Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0020216

Physical Location & Seq

Genomic Coordinates Reverse (-)

22229780 .. 22230748

Gene Structure 969 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G60300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 969 bp

ATGGAGGACGACGACGCAGCTTATGATCTAATCAAACACGAACTGTTATACTCAGAAGACGAAGTAATAATCTCACGTTATCTGAAGGGTATGGTCGTTAACGGAGATTCTTGGCCAGATCACTTCATCGAAGACGCAAACGTGTTCACCAAGAATCCAGATAAGGTGTTCAATTCTGAGAGACCTAGATTCGTGATCGTTAAACCACGAACAGAGGCTTGTGGTAAAACCGATGGATGTGATTCGGGTTGCTGGAGGATCATTGGTCGTGATAAACTGATAAAGTCGGAGGAGACTGGGAAGATTCTAGGGTTCAAGAAGATACTCAAGTTTTGCCTAAAGAGGAAACCTATAGACTACAAGAGAAGTTGGGTAATGGAAGAGTATAGGCTTACCAATAACTTGAACTGGAAGCAAGATCATGTGATTTGCAAAATTCGGTTTATGTTTGAAGCTGAAATTAGTTTCTTGCTAAGCAAGCATTTCTACACTACATCAGAATCGGTTCTTGAAAATGAGCTGTTGCCATCTTATGGATATTATTTATCCAATACACAAGAGGAGGATGAATTTTATCTGGACGCGATAATGACTTCGGAAGGAAACGAGTGGCCTAGCTACGTTACCAACAACGTGTACTGTCTGCATCCATTGGAGCTTGTGGATCTTCAAGATCGGATGTTTAATGATTACGGAACCTGCATCTTCGCTAACAAGACTTGTGGTGAAACTGATAAATGCGATGGTGGTTACTGGAAGATCCTGCACGGTGATAAGCTGATCAAGTCAAATTTCGGAAAGGTCATTGGTTTCAAGAAGGTATTTGAGTTCTATGAAACGGTGAGACAAATATATCTTTGTGATGGAGAAGAAGTGACGGTAACTTGGACTATACAAGAGTATAGGCTTAGCAAAAACGTGAAGCAGAATAAAGTGTTGTGCGTTATCAAGTTGACTTATGATAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009825 GO:0009835 GO:0009889 GO:0009908 GO:0009987 GO:0010150 GO:0010154 GO:0010468 GO:0010556 GO:0016049 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071695 GO:0080090 GO:0090567 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

322

Amino Acids

37.85

Weight (kDa)

5.46

Isoelectric Point (pI)

