MD16G1012900.v1.1

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012835
Physical Location & Seq
Reverse (-)
989425 .. 990693
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1012900.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGAAACTGTGGAAAAAGGCTGCCGGGCTAATCAAAGACCGGAACAGCATCTGGGTGGCCGTCATCTCACGGCGAACGTCGCTCCGCAACCCTGATTTGGAGGCCATCATCATCAAAGCCACCAGCCACGACGAGACCACCGTCGATTACAAGAATTTCCACAGGGTGTACCAATGGGTCCGCACCTCTCCGCTCTACCACAAGCCCCTCCTTTACGCGCTCTCCACGCGCATGGAAAAAACTCGCTCTTGGGTCGTCGCCCTCAAAGGCCTAATGCTCATGCACGGCATTTTCTGCTGCAAAATCCCCTCCGTCCAAAAGATAGGGCGGCTGCCGTTCGACCTCTCCAACTTCTGCGACAAACATTCTAAGCCCAGCAAAACGTGGGCCTACGACGCGTTTGTTCGCGCGTATTTTATGTACTTGGATCAGAGATCCGCGTTTTTTAGCACGTTGGTGAAGAAGAACAACAGAAAATCCGATCAGGAGGAGCCGCCTCTGCTGGAGGAGCTCGGGCGGTTGCAAAAGTGGCAGTCGTTGCTCGATATGCTCCTCGAGATCAAGCCCGAAGCGATCAGTCCGCATCGCACTAATCACACATACAATAACAACAAGGTTTATCTTATTTCCGAAGCAATGATATGTTTACTGACGGAGATCTTCGACGTGTACAGCAGAGTCTCTAAAGGGATTGCCGGAGTGCTGTTGAGGATTTACGCTTCGCCAGGCAAACAGGAGGCGCAGATGGCGCTCGGGGTTGTTCAGAAGGCGGCGGTTCAAGGGGAGGAGCTCTCTTTATACCTCGAATACTGCATGGATATGGGCATCCTCAGCACGGCGGATTGTCCTAAAGTCGAACAGATTCCGGAAGAGGACATCAGAGACCTGGAACGGATAATCAACCAAGCGTCGAAAATGGAGGAAAGCTTCGTGGAATGCGATCGGATGGCAGCGGCTCAGAAAAAGGAGGACAATAAGGCGATCGTGGCCATGCAGAAACCGACGTCGAAGAAATCCAAGACGATAATTACGGACAAATGGGAGGTGTTTGAAGAGGATTTAATCAGCTGGGTGAGCGCAGATACATCAGGTTTCTCGGATGTGAGCGGATACAGCTACAGTAATACGAGCAATAGTGCTATCGGGGTCGGGGTACAAGGACAATATAGAAACCCTTTTGCGCCGTCGTCGAAAAATATAATACCGTATCATCATCCAGTACATGCTGCACATAACCACCAAGTTCTTCCAGATTTGATTAGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

423

Amino Acids

48.14

Weight (kDa)

8.73

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 32 - 301 2.2e-51 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012835)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G25240 AT1G25240 AT1G68110
fragaria_vesca FvH4_4g26150
malus_domestica MD13G1015400.v1.1 MD16G1012900.v1.1
prunus_persica Prupe.1G338400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0433721
rosa_laevigata RLG00000006735
rosa_multiflora Rmu_co8405197.1_g000001 Rmu_sc0000312.1_g000027
rosa_roxburghii Rroxscaffold_5G00375160
rosa_rugosa Rorug04G0271200.1
rosa_samantha Rh4AG325500 Rh4BG333300 Rh4CG348500 Rh4DG329900
rosa_wichuraiana Rw4G028190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1007
AccBSI CCGCTC 2 cut(s) 193, 1107
AccII CGCG 6 cut(s) 218, 229, 398, 408, 410, 440
AccIII TCCGGA 1 cut(s) 865
AclWI GGATC 2 cut(s) 429, 435
AcoI YGGCCR 2 cut(s) 57, 987
AcsI RAATTY 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 1004
AdeI CACNNNGTG 1 cut(s) 166
AfaI GTAC 5 cut(s) 170, 422, 671, 1155, 1221
