AT1G25240

clathrin assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012835
Physical Location & Seq
Forward (+)
8845142 .. 8846726
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25240.1

Sequence Viewer

Length: 1131 bp
ATGAAGCTGTGGAAACGGGCTTCCGGTGCTCTCAAAGACCGGAAAACCTTGTTTACCATCGGCTTCTCCCGGAAAACCTCCTTCCGCAACCCTGACCTTGATTCTGCCATCATCCACGCAACCTCTCACGATGACTCATCTGTCGATTACCACAATGCTCACCGTGTCTACAAATGGATCCGGTCCTCTCCCGCCAATCTCAAGCCGCTTGTTCATGCTCTTTCATCTAGGGTTAACCGCACAAGAAGCTGGATTGTAGCCTTGAAAGCCTTAATGCTTGTCCACGGTGTGCTTTGCTGCAAAGTCACGTCCCTTCAAGAAATTCGTCGCCTCCCTTTCGATCTCTCGGATTTCTCGGATGGTCATTCCCGTCCCAGCAAAACTTGGGGTTTCAACGCTTTTATCCGCGCCTATTTCTCGTTTCTTGACCAGTACTCTTTCTTTTTATCCGATCAAATCCGTCGTCGCCACAAAAAACCTCAGCTAGATTCGGTTAACCAAGAGCTCGAAAGAATCGAGAAACTTCAGTCTCTTCTCCATATGCTTCTCCAGATCCGTCCAATGGCTGACAACATGAAGAAGACGCTTATCCTTGAAGCCATGGACTGCGTGGTGATCGAGATCTTCGACATTTATGGAAGAATATGTAGCGCTATCGCCAAGCTTCTCATCAAAATCCATCCAGCTGCTGGAAAAGCAGAAGCTGTGATTGCTCTTAAGATTGTAAAGAAGGCTACATCTCAAGGAGAAGATCTCGCACTCTACTTTGAATTCTGCAAAGAATTTGGGGTCTCAAACGCGCACGACATTCCTAAATTCGTGACAATCCCGGAAGAAGACATTAAAGCAATCGAGAAAGTCATCAATGGAGTAGAAGAAGAAGAGGTGAAAAAAAAGGAAGATGAAGTAGAGGAAGAGAAGAGTATCATATTAGTGGAGAGACCGGAGTTGCAGACAATCATAACCGATAAATGGGAAATTTTCGAAGACGACTTTTGTTTCACATGTAAGGATATTAAAGAAACTGATCAGCATAGAAAGTTTAACATGGATCCGAGTCCGCTGCCTCTAATAGTTATCGACGAGCCAGTTTACTTCACTCACACGTTACCAGATTTGATTACCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

376

Amino Acids

43.41

Weight (kDa)

