCCTAAGAATCCATCTGGCAAGATTCTCAGGAAGGATCTCATTCAGCTTGCAGCTTCTTCTTCTTCTTCTTCTAAACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009611 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00592 ko01100 ko01110 map00592 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

557

Amino Acids

60.16

Weight (kDa)

8.14

Isoelectric Point (pI)

39.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	44 - 411	2.8e-87	AMP-binding enzyme
AMP-binding_C	PF13193	462 - 537	1.9e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20480 AT1G20490 AT1G20490 AT1G20500 AT1G20510 AT1G20510 AT1G20510 AT5G38120 AT5G38120 AT5G38120 AT5G38120
fragaria_vesca	FvH4_2g37640 FvH4_2g37640 FvH4_2g37640 FvH4_2g37640
malus_domestica	MD08G1004000.v1.1 MD15G1003500.v1.1
prunus_persica	Prupe.1G355900_v2.0.a1
pyrus_communis	pycom15g00250
rosa_chinensis	RchiOBHm_Chr6g0304091
rosa_laevigata	RLG00000011015
rosa_multiflora	Rmu_sc0003805.1_g000004 Rmu_sc0030558.1_g000003
rosa_roxburghii	Rroxscaffold_7G00164090
rosa_rugosa	Rorug06G0327500
rosa_samantha	Rh6AG441500 Rh6BG461300 Rh6CG453600 Rh6DG440900
rosa_wichuraiana	Rw6G038260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1531
Acc36I	ACCTGC	2 cut(s)	63, 1531
AccB1I	GGYRCC	2 cut(s)	877, 1372
AccBSI	CCGCTC	1 cut(s)	1515
AccI	GTMKAC	1 cut(s)	21
AccII	CGCG	2 cut(s)	329, 770
AccIII	TCCGGA	2 cut(s)	788, 1153
AciI	CCGC	9 cut(s)	33, 89, 872, 909, 974, 1112, 1161, 1382, 1515
AclI	AACGTT	1 cut(s)	719
AclWI	GGATC	4 cut(s)	799, 1145, 1158, 1632
AcoI	YGGCCR	4 cut(s)	3, 172, 309, 685
AcuI	CTGAAG	1 cut(s)	1016
AcyI	GRCGYC	1 cut(s)	24
AfaI	GTAC	4 cut(s)	932, 1103, 1108, 1553
AfiI	CCNNNNNNNGG	4 cut(s)	730, 849, 922, 1160
AgeI	ACCGGT	1 cut(s)	397
AgsI	TTSAA	4 cut(s)	194, 559, 1269, 1342
AjiI	CACGTC	1 cut(s)	259
AjnI	CCWGG	3 cut(s)	306, 579, 915
AjuI	GAANNNNNNNTTGG	2 cut(s)	41, 73
AluBI	AGCT	7 cut(s)	424, 864, 1139, 1195, 1348, 1636, 1643
AluI	AGCT	7 cut(s)	424, 864, 1139, 1195, 1348, 1636, 1643
Alw21I	GWGCWC	4 cut(s)	405, 810, 1197, 1350
Alw26I	GTCTC	2 cut(s)	651, 1284
AlwI	GGATC	4 cut(s)	799, 1145, 1158, 1632
Ama87I	CYCGRG	1 cut(s)	363
Aor13HI	TCCGGA	2 cut(s)	788, 1153
ApaI	GGGCCC	1 cut(s)	973
ApeKI	GCWGC	3 cut(s)	598, 1427, 1640
AsiGI	ACCGGT	1 cut(s)	397
AspLEI	GCGC	4 cut(s)	72, 329, 490, 898
AspS9I	GGNCC	9 cut(s)	75, 133, 253, 443, 670, 775, 969, 970, 1208
AsuC2I	CCSGG	1 cut(s)	296
AsuHPI	GGTGA	2 cut(s)	175, 931
AvaI	CYCGRG	1 cut(s)	363
AvaII	GGWCC	4 cut(s)	75, 443, 670, 1208
