AT1G66550
WRKY Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0020198
Physical Location & Seq
Forward (+)
24828502 .. 24829660
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66550.1

Sequence Viewer

Length: 765 bp
ATGGTTTCCAACATTGATCACAAGGCTATGGAAGCACTCCTCCGTGGCCAAGGATGCGCTAACAACCTCAAGATTCTCCTTGAAAACGGCGAAATAAGCTCAGTTTCAACAGAACCACTCATCCACACCATTCTCGATTCTTTCTCACTTGCTCTGTCTTTTATGGATTCTCCTAATCATCCACCATACCATGAATCCTCTTCTCATAACATGGCAAGTCATATGTCCCGGAGATCATCTAAGCAAGTACAACATCGCAGAAAACTTTGTGTAGCAGAAGGTTTAGTGAATTACAATCACGATTCCCGGACTATGTGCCCCAATGATGGCTTCACCTGGAGGAAATATGGACAAAAAACCATTAAAGCCTCAGCGCACAAAAGGTGTTACTATCGGTGTACCTATGCAAAAGACCAAAACTGCAATGCTACAAAGCGGGTGCAGAAGATCAAAGACAACCCTCCAGTGTACAGAACCACTTACTTGGGAAAACATGTGTGTAAAGCTTTTGCAGTTCATGATGATACATATAGTTCCACGATGATTCGATTCGACCAAGTTGTTCCTGAACCGATTATGCCGCAGCTCACAACAATTGACCACCAAGTAATTACCGTGGAGGAAAACTCCGCAGAACATATCATGAACCAAGAATGTGATATTAATGATTATTTGGTGGATGATGACCCATTTTGGGCTAGTCAATTTCCCCCGTTTCCATCGAGTGACACAATGTTCTTGGAAAACATTTCTGCTTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

254

Amino Acids

29.04

Weight (kDa)

6.34

Isoelectric Point (pI)

