FvH4_6g00150

ycf20-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011386
Physical Location & Seq
Forward (+)
122434 .. 125072
2639 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00150.t1

Sequence Viewer

Length: 627 bp
ATGGGTACCATGATGCTACAGTCAACTTTTCCAATTCGGGAGAAAAAGATCCATGAAAGCTCGTTTTTTACTCTTGCAACTGGTCTAGTCCAGAACTGTTGCTGTGAGCCGAGTTTCAGTATGCAGACATCAAAGATTGGCTTTAACTCTGTGCCTTCTTTTCCGCAAAGGAGATTTCAGTCAAGAAAGCATCGATTGGGATTAGCCTTTGCCTTGGACACAGGTGGGGTTCCTGATAATGGTGGCCAAGAGAGCGTCAATGACAACAACTTCAATGGTCTCAATCGCACTCGTTTAGGTCGGATAGTGACTGCAGCCGGAAAACAGCTATTAGAGAAGCTGAACTTAGCTAGAAAAAACTTCCCCATGAAGATATTTCTACTTCTGTTGGGTTTCTACACAGCAAATGCATTGGCCACAATCCTTGGGCAGACGGGAGATTGGGATGTCTTGGTTGCAGGAATTGTGGTTGCTGCCATTGAAGGTATTGGTATGCTTATGTATAGAAAGTCCACTTCCCCTTCTTTATCAAGTGGGAAGCTCAAGTCTTTTGTCATGCTGATGAATTACTGGAAAGCTGGTGTGTGCTTAGGCCTCTTTGTGGATGCTTTTAAATTGGGTAGTTAG

Protein Analysis

209

Amino Acids

22.76

Weight (kDa)