AfiI CCNNNNNNNGG 2 cut(s) 97, 835
AflIII ACRYGT 2 cut(s) 396, 666
AgsI TTSAA 2 cut(s) 779, 1052
AjiI CACGTC 1 cut(s) 667
AjnI CCWGG 2 cut(s) 724, 885
AleI CACNNNNGTG 1 cut(s) 455
AluBI AGCT 5 cut(s) 511, 790, 927, 1068, 1116
AluI AGCT 5 cut(s) 511, 790, 927, 1068, 1116
Alw21I GWGCWC 2 cut(s) 513, 792
Alw26I GTCTC 3 cut(s) 128, 685, 876
AlwI GGATC 2 cut(s) 429, 435
AlwNI CAGNNNCTG 1 cut(s) 51
Ama87I CYCGRG 3 cut(s) 512, 554, 752
Aor13HI TCCGGA 1 cut(s) 865
AoxI GGCC 5 cut(s) 57, 102, 268, 387, 987
ApeKI GCWGC 5 cut(s) 20, 297, 331, 950, 1226
ApoI RAATTY 1 cut(s) 154
Asp700I GAANNNNTTC 1 cut(s) 861
AspLEI GCGC 7 cut(s) 220, 231, 410, 742, 751, 1079, 1183
AspS9I GGNCC 2 cut(s) 178, 387
AsuC2I CCSGG 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 469, 1084
AvaI CYCGRG 3 cut(s) 512, 554, 752
AvaII GGWCC 1 cut(s) 178
BalI TGGCCA 1 cut(s) 989
BanII GRGCYC 2 cut(s) 513, 792
Bbv12I GWGCWC 2 cut(s) 513, 792
BbvCI CCTCAGC 1 cut(s) 830
BbvI GCAGC 5 cut(s) 7, 284, 318, 962, 1213
BccI CCATC 3 cut(s) 113, 739, 940
BceAI ACGGC 6 cut(s) 44, 86, 301, 319, 852, 1168
BciT130I CCWGG 2 cut(s) 726, 887
BciVI GTATCC 1 cut(s) 1103
BcnI CCSGG 1 cut(s) 25
BcoDI GTCTC 3 cut(s) 128, 685, 876
BfmI CTRYAG 1 cut(s) 1117
BfoI RGCGCY 1 cut(s) 752
BfuI GTATCC 1 cut(s) 1103
BglII AGATCT 1 cut(s) 657
BisI GCNGC 9 cut(s) 21, 298, 329, 332, 494, 771, 951, 954, 1227
BlsI GCNGC 9 cut(s) 22, 299, 330, 333, 495, 772, 952, 955, 1228
Bme1390I CCNGG 3 cut(s) 25, 726, 887
Bme18I GGWCC 1 cut(s) 178
BmeT110I CYCGRG 3 cut(s) 512, 554, 752
BmgBI CACGTC 1 cut(s) 667
BmgT120I GGNCC 2 cut(s) 178, 387
BmiI GGNNCC 2 cut(s) 179, 492
BmrFI CCNGG 3 cut(s) 25, 726, 887
BmsI GCATC 3 cut(s) 57, 592, 834
BoxI GACNNNNGTC 1 cut(s) 140
BplI GAGNNNNNCTC 2 cut(s) 776, 808
BpmI CTGGAG 1 cut(s) 524
Bpu10I CCTNAGC 1 cut(s) 830
BpuMI CCSGG 1 cut(s) 25
BsaHI GRCGYC 1 cut(s) 1004
BsaI GGTCTC 2 cut(s) 128, 876
BsaWI WCCGGW 2 cut(s) 39, 865
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 835
Bse1I ACTGG 1 cut(s) 1217
Bse3DI GCAATG 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 865
BseBI CCWGG 2 cut(s) 726, 887
BseGI GGATG 4 cut(s) 825, 951, 1105, 1213
BseLI CCNNNNNNNGG 2 cut(s) 97, 835
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 2 cut(s) 844, 971
BseNI ACTGG 1 cut(s) 1217
BseRI GAGGAG 4 cut(s) 503, 521, 542, 800
BseXI GCAGC 5 cut(s) 7, 284, 318, 962, 1213
BseYI CCCAGC 2 cut(s) 374, 1068
BsgI GTGCAG 1 cut(s) 1212
Bsh1236I CGCG 6 cut(s) 218, 229, 398, 408, 410, 440
Bsh1285I CGRYCG 2 cut(s) 943, 984
BshFI GGCC 5 cut(s) 59, 104, 270, 389, 989
BsiEI CGRYCG 2 cut(s) 943, 984
BsiHKAI GWGCWC 2 cut(s) 513, 792
