Rorug02G0602400

ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011386
Physical Location & Seq
Reverse (-)
72135140 .. 72136255
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0602400.1

Sequence Viewer

Length: 1116 bp
ATGATCTTCTTAAGTGAGACTCGATGTACGGAGGAGGAGATGGATGGGGTGAGAAGACAGATAGGGTGGAAGAATTGTTTTGTGGTGGAATGTAAATTTGTGCCTAAAAAGAACAGTAAAGGAGTTAGTAGGTCTGGTGGATTGGCTCTACTTTGGGAAGAGGATGTGGTGGTTGATATACAATCATTCTCTGACAGTCATATTGATGTGGTAGTGGGGGAACCCTCTGATCCAAAACGGTGGAGATTTACCGGTTTTTATGGGCAACCAAGACCGGAGAACCGTCATCTATCATGGGCTTTGCTTAAAACCCTGAGTTTGCATGGCAATCTGCCATGGGTGGTCGGCGGCGATCTGAATGAAATTACTTCGACTGATGATAAGGAAGGTGGAGTAGTGAGGAATATACGACAGATGATGGGCCTGAGGGATGCACTAGACTTTGGCCTACTCTCAGACATCAAATTCTTGGGGCCGAGGTATACCTGGCAAGGCATTAGAGGGGGTCATCTCATTAAAATTCGACTCGACAGGTTTGTCGCCTCGCAGAGCTGGAAAAATTTATATTCGGCTTCTAGGGTTTTACATTTAAACCCTAGCACGTCAGATCATCTTCTAATCCTCCTGGAGGTCCGTGAGCACATGCAAACAAAGAGGAGGCGAGGGAGAAGATTCCGTTTTGAGGAATTATGGCTTAGGGAAGAGGATTGCAAGCAGATTGTTGAACAAAGCTGGGAGAGTGGTTCTGGGTCTGATGCGTTTTCAAAAATTTGTGATAAGATAAGGCGGACGCGGAGTGCCTTGGAATCTTGGAGTTTTGACAAGTTTAGGAGTCTTACCCGTACTATTGAAGATACTAGGGCTAGGTTAGCAGTTTTTTATGATACAAGGACGATACCTCCGCCCCCGGTATCGCTCAGGGTGGAGTTGGAAACCAAGCTACACATTCTTCTACAGCAAGAGATGTTATTTTGGAAACAGAGAGCGAAGATTTTCTGGCTGAAAGAGGGAGATGCTAACTGTTTGGCTCCAAAACCAGTTGGCTTGCAAACTGTCTCTTTTGAGCGAGTGATTGCGCAGGCGTGCCAGCACCGCGGGGTGCAGTCGTTGGGGTAG

Protein Analysis

371

Amino Acids

42.89

Weight (kDa)

9.31

Isoelectric Point (pI)