BaeGI	GKGCMC	3 cut(s)	617, 973, 1377
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	1150
BanI	GGYRCC	2 cut(s)	877, 1372
BanII	GRGCYC	3 cut(s)	973, 1197, 1350
BarI	GAAGNNNNNNTAC	2 cut(s)	914, 946
Bbv12I	GWGCWC	4 cut(s)	405, 810, 1197, 1350
BbvI	GCAGC	3 cut(s)	585, 1414, 1652
BccI	CCATC	8 cut(s)	293, 467, 526, 545, 851, 1012, 1516, 1609
BceAI	ACGGC	5 cut(s)	178, 191, 296, 606, 1125
BcgI	CGANNNNNNTGC	4 cut(s)	467, 501, 787, 821
BciT130I	CCWGG	3 cut(s)	308, 581, 917
BciVI	GTATCC	2 cut(s)	1023, 1476
BcnI	CCSGG	1 cut(s)	296
BcoDI	GTCTC	2 cut(s)	651, 1284
BfaI	CTAG	1 cut(s)	1172
BfmI	CTRYAG	2 cut(s)	40, 61
BfoI	RGCGCY	1 cut(s)	899
BfuAI	ACCTGC	2 cut(s)	63, 1531
BfuI	GTATCC	2 cut(s)	1023, 1476
BisI	GCNGC	6 cut(s)	34, 599, 872, 909, 1428, 1641
BlpI	GCTNAGC	1 cut(s)	977
BlsI	GCNGC	6 cut(s)	35, 600, 873, 910, 1429, 1642
Bme1390I	CCNGG	4 cut(s)	296, 308, 581, 917
Bme18I	GGWCC	4 cut(s)	75, 443, 670, 1208
BmeT110I	CYCGRG	1 cut(s)	363
BmgBI	CACGTC	1 cut(s)	259
BmgT120I	GGNCC	9 cut(s)	75, 133, 253, 443, 670, 775, 969, 970, 1208
BmrFI	CCNGG	4 cut(s)	296, 308, 581, 917
BmsI	GCATC	6 cut(s)	193, 252, 475, 813, 1262, 1414
BoxI	GACNNNNGTC	3 cut(s)	800, 1061, 1161
BpmI	CTGGAG	2 cut(s)	1106, 1409
Bpu1102I	GCTNAGC	1 cut(s)	977
BpuMI	CCSGG	1 cut(s)	296
Bsa29I	ATCGAT	1 cut(s)	1305
BsaHI	GRCGYC	1 cut(s)	24
BsaJI	CCNNGG	2 cut(s)	139, 519
BsaWI	WCCGGW	4 cut(s)	397, 788, 1153, 1286
Bsc4I	CCNNNNNNNGG	4 cut(s)	730, 849, 922, 1160
Bse118I	RCCGGY	4 cut(s)	170, 397, 440, 687
Bse1I	ACTGG	3 cut(s)	894, 1089, 1392
Bse3DI	GCAATG	1 cut(s)	672
BseAI	TCCGGA	2 cut(s)	788, 1153
BseBI	CCWGG	3 cut(s)	308, 581, 917
BseCI	ATCGAT	1 cut(s)	1305
BseDI	CCNNGG	2 cut(s)	139, 519
BseGI	GGATG	6 cut(s)	208, 243, 426, 576, 843, 1165
BseLI	CCNNNNNNNGG	4 cut(s)	730, 849, 922, 1160
BseMI	GCAATG	1 cut(s)	672
BseMII	CTCAG	4 cut(s)	143, 929, 968, 1630
BseNI	ACTGG	3 cut(s)	894, 1089, 1392
BseRI	GAGGAG	2 cut(s)	257, 1103
BseSI	GKGCMC	3 cut(s)	617, 973, 1377
BseX3I	CGGCCG	1 cut(s)	685
BseXI	GCAGC	3 cut(s)	585, 1414, 1652
BseYI	CCCAGC	1 cut(s)	322
BsgI	GTGCAG	1 cut(s)	712
Bsh1236I	CGCG	2 cut(s)	329, 770
Bsh1285I	CGRYCG	3 cut(s)	21, 300, 688
BshNI	GGYRCC	2 cut(s)	877, 1372
BshTI	ACCGGT	1 cut(s)	397
BshVI	ATCGAT	1 cut(s)	1305
BsiEI	CGRYCG	3 cut(s)	21, 300, 688
BsiHKAI	GWGCWC	4 cut(s)	405, 810, 1197, 1350
BsiHKCI	CYCGRG	1 cut(s)	363
BsiSI	CCGG	9 cut(s)	171, 296, 398, 441, 688, 789, 1154, 1287, 1494
BslFI	GGGAC	2 cut(s)	259, 1194
BslI	CCNNNNNNNGG	4 cut(s)	730, 849, 922, 1160