BsiHKCI CYCGRG 3 cut(s) 512, 554, 752
BsiSI CCGG 4 cut(s) 24, 40, 696, 866
BslI CCNNNNNNNGG 2 cut(s) 97, 835
BsmAI GTCTC 3 cut(s) 128, 685, 876
BsmI GAATGC 1 cut(s) 941
BsnI GGCC 5 cut(s) 59, 104, 270, 389, 989
Bso31I GGTCTC 2 cut(s) 128, 876
BsoBI CYCGRG 3 cut(s) 512, 554, 752
Bsp1286I GDGCHC 2 cut(s) 513, 792
Bsp13I TCCGGA 1 cut(s) 865
Bsp1407I TGTACA 1 cut(s) 669
Bsp143I GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
BspANI GGCC 5 cut(s) 59, 104, 270, 389, 989
BspCNI CTCAG 2 cut(s) 843, 970
BspEI TCCGGA 1 cut(s) 865
BspFNI CGCG 6 cut(s) 218, 229, 398, 408, 410, 440
BspLI GGNNCC 2 cut(s) 179, 492
BspPI GGATC 2 cut(s) 429, 435
BspTNI GGTCTC 2 cut(s) 128, 876
BsrBI CCGCTC 2 cut(s) 193, 1107
BsrDI GCAATG 1 cut(s) 642
BsrGI TGTACA 1 cut(s) 669
BsrI ACTGG 1 cut(s) 1217
BssMI GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
BssNI GRCGYC 1 cut(s) 1004
Bst2UI CCWGG 2 cut(s) 726, 887
Bst4CI ACNGT 4 cut(s) 9, 142, 1121, 1206
Bst6I CTCTTC 2 cut(s) 864, 1047
BstACI GRCGYC 1 cut(s) 1004
BstAPI GCANNNNNTGC 2 cut(s) 294, 538
BstAUI TGTACA 1 cut(s) 669
BstDEI CTNAG 3 cut(s) 369, 830, 957
BstF5I GGATG 4 cut(s) 825, 951, 1105, 1213
BstFNI CGCG 6 cut(s) 218, 229, 398, 408, 410, 440
BstH2I RGCGCY 1 cut(s) 752
BstHHI GCGC 7 cut(s) 220, 231, 410, 742, 751, 1079, 1183
BstKTI GATC 8 cut(s) 430, 437, 484, 561, 576, 660, 943, 984
BstMAI GTCTC 3 cut(s) 128, 685, 876
BstMBI GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
BstMCI CGRYCG 2 cut(s) 943, 984
BstNI CCWGG 2 cut(s) 726, 887
BstNSI RCATGY 1 cut(s) 1226
BstPAI GACNNNNGTC 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 23, 724, 885
BstSFI CTRYAG 1 cut(s) 1117
BstUI CGCG 6 cut(s) 218, 229, 398, 408, 410, 440
BstV1I GCAGC 5 cut(s) 7, 284, 318, 962, 1213
BstX2I RGATCY 2 cut(s) 434, 657
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 2 cut(s) 434, 657
BsuI GTATCC 1 cut(s) 1103
BsuRI GGCC 5 cut(s) 59, 104, 270, 389, 989
BtgZI GCGATG 1 cut(s) 569
BtrI CACGTC 1 cut(s) 667
BtsCI GGATG 4 cut(s) 825, 951, 1105, 1213
CaiI CAGNNNCTG 1 cut(s) 51
CfoI GCGC 7 cut(s) 220, 231, 410, 742, 751, 1079, 1183
Cfr13I GGNCC 2 cut(s) 178, 387
CseI GACGC 2 cut(s) 404, 897
Csp6I GTAC 5 cut(s) 169, 421, 670, 1154, 1220
CviAII CATG 5 cut(s) 232, 280, 814, 991, 1223
CviQI GTAC 5 cut(s) 169, 421, 670, 1154, 1220
DdeI CTNAG 3 cut(s) 369, 830, 957
DpnI GATC 8 cut(s) 429, 436, 483, 560, 575, 659, 942, 983
DpnII GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
DraIII CACNNNGTG 1 cut(s) 166
EaeI YGGCCR 2 cut(s) 57, 987
Eam1104I CTCTTC 2 cut(s) 864, 1047
EarI CTCTTC 2 cut(s) 864, 1047
EciI GGCGGA 1 cut(s) 854
Ecl136II GAGCTC 2 cut(s) 511, 790