60.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 2 - 204 1e-10 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011386)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65420
fragaria_vesca FvH4_6g00150 FvH4_6g00150 FvH4_6g00150
malus_domestica MD04G1247900.v1.1 MD12G1262600.v1.1
prunus_persica Prupe.6G364400_v2.0.a1 Prupe.6G364400_v2.0.a1 Prupe.6G364400_v2.0.a1
pyrus_communis pycom04g21860
rosa_chinensis RchiOBHm_Chr3g0447501
rosa_laevigata RLG00000025992
rosa_multiflora Rmu_sc0006572.1_g000003
rosa_roxburghii Rroxscaffold_6G00425720
rosa_rugosa Rorug02G0602400
rosa_samantha Rh3AG002600 Rh3BG002100 Rh3CG002400 Rh3DG002500
rosa_wichuraiana Rw3G000150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 536
Acc16I TGCGCA 1 cut(s) 1077
AccI GTMKAC 1 cut(s) 482
AccII CGCG 2 cut(s) 793, 1095
AciI CCGC 6 cut(s) 348, 787, 793, 902, 1093, 1095
AclWI GGATC 1 cut(s) 224
AcsI RAATTY 5 cut(s) 95, 464, 519, 559, 768
AfaI GTAC 2 cut(s) 28, 844
AfiI CCNNNNNNNGG 2 cut(s) 628, 682
AflII CTTAAG 1 cut(s) 10
AgeI ACCGGT 1 cut(s) 251
AgsI TTSAA 3 cut(s) 725, 765, 851
AjiI CACGTC 1 cut(s) 603
AjnI CCWGG 2 cut(s) 485, 624
AluBI AGCT 3 cut(s) 552, 732, 940
AluI AGCT 3 cut(s) 552, 732, 940
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 2 cut(s) 11, 1060
AlwI GGATC 1 cut(s) 224
AoxI GGCC 3 cut(s) 421, 445, 473
ApoI RAATTY 5 cut(s) 95, 464, 519, 559, 768
AsiGI ACCGGT 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 992
AspLEI GCGC 1 cut(s) 1078
AspS9I GGNCC 3 cut(s) 421, 473, 631
AsuC2I CCSGG 1 cut(s) 908
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 642
BccI CCATC 3 cut(s) 34, 38, 412
BciT130I CCWGG 2 cut(s) 487, 626
BcnI CCSGG 1 cut(s) 908
BcoDI GTCTC 2 cut(s) 11, 1060
BfaI CTAG 5 cut(s) 437, 576, 597, 858, 864
BfmI CTRYAG 1 cut(s) 953
BfrI CTTAAG 1 cut(s) 10
BisI GCNGC 1 cut(s) 349
BlsI GCNGC 1 cut(s) 350
Bme1390I CCNGG 3 cut(s) 487, 626, 908
Bme18I GGWCC 1 cut(s) 631
BmgBI CACGTC 1 cut(s) 603
BmgT120I GGNCC 3 cut(s) 421, 473, 631
BmiI GGNNCC 3 cut(s) 222, 474, 1029
BmrFI CCNGG 3 cut(s) 487, 626, 908
BmsI GCATC 3 cut(s) 421, 745, 1003
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 647
Bpu10I CCTNAGC 2 cut(s) 695, 917
BpuMI CCSGG 1 cut(s) 908
BsaJI CCNNGG 5 cut(s) 335, 476, 801, 906, 1093
BsaWI WCCGGW 2 cut(s) 251, 274
BsaXI ACNNNNNCTCC 2 cut(s) 883, 913
Bsc4I CCNNNNNNNGG 2 cut(s) 628, 682
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 1037
Bse21I CCTNAGG 1 cut(s) 425
BseBI CCWGG 2 cut(s) 487, 626
BseDI CCNNGG 5 cut(s) 335, 476, 801, 906, 1093
BseGI GGATG 3 cut(s) 49, 169, 436
BseLI CCNNNNNNNGG 2 cut(s) 628, 682
BseMII CTCAG 4 cut(s) 305, 416, 468, 931
BseNI ACTGG 1 cut(s) 1037
BseRI GAGGAG 3 cut(s) 47, 50, 670
BseYI CCCAGC 1 cut(s) 732
Bsh1236I CGCG 2 cut(s) 793, 1095
BshFI GGCC 3 cut(s) 423, 447, 475
BshTI ACCGGT 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 3 cut(s) 252, 275, 908
BslI CCNNNNNNNGG 2 cut(s) 628, 682
BsmAI GTCTC 2 cut(s) 11, 1060
BsnI GGCC 3 cut(s) 423, 447, 475
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 4 cut(s) 3, 229, 352, 607
Bsp19I CCATGG 1 cut(s) 335
BspACI CCGC 6 cut(s) 348, 787, 793, 902, 1093, 1095
BspANI GGCC 3 cut(s) 423, 447, 475
