AT1G65420

Protein of unknown function (DUF565)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011386
Physical Location & Seq
Reverse (-)
24297480 .. 24298608
1129 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65420.1

Sequence Viewer

Length: 594 bp
ATGGCTTGTCAAATTCAAGCTTCACGCGTTTTCCCAGTTCTAGAAATTGAAAAAGGTCTCTCCTTTATGATAAATGTCTTCCATAGGAGAATAAAAGCTTCGTCCATTACTCTCACTTCATTTCCTTATCCCATGAAAAGTTTTCAGATAAGAAGACCAAACCGGAAAATAGCGTTTGCGTTAGACACTGGTTCTTCTATTCCTGGAGATAGTGGTGAAGGGCAAGAGATGAATGGAGACAGAACCGGTCTCGGTAGCACTCGGTTAGGTAGAATTGCGATTGCAGGAGGCAAACAGTTACTTGGGAAGATAAACTCTGCAAGAAAAAACTTCCCAATGAAGATATTTCTGCTGCTTCTTGGTTTCTACACTGCCAATGCACTAGCTACTATTCTTGGACAGACTGGTGATTGGGATGTGCTTGTTGCCGGAATCGTCGTTGCTGCGATCGAAGGAATCGGTATGCTTATGTATAAGAAACCGTCTTCTTCAATGTTTTCAGGGAAGTTGCAGTCTTTTGTTGTGTTTATGAATTTTTGGAAAGCTGGTGTTTGCTTAGGTCTCTTTGTTGATGCTTTCAAGTTGGGTAGTTAA

Protein Analysis

197

Amino Acids

21.4

Weight (kDa)