BsmAI	GTCTC	2 cut(s)	651, 1284
BsmBI	CGTCTC	1 cut(s)	651
BsmFI	GGGAC	2 cut(s)	259, 1194
BsoBI	CYCGRG	1 cut(s)	363
Bsp120I	GGGCCC	1 cut(s)	969
Bsp1286I	GDGCHC	7 cut(s)	405, 617, 810, 973, 1197, 1350, 1377
Bsp13I	TCCGGA	2 cut(s)	788, 1153
Bsp143I	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
Bsp1720I	GCTNAGC	1 cut(s)	977
Bsp68I	TCGCGA	1 cut(s)	770
BspACI	CCGC	9 cut(s)	33, 89, 872, 909, 974, 1112, 1161, 1382, 1515
BspCNI	CTCAG	4 cut(s)	142, 930, 969, 1629
BspDI	ATCGAT	1 cut(s)	1305
BspEI	TCCGGA	2 cut(s)	788, 1153
BspFNI	CGCG	2 cut(s)	329, 770
BspHI	TCATGA	1 cut(s)	1218
BspMAI	CTGCAG	1 cut(s)	44
BspMI	ACCTGC	2 cut(s)	63, 1531
BspPI	GGATC	4 cut(s)	799, 1145, 1158, 1632
BspT107I	GGYRCC	2 cut(s)	877, 1372
BsrBI	CCGCTC	1 cut(s)	1515
BsrDI	GCAATG	1 cut(s)	672
BsrFI	RCCGGY	4 cut(s)	170, 397, 440, 687
BsrI	ACTGG	3 cut(s)	894, 1089, 1392
BssAI	RCCGGY	4 cut(s)	170, 397, 440, 687
BssECI	CCNNGG	2 cut(s)	139, 519
BssMI	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
BssNI	GRCGYC	1 cut(s)	24
BssT1I	CCWWGG	1 cut(s)	519
Bst2UI	CCWGG	3 cut(s)	308, 581, 917
Bst4CI	ACNGT	7 cut(s)	19, 140, 679, 733, 857, 1106, 1464
Bst6I	CTCTTC	1 cut(s)	229
BstACI	GRCGYC	1 cut(s)	24
BstAPI	GCANNNNNTGC	1 cut(s)	832
BstC8I	GCNNGC	6 cut(s)	68, 405, 422, 1129, 1538, 1638
BstDEI	CTNAG	6 cut(s)	129, 813, 938, 977, 1593, 1616
BstDSI	CCRYGG	1 cut(s)	139
BstF5I	GGATG	6 cut(s)	208, 243, 426, 576, 843, 1165
BstFNI	CGCG	2 cut(s)	329, 770
BstH2I	RGCGCY	1 cut(s)	899
BstHHI	GCGC	4 cut(s)	72, 329, 490, 898
BstKTI	GATC	7 cut(s)	333, 794, 937, 993, 1153, 1218, 1627
BstMAI	GTCTC	2 cut(s)	651, 1284
BstMBI	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
BstMCI	CGRYCG	3 cut(s)	21, 300, 688
BstMWI	GCNNNNNNNGC	6 cut(s)	39, 171, 832, 877, 1405, 1427
BstNI	CCWGG	3 cut(s)	308, 581, 917
BstNSI	RCATGY	1 cut(s)	835
BstPAI	GACNNNNGTC	3 cut(s)	800, 1061, 1161
BstSCI	CCNGG	4 cut(s)	294, 306, 579, 915
BstSFI	CTRYAG	2 cut(s)	40, 61
BstSLI	GKGCMC	3 cut(s)	617, 973, 1377
BstUI	CGCG	2 cut(s)	329, 770
BstV1I	GCAGC	3 cut(s)	585, 1414, 1652
BstX2I	RGATCY	2 cut(s)	1150, 1624
BstXI	CCANNNNNNTGG	2 cut(s)	752, 892
BstYI	RGATCY	2 cut(s)	1150, 1624
BstZI	CGGCCG	1 cut(s)	685
Bsu15I	ATCGAT	1 cut(s)	1305
BsuI	GTATCC	2 cut(s)	1023, 1476
BsuTUI	ATCGAT	1 cut(s)	1305
BtgI	CCRYGG	1 cut(s)	139
BtrI	CACGTC	1 cut(s)	259
BtsCI	GGATG	6 cut(s)	208, 243, 426, 576, 843, 1165
BtsIMutI	CAGTG	1 cut(s)	1082
BtuMI	TCGCGA	1 cut(s)	770
BveI	ACCTGC	2 cut(s)	63, 1531