BspCNI CTCAG 4 cut(s) 306, 417, 467, 930
BspFNI CGCG 2 cut(s) 793, 1095
BspLI GGNNCC 3 cut(s) 222, 474, 1029
BspPI GGATC 1 cut(s) 224
BspTI CTTAAG 1 cut(s) 10
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 1 cut(s) 1037
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 5 cut(s) 335, 476, 801, 906, 1093
BssMI GATC 4 cut(s) 3, 229, 352, 607
BssNAI GTATAC 1 cut(s) 483
BssT1I CCWWGG 2 cut(s) 335, 801
Bst1107I GTATAC 1 cut(s) 483
Bst2UI CCWGG 2 cut(s) 487, 626
Bst4CI ACNGT 6 cut(s) 116, 197, 240, 284, 1022, 1054
Bst6I CTCTTC 2 cut(s) 153, 696
BstAFI CTTAAG 1 cut(s) 10
BstC8I GCNNGC 5 cut(s) 713, 1046, 1080, 1084, 1088
BstDEI CTNAG 5 cut(s) 314, 425, 454, 695, 917
BstDSI CCRYGG 2 cut(s) 335, 1093
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 3 cut(s) 49, 169, 436
BstFNI CGCG 2 cut(s) 793, 1095
BstHHI GCGC 1 cut(s) 1078
BstKTI GATC 4 cut(s) 6, 232, 355, 610
BstMAI GTCTC 2 cut(s) 11, 1060
BstMBI GATC 4 cut(s) 3, 229, 352, 607
BstMWI GCNNNNNNNGC 2 cut(s) 869, 1092
BstNI CCWGG 2 cut(s) 487, 626
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 3 cut(s) 485, 624, 906
BstSFI CTRYAG 1 cut(s) 953
BstUI CGCG 2 cut(s) 793, 1095
BstV2I GAAGAC 1 cut(s) 61
BstXI CCANNNNNNTGG 1 cut(s) 240
BstZ17I GTATAC 1 cut(s) 483
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 3 cut(s) 423, 447, 475
BtgI CCRYGG 2 cut(s) 335, 1093
BtrI CACGTC 1 cut(s) 603
BtsCI GGATG 3 cut(s) 49, 169, 436
Cac8I GCNNGC 5 cut(s) 713, 1046, 1080, 1084, 1088
CfoI GCGC 1 cut(s) 1078
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 421, 473, 631
Cfr42I CCGCGG 1 cut(s) 1096
CseI GACGC 1 cut(s) 799
Csp6I GTAC 2 cut(s) 27, 843
CspAI ACCGGT 1 cut(s) 251
CviAII CATG 4 cut(s) 294, 323, 336, 643
CviQI GTAC 2 cut(s) 27, 843
DdeI CTNAG 5 cut(s) 314, 425, 454, 695, 917
DpnI GATC 4 cut(s) 5, 231, 354, 609
DpnII GATC 4 cut(s) 3, 229, 352, 607
DraI TTTAAA 1 cut(s) 591
DrdI GACNNNNNNGTC 1 cut(s) 536
DseDI GACNNNNNNGTC 1 cut(s) 536
Eam1104I CTCTTC 2 cut(s) 153, 696
EarI CTCTTC 2 cut(s) 153, 696
EciI GGCGGA 2 cut(s) 802, 891
Eco130I CCWWGG 2 cut(s) 335, 801
Eco47I GGWCC 1 cut(s) 631
Eco81I CCTNAGG 1 cut(s) 425
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoRII CCWGG 2 cut(s) 485, 624
EcoT14I CCWWGG 2 cut(s) 335, 801
ErhI CCWWGG 2 cut(s) 335, 801
FaeI CATG 4 cut(s) 297, 326, 339, 646
FatI CATG 4 cut(s) 293, 322, 335, 642
FauI CCCGC 1 cut(s) 1088
FblI GTMKAC 1 cut(s) 482
Fnu4HI GCNGC 1 cut(s) 349
FokI GGATG 3 cut(s) 56, 176, 443
Fsp4HI GCNGC 1 cut(s) 349
FspBI CTAG 5 cut(s) 437, 576, 597, 858, 864
FspI TGCGCA 1 cut(s) 1077
GlaI GCGC 1 cut(s) 1077
GluI GCNGC 1 cut(s) 349
GsaI CCCAGC 1 cut(s) 736
GsuI CTGGAG 1 cut(s) 647
HaeIII GGCC 3 cut(s) 423, 447, 475
HapII CCGG 3 cut(s) 252, 275, 908
HgaI GACGC 1 cut(s) 799
HhaI GCGC 1 cut(s) 1078
Hin1II CATG 4 cut(s) 297, 326, 339, 646
Hin6I GCGC 1 cut(s) 1076
HinP1I GCGC 1 cut(s) 1076
HinfI GANTC 5 cut(s) 19, 525, 672, 806, 832
HpaII CCGG 3 cut(s) 252, 275, 908
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 6 cut(s) 193, 229, 357, 457, 607, 754
Hpy8I GTNNAC 1 cut(s) 483
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 6 cut(s) 116, 197, 240, 284, 1022, 1054
