FvH4_6g29880

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014172
Physical Location & Seq
Reverse (-)
23094955 .. 23096725
1771 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29880.t1

Sequence Viewer

Length: 690 bp
ATGGCTTTGGCACGTTTGGCTTTGAAGAGACACTTGCAGCAGAGGGTGCTTTCTCCATCTCCTCCTTTCTCTGCAGCTAATTCTGTGTTATTGGAAGGCCGTGGTGGTGGTGAGAGGAGGTTGCTTGGTACAGACGCTACTGATAAGGTGAGTATCCGTGAGAAGACAGCCGATGACAAAGACCTTAGCATCTCTGAAGCTAAAGGTAGAAGGGCCAACTTGTTCCCCAACTGGAGCCGCAGTAGGGGATTATGGGGCAGTAGCGATCAGGACTTTGTTCCTGCCCTTTCCGGCCTTGAAAGAGCGTTGATGCAAGCAACAGGGAACATAAACAGGCTGTTTGAGAACCTAAGCTTATCACCGTGGTCAGTATCCGGGCGTGTCAAAGAGCAAGACGACTGCTACAAGTTGCGATTCGACATGCCGGGACTTACCAAGGAGGATGTGAAGATCAGTGTTCATCATGGGAATATGACAATCAAGGGGGAGCACAAGGAAGAAGAGGGAGAAGAGACAGACGATGAGTTCTGGTCATCAAGGAGCTATGGTTATTATAACACTAGTTTGGCGTTGCCGGATGATGCTAAAGTCGATGAGATAAAGGCATCGTTGAAGGATGGGGTGCTATCTGTTACCATTCCTAGAAGTGAGAAGCCTAAGGAAGATGTGAAGGAGGTGAATATAAACTGA

Protein Analysis

230

Amino Acids

25.53

Weight (kDa)

