MD17G1209800.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014172
Physical Location & Seq
Forward (+)
25460073 .. 25461508
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1209800.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGCTTTGGCACGATTGGCGTTGAAGAAGTTGCACCAGAGCCAAAGGGTGCTTTCTCCACCACCTTTTTCTGCTGCTTCTTCTCTTCTAGGTCACGGTGTTGGTGAGAGGACAGTTGGAGAGTGCAGAGGCAAAGTTGGCAATGAGATTGCGAAGAGGTTTTCCACGGCAGCTAACGACAAGGCATCCGATGAGAAATCGGAGAACAAAGATGTCGCTGTGTCTCAGGGCAAAAGGTCGAGGTTGTTCCCTAGAAGGCAACGCAGAAGAGGGGGCCTTTGGAGGGATAATGACAGAGACTTCGCCCCTTCGCTTTATGAATTCTTCCCCTCAGGCCTTGGAAATGCACTTGTACAAGCAACTGAGAACATAAACAGGCTGCTTGACAACCTAAACATATCACCATGGTCGCTGTCCGGGCGTGTCAAAGAGCAAAAAGATAGCTACAAACTGCAGTATGATGTGCCAGGGCTTGCCAAGGAGCACGTGAAGATCATTGTTCATGACGGGTTTCTGGAAATCAAGGGAGAGCACAAAGACGAGGACGAGGAAGGGTCGGATGATGAGTATTGGTCATCAAGGAGCTATGGTTACTATAACACAACTCTCAGTTTGCCTGATGATGCCAAAGTTGATGATATAAAGGCAGAGTTGAAGGATGGGGTACTGAGCATAACCATTCCTAAAACTGAGAAGCCTAAGAAGGAGGTGAAGGAGGTCAATATAAATTGA

Protein Analysis

244

Amino Acids

27.26

Weight (kDa)