27.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	19 - 131	8.3e-14	No apical meristem (NAM) protein
NAM	PF02365	189 - 303	1.5e-08	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0020216)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G60240 AT1G60280 AT1G60300 AT1G60340 AT1G60350 AT1G60380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	707
AccB7I	CCANNNNNTGG	1 cut(s)	533
AccII	CGCG	1 cut(s)	584
AclWI	GGATC	3 cut(s)	266, 672, 754
AcoI	YGGCCR	1 cut(s)	113
AcsI	RAATTY	3 cut(s)	435, 569, 790
AcuI	CTGAAG	1 cut(s)	104
AfaI	GTAC	1 cut(s)	638
AfiI	CCNNNNNNNGG	1 cut(s)	533
AflIII	ACRYGT	2 cut(s)	141, 633
AgsI	TTSAA	7 cut(s)	172, 316, 406, 452, 512, 671, 814
AluBI	AGCT	6 cut(s)	20, 455, 520, 618, 658, 778
AluI	AGCT	6 cut(s)	20, 455, 520, 618, 658, 778
Alw26I	GTCTC	3 cut(s)	175, 287, 838
AlwI	GGATC	3 cut(s)	266, 672, 754
AoxI	GGCC	2 cut(s)	113, 611
ApeKI	GCWGC	1 cut(s)	17
ApoI	RAATTY	3 cut(s)	435, 569, 790
Asp700I	GAANNNNTTC	1 cut(s)	504
AsuHPI	GGTGA	4 cut(s)	139, 737, 782, 853
BaeI	ACNNNNGTAYC	2 cut(s)	314, 347
BalI	TGGCCA	1 cut(s)	115
BbsI	GAAGAC	2 cut(s)	63, 138
BbvI	GCAGC	1 cut(s)	29
BccI	CCATC	4 cut(s)	227, 535, 737, 857
BclI	TGATCA	1 cut(s)	780
BcoDI	GTCTC	3 cut(s)	175, 287, 838
BfaI	CTAG	3 cut(s)	186, 308, 615
BfmI	CTRYAG	1 cut(s)	351
BfuAI	ACCTGC	1 cut(s)	707
BisI	GCNGC	1 cut(s)	18
BlpI	GCTNAGC	2 cut(s)	473, 908
BlsI	GCNGC	1 cut(s)	19
BmiI	GGNNCC	1 cut(s)	697
BmrI	ACTGGG	1 cut(s)	306
BmsI	GCATC	2 cut(s)	655, 711
BmuI	ACTGGG	1 cut(s)	306
BpiI	GAAGAC	2 cut(s)	63, 138
BpmI	CTGGAG	1 cut(s)	274
Bpu1102I	GCTNAGC	2 cut(s)	473, 908
BpuEI	CTTGAG	1 cut(s)	311
BsaI	GGTCTC	1 cut(s)	175
BsaXI	ACNNNNNCTCC	2 cut(s)	858, 888
Bsc4I	CCNNNNNNNGG	1 cut(s)	533
Bse1I	ACTGG	3 cut(s)	301, 413, 758
BseGI	GGATG	4 cut(s)	242, 571, 646, 684
BseLI	CCNNNNNNNGG	1 cut(s)	533
BseMII	CTCAG	2 cut(s)	66, 168
BseNI	ACTGG	3 cut(s)	301, 413, 758
BseRI	GAGGAG	2 cut(s)	305, 575
BseXI	GCAGC	1 cut(s)	29
BsgI	GTGCAG	1 cut(s)	749
Bsh1236I	CGCG	1 cut(s)	584
BshFI	GGCC	2 cut(s)	115, 613
BslI	CCNNNNNNNGG	1 cut(s)	533
BsmAI	GTCTC	3 cut(s)	175, 287, 838
BsnI	GGCC	2 cut(s)	115, 613
Bso31I	GGTCTC	1 cut(s)	175
Bsp143I	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
Bsp1720I	GCTNAGC	2 cut(s)	473, 908
BspANI	GGCC	2 cut(s)	115, 613
BspCNI	CTCAG	2 cut(s)	65, 169
BspFNI	CGCG	1 cut(s)	584
BspLI	GGNNCC	1 cut(s)	697
BspMI	ACCTGC	1 cut(s)	707
BspPI	GGATC	3 cut(s)	266, 672, 754
BspTNI	GGTCTC	1 cut(s)	175
BsrI	ACTGG	3 cut(s)	301, 413, 758
BssMI	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
Bst4CI	ACNGT	5 cut(s)	45, 641, 770, 841, 880
Bst6I	CTCTTC	1 cut(s)	375
BstC8I	GCNNGC	1 cut(s)	479
BstDEI	CTNAG	4 cut(s)	52, 177, 473, 908
BstF5I	GGATG	4 cut(s)	242, 571, 646, 684
BstFNI	CGCG	1 cut(s)	584
BstKTI	GATC	9 cut(s)	28, 121, 198, 261, 421, 667, 676, 762, 783
BstMAI	GTCTC	3 cut(s)	175, 287, 838
BstMBI	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
BstMWI	GCNNNNNNNGC	1 cut(s)	478
BstSFI	CTRYAG	1 cut(s)	351
BstUI	CGCG	1 cut(s)	584
BstV1I	GCAGC	1 cut(s)	29
BstV2I	GAAGAC	2 cut(s)	63, 138
BstX2I	RGATCY	2 cut(s)	664, 759
BstYI	RGATCY	2 cut(s)	664, 759
BsuRI	GGCC	2 cut(s)	115, 613
BtgZI	GCGATG	1 cut(s)	756
BtsCI	GGATG	4 cut(s)	242, 571, 646, 684
BveI	ACCTGC	1 cut(s)	707
Cac8I	GCNNGC	1 cut(s)	479
CseI	GACGC	3 cut(s)	23, 143, 590
Csp6I	GTAC	1 cut(s)	637
CspCI	CAANNNNNGTGG	2 cut(s)	703, 738
CviAII	CATG	1 cut(s)	422
CviQI	GTAC	1 cut(s)	637
DdeI	CTNAG	4 cut(s)	52, 177, 473, 908
DpnI	GATC	9 cut(s)	27, 120, 197, 260, 420, 666, 675, 761, 782