6.9

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 32 - 289 1.2e-76 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012835)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25240 AT1G25240 AT1G68110
fragaria_vesca FvH4_4g26150
malus_domestica MD13G1015400.v1.1 MD16G1012900.v1.1
prunus_persica Prupe.1G338400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433721
rosa_laevigata RLG00000006735
rosa_multiflora Rmu_co8405197.1_g000001 Rmu_sc0000312.1_g000027
rosa_roxburghii Rroxscaffold_5G00375160
rosa_rugosa Rorug04G0271200.1
rosa_samantha Rh4AG325500 Rh4BG333300 Rh4CG348500 Rh4DG329900
rosa_wichuraiana Rw4G028190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 140
AccB7I CCANNNNNTGG 1 cut(s) 689
AccI GTMKAC 1 cut(s) 168
AccII CGCG 2 cut(s) 408, 800
AciI CCGC 6 cut(s) 85, 192, 206, 238, 406, 1061
AclWI GGATC 5 cut(s) 172, 185, 547, 1046, 1059
AcsI RAATTY 5 cut(s) 321, 770, 782, 815, 978
AcuI CTGAAG 1 cut(s) 509
AdeI CACNNNGTG 1 cut(s) 289
AfaI GTAC 1 cut(s) 434
AfeI AGCGCT 1 cut(s) 652
AfiI CCNNNNNNNGG 3 cut(s) 562, 689, 972
AflII CTTAAG 1 cut(s) 716
AflIII ACRYGT 2 cut(s) 1004, 1104
AgsI TTSAA 5 cut(s) 265, 317, 394, 596, 770
AjiI CACGTC 1 cut(s) 309
AluBI AGCT 7 cut(s) 7, 249, 484, 505, 664, 686, 704
AluI AGCT 7 cut(s) 7, 249, 484, 505, 664, 686, 704
Alw21I GWGCWC 2 cut(s) 31, 507
Alw26I GTCTC 3 cut(s) 534, 796, 934
AlwI GGATC 5 cut(s) 172, 185, 547, 1046, 1059
AlwNI CAGNNNCTG 2 cut(s) 689, 704
Aor51HI AGCGCT 1 cut(s) 652
ApeKI GCWGC 3 cut(s) 297, 686, 1063
ApoI RAATTY 5 cut(s) 321, 770, 782, 815, 978
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
AspLEI GCGC 3 cut(s) 410, 653, 802
AspS9I GGNCC 1 cut(s) 183
AsuC2I CCSGG 2 cut(s) 70, 830
AsuHPI GGTGA 3 cut(s) 152, 625, 898
AsuII TTCGAA 1 cut(s) 984
AvaII GGWCC 1 cut(s) 183
BamHI GGATCC 2 cut(s) 177, 1051
BanII GRGCYC 1 cut(s) 507
BbsI GAAGAC 3 cut(s) 587, 843, 993
Bbv12I GWGCWC 2 cut(s) 31, 507
BbvCI CCTCAGC 1 cut(s) 480
BbvI GCAGC 3 cut(s) 284, 673, 1050
BccI CCATC 4 cut(s) 65, 116, 353, 687
BcgI CGANNNNNNTGC 2 cut(s) 1045, 1079
BclI TGATCA 1 cut(s) 1027
BcnI CCSGG 2 cut(s) 70, 830
BcoDI GTCTC 3 cut(s) 534, 796, 934
BfaI CTAG 2 cut(s) 228, 485
BfoI RGCGCY 1 cut(s) 654
BfrI CTTAAG 1 cut(s) 716
BglII AGATCT 2 cut(s) 621, 751
BisI GCNGC 4 cut(s) 206, 298, 687, 1064
BlsI GCNGC 4 cut(s) 207, 299, 688, 1065
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 2 cut(s) 70, 830
Bme18I GGWCC 1 cut(s) 183
BmgBI CACGTC 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 2 cut(s) 179, 1053
BmrFI CCNGG 2 cut(s) 70, 830
BpiI GAAGAC 3 cut(s) 587, 843, 993
BplI GAGNNNNNCTC 2 cut(s) 738, 770
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 480
Bpu14I TTCGAA 1 cut(s) 984
BpuEI CTTGAG 2 cut(s) 185, 726