Cac8I	GCNNGC	6 cut(s)	68, 405, 422, 1129, 1538, 1638
CciI	TCATGA	1 cut(s)	1218
CfoI	GCGC	4 cut(s)	72, 329, 490, 898
Cfr10I	RCCGGY	4 cut(s)	170, 397, 440, 687
Cfr13I	GGNCC	9 cut(s)	75, 133, 253, 443, 670, 775, 969, 970, 1208
ClaI	ATCGAT	1 cut(s)	1305
CseI	GACGC	3 cut(s)	32, 604, 1059
CsiI	ACCWGGT	1 cut(s)	915
Csp6I	GTAC	4 cut(s)	931, 1102, 1107, 1552
CspAI	ACCGGT	1 cut(s)	397
CviAII	CATG	7 cut(s)	238, 548, 739, 827, 832, 1132, 1219
CviQI	GTAC	4 cut(s)	931, 1102, 1107, 1552
DdeI	CTNAG	6 cut(s)	129, 813, 938, 977, 1593, 1616
DpnI	GATC	7 cut(s)	332, 793, 936, 992, 1152, 1217, 1626
DpnII	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
EaeI	YGGCCR	4 cut(s)	3, 172, 309, 685
EagI	CGGCCG	1 cut(s)	685
Eam1104I	CTCTTC	1 cut(s)	229
EarI	CTCTTC	1 cut(s)	229
Ecl136II	GAGCTC	2 cut(s)	1195, 1348
EclXI	CGGCCG	1 cut(s)	685
Eco130I	CCWWGG	1 cut(s)	519
Eco24I	GRGCYC	3 cut(s)	973, 1197, 1350
Eco32I	GATATC	1 cut(s)	1367
Eco47I	GGWCC	4 cut(s)	75, 443, 670, 1208
Eco52I	CGGCCG	1 cut(s)	685
Eco53kI	GAGCTC	2 cut(s)	1195, 1348
Eco57I	CTGAAG	1 cut(s)	1016
Eco88I	CYCGRG	1 cut(s)	363
EcoICRI	GAGCTC	2 cut(s)	1195, 1348
EcoO109I	RGGNCCY	3 cut(s)	969, 970, 1208
EcoRII	CCWGG	3 cut(s)	306, 579, 915
EcoRV	GATATC	1 cut(s)	1367
EcoT14I	CCWWGG	1 cut(s)	519
EcoT22I	ATGCAT	1 cut(s)	828
EcoT38I	GRGCYC	3 cut(s)	973, 1197, 1350
ErhI	CCWWGG	1 cut(s)	519
Esp3I	CGTCTC	1 cut(s)	651
FaeI	CATG	7 cut(s)	241, 551, 742, 830, 835, 1135, 1222
FaqI	GGGAC	2 cut(s)	259, 1194
FatI	CATG	7 cut(s)	237, 547, 738, 826, 831, 1131, 1218
FauI	CCCGC	3 cut(s)	96, 981, 1105
FauNDI	CATATG	1 cut(s)	1477
FblI	GTMKAC	1 cut(s)	21
Fnu4HI	GCNGC	6 cut(s)	34, 599, 872, 909, 1428, 1641
FokI	GGATG	6 cut(s)	215, 230, 413, 563, 830, 1152
FriOI	GRGCYC	3 cut(s)	973, 1197, 1350
Fsp4HI	GCNGC	6 cut(s)	34, 599, 872, 909, 1428, 1641
FspBI	CTAG	1 cut(s)	1172
GlaI	GCGC	4 cut(s)	71, 328, 489, 897
GluI	GCNGC	6 cut(s)	34, 599, 872, 909, 1428, 1641
GsaI	CCCAGC	1 cut(s)	326
GsuI	CTGGAG	2 cut(s)	1106, 1409
HaeII	RGCGCY	1 cut(s)	899
HapII	CCGG	9 cut(s)	171, 296, 398, 441, 688, 789, 1154, 1287, 1494
HgaI	GACGC	3 cut(s)	32, 604, 1059
HhaI	GCGC	4 cut(s)	72, 329, 490, 898
Hin1I	GRCGYC	1 cut(s)	24
Hin1II	CATG	7 cut(s)	241, 551, 742, 830, 835, 1135, 1222
Hin6I	GCGC	4 cut(s)	70, 327, 488, 896
HinP1I	GCGC	4 cut(s)	70, 327, 488, 896
HincII	GTYRAC	2 cut(s)	22, 1074
HindII	GTYRAC	2 cut(s)	22, 1074
HpaII	CCGG	9 cut(s)	171, 296, 398, 441, 688, 789, 1154, 1287, 1494
HphI	GGTGA	2 cut(s)	175, 931
