AT5G01900
WRKY Family

DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020198
Physical Location & Seq
Reverse (-)
350925 .. 352095
1171 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01900.1

Sequence Viewer

Length: 792 bp
ATGAACTCTTGCCAACAAAAGGCTATGGAGAAGTTGCTTCACGGGCATGGGTGCGCCAACCAGCTGCTCATCATGGACCAGACCGAGTCAGACTCGTCCATGGAAAGGGAGGATTTGGCCAAGTCCGTCCTCCATTGTTTCTCAGATGCGCTCTCCATCTTGATCGATACTAATGACCACCAAGACGATCAATCCAATAACTCATCTCCTCAGGACTCTTCTCCTGTGCTTGAGAGCTCCAGGAAACCACTTCACAAGAGGGGAAGAAAGACATCTATGGCAGAGAGCTCAGACTACCATAGACACGAATCTTCAACCCCGATCTACCACGACGGCTTCCTCTGGAGGAAATACGGACAAAAGCAGATCAAAGAATCTGAGTACCAAAGGAGTTACTACAAGTGTGCCTACACCAAGGACCAGAACTGTGAAGCAAAGAAGCAGGTTCAAAAGATCCAACACAATCCTCCATTGTACTCAACCACTTACTTCGGCCAGCACATATGCCAGCTTCATCAAGCTTATGCAACTTTCCCCATTGACACATCTGACTTCGAAGAGCACGAGGGATCCCACATGATCCGATTCGGTCACCCCAACATCAGCTTCTCCTCCTCCACCTCTAACCTCCGCCAACATCAGAATCATCAGGATCGCATCAAAGATGAATACATGAAACCGGTGATAGCAGAAGATTGGTCTCCATCCCAGTGGATGTCGTCCGAGGTTGCCCTCGCCGTGGAGGCTTTCGAGTTTAATCCTTTCTGGACATCTCACGACTTATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

263

Amino Acids

30.44

Weight (kDa)

5.91

Isoelectric Point (pI)