HpyCH4IV ACGT 1 cut(s) 602
HpyCH4V TGCA 6 cut(s) 322, 434, 646, 711, 1048, 1102
HpyF10VI GCNNNNNNNGC 2 cut(s) 869, 1092
HpyF3I CTNAG 5 cut(s) 314, 425, 454, 695, 917
HpySE526I ACGT 1 cut(s) 602
Hsp92II CATG 4 cut(s) 297, 326, 339, 646
HspAI GCGC 1 cut(s) 1076
KspI CCGCGG 1 cut(s) 1096
Kzo9I GATC 4 cut(s) 3, 229, 352, 607
LmnI GCTCC 1 cut(s) 1033
LweI GCATC 3 cut(s) 421, 745, 1003
MaeI CTAG 5 cut(s) 437, 576, 597, 858, 864
MaeII ACGT 1 cut(s) 602
MalI GATC 4 cut(s) 5, 231, 354, 609
MboI GATC 4 cut(s) 3, 229, 352, 607
MboII GAAGA 9 cut(s) 66, 82, 170, 605, 681, 713, 863, 941, 1000
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 8 cut(s) 73, 95, 363, 464, 519, 559, 686, 768
MlyI GAGTC 3 cut(s) 13, 519, 841
MmeI TCCRAC 1 cut(s) 909
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 4 cut(s) 11, 306, 516, 590
MslI CAYNNNNRTG 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 1095
MspCI CTTAAG 1 cut(s) 10
MspI CCGG 3 cut(s) 252, 275, 908
MspR9I CCNGG 3 cut(s) 487, 626, 908
MvaI CCWGG 2 cut(s) 487, 626
MvnI CGCG 2 cut(s) 793, 1095
MwoI GCNNNNNNNGC 2 cut(s) 869, 1092
NciI CCSGG 1 cut(s) 908
NcoI CCATGG 1 cut(s) 335
NdeII GATC 4 cut(s) 3, 229, 352, 607
NlaIII CATG 4 cut(s) 297, 326, 339, 646
NlaIV GGNNCC 3 cut(s) 222, 474, 1029
NmeAIII GCCGAG 1 cut(s) 501
NsbI TGCGCA 1 cut(s) 1077
NspI RCATGY 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 2 cut(s) 672, 806
PfoI TCCNGGA 1 cut(s) 624
PinAI ACCGGT 1 cut(s) 251
PkrI GCNGC 1 cut(s) 350
PleI GAGTC 3 cut(s) 13, 519, 840
PpsI GAGTC 3 cut(s) 13, 519, 840
Psp6I CCWGG 2 cut(s) 485, 624
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 2 cut(s) 485, 624
PspN4I GGNNCC 3 cut(s) 222, 474, 1029
PspPI GGNCC 3 cut(s) 421, 473, 631
RsaI GTAC 2 cut(s) 28, 844
RsaNI GTAC 2 cut(s) 27, 843
RseI CAYNNNNRTG 1 cut(s) 204
SacII CCGCGG 1 cut(s) 1096
SaqAI TTAA 4 cut(s) 11, 306, 516, 590
SatI GCNGC 1 cut(s) 349
Sau3AI GATC 4 cut(s) 3, 229, 352, 607
Sau96I GGNCC 3 cut(s) 421, 473, 631
SchI GAGTC 3 cut(s) 13, 519, 841
ScrFI CCNGG 3 cut(s) 487, 626, 908
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 3 cut(s) 421, 745, 1003
SfcI CTRYAG 1 cut(s) 953
Sfr303I CCGCGG 1 cut(s) 1096
SgrBI CCGCGG 1 cut(s) 1096
SinI GGWCC 1 cut(s) 631
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 8 cut(s) 73, 95, 363, 464, 519, 559, 686, 768
SsiI CCGC 6 cut(s) 348, 787, 793, 902, 1093, 1095
SspMI CTAG 5 cut(s) 437, 576, 597, 858, 864
StyD4I CCNGG 3 cut(s) 485, 624, 906
StyI CCWWGG 2 cut(s) 335, 801
TaaI ACNGT 6 cut(s) 116, 197, 240, 284, 1022, 1054
TaiI ACGT 1 cut(s) 605
TaqI TCGA 4 cut(s) 22, 371, 523, 528
TasI AATT 8 cut(s) 73, 95, 363, 464, 519, 559, 686, 768
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 2 cut(s) 672, 806
Tru1I TTAA 4 cut(s) 11, 306, 516, 590
Tru9I TTAA 4 cut(s) 11, 306, 516, 590
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 3 cut(s) 44, 623, 665
Vha464I CTTAAG 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 631
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 5 cut(s) 95, 464, 519, 559, 768
XceI RCATGY 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 5 cut(s) 437, 576, 597, 858, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.