BpuMI CCSGG 2 cut(s) 70, 830
BsaBI GATNNNNATC 1 cut(s) 620
BsaI GGTCTC 2 cut(s) 796, 934
BsaJI CCNNGG 2 cut(s) 283, 600
BsaWI WCCGGW 4 cut(s) 23, 39, 180, 943
Bsc4I CCNNNNNNNGG 3 cut(s) 562, 689, 972
Bse1I ACTGG 2 cut(s) 430, 1088
Bse8I GATNNNNATC 1 cut(s) 620
BseDI CCNNGG 2 cut(s) 283, 600
BseGI GGATG 3 cut(s) 111, 364, 679
BseJI GATNNNNATC 1 cut(s) 620
BseLI CCNNNNNNNGG 3 cut(s) 562, 689, 972
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 2 cut(s) 430, 1088
BseXI GCAGC 3 cut(s) 284, 673, 1050
BseYI CCCAGC 1 cut(s) 374
Bsh1236I CGCG 2 cut(s) 408, 800
BsiHKAI GWGCWC 2 cut(s) 31, 507
BsiSI CCGG 6 cut(s) 24, 40, 70, 181, 830, 944
BslFI GGGAC 2 cut(s) 295, 357
BslI CCNNNNNNNGG 3 cut(s) 562, 689, 972
BsmAI GTCTC 3 cut(s) 534, 796, 934
BsmFI GGGAC 2 cut(s) 295, 357
Bso31I GGTCTC 2 cut(s) 796, 934
Bsp119I TTCGAA 1 cut(s) 984
Bsp1286I GDGCHC 2 cut(s) 31, 507
Bsp143I GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
Bsp19I CCATGG 1 cut(s) 600
BspACI CCGC 6 cut(s) 85, 192, 206, 238, 406, 1061
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 2 cut(s) 408, 800
BspLI GGNNCC 2 cut(s) 179, 1053
BspPI GGATC 5 cut(s) 172, 185, 547, 1046, 1059
BspT104I TTCGAA 1 cut(s) 984
BspTI CTTAAG 1 cut(s) 716
BspTNI GGTCTC 2 cut(s) 796, 934
BsrI ACTGG 2 cut(s) 430, 1088
BssECI CCNNGG 2 cut(s) 283, 600
BssMI GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
BssT1I CCWWGG 1 cut(s) 600
Bst4CI ACNGT 2 cut(s) 164, 287
Bst6I CTCTTC 4 cut(s) 537, 876, 909, 914
BstAFI CTTAAG 1 cut(s) 716
BstBI TTCGAA 1 cut(s) 984
BstDEI CTNAG 1 cut(s) 480
BstDSI CCRYGG 2 cut(s) 283, 600
BstF5I GGATG 3 cut(s) 111, 364, 679
BstFNI CGCG 2 cut(s) 408, 800
BstH2I RGCGCY 1 cut(s) 654
BstHHI GCGC 3 cut(s) 410, 653, 802
BstKTI GATC 9 cut(s) 180, 343, 454, 555, 618, 624, 754, 1030, 1054
BstMAI GTCTC 3 cut(s) 534, 796, 934
BstMBI GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
BstMWI GCNNNNNNNGC 5 cut(s) 26, 246, 266, 695, 710
BstNSI RCATGY 1 cut(s) 1008
BstSCI CCNGG 2 cut(s) 68, 828
BstUI CGCG 2 cut(s) 408, 800
BstV1I GCAGC 3 cut(s) 284, 673, 1050
BstV2I GAAGAC 3 cut(s) 587, 843, 993
BstX2I RGATCY 5 cut(s) 177, 552, 621, 751, 1051
BstYI RGATCY 5 cut(s) 177, 552, 621, 751, 1051
BtgI CCRYGG 2 cut(s) 283, 600
BtrI CACGTC 1 cut(s) 309
BtsCI GGATG 3 cut(s) 111, 364, 679
CaiI CAGNNNCTG 2 cut(s) 689, 704
CfoI GCGC 3 cut(s) 410, 653, 802
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 1 cut(s) 592
Csp6I GTAC 1 cut(s) 433
CviAII CATG 5 cut(s) 215, 574, 601, 1005, 1048
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 1 cut(s) 480
DpnI GATC 9 cut(s) 179, 342, 453, 554, 617, 623, 753, 1029, 1053
DpnII GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
DraIII CACNNNGTG 1 cut(s) 289
DrdI GACNNNNNNGTC 1 cut(s) 140