Hpy166II	GTNNAC	2 cut(s)	22, 1074
Hpy188I	TCNGA	8 cut(s)	15, 235, 248, 660, 700, 939, 1017, 1067
Hpy8I	GTNNAC	2 cut(s)	22, 1074
HpyAV	CCTTC	6 cut(s)	127, 163, 418, 916, 1336, 1615
HpyCH4III	ACNGT	7 cut(s)	19, 140, 679, 733, 857, 1106, 1464
HpyCH4IV	ACGT	4 cut(s)	147, 189, 258, 719
HpyCH4V	TGCA	5 cut(s)	42, 729, 826, 1253, 1640
HpyF10VI	GCNNNNNNNGC	6 cut(s)	39, 171, 832, 877, 1405, 1427
HpyF3I	CTNAG	6 cut(s)	129, 813, 938, 977, 1593, 1616
HpySE526I	ACGT	4 cut(s)	147, 189, 258, 719
Hsp92I	GRCGYC	1 cut(s)	24
Hsp92II	CATG	7 cut(s)	241, 551, 742, 830, 835, 1135, 1222
HspAI	GCGC	4 cut(s)	70, 327, 488, 896
Kpn2I	TCCGGA	2 cut(s)	788, 1153
Kzo9I	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
LmnI	GCTCC	4 cut(s)	170, 1067, 1345, 1552
Lsp1109I	GCAGC	3 cut(s)	585, 1414, 1652
LweI	GCATC	6 cut(s)	193, 252, 475, 813, 1262, 1414
MabI	ACCWGGT	1 cut(s)	915
MaeI	CTAG	1 cut(s)	1172
MaeII	ACGT	4 cut(s)	147, 189, 258, 719
MaeIII	GTNAC	2 cut(s)	1037, 1235
MalI	GATC	7 cut(s)	332, 793, 936, 992, 1152, 1217, 1626
MbiI	CCGCTC	1 cut(s)	1515
MboI	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
MboII	GAAGA	9 cut(s)	216, 280, 283, 1022, 1638, 1641, 1644, 1647, 1650
MflI	RGATCY	2 cut(s)	1150, 1624
MhlI	GDGCHC	7 cut(s)	405, 617, 810, 973, 1197, 1350, 1377
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	7 cut(s)	49, 194, 447, 480, 1026, 1416, 1560
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	92, 1071, 1102
MmeI	TCCRAC	3 cut(s)	38, 1045, 1438
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	828
MroI	TCCGGA	2 cut(s)	788, 1153
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	4 cut(s)	540, 903, 1353, 1505
MslI	CAYNNNNRTG	3 cut(s)	750, 890, 1217
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	3 cut(s)	874, 976, 1161
MspI	CCGG	9 cut(s)	171, 296, 398, 441, 688, 789, 1154, 1287, 1494
MspR9I	CCNGG	4 cut(s)	296, 308, 581, 917
MvaI	CCWGG	3 cut(s)	308, 581, 917
MvnI	CGCG	2 cut(s)	329, 770
MwoI	GCNNNNNNNGC	6 cut(s)	39, 171, 832, 877, 1405, 1427
NciI	CCSGG	1 cut(s)	296
NdeI	CATATG	1 cut(s)	1477
NdeII	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
NlaIII	CATG	7 cut(s)	241, 551, 742, 830, 835, 1135, 1222
NruI	TCGCGA	1 cut(s)	770
NsiI	ATGCAT	1 cut(s)	828
NspI	RCATGY	1 cut(s)	835
PaeR7I	CTCGAG	1 cut(s)	363
PagI	TCATGA	1 cut(s)	1218
PaqCI	CACCTGC	1 cut(s)	1531
PcsI	WCGNNNNNNNCGW	4 cut(s)	102, 326, 614, 623
PfeI	GAWTC	8 cut(s)	389, 655, 1052, 1079, 1265, 1583, 1597, 1612
PfoI	TCCNGGA	1 cut(s)	579
