AT1G52560

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014172
Physical Location & Seq
Reverse (-)
19574538 .. 19575866
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G52560.1

Sequence Viewer

Length: 699 bp
ATGGCTCTAGCTCGTCTGGCTTTGAGAAACCTTCAACAAAAACTGTCTCCTAGTTTGATGGGTCAATCTTGTGAAAGAGGTTTGGTCGGAAATCGACATAATCCAATGAAGCTAAACAGATTCATGGCTACTTCGGCTGGTGAACAAGAGGATAAGATGAACACAGAAGTGAGTGTGTCTGAGAAGAAATCTCCTCGACAGAATTTCCCCCGGAGACGTGGCCGAAAAAGCCTCTGGCGTAACACCGACGATCATGGCTACTTCACTCCCACCCTAAATGAGTTCTTTCCTCCGACTATAGGAAATACTCTGATACAAGCAACAGAGAATATGAACAGAATTTTCGACAACTTCAACGTGAATCCATTCCAACTAATGGGTCAAGTGAAAGAGCAAGACGACTGTTACAAGCTCAGATACGAGGTTCCAGGGCTAACCAAAGAAGACGTGAAGATCACGGTAAATGATGGAATCTTGACGATTAAAGGAGATCACAAGGCGGAGGAAGAGAAAGGCTCACCTGAGGAAGACGAGTACTGGTCTTCAAAGAGTTATGGTTACTACAACACGAGCTTATCGTTGCCTGATGATGCTAAGGTTGAGGACATTAAGGCGGAGCTCAAGAATGGTGTGCTTAATCTTGTGATTCCTAGGACGGAGAAGCCAAAGAAGAATGTTCAGGAGATTTCTGTTGAGTAG

Protein Analysis

232

Amino Acids

26.54

Weight (kDa)