Eco147I AGGCCT 1 cut(s) 270
Eco24I GRGCYC 2 cut(s) 513, 792
Eco31I GGTCTC 2 cut(s) 128, 876
Eco47I GGWCC 1 cut(s) 178
Eco53kI GAGCTC 2 cut(s) 511, 790
Eco88I CYCGRG 3 cut(s) 512, 554, 752
EcoICRI GAGCTC 2 cut(s) 511, 790
EcoRII CCWGG 2 cut(s) 724, 885
EcoT38I GRGCYC 2 cut(s) 513, 792
FaeI CATG 5 cut(s) 235, 283, 817, 994, 1226
FatI CATG 5 cut(s) 231, 279, 813, 990, 1222
Fnu4HI GCNGC 9 cut(s) 21, 298, 329, 332, 494, 771, 951, 954, 1227
FokI GGATG 4 cut(s) 812, 958, 1112, 1200
FriOI GRGCYC 2 cut(s) 513, 792
Fsp4HI GCNGC 9 cut(s) 21, 298, 329, 332, 494, 771, 951, 954, 1227
GlaI GCGC 7 cut(s) 219, 230, 409, 741, 750, 1078, 1182
GluI GCNGC 9 cut(s) 21, 298, 329, 332, 494, 771, 951, 954, 1227
GsaI CCCAGC 2 cut(s) 378, 1072
GsuI CTGGAG 1 cut(s) 524
HaeII RGCGCY 1 cut(s) 752
HaeIII GGCC 5 cut(s) 59, 104, 270, 389, 989
HapII CCGG 4 cut(s) 24, 40, 696, 866
HgaI GACGC 2 cut(s) 404, 897
HhaI GCGC 7 cut(s) 220, 231, 410, 742, 751, 1079, 1183
Hin1I GRCGYC 1 cut(s) 1004
Hin1II CATG 5 cut(s) 235, 283, 817, 994, 1226
Hin6I GCGC 7 cut(s) 218, 229, 408, 740, 749, 1077, 1181
HinP1I GCGC 7 cut(s) 218, 229, 408, 740, 749, 1077, 1181
HindIII AAGCTT 1 cut(s) 925
HinfI GANTC 2 cut(s) 678, 862
HpaII CCGG 4 cut(s) 24, 40, 696, 866
HphI GGTGA 2 cut(s) 469, 1084
Hpy166II GTNNAC 3 cut(s) 169, 647, 670
Hpy188I TCNGA 9 cut(s) 432, 481, 631, 765, 881, 945, 960, 1099, 1268
Hpy188III TCNNGA 4 cut(s) 485, 556, 866, 1250
Hpy8I GTNNAC 3 cut(s) 169, 647, 670
HpyAV CCTTC 1 cut(s) 760
HpyCH4III ACNGT 4 cut(s) 9, 142, 1121, 1206
HpyCH4IV ACGT 5 cut(s) 77, 383, 452, 666, 1004
HpyCH4V TGCA 6 cut(s) 283, 300, 523, 813, 994, 1229
HpyF3I CTNAG 3 cut(s) 369, 830, 957
HpySE526I ACGT 5 cut(s) 77, 383, 452, 666, 1004
Hsp92I GRCGYC 1 cut(s) 1004
Hsp92II CATG 5 cut(s) 235, 283, 817, 994, 1226
HspAI GCGC 7 cut(s) 218, 229, 408, 740, 749, 1077, 1181
Kpn2I TCCGGA 1 cut(s) 865
Kzo9I GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
LmnI GCTCC 5 cut(s) 87, 490, 508, 555, 787
Lsp1109I GCAGC 5 cut(s) 7, 284, 318, 962, 1213
LweI GCATC 3 cut(s) 57, 592, 834
MaeII ACGT 5 cut(s) 77, 383, 452, 666, 1004
MalI GATC 8 cut(s) 429, 436, 483, 560, 575, 659, 942, 983
MbiI CCGCTC 2 cut(s) 193, 1107
MboI GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
MboII GAAGA 7 cut(s) 472, 475, 652, 881, 1021, 1064, 1238
MflI RGATCY 2 cut(s) 434, 657
MhlI GDGCHC 2 cut(s) 513, 792
MlsI TGGCCA 1 cut(s) 989
MluCI AATT 2 cut(s) 154, 1026
MluI ACGCGT 1 cut(s) 396
MluNI TGGCCA 1 cut(s) 989
MlyI GAGTC 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 372
Mox20I TGGCCA 1 cut(s) 989
MroI TCCGGA 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 861