57.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 110 - 170 1.3e-22 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020198)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66550 AT1G66550 AT1G66560 AT1G66600 AT1G80590 AT5G01900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 433
AciI CCGC 1 cut(s) 631
AclWI GGATC 5 cut(s) 448, 564, 574, 577, 660
AcoI YGGCCR 2 cut(s) 117, 493
AfaI GTAC 2 cut(s) 383, 476
AfiI CCNNNNNNNGG 3 cut(s) 19, 105, 739
AgeI ACCGGT 1 cut(s) 679
AgsI TTSAA 2 cut(s) 315, 449
AjnI CCWGG 1 cut(s) 239
AjuI GAANNNNNNNTTGG 2 cut(s) 590, 622
AluBI AGCT 6 cut(s) 64, 237, 288, 511, 521, 606
AluI AGCT 6 cut(s) 64, 237, 288, 511, 521, 606
Alw21I GWGCWC 3 cut(s) 239, 290, 564
Alw26I GTCTC 1 cut(s) 705
AlwI GGATC 5 cut(s) 448, 564, 574, 577, 660
AoxI GGCC 2 cut(s) 117, 493
ApeKI GCWGC 1 cut(s) 64
AsiGI ACCGGT 1 cut(s) 679
AspLEI GCGC 2 cut(s) 56, 151
AspS9I GGNCC 2 cut(s) 76, 418
AsuHPI GGTGA 2 cut(s) 584, 694
AsuII TTCGAA 1 cut(s) 555
AvaII GGWCC 2 cut(s) 76, 418
AxyI CCTNAGG 1 cut(s) 210
BalI TGGCCA 1 cut(s) 119
BamHI GGATCC 1 cut(s) 569
BanII GRGCYC 2 cut(s) 239, 290
BauI CACGAG 1 cut(s) 563
Bbv12I GWGCWC 3 cut(s) 239, 290, 564
BbvI GCAGC 1 cut(s) 51
BccI CCATC 2 cut(s) 164, 712
BceAI ACGGC 2 cut(s) 349, 722
BciT130I CCWGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 705
BfuAI ACCTGC 1 cut(s) 433
BglI GCCNNNNNGGC 1 cut(s) 743
BisI GCNGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 66
Bme1390I CCNGG 1 cut(s) 241
Bme18I GGWCC 2 cut(s) 76, 418
BmgT120I GGNCC 2 cut(s) 76, 418
BmiI GGNNCC 1 cut(s) 571
BmrFI CCNGG 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 703
BmsI GCATC 2 cut(s) 136, 666
BmuI ACTGGG 1 cut(s) 703
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpmI CTGGAG 2 cut(s) 223, 364
Bpu14I TTCGAA 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 251
Bsa29I ATCGAT 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 4 cut(s) 99, 414, 723, 738
BsaWI WCCGGW 1 cut(s) 679
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 105, 739
Bse118I RCCGGY 1 cut(s) 679
Bse1I ACTGG 1 cut(s) 709
Bse21I CCTNAGG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 241
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 4 cut(s) 99, 414, 723, 738
BseGI GGATG 2 cut(s) 704, 720
BseLI CCNNNNNNNGG 3 cut(s) 19, 105, 739
BseMII CTCAG 4 cut(s) 156, 224, 303, 369
BseNI ACTGG 1 cut(s) 709
BseRI GAGGAG 3 cut(s) 198, 601, 604
BseXI GCAGC 1 cut(s) 51
BshFI GGCC 2 cut(s) 119, 495
BshTI ACCGGT 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 165
BsiHKAI GWGCWC 3 cut(s) 239, 290, 564
BsiSI CCGG 1 cut(s) 680
BslI CCNNNNNNNGG 3 cut(s) 19, 105, 739
BsmAI GTCTC 1 cut(s) 705
BsnI GGCC 2 cut(s) 119, 495
Bso31I GGTCTC 1 cut(s) 705
Bsp119I TTCGAA 1 cut(s) 555
Bsp1286I GDGCHC 3 cut(s) 239, 290, 564
Bsp143I GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 1 cut(s) 631
BspANI GGCC 2 cut(s) 119, 495
BspCNI CTCAG 4 cut(s) 155, 223, 302, 370
BspDI ATCGAT 1 cut(s) 165
BspHI TCATGA 1 cut(s) 788
BspLI GGNNCC 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 5 cut(s) 448, 564, 574, 577, 660
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 555
BspTNI GGTCTC 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 679
BsrI ACTGG 1 cut(s) 709
BssAI RCCGGY 1 cut(s) 679
BssECI CCNNGG 4 cut(s) 99, 414, 723, 738
BssMI GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
BssSI CACGAG 1 cut(s) 563
BssT1I CCWWGG 2 cut(s) 99, 414
Bst2BI CACGAG 1 cut(s) 563
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 428
Bst6I CTCTTC 2 cut(s) 223, 552
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 2 cut(s) 497, 509
BstDEI CTNAG 4 cut(s) 142, 210, 289, 378
BstDSI CCRYGG 2 cut(s) 99, 738
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 2 cut(s) 704, 720
BstHHI GCGC 2 cut(s) 56, 151
BstKTI GATC 8 cut(s) 165, 190, 324, 369, 456, 572, 582, 655
BstMAI GTCTC 1 cut(s) 705
BstMBI GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
BstMWI GCNNNNNNNGC 2 cut(s) 43, 743
BstNI CCWGG 1 cut(s) 241
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 1 cut(s) 239
BstV1I GCAGC 1 cut(s) 51
BstX2I RGATCY 2 cut(s) 453, 569
BstXI CCANNNNNNTGG 1 cut(s) 711
BstYI RGATCY 2 cut(s) 453, 569
Bsu15I ATCGAT 1 cut(s) 165
Bsu36I CCTNAGG 1 cut(s) 210
BsuRI GGCC 2 cut(s) 119, 495
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 2 cut(s) 99, 738
BtsCI GGATG 2 cut(s) 704, 720
BtsIMutI CAGTG 1 cut(s) 716
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 2 cut(s) 497, 509
CciI TCATGA 1 cut(s) 788