PinAI	ACCGGT	1 cut(s)	397
PkrI	GCNGC	6 cut(s)	35, 600, 873, 910, 1429, 1642
PleI	GAGTC	3 cut(s)	92, 1070, 1101
PpsI	GAGTC	3 cut(s)	92, 1070, 1101
PpuMI	RGGWCCY	1 cut(s)	1208
PshAI	GACNNNNGTC	3 cut(s)	800, 1061, 1161
Psp124BI	GAGCTC	2 cut(s)	1197, 1350
Psp1406I	AACGTT	1 cut(s)	719
Psp5II	RGGWCCY	1 cut(s)	1208
Psp6I	CCWGG	3 cut(s)	306, 579, 915
PspFI	CCCAGC	1 cut(s)	322
PspGI	CCWGG	3 cut(s)	306, 579, 915
PspOMI	GGGCCC	1 cut(s)	969
PspPI	GGNCC	9 cut(s)	75, 133, 253, 443, 670, 775, 969, 970, 1208
PspPPI	RGGWCCY	1 cut(s)	1208
PstI	CTGCAG	1 cut(s)	44
PsuI	RGATCY	2 cut(s)	1150, 1624
RruI	TCGCGA	1 cut(s)	770
RsaI	GTAC	4 cut(s)	932, 1103, 1108, 1553
RsaNI	GTAC	4 cut(s)	931, 1102, 1107, 1552
RseI	CAYNNNNRTG	3 cut(s)	750, 890, 1217
SacI	GAGCTC	2 cut(s)	1197, 1350
SalI	GTCGAC	1 cut(s)	20
SaqAI	TTAA	4 cut(s)	540, 903, 1353, 1505
SatI	GCNGC	6 cut(s)	34, 599, 872, 909, 1428, 1641
Sau3AI	GATC	7 cut(s)	330, 791, 934, 990, 1150, 1215, 1624
Sau96I	GGNCC	9 cut(s)	75, 133, 253, 443, 670, 775, 969, 970, 1208
SchI	GAGTC	3 cut(s)	92, 1071, 1102
ScrFI	CCNGG	4 cut(s)	296, 308, 581, 917
SduI	GDGCHC	7 cut(s)	405, 617, 810, 973, 1197, 1350, 1377
SexAI	ACCWGGT	1 cut(s)	915
SfaNI	GCATC	6 cut(s)	193, 252, 475, 813, 1262, 1414
SfcI	CTRYAG	2 cut(s)	40, 61
Sfr274I	CTCGAG	1 cut(s)	363
SinI	GGWCC	4 cut(s)	75, 443, 670, 1208
SlaI	CTCGAG	1 cut(s)	363
SmiMI	CAYNNNNRTG	3 cut(s)	750, 890, 1217
SmlI	CTYRAG	1 cut(s)	363
SmoI	CTYRAG	1 cut(s)	363
Sse9I	AATT	7 cut(s)	49, 194, 447, 480, 1026, 1416, 1560
SsiI	CCGC	9 cut(s)	33, 89, 872, 909, 974, 1112, 1161, 1382, 1515
SspMI	CTAG	1 cut(s)	1172
SstI	GAGCTC	2 cut(s)	1197, 1350
StyD4I	CCNGG	4 cut(s)	294, 306, 579, 915
StyI	CCWWGG	1 cut(s)	519
TaaI	ACNGT	7 cut(s)	19, 140, 679, 733, 857, 1106, 1464
TaiI	ACGT	4 cut(s)	150, 192, 261, 722
TasI	AATT	7 cut(s)	49, 194, 447, 480, 1026, 1416, 1560
TauI	GCSGC	3 cut(s)	36, 874, 911
TfiI	GAWTC	8 cut(s)	389, 655, 1052, 1079, 1265, 1583, 1597, 1612
Tru1I	TTAA	4 cut(s)	540, 903, 1353, 1505
Tru9I	TTAA	4 cut(s)	540, 903, 1353, 1505
TscAI	CASTG	1 cut(s)	1089
TseI	GCWGC	3 cut(s)	598, 1427, 1640
TspDTI	ATGAA	4 cut(s)	109, 145, 1235, 1464
TspGWI	ACGGA	8 cut(s)	200, 1056, 1064, 1091, 1329, 1523, 1539, 1576
TspRI	CASTG	1 cut(s)	1089
VpaK11BI	GGWCC	4 cut(s)	75, 443, 670, 1208
XceI	RCATGY	1 cut(s)	835
XhoI	CTCGAG	1 cut(s)	363
XmiI	GTMKAC	1 cut(s)	21
XspI	CTAG	1 cut(s)	1172
Zsp2I	ATGCAT	1 cut(s)	828

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.