55.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 108 - 168 1.1e-21 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020198)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66550 AT1G66550 AT1G66560 AT1G66600 AT1G80590 AT5G01900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 436, 581, 630
AcoI YGGCCR 1 cut(s) 46
AfaI GTAC 3 cut(s) 249, 400, 470
AfiI CCNNNNNNNGG 2 cut(s) 326, 694
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 2 cut(s) 83, 108
AjnI CCWGG 1 cut(s) 335
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 3 cut(s) 99, 506, 586
AluI AGCT 3 cut(s) 99, 506, 586
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 583
AseI ATTAAT 1 cut(s) 663
AspLEI GCGC 2 cut(s) 59, 376
AsuC2I CCSGG 2 cut(s) 229, 307
AsuHPI GGTGA 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 320
BalI TGGCCA 1 cut(s) 48
BbvCI CCTCAGC 1 cut(s) 370
BbvI GCAGC 1 cut(s) 595
BccI CCATC 2 cut(s) 320, 727
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 337
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 2 cut(s) 229, 307
BfaI CTAG 1 cut(s) 699
BisI GCNGC 2 cut(s) 581, 584
BlsI GCNGC 2 cut(s) 582, 585
Bme1390I CCNGG 3 cut(s) 229, 307, 337
BmrFI CCNGG 3 cut(s) 229, 307, 337
BmsI GCATC 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpmI CTGGAG 2 cut(s) 358, 447
Bpu10I CCTNAGC 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 2 cut(s) 229, 307
BsaJI CCNNGG 3 cut(s) 43, 49, 615
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 694
Bse1I ACTGG 1 cut(s) 464
Bse3DI GCAATG 1 cut(s) 430
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 43, 49, 615
BseGI GGATG 4 cut(s) 59, 120, 178, 685
BseLI CCNNNNNNNGG 2 cut(s) 326, 694
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 2 cut(s) 114, 384
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 29
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 1 cut(s) 595
BsgI GTGCAG 1 cut(s) 461
BshFI GGCC 1 cut(s) 48
BsiSI CCGG 2 cut(s) 229, 307
BslFI GGGAC 1 cut(s) 211
BslI CCNNNNNNNGG 2 cut(s) 326, 694
BsmFI GGGAC 1 cut(s) 211
BsnI GGCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 320
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 3 cut(s) 16, 233, 447
BspACI CCGC 3 cut(s) 436, 581, 630
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 2 cut(s) 113, 383
BspHI TCATGA 2 cut(s) 517, 642
BsrDI GCAATG 1 cut(s) 430
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 1 cut(s) 464
BssECI CCNNGG 3 cut(s) 43, 49, 615
BssMI GATC 3 cut(s) 16, 233, 447
BssT1I CCWWGG 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 1 cut(s) 205
BstAUI TGTACA 1 cut(s) 468
BstDEI CTNAG 3 cut(s) 100, 240, 370
BstDSI CCRYGG 2 cut(s) 43, 615
BstF5I GGATG 4 cut(s) 59, 120, 178, 685
BstHHI GCGC 2 cut(s) 59, 376
BstKTI GATC 3 cut(s) 19, 236, 450
BstMBI GATC 3 cut(s) 16, 233, 447
BstMWI GCNNNNNNNGC 3 cut(s) 32, 54, 96
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 3 cut(s) 227, 305, 335
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 595
BstXI CCANNNNNNTGG 1 cut(s) 484
BsuRI GGCC 1 cut(s) 48
BtgI CCRYGG 2 cut(s) 43, 615
BtgZI GCGATG 1 cut(s) 239
BtsCI GGATG 4 cut(s) 59, 120, 178, 685
BtsIMutI CAGTG 1 cut(s) 471
CciI TCATGA 2 cut(s) 517, 642
CfoI GCGC 2 cut(s) 59, 376
Csp6I GTAC 3 cut(s) 248, 399, 469
CviAII CATG 5 cut(s) 191, 211, 494, 518, 643
CviJI RGCY 8 cut(s) 26, 48, 99, 330, 368, 506, 586, 698
CviKI_1 RGCY 8 cut(s) 26, 48, 99, 330, 368, 506, 586, 698
CviQI GTAC 3 cut(s) 248, 399, 469
DdeI CTNAG 3 cut(s) 100, 240, 370
DpnI GATC 3 cut(s) 18, 235, 449
DpnII GATC 3 cut(s) 16, 233, 447
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 5 cut(s) 194, 214, 497, 521, 646
FaqI GGGAC 1 cut(s) 211
FatI CATG 5 cut(s) 190, 210, 493, 517, 642
FauI CCCGC 1 cut(s) 429
FauNDI CATATG 1 cut(s) 222
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 581, 584
FokI GGATG 4 cut(s) 66, 107, 165, 692
Fsp4HI GCNGC 2 cut(s) 581, 584
FspBI CTAG 1 cut(s) 699
GlaI GCGC 2 cut(s) 58, 375
GluI GCNGC 2 cut(s) 581, 584
GsuI CTGGAG 2 cut(s) 358, 447
HaeIII GGCC 1 cut(s) 48
HapII CCGG 2 cut(s) 229, 307
HhaI GCGC 2 cut(s) 59, 376
Hin1II CATG 5 cut(s) 194, 214, 497, 521, 646
Hin6I GCGC 2 cut(s) 57, 374
HinP1I GCGC 2 cut(s) 57, 374
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 7 cut(s) 73, 137, 167, 194, 302, 544, 549
HpaII CCGG 2 cut(s) 229, 307
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 2 cut(s) 399, 469
Hpy188III TCNNGA 6 cut(s) 70, 134, 299, 518, 566, 643
Hpy8I GTNNAC 2 cut(s) 399, 469
HpyAV CCTTC 1 cut(s) 272
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4V TGCA 4 cut(s) 407, 423, 442, 512
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 54, 96
HpyF3I CTNAG 3 cut(s) 100, 240, 370
Hsp92II CATG 5 cut(s) 194, 214, 497, 521, 646
HspAI GCGC 2 cut(s) 57, 374
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 3 cut(s) 16, 233, 447
LpnPI CCDG 6 cut(s) 242, 320, 322, 349, 477, 579
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 1 cut(s) 44
MaeI CTAG 1 cut(s) 699
MaeIII GTNAC 2 cut(s) 386, 725
MalI GATC 3 cut(s) 18, 235, 449
MboI GATC 3 cut(s) 16, 233, 447
MboII GAAGA 2 cut(s) 192, 457
MfeI CAATTG 1 cut(s) 594
MhlI GDGCHC 1 cut(s) 320
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 4 cut(s) 289, 594, 609, 704
MluNI TGGCCA 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 7 cut(s) 50, 77, 208, 333, 379, 471, 613
Mox20I TGGCCA 1 cut(s) 48
MscI TGGCCA 1 cut(s) 48
MseI TTAA 2 cut(s) 363, 663
Msp20I TGGCCA 1 cut(s) 48
MspI CCGG 2 cut(s) 229, 307
MspR9I CCNGG 3 cut(s) 229, 307, 337
MunI CAATTG 1 cut(s) 594
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 3 cut(s) 32, 54, 96
NciI CCSGG 2 cut(s) 229, 307
NdeI CATATG 1 cut(s) 222
NdeII GATC 3 cut(s) 16, 233, 447
NlaIII CATG 5 cut(s) 194, 214, 497, 521, 646
NmuCI GTSAC 1 cut(s) 725
NspI RCATGY 1 cut(s) 497
PagI TCATGA 2 cut(s) 517, 642
PciI ACATGT 1 cut(s) 493
PfeI GAWTC 7 cut(s) 73, 137, 167, 194, 302, 544, 549
PfoI TCCNGGA 2 cut(s) 227, 305
PkrI GCNGC 2 cut(s) 582, 585
PscI ACATGT 1 cut(s) 493
PshBI ATTAAT 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
RsaI GTAC 3 cut(s) 249, 400, 470
RsaNI GTAC 3 cut(s) 248, 399, 469
SaqAI TTAA 2 cut(s) 363, 663
SatI GCNGC 2 cut(s) 581, 584
Sau3AI GATC 3 cut(s) 16, 233, 447
ScrFI CCNGG 3 cut(s) 229, 307, 337
SduI GDGCHC 1 cut(s) 320
SetI ASST 8 cut(s) 69, 101, 283, 338, 386, 404, 508, 588
SfaNI GCATC 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 4 cut(s) 289, 594, 609, 704
SsiI CCGC 3 cut(s) 436, 581, 630
SspMI CTAG 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 227, 305, 335
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 616
TaqI TCGA 4 cut(s) 135, 547, 552, 722
TasI AATT 4 cut(s) 289, 594, 609, 704
TatI WGTACW 2 cut(s) 247, 468
TauI GCSGC 1 cut(s) 583
TfiI GAWTC 7 cut(s) 73, 137, 167, 194, 302, 544, 549
Tru1I TTAA 2 cut(s) 363, 663
Tru9I TTAA 2 cut(s) 363, 663
TscAI CASTG 1 cut(s) 471
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 1 cut(s) 583
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 3 cut(s) 207, 506, 659
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 1 cut(s) 471
VspI ATTAAT 1 cut(s) 663
XceI RCATGY 1 cut(s) 497
XspI CTAG 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.