5.9

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 128 - 229 2.6e-27 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52560 AT1G52560
fragaria_vesca FvH4_6g21590 FvH4_6g29880
malus_domestica MD17G1209800.v1.1
prunus_persica Prupe.3G085200_v2.0.a1
pyrus_communis pycom17g21520
rosa_chinensis RchiOBHm_Chr2g0135771
rosa_laevigata RLG00000019484
rosa_multiflora Rmu_sc0007848.1_g000021
rosa_roxburghii Rroxscaffold_2G00108840
rosa_rugosa Rorug02G0326300
rosa_samantha Rh2AG378000 Rh2BG384300 Rh2CG363700 Rh2DG400800
rosa_wichuraiana Rw2G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AciI CCGC 1 cut(s) 238
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 1 cut(s) 130
AfiI CCNNNNNNNGG 1 cut(s) 244
AgsI TTSAA 3 cut(s) 25, 299, 613
AhlI ACTAGT 1 cut(s) 560
AluBI AGCT 4 cut(s) 77, 200, 354, 543
AluI AGCT 4 cut(s) 77, 200, 354, 543
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 2 cut(s) 22, 506
AoxI GGCC 3 cut(s) 97, 213, 292
ApeKI GCWGC 2 cut(s) 37, 74
AspS9I GGNCC 1 cut(s) 213
AsuC2I CCSGG 2 cut(s) 376, 426
AsuHPI GGTGA 4 cut(s) 122, 160, 351, 688
AxyI CCTNAGG 1 cut(s) 657
BbsI GAAGAC 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 49, 86
BccI CCATC 2 cut(s) 64, 611
BceAI ACGGC 1 cut(s) 84
BciVI GTATCC 2 cut(s) 164, 382
BcnI CCSGG 2 cut(s) 376, 426
BcoDI GTCTC 2 cut(s) 22, 506
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 2 cut(s) 561, 642
BfmI CTRYAG 1 cut(s) 72
BfuI GTATCC 2 cut(s) 164, 382
BisI GCNGC 3 cut(s) 38, 75, 238
BlsI GCNGC 3 cut(s) 39, 76, 239
Bme1390I CCNGG 2 cut(s) 376, 426
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 1 cut(s) 236
BmrFI CCNGG 2 cut(s) 376, 426
BmsI GCATC 4 cut(s) 198, 300, 571, 614
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 1 cut(s) 253
Bpu10I CCTNAGC 2 cut(s) 185, 350
BpuMI CCSGG 2 cut(s) 376, 426
BsaJI CCNNGG 3 cut(s) 100, 362, 435
BsaXI ACNNNNNCTCC 4 cut(s) 226, 256, 532, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse1I ACTGG 1 cut(s) 236
Bse21I CCTNAGG 1 cut(s) 657
BseDI CCNNGG 3 cut(s) 100, 362, 435
BseGI GGATG 3 cut(s) 448, 583, 622
BseLI CCNNNNNNNGG 1 cut(s) 244
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 2 cut(s) 51, 130
BseXI GCAGC 2 cut(s) 49, 86
BshFI GGCC 3 cut(s) 99, 215, 294
BsiHKAI GWGCWC 1 cut(s) 492
BsiSI CCGG 4 cut(s) 291, 375, 425, 575
BslFI GGGAC 1 cut(s) 441
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 2 cut(s) 22, 506
BsmFI GGGAC 1 cut(s) 441
BsnI GGCC 3 cut(s) 99, 215, 294
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 2 cut(s) 265, 450
BspACI CCGC 1 cut(s) 238
BspANI GGCC 3 cut(s) 99, 215, 294
BspLI GGNNCC 1 cut(s) 236
BspMAI CTGCAG 1 cut(s) 76
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 3 cut(s) 100, 362, 435
BssMI GATC 2 cut(s) 265, 450
BssT1I CCWWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 3 cut(s) 20, 495, 504
BstAPI GCANNNNNTGC 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 315
BstDEI CTNAG 3 cut(s) 185, 350, 657
BstDSI CCRYGG 2 cut(s) 100, 362
BstF5I GGATG 3 cut(s) 448, 583, 622
BstKTI GATC 2 cut(s) 268, 453
BstMAI GTCTC 2 cut(s) 22, 506
BstMBI GATC 2 cut(s) 265, 450
BstMWI GCNNNNNNNGC 2 cut(s) 17, 46
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 374, 424
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 2 cut(s) 49, 86
BstV2I GAAGAC 1 cut(s) 170
Bsu36I CCTNAGG 1 cut(s) 657
BsuI GTATCC 2 cut(s) 164, 382
BsuRI GGCC 3 cut(s) 99, 215, 294
BtgI CCRYGG 2 cut(s) 100, 362
BtsCI GGATG 3 cut(s) 448, 583, 622
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 315
Cfr13I GGNCC 1 cut(s) 213
CseI GACGC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 129
CviAII CATG 2 cut(s) 421, 464
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 3 cut(s) 185, 350, 657
DpnI GATC 2 cut(s) 267, 452
DpnII GATC 2 cut(s) 265, 450
Eam1104I CTCTTC 3 cut(s) 20, 495, 504
EarI CTCTTC 3 cut(s) 20, 495, 504
Eco130I CCWWGG 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 216
Eco81I CCTNAGG 1 cut(s) 657
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 2 cut(s) 424, 467
FaiI YATR 8 cut(s) 253, 329, 422, 465, 473, 546, 555, 683
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FaqI GGGAC 1 cut(s) 441
FatI CATG 2 cut(s) 420, 463
Fnu4HI GCNGC 3 cut(s) 38, 75, 238
FokI GGATG 3 cut(s) 455, 590, 629
Fsp4HI GCNGC 3 cut(s) 38, 75, 238
FspBI CTAG 2 cut(s) 561, 642
GluI GCNGC 3 cut(s) 38, 75, 238
GsuI CTGGAG 1 cut(s) 253
HaeIII GGCC 3 cut(s) 99, 215, 294
HapII CCGG 4 cut(s) 291, 375, 425, 575
HgaI GACGC 1 cut(s) 143
Hin1II CATG 2 cut(s) 424, 467
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 1 cut(s) 414
HpaII CCGG 4 cut(s) 291, 375, 425, 575
HphI GGTGA 4 cut(s) 122, 160, 351, 688
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 269
HpyAV CCTTC 4 cut(s) 89, 204, 607, 664
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 3 cut(s) 37, 74, 313
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 46
HpyF3I CTNAG 3 cut(s) 185, 350, 657
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 2 cut(s) 424, 467
Kzo9I GATC 2 cut(s) 265, 450
LmnI GCTCC 3 cut(s) 234, 487, 540
Lsp1109I GCAGC 2 cut(s) 49, 86
LweI GCATC 4 cut(s) 198, 300, 571, 614
MaeI CTAG 2 cut(s) 561, 642
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 1 cut(s) 631
MalI GATC 2 cut(s) 267, 452
MboI GATC 2 cut(s) 265, 450
MboII GAAGA 7 cut(s) 37, 175, 460, 509, 512, 521, 674
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 1 cut(s) 79
MnlI CCTC 7 cut(s) 36, 72, 108, 111, 433, 496, 667
MspI CCGG 4 cut(s) 291, 375, 425, 575
MspR9I CCNGG 2 cut(s) 376, 426
MwoI GCNNNNNNNGC 2 cut(s) 17, 46
NciI CCSGG 2 cut(s) 376, 426
NdeII GATC 2 cut(s) 265, 450
NlaIII CATG 2 cut(s) 424, 467
NlaIV GGNNCC 1 cut(s) 236
NspI RCATGY 1 cut(s) 424
PfeI GAWTC 1 cut(s) 414
PkrI GCNGC 3 cut(s) 39, 76, 239
PsiI TTATAA 1 cut(s) 555
PspN4I GGNNCC 1 cut(s) 236
PspPI GGNCC 1 cut(s) 213
PstI CTGCAG 1 cut(s) 76
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
SatI GCNGC 3 cut(s) 38, 75, 238
Sau3AI GATC 2 cut(s) 265, 450
Sau96I GGNCC 1 cut(s) 213
ScrFI CCNGG 2 cut(s) 376, 426
SduI GDGCHC 1 cut(s) 492
SfaNI GCATC 4 cut(s) 198, 300, 571, 614
SfcI CTRYAG 1 cut(s) 72
SpeI ACTAGT 1 cut(s) 560
Sse9I AATT 1 cut(s) 79
SsiI CCGC 1 cut(s) 238
SspMI CTAG 2 cut(s) 561, 642
StyD4I CCNGG 2 cut(s) 374, 424
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 363
TaiI ACGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 417, 591
TasI AATT 1 cut(s) 79
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 1 cut(s) 414
TscAI CASTG 1 cut(s) 460
TseI GCWGC 2 cut(s) 37, 74
TspDTI ATGAA 1 cut(s) 449
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 1 cut(s) 460
XceI RCATGY 1 cut(s) 424
XspI CTAG 2 cut(s) 561, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.