MscI TGGCCA 1 cut(s) 989
MseI TTAA 1 cut(s) 1061
MslI CAYNNNNRTG 4 cut(s) 53, 230, 455, 818
Msp20I TGGCCA 1 cut(s) 989
MspA1I CMGCKG 2 cut(s) 953, 1068
MspI CCGG 4 cut(s) 24, 40, 696, 866
MspR9I CCNGG 3 cut(s) 25, 726, 887
Mva1269I GAATGC 1 cut(s) 941
MvaI CCWGG 2 cut(s) 726, 887
MvnI CGCG 6 cut(s) 218, 229, 398, 408, 410, 440
NciI CCSGG 1 cut(s) 25
NdeII GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
NlaIII CATG 5 cut(s) 235, 283, 817, 994, 1226
NlaIV GGNNCC 2 cut(s) 179, 492
NspI RCATGY 1 cut(s) 1226
OliI CACNNNNGTG 1 cut(s) 455
PaeR7I CTCGAG 1 cut(s) 554
PceI AGGCCT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 936
PctI GAATGC 1 cut(s) 941
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 1 cut(s) 862
PkrI GCNGC 9 cut(s) 22, 299, 330, 333, 495, 772, 952, 955, 1228
Ple19I CGATCG 2 cut(s) 943, 984
PleI GAGTC 1 cut(s) 686
PpsI GAGTC 1 cut(s) 686
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 2 cut(s) 513, 792
Psp6I CCWGG 2 cut(s) 724, 885
PspFI CCCAGC 2 cut(s) 374, 1068
PspGI CCWGG 2 cut(s) 724, 885
PspN4I GGNNCC 2 cut(s) 179, 492
PspPI GGNCC 2 cut(s) 178, 387
PstNI CAGNNNCTG 1 cut(s) 51
PsuI RGATCY 2 cut(s) 434, 657
PvuI CGATCG 2 cut(s) 943, 984
PvuII CAGCTG 1 cut(s) 1068
RsaI GTAC 5 cut(s) 170, 422, 671, 1155, 1221
RsaNI GTAC 5 cut(s) 169, 421, 670, 1154, 1220
RseI CAYNNNNRTG 4 cut(s) 53, 230, 455, 818
SacI GAGCTC 2 cut(s) 513, 792
SaqAI TTAA 1 cut(s) 1061
SatI GCNGC 9 cut(s) 21, 298, 329, 332, 494, 771, 951, 954, 1227
Sau3AI GATC 8 cut(s) 427, 434, 481, 558, 573, 657, 940, 981
Sau96I GGNCC 2 cut(s) 178, 387
SchI GAGTC 1 cut(s) 687
ScrFI CCNGG 3 cut(s) 25, 726, 887
SduI GDGCHC 2 cut(s) 513, 792
SfaNI GCATC 3 cut(s) 57, 592, 834
SfcI CTRYAG 1 cut(s) 1117
Sfr274I CTCGAG 1 cut(s) 554
SinI GGWCC 1 cut(s) 178
SlaI CTCGAG 1 cut(s) 554
SmiMI CAYNNNNRTG 4 cut(s) 53, 230, 455, 818
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 2 cut(s) 154, 1026
SseBI AGGCCT 1 cut(s) 270
SstI GAGCTC 2 cut(s) 513, 792
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 3 cut(s) 23, 724, 885
TaaI ACNGT 4 cut(s) 9, 142, 1121, 1206
TaiI ACGT 5 cut(s) 80, 386, 455, 669, 1007
TasI AATT 2 cut(s) 154, 1026
TatI WGTACW 3 cut(s) 420, 669, 1219
TauI GCSGC 4 cut(s) 331, 496, 773, 956
TfiI GAWTC 1 cut(s) 862
Tru1I TTAA 1 cut(s) 1061
Tru9I TTAA 1 cut(s) 1061
TseI GCWGC 5 cut(s) 20, 297, 331, 950, 1226
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 4 cut(s) 301, 668, 907, 1046
VpaK11BI GGWCC 1 cut(s) 178
XapI RAATTY 1 cut(s) 154
XceI RCATGY 1 cut(s) 1226
XhoI CTCGAG 1 cut(s) 554
XmnI GAANNNNTTC 1 cut(s) 861
ZraI GACGTC 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.