FvH4_6g21590

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014172
Physical Location & Seq
Forward (+)
15152230 .. 15153478
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21590.t1

Sequence Viewer

Length: 513 bp
ATGTCTCCTTCACCTGGGACGACGTCGTCAGGATTTCCCAGTCGGAAGCCCTCCCCGACGACCCTTCCGATCTCAGAGGGTACACTGATAGGATCAAAGCCTACAACCGTGGAGTCCCTTGAAAGAGCGTTGATGCAAGCAACAGGGAACATAAACAGGCTGTTTCAGAACCTAAGCTTATCACCGTGGTTGGTATCCGGGCGTGTCAAAGAGCAAGACGACTGCTACAAGTTGCAGTTCGACATGCCGGGACTTACCAAGGAGGATGTGAAGATCAGTGTTCATCATGGGAATCTGACAATCAAAGGGGAGCACAAGGCAGAAGAGGGAGAAGAGACAGACGATGAGTTCTGGTCATCAAGGAGCTATGGTTATTATAACACTAGTTTGGCGTTGCCGGATGATGCTAAAGTTGATGAGATAAAGGCATCGTTGAAGGATGGAGTGCTGTCTGTTACCATTCCTAGAAGTGAGAAGCCTAAGGAAGATGTGAAGGAGGTGAATATAAACTGA

Protein Analysis

171

Amino Acids

18.71

Weight (kDa)