10.11

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 134 - 197 4.8e-22 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011386)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65420
fragaria_vesca FvH4_6g00150 FvH4_6g00150 FvH4_6g00150
malus_domestica MD04G1247900.v1.1 MD12G1262600.v1.1
prunus_persica Prupe.6G364400_v2.0.a1 Prupe.6G364400_v2.0.a1 Prupe.6G364400_v2.0.a1
pyrus_communis pycom04g21860
rosa_chinensis RchiOBHm_Chr3g0447501
rosa_laevigata RLG00000025992
rosa_multiflora Rmu_sc0006572.1_g000003
rosa_roxburghii Rroxscaffold_6G00425720
rosa_rugosa Rorug02G0602400
rosa_samantha Rh3AG002600 Rh3BG002100 Rh3CG002400 Rh3DG002500
rosa_wichuraiana Rw3G000150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 27
AcsI RAATTY 2 cut(s) 12, 532
AflIII ACRYGT 1 cut(s) 25
AgeI ACCGGT 1 cut(s) 245
AgsI TTSAA 4 cut(s) 17, 50, 492, 580
AjnI CCWGG 1 cut(s) 202
AluBI AGCT 4 cut(s) 20, 98, 386, 545
AluI AGCT 4 cut(s) 20, 98, 386, 545
Alw26I GTCTC 4 cut(s) 62, 231, 254, 566
ApeKI GCWGC 2 cut(s) 352, 443
ApoI RAATTY 2 cut(s) 12, 532
AsiGI ACCGGT 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 227, 419
BbsI GAAGAC 3 cut(s) 70, 160, 477
BbvI GCAGC 2 cut(s) 339, 430
BciT130I CCWGG 1 cut(s) 204
BcoDI GTCTC 4 cut(s) 62, 231, 254, 566
BfaI CTAG 2 cut(s) 41, 383
BisI GCNGC 2 cut(s) 353, 444
BlsI GCNGC 2 cut(s) 354, 445
Bme1390I CCNGG 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 204
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 1 cut(s) 562
BmuI ACTGGG 1 cut(s) 29
BpiI GAAGAC 3 cut(s) 70, 160, 477
BpmI CTGGAG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 556
BsaI GGTCTC 3 cut(s) 62, 254, 566
BsaWI WCCGGW 2 cut(s) 162, 245
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 3 cut(s) 35, 193, 409
BseBI CCWGG 1 cut(s) 204
BseGI GGATG 1 cut(s) 421
BseNI ACTGG 3 cut(s) 35, 193, 409
BseXI GCAGC 2 cut(s) 339, 430
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 450
BshTI ACCGGT 1 cut(s) 245
BsiEI CGRYCG 1 cut(s) 450
BsiSI CCGG 3 cut(s) 163, 246, 429
BsmAI GTCTC 4 cut(s) 62, 231, 254, 566
Bso31I GGTCTC 3 cut(s) 62, 254, 566
Bsp143I GATC 1 cut(s) 447
BspFNI CGCG 1 cut(s) 27
BspTNI GGTCTC 3 cut(s) 62, 254, 566
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 3 cut(s) 35, 193, 409
BssAI RCCGGY 1 cut(s) 245
BssMI GATC 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 2 cut(s) 297, 483
BstDEI CTNAG 1 cut(s) 556
BstF5I GGATG 1 cut(s) 421
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 1 cut(s) 450
BstMAI GTCTC 4 cut(s) 62, 231, 254, 566
BstMBI GATC 1 cut(s) 447
BstMCI CGRYCG 1 cut(s) 450
BstNI CCWGG 1 cut(s) 204
BstSCI CCNGG 1 cut(s) 202
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 2 cut(s) 339, 430
BstV2I GAAGAC 3 cut(s) 70, 160, 477
BtsCI GGATG 1 cut(s) 421
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 186, 369
Cfr10I RCCGGY 1 cut(s) 245
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 1 cut(s) 133
CviJI RGCY 5 cut(s) 5, 20, 98, 386, 545
CviKI_1 RGCY 5 cut(s) 5, 20, 98, 386, 545
DdeI CTNAG 1 cut(s) 556
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
Eco31I GGTCTC 3 cut(s) 62, 254, 566
EcoRII CCWGG 1 cut(s) 202
FaeI CATG 1 cut(s) 136
FaiI YATR 7 cut(s) 68, 84, 134, 464, 470, 474, 530
FatI CATG 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 353, 444
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 2 cut(s) 353, 444
FspBI CTAG 2 cut(s) 41, 383
GluI GCNGC 2 cut(s) 353, 444
GsuI CTGGAG 1 cut(s) 225
HapII CCGG 3 cut(s) 163, 246, 429
Hin1II CATG 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 18, 96
HinfI GANTC 2 cut(s) 432, 456
HpaII CCGG 3 cut(s) 163, 246, 429
HphI GGTGA 2 cut(s) 227, 419
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 1 cut(s) 41
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 2 cut(s) 212, 446
HpyCH4III ACNGT 2 cut(s) 297, 483
HpyCH4V TGCA 4 cut(s) 284, 320, 380, 511
HpyF3I CTNAG 1 cut(s) 556
Hsp92II CATG 1 cut(s) 136
Kzo9I GATC 1 cut(s) 447
Lsp1109I GCAGC 2 cut(s) 339, 430
LweI GCATC 1 cut(s) 562
MaeI CTAG 2 cut(s) 41, 383
MaeIII GTNAC 1 cut(s) 297
MalI GATC 1 cut(s) 449
MboI GATC 1 cut(s) 447
MboII GAAGA 7 cut(s) 70, 165, 186, 319, 352, 477, 480
MluCI AATT 4 cut(s) 12, 45, 273, 532
MluI ACGCGT 1 cut(s) 25
MnlI CCTC 1 cut(s) 281
MseI TTAA 1 cut(s) 592
MspI CCGG 3 cut(s) 163, 246, 429
MspR9I CCNGG 1 cut(s) 204
MvaI CCWGG 1 cut(s) 204
MvnI CGCG 1 cut(s) 27
NdeII GATC 1 cut(s) 447
NlaIII CATG 1 cut(s) 136
PfeI GAWTC 2 cut(s) 432, 456
PfoI TCCNGGA 1 cut(s) 202
PinAI ACCGGT 1 cut(s) 245
PkrI GCNGC 2 cut(s) 354, 445
Ple19I CGATCG 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PvuI CGATCG 1 cut(s) 450
SaqAI TTAA 1 cut(s) 592
SatI GCNGC 2 cut(s) 353, 444
Sau3AI GATC 1 cut(s) 447
ScrFI CCNGG 1 cut(s) 204
SetI ASST 7 cut(s) 22, 58, 100, 271, 388, 547, 562
SfaNI GCATC 1 cut(s) 562
Sse9I AATT 4 cut(s) 12, 45, 273, 532
SspMI CTAG 2 cut(s) 41, 383
StyD4I CCNGG 1 cut(s) 202
TaaI ACNGT 2 cut(s) 297, 483
TaqI TCGA 1 cut(s) 450
TasI AATT 4 cut(s) 12, 45, 273, 532
TfiI GAWTC 2 cut(s) 432, 456
Tru1I TTAA 1 cut(s) 592
Tru9I TTAA 1 cut(s) 592
TscAI CASTG 2 cut(s) 193, 376
TseI GCWGC 2 cut(s) 352, 443
TspDTI ATGAA 5 cut(s) 108, 149, 245, 353, 545
TspRI CASTG 2 cut(s) 193, 376
XapI RAATTY 2 cut(s) 12, 532
XbaI TCTAGA 1 cut(s) 40
XspI CTAG 2 cut(s) 41, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.