Rroxscaffold_2G00108840

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014172
Physical Location & Seq
Forward (+)
32946373 .. 32947763
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108840.1

Sequence Viewer

Length: 684 bp
ATGGCTTTGGCACGTTTGGCTTTGAAGAAGCACTTTCACCAGAGGGTGCTTTCTCCATCTTCTTCTTTCTCTGCCGCTAACTCTGTGTTATTGGGTCGTGGTGGTAGTGACAGGAGGTTGCTGGCTACAGACTCTAGTGATAAGGTGACTCGTGAGAAAACAACCGACGACAAAGATCTTAGCATCTCCGAGGCTAAAGGTAGATGGACCAACTTGTTCCCTAACTGGAGCCGCAGTAGGGGATTGTGGAGCAATAGGGATCGAGACTTTGTTCCTGCCCTTTCTGGCCTTGAAAGAGCGTTGTTACACGCAACCGGGAACATAAGTAGGTTGTTTGAGAACCTAAACATATCACCATGGTCAGTATCCGGGCGTGTCAAAGAGCAAGACGACTGCTACAAATTGCGGTTTGACATGCCTGGACTTACCAAGGAGGATGTGAAGATTAGTGTTCATCACGGAGTTCTGACAATCAAGGGGGAGCACAAGGACGAAGAGGGAGAAGAGACGGAGGACGAATTTTGGTCATCAAGGAGTTATGGTTATTATCACACTAGTTTCGCGTTGCCTGATGATGCTAAAGTTGATGAGATAAAGGCATCGTTGAGGGATGGGGTGCTGTCTGTTACCATTCCTAGAACTGAGAAGCCTGCGCAAGATGTGAAGGAGGTCAATGTACACTGA

Protein Analysis

227

Amino Acids

25.58

Weight (kDa)