9.83

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 144 - 208 1.9e-22 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011386)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65420
fragaria_vesca FvH4_6g00150 FvH4_6g00150 FvH4_6g00150
malus_domestica MD04G1247900.v1.1 MD12G1262600.v1.1
prunus_persica Prupe.6G364400_v2.0.a1 Prupe.6G364400_v2.0.a1 Prupe.6G364400_v2.0.a1
pyrus_communis pycom04g21860
rosa_chinensis RchiOBHm_Chr3g0447501
rosa_laevigata RLG00000025992
rosa_multiflora Rmu_sc0006572.1_g000003
rosa_roxburghii Rroxscaffold_6G00425720
rosa_rugosa Rorug02G0602400
rosa_samantha Rh3AG002600 Rh3BG002100 Rh3CG002400 Rh3DG002500
rosa_wichuraiana Rw3G000150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AciI CCGC 1 cut(s) 164
AclWI GGATC 1 cut(s) 43
AcoI YGGCCR 2 cut(s) 244, 414
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 239, 601
AgsI TTSAA 2 cut(s) 274, 482
AluBI AGCT 6 cut(s) 60, 328, 340, 350, 541, 578
AluI AGCT 6 cut(s) 60, 328, 340, 350, 541, 578
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 1 cut(s) 43
AoxI GGCC 3 cut(s) 244, 414, 592
ApeKI GCWGC 2 cut(s) 314, 473
Asp718I GGTACC 1 cut(s) 5
BalI TGGCCA 2 cut(s) 246, 416
BanI GGYRCC 1 cut(s) 5
BbvI GCAGC 2 cut(s) 326, 460
BcoDI GTCTC 1 cut(s) 284
BfaI CTAG 2 cut(s) 86, 351
BfmI CTRYAG 2 cut(s) 17, 312
BisI GCNGC 2 cut(s) 315, 474
BlsI GCNGC 2 cut(s) 316, 475
BmiI GGNNCC 2 cut(s) 7, 231
BmsI GCATC 3 cut(s) 3, 199, 595
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 1 cut(s) 527
Bsa29I ATCGAT 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 213, 424
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 601
Bse1I ACTGG 2 cut(s) 85, 575
BseCI ATCGAT 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 213, 424
BseGI GGATG 2 cut(s) 451, 610
BseLI CCNNNNNNNGG 2 cut(s) 239, 601
BseNI ACTGG 2 cut(s) 85, 575
BseXI GCAGC 2 cut(s) 326, 460
BshFI GGCC 3 cut(s) 246, 416, 594
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 193
BsiSI CCGG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 239, 601
BsmAI GTCTC 1 cut(s) 284
BsnI GGCC 3 cut(s) 246, 416, 594
Bso31I GGTCTC 1 cut(s) 284
Bsp143I GATC 1 cut(s) 48
BspACI CCGC 1 cut(s) 164
BspANI GGCC 3 cut(s) 246, 416, 594
BspDI ATCGAT 1 cut(s) 193
BspLI GGNNCC 2 cut(s) 7, 231
BspMAI CTGCAG 1 cut(s) 316
BspPI GGATC 1 cut(s) 43
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 1 cut(s) 284
BsrI ACTGG 2 cut(s) 85, 575
BssECI CCNNGG 2 cut(s) 213, 424
BssMI GATC 1 cut(s) 48
BssT1I CCWWGG 2 cut(s) 213, 424
Bst4CI ACNGT 2 cut(s) 21, 98
BstDEI CTNAG 2 cut(s) 346, 589
BstF5I GGATG 2 cut(s) 451, 610
BstKTI GATC 1 cut(s) 51
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 1 cut(s) 48
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstSFI CTRYAG 2 cut(s) 17, 312
BstV1I GCAGC 2 cut(s) 326, 460
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
Bsu15I ATCGAT 1 cut(s) 193
BsuRI GGCC 3 cut(s) 246, 416, 594
BsuTUI ATCGAT 1 cut(s) 193
BtsCI GGATG 2 cut(s) 451, 610
ClaI ATCGAT 1 cut(s) 193
CseI GACGC 1 cut(s) 244
Csp6I GTAC 1 cut(s) 6
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 4 cut(s) 10, 53, 367, 556
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 2 cut(s) 346, 589
DpnI GATC 1 cut(s) 50
DpnII GATC 1 cut(s) 48
DraI TTTAAA 1 cut(s) 613
EaeI YGGCCR 2 cut(s) 244, 414
Eco130I CCWWGG 2 cut(s) 213, 424
Eco147I AGGCCT 1 cut(s) 594
Eco31I GGTCTC 1 cut(s) 284
EcoT14I CCWWGG 2 cut(s) 213, 424
EcoT22I ATGCAT 1 cut(s) 412
ErhI CCWWGG 2 cut(s) 213, 424
FaeI CATG 4 cut(s) 13, 56, 370, 559
FaiI YATR 8 cut(s) 11, 54, 122, 368, 494, 500, 504, 557
FalI AAGNNNNNCTT 4 cut(s) 125, 157, 329, 361
FatI CATG 4 cut(s) 9, 52, 366, 555
Fnu4HI GCNGC 2 cut(s) 315, 474
FokI GGATG 2 cut(s) 458, 617
Fsp4HI GCNGC 2 cut(s) 315, 474
FspBI CTAG 2 cut(s) 86, 351
GluI GCNGC 2 cut(s) 315, 474
HaeIII GGCC 3 cut(s) 246, 416, 594
HapII CCGG 1 cut(s) 318
HgaI GACGC 1 cut(s) 244
Hin1II CATG 4 cut(s) 13, 56, 370, 559
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HpaII CCGG 1 cut(s) 318
Hpy166II GTNNAC 2 cut(s) 24, 513
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 4 cut(s) 38, 91, 183, 233
Hpy8I GTNNAC 2 cut(s) 24, 513
HpyAV CCTTC 3 cut(s) 165, 476, 531
HpyCH4III ACNGT 2 cut(s) 21, 98
HpyCH4V TGCA 5 cut(s) 77, 124, 314, 410, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
HpyF3I CTNAG 2 cut(s) 346, 589
Hsp92II CATG 4 cut(s) 13, 56, 370, 559
KpnI GGTACC 1 cut(s) 9
Kzo9I GATC 1 cut(s) 48
LpnPI CCDG 8 cut(s) 66, 104, 207, 246, 331, 444, 556, 564
Lsp1109I GCAGC 2 cut(s) 326, 460
LweI GCATC 3 cut(s) 3, 199, 595
MaeI CTAG 2 cut(s) 86, 351
MaeIII GTNAC 1 cut(s) 307
MalI GATC 1 cut(s) 50
MboI GATC 1 cut(s) 48
MboII GAAGA 1 cut(s) 382
MflI RGATCY 1 cut(s) 48
MlsI TGGCCA 2 cut(s) 246, 416
MluCI AATT 4 cut(s) 33, 462, 565, 614
MluNI TGGCCA 2 cut(s) 246, 416
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 1 cut(s) 605
Mox20I TGGCCA 2 cut(s) 246, 416
Mph1103I ATGCAT 1 cut(s) 412
MscI TGGCCA 2 cut(s) 246, 416
MseI TTAA 2 cut(s) 144, 612
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 2 cut(s) 246, 416
MspI CCGG 1 cut(s) 318
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeII GATC 1 cut(s) 48
NlaIII CATG 4 cut(s) 13, 56, 370, 559
NlaIV GGNNCC 2 cut(s) 7, 231
NmeAIII GCCGAG 1 cut(s) 135
NmuCI GTSAC 1 cut(s) 307
NsiI ATGCAT 1 cut(s) 412
PceI AGGCCT 1 cut(s) 594
PkrI GCNGC 2 cut(s) 316, 475
PspN4I GGNNCC 2 cut(s) 7, 231
PstI CTGCAG 1 cut(s) 316
PsuI RGATCY 1 cut(s) 48
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 560
SaqAI TTAA 2 cut(s) 144, 612
SatI GCNGC 2 cut(s) 315, 474
Sau3AI GATC 1 cut(s) 48
SetI ASST 9 cut(s) 62, 226, 301, 330, 342, 352, 487, 543, 580
SfaNI GCATC 3 cut(s) 3, 199, 595
SfcI CTRYAG 2 cut(s) 17, 312
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 4 cut(s) 33, 462, 565, 614
SseBI AGGCCT 1 cut(s) 594
SsiI CCGC 1 cut(s) 164
SspMI CTAG 2 cut(s) 86, 351
StuI AGGCCT 1 cut(s) 594
StyI CCWWGG 2 cut(s) 213, 424
TaaI ACNGT 2 cut(s) 21, 98
TaqI TCGA 1 cut(s) 193
TasI AATT 4 cut(s) 33, 462, 565, 614
Tru1I TTAA 2 cut(s) 144, 612
Tru9I TTAA 2 cut(s) 144, 612
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 314, 473
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 69, 383, 578
XspI CTAG 2 cut(s) 86, 351
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.