6.86

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 129 - 231 2.1e-23 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52560 AT1G52560
fragaria_vesca FvH4_6g21590 FvH4_6g29880
malus_domestica MD17G1209800.v1.1
prunus_persica Prupe.3G085200_v2.0.a1
pyrus_communis pycom17g21520
rosa_chinensis RchiOBHm_Chr2g0135771
rosa_laevigata RLG00000019484
rosa_multiflora Rmu_sc0007848.1_g000021
rosa_roxburghii Rroxscaffold_2G00108840
rosa_rugosa Rorug02G0326300
rosa_samantha Rh2AG378000 Rh2BG384300 Rh2CG363700 Rh2DG400800
rosa_wichuraiana Rw2G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 376
AciI CCGC 2 cut(s) 500, 614
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 2 cut(s) 202, 339
AfaI GTAC 1 cut(s) 536
AfiI CCNNNNNNNGG 2 cut(s) 299, 376
AgsI TTSAA 3 cut(s) 35, 355, 546
AjiI CACGTC 2 cut(s) 218, 448
AjnI CCWGG 1 cut(s) 427
AleI CACNNNNGTG 1 cut(s) 167
AluBI AGCT 5 cut(s) 11, 112, 412, 573, 619
AluI AGCT 5 cut(s) 11, 112, 412, 573, 619
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 2 cut(s) 51, 208
AoxI GGCC 1 cut(s) 220
ApoI RAATTY 2 cut(s) 202, 339
Asp700I GAANNNNTTC 1 cut(s) 365
AspA2I CCTAGG 1 cut(s) 650
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 152, 510
AvrII CCTAGG 1 cut(s) 650
AxyI CCTNAGG 1 cut(s) 522
BanII GRGCYC 1 cut(s) 621
BauI CACGAG 1 cut(s) 568
BbsI GAAGAC 3 cut(s) 450, 534, 534
Bbv12I GWGCWC 1 cut(s) 621
BccI CCATC 2 cut(s) 52, 461
BciT130I CCWGG 1 cut(s) 429
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 2 cut(s) 51, 208
BfaI CTAG 3 cut(s) 8, 51, 651
BfmI CTRYAG 1 cut(s) 297
BlnI CCTAGG 1 cut(s) 650
BmcAI AGTACT 1 cut(s) 536
Bme1390I CCNGG 2 cut(s) 211, 429
BmgBI CACGTC 2 cut(s) 218, 448
BmiI GGNNCC 1 cut(s) 426
BmrFI CCNGG 2 cut(s) 211, 429
BmsI GCATC 1 cut(s) 580
BpiI GAAGAC 3 cut(s) 450, 534, 534
BplI GAGNNNNNCTC 2 cut(s) 500, 532
Bpu10I CCTNAGC 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 211
BsaJI CCNNGG 3 cut(s) 209, 428, 650
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 376
Bse1I ACTGG 1 cut(s) 542
Bse21I CCTNAGG 1 cut(s) 522
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 209, 428, 650
BseLI CCNNNNNNNGG 2 cut(s) 299, 376
BseMII CTCAG 3 cut(s) 171, 427, 513
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 1 cut(s) 211
BslI CCNNNNNNNGG 2 cut(s) 299, 376
BsmAI GTCTC 2 cut(s) 51, 208
BsmBI CGTCTC 1 cut(s) 208
BsnI GGCC 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 3 cut(s) 250, 453, 490
BspACI CCGC 2 cut(s) 500, 614
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 3 cut(s) 172, 426, 514
BspLI GGNNCC 1 cut(s) 426
BsrI ACTGG 1 cut(s) 542
BssECI CCNNGG 3 cut(s) 209, 428, 650
BssMI GATC 3 cut(s) 250, 453, 490
BssSI CACGAG 1 cut(s) 568
BssT1I CCWWGG 1 cut(s) 650
Bst2BI CACGAG 1 cut(s) 568
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 3 cut(s) 45, 404, 460
Bst6I CTCTTC 1 cut(s) 501
BstDEI CTNAG 4 cut(s) 180, 413, 522, 594
BstKTI GATC 3 cut(s) 253, 456, 493
BstMAI GTCTC 2 cut(s) 51, 208
BstMBI GATC 3 cut(s) 250, 453, 490
BstMWI GCNNNNNNNGC 3 cut(s) 17, 134, 228
BstNI CCWGG 1 cut(s) 429
BstSCI CCNGG 2 cut(s) 209, 427
BstSFI CTRYAG 1 cut(s) 297
BstV2I GAAGAC 3 cut(s) 450, 534, 534
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 1 cut(s) 222
BtrI CACGTC 2 cut(s) 218, 448
Csp6I GTAC 1 cut(s) 535
CviAII CATG 2 cut(s) 124, 254
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 4 cut(s) 180, 413, 522, 594
DpnI GATC 3 cut(s) 252, 455, 492
DpnII GATC 3 cut(s) 250, 453, 490
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
EciI GGCGGA 2 cut(s) 515, 629
Ecl136II GAGCTC 1 cut(s) 619
Eco130I CCWWGG 1 cut(s) 650
Eco24I GRGCYC 1 cut(s) 621
Eco53kI GAGCTC 1 cut(s) 619
Eco81I CCTNAGG 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 650
EcoT38I GRGCYC 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 650
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 2 cut(s) 127, 257
FaiI YATR 6 cut(s) 99, 125, 255, 299, 332, 555
FatI CATG 2 cut(s) 123, 253
FriOI GRGCYC 1 cut(s) 621
FspBI CTAG 3 cut(s) 8, 51, 651
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 211
Hin1II CATG 2 cut(s) 127, 257
HinfI GANTC 4 cut(s) 120, 361, 471, 646
HpaII CCGG 1 cut(s) 211
HphI GGTGA 2 cut(s) 152, 510
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 5 cut(s) 89, 181, 294, 312, 416
Hpy188III TCNNGA 3 cut(s) 475, 622, 680
Hpy8I GTNNAC 1 cut(s) 143
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 41
HpyCH4III ACNGT 3 cut(s) 45, 404, 460
HpyCH4IV ACGT 3 cut(s) 217, 357, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 134, 228
HpyF3I CTNAG 4 cut(s) 180, 413, 522, 594
HpySE526I ACGT 3 cut(s) 217, 357, 447
Hsp92II CATG 2 cut(s) 127, 257
Kzo9I GATC 3 cut(s) 250, 453, 490
LmnI GCTCC 1 cut(s) 616
LweI GCATC 1 cut(s) 580
MaeI CTAG 3 cut(s) 8, 51, 651
MaeII ACGT 3 cut(s) 217, 357, 447
MaeIII GTNAC 3 cut(s) 239, 404, 557
MalI GATC 3 cut(s) 252, 455, 492
MboI GATC 3 cut(s) 250, 453, 490
MboII GAAGA 7 cut(s) 196, 455, 463, 518, 534, 539, 682
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 2 cut(s) 202, 339
MmeI TCCRAC 3 cut(s) 67, 317, 394
MnlI CCTC 9 cut(s) 71, 142, 204, 242, 300, 415, 496, 517, 595
MroXI GAANNNNTTC 1 cut(s) 365
MseI TTAA 3 cut(s) 483, 609, 636
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 1 cut(s) 211
MspR9I CCNGG 2 cut(s) 211, 429
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 3 cut(s) 17, 134, 228
NciI CCSGG 1 cut(s) 211
NdeII GATC 3 cut(s) 250, 453, 490
NlaIII CATG 2 cut(s) 127, 257
NlaIV GGNNCC 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 575
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 4 cut(s) 120, 361, 471, 646
PflMI CCANNNNNTGG 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 426
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
RseI CAYNNNNRTG 1 cut(s) 167
SacI GAGCTC 1 cut(s) 621
SaqAI TTAA 3 cut(s) 483, 609, 636
Sau3AI GATC 3 cut(s) 250, 453, 490
ScaI AGTACT 1 cut(s) 536
ScrFI CCNGG 2 cut(s) 211, 429
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 1 cut(s) 580
SfcI CTRYAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 2 cut(s) 202, 339
SsiI CCGC 2 cut(s) 500, 614
SspMI CTAG 3 cut(s) 8, 51, 651
SstI GAGCTC 1 cut(s) 621
StyD4I CCNGG 2 cut(s) 209, 427
StyI CCWWGG 1 cut(s) 650
TaaI ACNGT 3 cut(s) 45, 404, 460
TaiI ACGT 3 cut(s) 220, 360, 450
TaqI TCGA 3 cut(s) 94, 196, 345
TasI AATT 2 cut(s) 202, 339
TatI WGTACW 1 cut(s) 534
TfiI GAWTC 4 cut(s) 120, 361, 471, 646
Tru1I TTAA 3 cut(s) 483, 609, 636
Tru9I TTAA 3 cut(s) 483, 609, 636
TspDTI ATGAA 4 cut(s) 112, 122, 173, 347
TspGWI ACGGA 1 cut(s) 671
Van91I CCANNNNNTGG 1 cut(s) 376
XapI RAATTY 2 cut(s) 202, 339
XmaJI CCTAGG 1 cut(s) 650
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 3 cut(s) 8, 51, 651
ZrmI AGTACT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.