6.72

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 126 - 226 2.7e-27 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52560 AT1G52560
fragaria_vesca FvH4_6g21590 FvH4_6g29880
malus_domestica MD17G1209800.v1.1
prunus_persica Prupe.3G085200_v2.0.a1
pyrus_communis pycom17g21520
rosa_chinensis RchiOBHm_Chr2g0135771
rosa_laevigata RLG00000019484
rosa_multiflora Rmu_sc0007848.1_g000021
rosa_roxburghii Rroxscaffold_2G00108840
rosa_rugosa Rorug02G0326300
rosa_samantha Rh2AG378000 Rh2BG384300 Rh2CG363700 Rh2DG400800
rosa_wichuraiana Rw2G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 654
AccII CGCG 1 cut(s) 563
AciI CCGC 3 cut(s) 75, 232, 406
AclWI GGATC 1 cut(s) 267
AcsI RAATTY 1 cut(s) 518
AfaI GTAC 1 cut(s) 678
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 2 cut(s) 25, 293
AhlI ACTAGT 1 cut(s) 554
AjnI CCWGG 1 cut(s) 418
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 2 cut(s) 258, 500
AlwI GGATC 1 cut(s) 267
AoxI GGCC 1 cut(s) 286
ApoI RAATTY 1 cut(s) 518
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 1 cut(s) 207
AsuC2I CCSGG 2 cut(s) 316, 370
AsuHPI GGTGA 3 cut(s) 29, 157, 345
AvaII GGWCC 1 cut(s) 207
BauI CACGAG 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 486
BccI CCATC 3 cut(s) 64, 198, 605
BciT130I CCWGG 1 cut(s) 420
BciVI GTATCC 1 cut(s) 376
BcnI CCSGG 2 cut(s) 316, 370
BcoDI GTCTC 2 cut(s) 258, 500
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 3 cut(s) 135, 555, 636
BfmI CTRYAG 1 cut(s) 126
BfuI GTATCC 1 cut(s) 376
BglII AGATCT 1 cut(s) 175
BisI GCNGC 2 cut(s) 75, 232
BlsI GCNGC 2 cut(s) 76, 233
Bme1390I CCNGG 3 cut(s) 316, 370, 420
Bme18I GGWCC 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 1 cut(s) 230
BmrFI CCNGG 3 cut(s) 316, 370, 420
BmsI GCATC 3 cut(s) 192, 565, 608
BpmI CTGGAG 1 cut(s) 247
BpuMI CCSGG 2 cut(s) 316, 370
BsaJI CCNNGG 3 cut(s) 189, 356, 429
BsaXI ACNNNNNCTCC 4 cut(s) 220, 250, 526, 556
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse1I ACTGG 1 cut(s) 230
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 3 cut(s) 189, 356, 429
BseGI GGATG 2 cut(s) 442, 616
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 563
BshFI GGCC 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 2 cut(s) 315, 369
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 2 cut(s) 258, 500
BsmBI CGTCTC 1 cut(s) 500
BsnI GGCC 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 486
Bsp1407I TGTACA 1 cut(s) 676
Bsp143I GATC 2 cut(s) 175, 259
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 3 cut(s) 75, 232, 406
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 1 cut(s) 634
BspFNI CGCG 1 cut(s) 563
BspLI GGNNCC 1 cut(s) 230
BspPI GGATC 1 cut(s) 267
BsrGI TGTACA 1 cut(s) 676
BsrI ACTGG 1 cut(s) 230
BssECI CCNNGG 3 cut(s) 189, 356, 429
BssMI GATC 2 cut(s) 175, 259
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 356, 429
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 420
Bst6I CTCTTC 2 cut(s) 489, 498
BstAUI TGTACA 1 cut(s) 676
BstC8I GCNNGC 2 cut(s) 123, 651
BstDEI CTNAG 2 cut(s) 179, 642
BstDSI CCRYGG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 442, 616
BstFNI CGCG 1 cut(s) 563
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 2 cut(s) 178, 262
BstMAI GTCTC 2 cut(s) 258, 500
BstMBI GATC 2 cut(s) 175, 259
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 3 cut(s) 314, 368, 418
BstSFI CTRYAG 1 cut(s) 126
BstUI CGCG 1 cut(s) 563
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 2 cut(s) 442, 616
BtsIMutI CAGTG 1 cut(s) 679
Cac8I GCNNGC 2 cut(s) 123, 651
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 1 cut(s) 207
Csp6I GTAC 1 cut(s) 677
CviAII CATG 2 cut(s) 357, 415
CviJI RGCY 7 cut(s) 5, 20, 125, 194, 231, 288, 649
CviKI_1 RGCY 7 cut(s) 5, 20, 125, 194, 231, 288, 649
CviQI GTAC 1 cut(s) 677
DdeI CTNAG 2 cut(s) 179, 642
DpnI GATC 2 cut(s) 177, 261
DpnII GATC 2 cut(s) 175, 259
Eam1104I CTCTTC 2 cut(s) 489, 498
EarI CTCTTC 2 cut(s) 489, 498
Eco130I CCWWGG 2 cut(s) 356, 429
Eco47I GGWCC 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 2 cut(s) 356, 429
ErhI CCWWGG 2 cut(s) 356, 429
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 2 cut(s) 360, 418
FaiI YATR 5 cut(s) 323, 350, 358, 416, 540
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FatI CATG 2 cut(s) 356, 414
Fnu4HI GCNGC 2 cut(s) 75, 232
FokI GGATG 2 cut(s) 449, 623
Fsp4HI GCNGC 2 cut(s) 75, 232
FspBI CTAG 3 cut(s) 135, 555, 636
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 1 cut(s) 654
GluI GCNGC 2 cut(s) 75, 232
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 1 cut(s) 288
HapII CCGG 2 cut(s) 315, 369
HhaI GCGC 1 cut(s) 655
Hin1II CATG 2 cut(s) 360, 418
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HinfI GANTC 2 cut(s) 131, 148
HpaII CCGG 2 cut(s) 315, 369
HphI GGTGA 3 cut(s) 29, 157, 345
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 2 cut(s) 190, 468
Hpy188III TCNNGA 2 cut(s) 152, 263
Hpy8I GTNNAC 1 cut(s) 679
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 658
HpyCH4IV ACGT 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpyF3I CTNAG 2 cut(s) 179, 642
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 2 cut(s) 360, 418
HspAI GCGC 1 cut(s) 653
Kzo9I GATC 2 cut(s) 175, 259
LmnI GCTCC 3 cut(s) 228, 249, 481
LweI GCATC 3 cut(s) 192, 565, 608
MaeI CTAG 3 cut(s) 135, 555, 636
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 4 cut(s) 107, 145, 303, 625
MalI GATC 2 cut(s) 177, 261
MboI GATC 2 cut(s) 175, 259
MboII GAAGA 6 cut(s) 37, 51, 54, 454, 506, 515
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 2 cut(s) 401, 518
MlyI GAGTC 2 cut(s) 125, 142
MnlI CCTC 8 cut(s) 36, 108, 184, 427, 490, 505, 600, 661
MspI CCGG 2 cut(s) 315, 369
MspR9I CCNGG 3 cut(s) 316, 370, 420
MvaI CCWGG 1 cut(s) 420
MvnI CGCG 1 cut(s) 563
MwoI GCNNNNNNNGC 1 cut(s) 17
NciI CCSGG 2 cut(s) 316, 370
NcoI CCATGG 1 cut(s) 356
NdeII GATC 2 cut(s) 175, 259
NlaIII CATG 2 cut(s) 360, 418
NlaIV GGNNCC 1 cut(s) 230
NmuCI GTSAC 2 cut(s) 107, 145
NsbI TGCGCA 1 cut(s) 654
NspI RCATGY 1 cut(s) 418
PkrI GCNGC 2 cut(s) 76, 233
PleI GAGTC 2 cut(s) 125, 142
PpsI GAGTC 2 cut(s) 125, 142
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 230
PspPI GGNCC 1 cut(s) 207
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 1 cut(s) 678
RsaNI GTAC 1 cut(s) 677
SatI GCNGC 2 cut(s) 75, 232
Sau3AI GATC 2 cut(s) 175, 259
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 2 cut(s) 125, 142
ScrFI CCNGG 3 cut(s) 316, 370, 420
SduI GDGCHC 1 cut(s) 486
SetI ASST 7 cut(s) 16, 119, 147, 202, 332, 345, 672
SfaNI GCATC 3 cut(s) 192, 565, 608
SfcI CTRYAG 1 cut(s) 126
SinI GGWCC 1 cut(s) 207
SpeI ACTAGT 1 cut(s) 554
Sse9I AATT 2 cut(s) 401, 518
SsiI CCGC 3 cut(s) 75, 232, 406
SspMI CTAG 3 cut(s) 135, 555, 636
StyD4I CCNGG 3 cut(s) 314, 368, 418
StyI CCWWGG 2 cut(s) 356, 429
TaiI ACGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 262
TasI AATT 2 cut(s) 401, 518
TatI WGTACW 1 cut(s) 676
TauI GCSGC 2 cut(s) 77, 234
TseFI GTSAC 2 cut(s) 107, 145
Tsp45I GTSAC 2 cut(s) 107, 145
TspDTI ATGAA 1 cut(s) 443
TspGWI ACGGA 2 cut(s) 474, 524
VpaK11BI GGWCC 1 cut(s) 207
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 418
XspI CTAG 3 cut(s) 135, 555, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.