DseDI GACNNNNNNGTC 1 cut(s) 140
Eam1104I CTCTTC 4 cut(s) 537, 876, 909, 914
EarI CTCTTC 4 cut(s) 537, 876, 909, 914
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 600
Eco24I GRGCYC 1 cut(s) 507
Eco31I GGTCTC 2 cut(s) 796, 934
Eco47I GGWCC 1 cut(s) 183
Eco47III AGCGCT 1 cut(s) 652
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 770
EcoT14I CCWWGG 1 cut(s) 600
EcoT38I GRGCYC 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 5 cut(s) 218, 577, 604, 1008, 1051
FaqI GGGAC 2 cut(s) 295, 357
FatI CATG 5 cut(s) 214, 573, 600, 1004, 1047
FauI CCCGC 1 cut(s) 199
FauNDI CATATG 1 cut(s) 540
FbaI TGATCA 1 cut(s) 1027
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 4 cut(s) 206, 298, 687, 1064
FokI GGATG 3 cut(s) 98, 371, 666
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 4 cut(s) 206, 298, 687, 1064
FspBI CTAG 2 cut(s) 228, 485
GlaI GCGC 3 cut(s) 409, 652, 801
GluI GCNGC 4 cut(s) 206, 298, 687, 1064
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 533
HaeII RGCGCY 1 cut(s) 654
HapII CCGG 6 cut(s) 24, 40, 70, 181, 830, 944
HgaI GACGC 1 cut(s) 592
HhaI GCGC 3 cut(s) 410, 653, 802
Hin1II CATG 5 cut(s) 218, 577, 604, 1008, 1051
Hin6I GCGC 3 cut(s) 408, 651, 800
HinP1I GCGC 3 cut(s) 408, 651, 800
HincII GTYRAC 2 cut(s) 235, 496
HindII GTYRAC 2 cut(s) 235, 496
HindIII AAGCTT 1 cut(s) 662
HinfI GANTC 5 cut(s) 101, 134, 488, 513, 1057
HpaI GTTAAC 2 cut(s) 235, 496
HpaII CCGG 6 cut(s) 24, 40, 70, 181, 830, 944
HphI GGTGA 3 cut(s) 152, 625, 898
Hpy166II GTNNAC 6 cut(s) 54, 169, 235, 283, 496, 1093
Hpy188I TCNGA 4 cut(s) 349, 358, 451, 1056
Hpy188III TCNNGA 8 cut(s) 128, 317, 425, 517, 550, 619, 820, 853
Hpy8I GTNNAC 6 cut(s) 54, 169, 235, 283, 496, 1093
Hpy99I CGWCG 4 cut(s) 330, 465, 468, 1085
HpyAV CCTTC 3 cut(s) 91, 323, 724
HpyCH4III ACNGT 2 cut(s) 164, 287
HpyCH4IV ACGT 2 cut(s) 308, 1106
HpyCH4V TGCA 3 cut(s) 300, 777, 952
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 246, 266, 695, 710
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 2 cut(s) 308, 1106
Hsp92II CATG 5 cut(s) 218, 577, 604, 1008, 1051
HspAI GCGC 3 cut(s) 408, 651, 800
Ksp22I TGATCA 1 cut(s) 1027
KspAI GTTAAC 2 cut(s) 235, 496
Kzo9I GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
Lsp1109I GCAGC 3 cut(s) 284, 673, 1050
MaeI CTAG 2 cut(s) 228, 485
MaeII ACGT 2 cut(s) 308, 1106
MaeIII GTNAC 3 cut(s) 304, 820, 1107
MalI GATC 9 cut(s) 179, 342, 453, 554, 617, 623, 753, 1029, 1053
MboI GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
MflI RGATCY 5 cut(s) 177, 552, 621, 751, 1051
MhlI GDGCHC 2 cut(s) 31, 507
MluCI AATT 5 cut(s) 321, 770, 782, 815, 978
MlyI GAGTC 2 cut(s) 128, 1066
MnlI CCTC 8 cut(s) 88, 133, 196, 341, 489, 877, 904, 1077
MseI TTAA 7 cut(s) 234, 272, 495, 717, 843, 1017, 1044