DpnII	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
EaeI	YGGCCR	1 cut(s)	113
Eam1104I	CTCTTC	1 cut(s)	375
EarI	CTCTTC	1 cut(s)	375
Eco31I	GGTCTC	1 cut(s)	175
Eco57I	CTGAAG	1 cut(s)	104
FaeI	CATG	1 cut(s)	425
FatI	CATG	1 cut(s)	421
FbaI	TGATCA	1 cut(s)	780
Fnu4HI	GCNGC	1 cut(s)	18
FokI	GGATG	4 cut(s)	249, 578, 633, 691
Fsp4HI	GCNGC	1 cut(s)	18
FspBI	CTAG	3 cut(s)	186, 308, 615
GluI	GCNGC	1 cut(s)	18
GsuI	CTGGAG	1 cut(s)	274
HaeIII	GGCC	2 cut(s)	115, 613
HgaI	GACGC	3 cut(s)	23, 143, 590
Hin1II	CATG	1 cut(s)	425
HincII	GTYRAC	2 cut(s)	100, 954
HindII	GTYRAC	2 cut(s)	100, 954
HinfI	GANTC	6 cut(s)	107, 154, 189, 242, 304, 500
HpaI	GTTAAC	1 cut(s)	100
HphI	GGTGA	4 cut(s)	139, 737, 782, 853
Hpy166II	GTNNAC	4 cut(s)	100, 147, 637, 954
Hpy188I	TCNGA	8 cut(s)	55, 84, 178, 289, 499, 598, 678, 797
Hpy188III	TCNNGA	8 cut(s)	158, 193, 269, 316, 509, 578, 671, 814
Hpy8I	GTNNAC	4 cut(s)	100, 147, 637, 954
Hpy99I	CGWCG	2 cut(s)	14, 17
HpyAV	CCTTC	3 cut(s)	79, 593, 811
HpyCH4III	ACNGT	5 cut(s)	45, 641, 770, 841, 880
HpyCH4IV	ACGT	5 cut(s)	76, 141, 621, 633, 918
HpyCH4V	TGCA	4 cut(s)	432, 646, 702, 766
HpyF10VI	GCNNNNNNNGC	1 cut(s)	478
HpyF3I	CTNAG	4 cut(s)	52, 177, 473, 908
HpySE526I	ACGT	5 cut(s)	76, 141, 621, 633, 918
Hsp92II	CATG	1 cut(s)	425
Ksp22I	TGATCA	1 cut(s)	780
KspAI	GTTAAC	1 cut(s)	100
Kzo9I	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
LmnI	GCTCC	1 cut(s)	655
LpnPI	CCDG	9 cut(s)	129, 171, 238, 282, 394, 563, 712, 739, 776
Lsp1109I	GCAGC	1 cut(s)	29
LweI	GCATC	2 cut(s)	655, 711
MaeI	CTAG	3 cut(s)	186, 308, 615
MaeII	ACGT	5 cut(s)	76, 141, 621, 633, 918
MaeIII	GTNAC	4 cut(s)	622, 749, 874, 880
MalI	GATC	9 cut(s)	27, 120, 197, 260, 420, 666, 675, 761, 782
MboI	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
MboII	GAAGA	9 cut(s)	68, 143, 313, 331, 392, 659, 697, 769, 881
MflI	RGATCY	2 cut(s)	664, 759
MlsI	TGGCCA	1 cut(s)	115
MluCI	AATT	5 cut(s)	172, 435, 459, 569, 790
MluNI	TGGCCA	1 cut(s)	115
MmeI	TCCRAC	1 cut(s)	267
MnlI	CCTC	6 cut(s)	208, 249, 283, 336, 553, 556
Mox20I	TGGCCA	1 cut(s)	115
MroXI	GAANNNNTTC	1 cut(s)	504
MscI	TGGCCA	1 cut(s)	115
MseI	TTAA	3 cut(s)	99, 201, 684
Msp20I	TGGCCA	1 cut(s)	115
MvnI	CGCG	1 cut(s)	584
MwoI	GCNNNNNNNGC	1 cut(s)	478
NdeII	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
NlaIII	CATG	1 cut(s)	425
NlaIV	GGNNCC	1 cut(s)	697
NmuCI	GTSAC	1 cut(s)	874
PdmI	GAANNNNTTC	1 cut(s)	504
PfeI	GAWTC	6 cut(s)	107, 154, 189, 242, 304, 500
PflMI	CCANNNNNTGG	1 cut(s)	533
PkrI	GCNGC	1 cut(s)	19
PspN4I	GGNNCC	1 cut(s)	697
PsuI	RGATCY	2 cut(s)	664, 759
RsaI	GTAC	1 cut(s)	638
RsaNI	GTAC	1 cut(s)	637
SaqAI	TTAA	3 cut(s)	99, 201, 684
SatI	GCNGC	1 cut(s)	18
Sau3AI	GATC	9 cut(s)	25, 118, 195, 258, 418, 664, 673, 759, 780
SfaNI	GCATC	2 cut(s)	655, 711
SfcI	CTRYAG	1 cut(s)	351
SmlI	CTYRAG	1 cut(s)	326
SmoI	CTYRAG	1 cut(s)	326
Sse9I	AATT	5 cut(s)	172, 435, 459, 569, 790
SspMI	CTAG	3 cut(s)	186, 308, 615
TaaI	ACNGT	5 cut(s)	45, 641, 770, 841, 880
TaiI	ACGT	5 cut(s)	79, 144, 624, 636, 921
TaqI	TCGA	1 cut(s)	129
TasI	AATT	5 cut(s)	172, 435, 459, 569, 790
TatI	WGTACW	1 cut(s)	636
TfiI	GAWTC	6 cut(s)	107, 154, 189, 242, 304, 500
Tru1I	TTAA	3 cut(s)	99, 201, 684
Tru9I	TTAA	3 cut(s)	99, 201, 684
TseFI	GTSAC	1 cut(s)	874
TseI	GCWGC	1 cut(s)	17
Tsp45I	GTSAC	1 cut(s)	874
TspDTI	ATGAA	3 cut(s)	115, 582, 849
TspGWI	ACGGA	2 cut(s)	117, 708
Van91I	CCANNNNNTGG	1 cut(s)	533
XapI	RAATTY	3 cut(s)	435, 569, 790
XmnI	GAANNNNTTC	1 cut(s)	504
XspI	CTAG	3 cut(s)	186, 308, 615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.