5.14

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 69 - 170 4.5e-28 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52560 AT1G52560
fragaria_vesca FvH4_6g21590 FvH4_6g29880
malus_domestica MD17G1209800.v1.1
prunus_persica Prupe.3G085200_v2.0.a1
pyrus_communis pycom17g21520
rosa_chinensis RchiOBHm_Chr2g0135771
rosa_laevigata RLG00000019484
rosa_multiflora Rmu_sc0007848.1_g000021
rosa_roxburghii Rroxscaffold_2G00108840
rosa_rugosa Rorug02G0326300
rosa_samantha Rh2AG378000 Rh2BG384300 Rh2CG363700 Rh2DG400800
rosa_wichuraiana Rw2G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
AasI GACNNNNNNGTC 1 cut(s) 25
AatII GACGTC 1 cut(s) 26
AclWI GGATC 1 cut(s) 100
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 1 cut(s) 14
AgsI TTSAA 2 cut(s) 122, 436
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 2 cut(s) 177, 366
AluI AGCT 2 cut(s) 177, 366
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 9, 329
AlwI GGATC 1 cut(s) 100
AsuC2I CCSGG 2 cut(s) 199, 249
AsuHPI GGTGA 3 cut(s) 3, 174, 511
AxyI CCTNAGG 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 315
BccI CCATC 1 cut(s) 434
BciT130I CCWGG 1 cut(s) 15
BciVI GTATCC 1 cut(s) 205
BcnI CCSGG 2 cut(s) 199, 249
BcoDI GTCTC 2 cut(s) 9, 329
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 2 cut(s) 384, 465
BfuI GTATCC 1 cut(s) 205
Bme1390I CCNGG 3 cut(s) 15, 199, 249
BmrFI CCNGG 3 cut(s) 15, 199, 249
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 3 cut(s) 123, 394, 437
BmuI ACTGGG 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 173
BpuMI CCSGG 2 cut(s) 199, 249
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 4 cut(s) 14, 108, 185, 258
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 39
Bse21I CCTNAGG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 4 cut(s) 14, 108, 185, 258
BseGI GGATG 3 cut(s) 271, 406, 445
BseLI CCNNNNNNNGG 1 cut(s) 14
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 3 cut(s) 198, 248, 398
BslFI GGGAC 3 cut(s) 31, 100, 264
BslI CCNNNNNNNGG 1 cut(s) 14
BsmAI GTCTC 2 cut(s) 9, 329
BsmFI GGGAC 3 cut(s) 31, 100, 264
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 69, 92, 273
BspCNI CTCAG 1 cut(s) 86
BspPI GGATC 1 cut(s) 100
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 4 cut(s) 14, 108, 185, 258
BssMI GATC 3 cut(s) 69, 92, 273
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 2 cut(s) 109, 186
Bst6I CTCTTC 2 cut(s) 318, 327
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 73, 173, 480
BstDSI CCRYGG 2 cut(s) 108, 185
BstF5I GGATG 3 cut(s) 271, 406, 445
BstKTI GATC 3 cut(s) 72, 95, 276
BstMAI GTCTC 2 cut(s) 9, 329
BstMBI GATC 3 cut(s) 69, 92, 273
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 1 cut(s) 247
BstSCI CCNGG 3 cut(s) 13, 197, 247
Bsu36I CCTNAGG 1 cut(s) 480
BsuI GTATCC 1 cut(s) 205
BtgI CCRYGG 2 cut(s) 108, 185
BtsCI GGATG 3 cut(s) 271, 406, 445
BtsIMutI CAGTG 2 cut(s) 83, 283
Cac8I GCNNGC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 81
CviAII CATG 2 cut(s) 244, 287
CviJI RGCY 6 cut(s) 49, 100, 160, 177, 366, 478
CviKI_1 RGCY 6 cut(s) 49, 100, 160, 177, 366, 478
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 3 cut(s) 73, 173, 480
DpnI GATC 3 cut(s) 71, 94, 275
DpnII GATC 3 cut(s) 69, 92, 273
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
Eam1104I CTCTTC 2 cut(s) 318, 327
EarI CTCTTC 2 cut(s) 318, 327
Eco130I CCWWGG 1 cut(s) 258
Eco81I CCTNAGG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 2 cut(s) 247, 290
FaiI YATR 6 cut(s) 152, 245, 288, 369, 378, 506
FaqI GGGAC 3 cut(s) 31, 100, 264
FatI CATG 2 cut(s) 243, 286
FokI GGATG 3 cut(s) 278, 413, 452
FspBI CTAG 2 cut(s) 384, 465
HapII CCGG 3 cut(s) 198, 248, 398
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 2 cut(s) 247, 290
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 2 cut(s) 113, 292
HpaII CCGG 3 cut(s) 198, 248, 398
HphI GGTGA 3 cut(s) 3, 174, 511
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 5 cut(s) 45, 69, 76, 168, 297
Hpy188III TCNNGA 1 cut(s) 30
Hpy8I GTNNAC 1 cut(s) 83
Hpy99I CGWCG 3 cut(s) 25, 28, 61
HpyAV CCTTC 4 cut(s) 18, 74, 430, 487
HpyCH4III ACNGT 2 cut(s) 109, 186
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 136, 235
HpyF3I CTNAG 3 cut(s) 73, 173, 480
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 2 cut(s) 247, 290
Kzo9I GATC 3 cut(s) 69, 92, 273
LmnI GCTCC 2 cut(s) 310, 363
LpnPI CCDG 9 cut(s) 15, 27, 52, 129, 142, 211, 261, 337, 411
LweI GCATC 3 cut(s) 123, 394, 437
MaeI CTAG 2 cut(s) 384, 465
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 454
MalI GATC 3 cut(s) 71, 94, 275
MboI GATC 3 cut(s) 69, 92, 273
MboII GAAGA 4 cut(s) 283, 335, 344, 497
MhlI GDGCHC 1 cut(s) 315
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 1 cut(s) 23
MnlI CCTC 5 cut(s) 61, 70, 256, 319, 490
MspI CCGG 3 cut(s) 198, 248, 398
MspR9I CCNGG 3 cut(s) 15, 199, 249
MvaI CCWGG 1 cut(s) 15
NciI CCSGG 2 cut(s) 199, 249
NdeII GATC 3 cut(s) 69, 92, 273
NlaIII CATG 2 cut(s) 247, 290
NspI RCATGY 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PfeI GAWTC 1 cut(s) 292
PflFI GACNNNGTC 2 cut(s) 22, 25
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
PsiI TTATAA 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PsyI GACNNNGTC 2 cut(s) 22, 25
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
Sau3AI GATC 3 cut(s) 69, 92, 273
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 3 cut(s) 15, 199, 249
SduI GDGCHC 1 cut(s) 315
SetI ASST 6 cut(s) 16, 26, 174, 179, 368, 501
SfaNI GCATC 3 cut(s) 123, 394, 437
SpeI ACTAGT 1 cut(s) 383
SspMI CTAG 2 cut(s) 384, 465
StyD4I CCNGG 3 cut(s) 13, 197, 247
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 2 cut(s) 109, 186
TaiI ACGT 1 cut(s) 26
TaqI TCGA 1 cut(s) 240
TfiI GAWTC 1 cut(s) 292
TscAI CASTG 2 cut(s) 90, 283
TspDTI ATGAA 1 cut(s) 272
TspRI CASTG 2 cut(s) 90, 283
Tth111I GACNNNGTC 2 cut(s) 22, 25
XceI RCATGY 1 cut(s) 247
XspI CTAG 2 cut(s) 384, 465
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.