8.45

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 142 - 243 1.1e-26 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52560 AT1G52560
fragaria_vesca FvH4_6g21590 FvH4_6g29880
malus_domestica MD17G1209800.v1.1
prunus_persica Prupe.3G085200_v2.0.a1
pyrus_communis pycom17g21520
rosa_chinensis RchiOBHm_Chr2g0135771
rosa_laevigata RLG00000019484
rosa_multiflora Rmu_sc0007848.1_g000021
rosa_roxburghii Rroxscaffold_2G00108840
rosa_rugosa Rorug02G0326300
rosa_samantha Rh2AG378000 Rh2BG384300 Rh2CG363700 Rh2DG400800
rosa_wichuraiana Rw2G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 320
AcvI CACGTG 1 cut(s) 487
AfaI GTAC 2 cut(s) 354, 666
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 2 cut(s) 25, 655
AjnI CCWGG 1 cut(s) 466
AluBI AGCT 3 cut(s) 173, 444, 585
AluI AGCT 3 cut(s) 173, 444, 585
Alw21I GWGCWC 2 cut(s) 486, 534
Alw26I GTCTC 2 cut(s) 228, 291
AoxI GGCC 2 cut(s) 274, 334
ApeKI GCWGC 3 cut(s) 74, 170, 379
ApoI RAATTY 1 cut(s) 320
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AspS9I GGNCC 1 cut(s) 274
AsuC2I CCSGG 1 cut(s) 418
AsuHPI GGTGA 3 cut(s) 116, 393, 721
AxyI CCTNAGG 1 cut(s) 331
BbrPI CACGTG 1 cut(s) 487
Bbv12I GWGCWC 2 cut(s) 486, 534
BbvI GCAGC 3 cut(s) 61, 182, 366
BccI CCATC 1 cut(s) 653
BceAI ACGGC 1 cut(s) 183
BciT130I CCWGG 1 cut(s) 468
BcnI CCSGG 1 cut(s) 418
BcoDI GTCTC 2 cut(s) 228, 291
BfaI CTAG 2 cut(s) 89, 252
BfmI CTRYAG 1 cut(s) 452
BisI GCNGC 3 cut(s) 75, 171, 380
BlsI GCNGC 3 cut(s) 76, 172, 381
Bme1390I CCNGG 2 cut(s) 418, 468
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 2 cut(s) 418, 468
BmsI GCATC 2 cut(s) 194, 613
BpuMI CCSGG 1 cut(s) 418
BsaAI YACGTR 1 cut(s) 487
BsaJI CCNNGG 5 cut(s) 165, 337, 404, 467, 477
BsaXI ACNNNNNCTCC 2 cut(s) 574, 604
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse21I CCTNAGG 1 cut(s) 331
Bse3DI GCAATG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 5 cut(s) 165, 337, 404, 467, 477
BseGI GGATG 3 cut(s) 185, 565, 664
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 148
BseMII CTCAG 6 cut(s) 239, 345, 354, 622, 659, 681
BseXI GCAGC 3 cut(s) 61, 182, 366
BsgI GTGCAG 1 cut(s) 145
BshFI GGCC 2 cut(s) 276, 336
BsiHKAI GWGCWC 2 cut(s) 486, 534
BsiSI CCGG 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 2 cut(s) 228, 291
BsnI GGCC 2 cut(s) 276, 336
Bsp1286I GDGCHC 2 cut(s) 486, 534
Bsp1407I TGTACA 1 cut(s) 352
Bsp143I GATC 1 cut(s) 492
Bsp19I CCATGG 1 cut(s) 404
BspANI GGCC 2 cut(s) 276, 336
BspCNI CTCAG 6 cut(s) 238, 344, 355, 621, 660, 682
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 275
BspMAI CTGCAG 1 cut(s) 456
BsrDI GCAATG 1 cut(s) 148
BsrGI TGTACA 1 cut(s) 352
BssECI CCNNGG 5 cut(s) 165, 337, 404, 467, 477
BssMI GATC 1 cut(s) 492
BssT1I CCWWGG 3 cut(s) 337, 404, 477
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 2 cut(s) 98, 115
Bst6I CTCTTC 3 cut(s) 90, 149, 262
BstAUI TGTACA 1 cut(s) 352
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 7 cut(s) 225, 331, 363, 608, 668, 690, 699
BstDSI CCRYGG 2 cut(s) 165, 404
BstF5I GGATG 3 cut(s) 185, 565, 664
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 2 cut(s) 228, 291
BstMBI GATC 1 cut(s) 492
BstMWI GCNNNNNNNGC 3 cut(s) 17, 138, 418
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 416, 466
BstSFI CTRYAG 1 cut(s) 452
BstV1I GCAGC 3 cut(s) 61, 182, 366
Bsu36I CCTNAGG 1 cut(s) 331
BsuRI GGCC 2 cut(s) 276, 336
BtgI CCRYGG 2 cut(s) 165, 404
BtsCI GGATG 3 cut(s) 185, 565, 664
Cac8I GCNNGC 1 cut(s) 474
CciI TCATGA 1 cut(s) 502
Cfr13I GGNCC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 353, 665
CviAII CATG 2 cut(s) 405, 503
CviQI GTAC 2 cut(s) 353, 665
DdeI CTNAG 7 cut(s) 225, 331, 363, 608, 668, 690, 699
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
Eam1104I CTCTTC 3 cut(s) 90, 149, 262
EarI CTCTTC 3 cut(s) 90, 149, 262
Eco130I CCWWGG 3 cut(s) 337, 404, 477
Eco147I AGGCCT 1 cut(s) 336
Eco72I CACGTG 1 cut(s) 487
Eco81I CCTNAGG 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 274
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 466
EcoT14I CCWWGG 3 cut(s) 337, 404, 477
ErhI CCWWGG 3 cut(s) 337, 404, 477
FaeI CATG 2 cut(s) 408, 506
FatI CATG 2 cut(s) 404, 502
Fnu4HI GCNGC 3 cut(s) 75, 171, 380
FokI GGATG 3 cut(s) 172, 572, 671
Fsp4HI GCNGC 3 cut(s) 75, 171, 380
FspBI CTAG 2 cut(s) 89, 252
GluI GCNGC 3 cut(s) 75, 171, 380
HaeIII GGCC 2 cut(s) 276, 336
HapII CCGG 1 cut(s) 417
Hin1II CATG 2 cut(s) 408, 506
HpaII CCGG 1 cut(s) 417
HphI GGTGA 3 cut(s) 116, 393, 721
Hpy188I TCNGA 3 cut(s) 190, 202, 559
Hpy188III TCNNGA 2 cut(s) 503, 515
HpyAV CCTTC 6 cut(s) 249, 318, 545, 649, 697, 706
HpyCH4III ACNGT 2 cut(s) 98, 115
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 4 cut(s) 34, 126, 347, 454
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 138, 418
HpyF3I CTNAG 7 cut(s) 225, 331, 363, 608, 668, 690, 699
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 2 cut(s) 408, 506
Kzo9I GATC 1 cut(s) 492
LmnI GCTCC 2 cut(s) 481, 582
LpnPI CCDG 9 cut(s) 50, 212, 318, 361, 430, 453, 480, 500, 630
Lsp1109I GCAGC 3 cut(s) 61, 182, 366
LweI GCATC 2 cut(s) 194, 613
MaeI CTAG 2 cut(s) 89, 252
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 92, 590
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 7 cut(s) 37, 72, 77, 166, 279, 316, 502
MhlI GDGCHC 2 cut(s) 486, 534
MluCI AATT 2 cut(s) 320, 727
MmeI TCCRAC 2 cut(s) 97, 537
MspI CCGG 1 cut(s) 417
MspR9I CCNGG 2 cut(s) 418, 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 3 cut(s) 17, 138, 418
NciI CCSGG 1 cut(s) 418
NcoI CCATGG 1 cut(s) 404
NdeII GATC 1 cut(s) 492
NlaIII CATG 2 cut(s) 408, 506
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 1 cut(s) 92
PagI TCATGA 1 cut(s) 502
PceI AGGCCT 1 cut(s) 336
PkrI GCNGC 3 cut(s) 76, 172, 381
PmaCI CACGTG 1 cut(s) 487
PmlI CACGTG 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 466
PspCI CACGTG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 1 cut(s) 274
PstI CTGCAG 1 cut(s) 456
RsaI GTAC 2 cut(s) 354, 666
RsaNI GTAC 2 cut(s) 353, 665
SatI GCNGC 3 cut(s) 75, 171, 380
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 1 cut(s) 274
ScrFI CCNGG 2 cut(s) 418, 468
SduI GDGCHC 2 cut(s) 486, 534
SfaNI GCATC 2 cut(s) 194, 613
SfcI CTRYAG 1 cut(s) 452
Sse9I AATT 2 cut(s) 320, 727
SseBI AGGCCT 1 cut(s) 336
SspMI CTAG 2 cut(s) 89, 252
StuI AGGCCT 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 416, 466
StyI CCWWGG 3 cut(s) 337, 404, 477
TaaI ACNGT 2 cut(s) 98, 115
TaiI ACGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 239
TasI AATT 2 cut(s) 320, 727
TatI WGTACW 1 cut(s) 352
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 3 cut(s) 74, 170, 379
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 2 cut(s) 333, 491
XapI RAATTY 1 cut(s) 320
XspI CTAG 2 cut(s) 89, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.