MslI CAYNNNNRTG 1 cut(s) 932
MspA1I CMGCKG 2 cut(s) 686, 1063
MspCI CTTAAG 1 cut(s) 716
MspI CCGG 6 cut(s) 24, 40, 70, 181, 830, 944
MspR9I CCNGG 2 cut(s) 70, 830
MvnI CGCG 2 cut(s) 408, 800
MwoI GCNNNNNNNGC 5 cut(s) 26, 246, 266, 695, 710
NciI CCSGG 2 cut(s) 70, 830
NcoI CCATGG 1 cut(s) 600
NdeI CATATG 1 cut(s) 540
NdeII GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
NlaIII CATG 5 cut(s) 218, 577, 604, 1008, 1051
NlaIV GGNNCC 2 cut(s) 179, 1053
NmuCI GTSAC 2 cut(s) 304, 820
NspI RCATGY 1 cut(s) 1008
NspV TTCGAA 1 cut(s) 984
PciI ACATGT 1 cut(s) 1004
PcsI WCGNNNNNNNCGW 2 cut(s) 513, 624
PfeI GAWTC 3 cut(s) 101, 488, 513
PflMI CCANNNNNTGG 1 cut(s) 689
PfoI TCCNGGA 2 cut(s) 68, 828
PkrI GCNGC 4 cut(s) 207, 299, 688, 1065
PleI GAGTC 2 cut(s) 128, 1065
PpsI GAGTC 2 cut(s) 128, 1065
PscI ACATGT 1 cut(s) 1004
Psp124BI GAGCTC 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 374
PspN4I GGNNCC 2 cut(s) 179, 1053
PspPI GGNCC 1 cut(s) 183
PstNI CAGNNNCTG 2 cut(s) 689, 704
PsuI RGATCY 5 cut(s) 177, 552, 621, 751, 1051
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 932
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 7 cut(s) 234, 272, 495, 717, 843, 1017, 1044
SatI GCNGC 4 cut(s) 206, 298, 687, 1064
Sau3AI GATC 9 cut(s) 177, 340, 451, 552, 615, 621, 751, 1027, 1051
Sau96I GGNCC 1 cut(s) 183
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 2 cut(s) 128, 1066
ScrFI CCNGG 2 cut(s) 70, 830
SduI GDGCHC 2 cut(s) 31, 507
SfuI TTCGAA 1 cut(s) 984
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 932
SmlI CTYRAG 3 cut(s) 200, 716, 741
SmoI CTYRAG 3 cut(s) 200, 716, 741
Sse9I AATT 5 cut(s) 321, 770, 782, 815, 978
SsiI CCGC 6 cut(s) 85, 192, 206, 238, 406, 1061
SspMI CTAG 2 cut(s) 228, 485
SstI GAGCTC 1 cut(s) 507
StyD4I CCNGG 2 cut(s) 68, 828
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 2 cut(s) 164, 287
TaiI ACGT 2 cut(s) 311, 1109
TaqI TCGA 9 cut(s) 144, 339, 507, 516, 618, 627, 852, 984, 1080
TasI AATT 5 cut(s) 321, 770, 782, 815, 978
TatI WGTACW 1 cut(s) 432
TauI GCSGC 1 cut(s) 208
TfiI GAWTC 3 cut(s) 101, 488, 513
Tru1I TTAA 7 cut(s) 234, 272, 495, 717, 843, 1017, 1044
Tru9I TTAA 7 cut(s) 234, 272, 495, 717, 843, 1017, 1044
TseFI GTSAC 2 cut(s) 304, 820
TseI GCWGC 3 cut(s) 297, 686, 1063
Tsp45I GTSAC 2 cut(s) 304, 820
TspDTI ATGAA 5 cut(s) 17, 203, 213, 590, 918
TspGWI ACGGA 2 cut(s) 449, 545
Van91I CCANNNNNTGG 1 cut(s) 689
Vha464I CTTAAG 1 cut(s) 716
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 5 cut(s) 321, 770, 782, 815, 978
XceI RCATGY 1 cut(s) 1008
XcmI CCANNNNNNNNNTGG 2 cut(s) 607, 686
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 2 cut(s) 228, 485
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.