CfoI GCGC 2 cut(s) 56, 151
Cfr10I RCCGGY 1 cut(s) 679
Cfr13I GGNCC 2 cut(s) 76, 418
ClaI ATCGAT 1 cut(s) 165
Csp6I GTAC 2 cut(s) 382, 475
CspAI ACCGGT 1 cut(s) 679
CviAII CATG 6 cut(s) 47, 73, 100, 577, 673, 789
CviQI GTAC 2 cut(s) 382, 475
DdeI CTNAG 4 cut(s) 142, 210, 289, 378
DpnI GATC 8 cut(s) 164, 189, 323, 368, 455, 571, 581, 654
DpnII GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
EaeI YGGCCR 2 cut(s) 117, 493
Eam1104I CTCTTC 2 cut(s) 223, 552
EarI CTCTTC 2 cut(s) 223, 552
EciI GGCGGA 1 cut(s) 620
Ecl136II GAGCTC 2 cut(s) 237, 288
Eco130I CCWWGG 2 cut(s) 99, 414
Eco24I GRGCYC 2 cut(s) 239, 290
Eco31I GGTCTC 1 cut(s) 705
Eco47I GGWCC 2 cut(s) 76, 418
Eco53kI GAGCTC 2 cut(s) 237, 288
Eco81I CCTNAGG 1 cut(s) 210
Eco91I GGTNACC 1 cut(s) 590
EcoICRI GAGCTC 2 cut(s) 237, 288
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 239
EcoT14I CCWWGG 2 cut(s) 99, 414
EcoT38I GRGCYC 2 cut(s) 239, 290
ErhI CCWWGG 2 cut(s) 99, 414
FaeI CATG 6 cut(s) 50, 76, 103, 580, 676, 792
FatI CATG 6 cut(s) 46, 72, 99, 576, 672, 788
FauNDI CATATG 1 cut(s) 503
Fnu4HI GCNGC 1 cut(s) 65
FokI GGATG 2 cut(s) 691, 727
FriOI GRGCYC 2 cut(s) 239, 290
Fsp4HI GCNGC 1 cut(s) 65
GlaI GCGC 2 cut(s) 55, 150
GluI GCNGC 1 cut(s) 65
GsuI CTGGAG 2 cut(s) 223, 364
HaeIII GGCC 2 cut(s) 119, 495
HapII CCGG 1 cut(s) 680
HhaI GCGC 2 cut(s) 56, 151
Hin1II CATG 6 cut(s) 50, 76, 103, 580, 676, 792
Hin6I GCGC 2 cut(s) 54, 149
HinP1I GCGC 2 cut(s) 54, 149
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 7 cut(s) 86, 92, 215, 308, 374, 585, 643
HpaII CCGG 1 cut(s) 680
HphI GGTGA 2 cut(s) 584, 694
Hpy188I TCNGA 8 cut(s) 91, 145, 292, 379, 550, 584, 642, 724
Hpy188III TCNNGA 7 cut(s) 160, 212, 343, 650, 766, 776, 789
Hpy99I CGWCG 1 cut(s) 335
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4V TGCA 1 cut(s) 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 743
HpyF3I CTNAG 4 cut(s) 142, 210, 289, 378
Hsp92II CATG 6 cut(s) 50, 76, 103, 580, 676, 792
HspAI GCGC 2 cut(s) 54, 149
Kzo9I GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 1 cut(s) 51
LweI GCATC 2 cut(s) 136, 666
MaeIII GTNAC 2 cut(s) 392, 590
MalI GATC 8 cut(s) 164, 189, 323, 368, 455, 571, 581, 654
MboI GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
MboII GAAGA 5 cut(s) 210, 276, 303, 569, 704
MflI RGATCY 2 cut(s) 453, 569
MhlI GDGCHC 3 cut(s) 239, 290, 564
MlsI TGGCCA 1 cut(s) 119
MluNI TGGCCA 1 cut(s) 119
MlyI GAGTC 3 cut(s) 86, 95, 209
MmeI TCCRAC 1 cut(s) 481
Mox20I TGGCCA 1 cut(s) 119
MscI TGGCCA 1 cut(s) 119
MseI TTAA 1 cut(s) 756
MslI CAYNNNNRTG 2 cut(s) 45, 709
Msp20I TGGCCA 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 1 cut(s) 680
MspR9I CCNGG 1 cut(s) 241
MvaI CCWGG 1 cut(s) 241
MwoI GCNNNNNNNGC 2 cut(s) 43, 743
NcoI CCATGG 1 cut(s) 99
NdeI CATATG 1 cut(s) 503
NdeII GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
NlaIII CATG 6 cut(s) 50, 76, 103, 580, 676, 792
NlaIV GGNNCC 1 cut(s) 571
NmuCI GTSAC 1 cut(s) 590
NspV TTCGAA 1 cut(s) 555
PagI TCATGA 1 cut(s) 788
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 561
PfeI GAWTC 4 cut(s) 308, 374, 585, 643
PflFI GACNNNGTC 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 239
PinAI ACCGGT 1 cut(s) 679
PkrI GCNGC 1 cut(s) 66
PleI GAGTC 3 cut(s) 86, 94, 209
PpsI GAGTC 3 cut(s) 86, 94, 209
Psp124BI GAGCTC 2 cut(s) 239, 290
Psp6I CCWGG 1 cut(s) 239
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 571
PspPI GGNCC 2 cut(s) 76, 418
PsuI RGATCY 2 cut(s) 453, 569
PsyI GACNNNGTC 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 2 cut(s) 383, 476
RsaNI GTAC 2 cut(s) 382, 475
RseI CAYNNNNRTG 2 cut(s) 45, 709
SacI GAGCTC 2 cut(s) 239, 290
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 8 cut(s) 162, 187, 321, 366, 453, 569, 579, 652
Sau96I GGNCC 2 cut(s) 76, 418
SchI GAGTC 3 cut(s) 86, 95, 209
ScrFI CCNGG 1 cut(s) 241
SduI GDGCHC 3 cut(s) 239, 290, 564
SfaNI GCATC 2 cut(s) 136, 666
SfuI TTCGAA 1 cut(s) 555
SinI GGWCC 2 cut(s) 76, 418
SmiMI CAYNNNNRTG 2 cut(s) 45, 709
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SsiI CCGC 1 cut(s) 631
SstI GAGCTC 2 cut(s) 239, 290
StyD4I CCNGG 1 cut(s) 239
StyI CCWWGG 2 cut(s) 99, 414
TaaI ACNGT 1 cut(s) 428
TaqI TCGA 3 cut(s) 165, 555, 750
TaqII GACCGA 2 cut(s) 98, 578
TatI WGTACW 1 cut(s) 474
TfiI GAWTC 4 cut(s) 308, 374, 585, 643
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 1 cut(s) 716
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 1 cut(s) 64
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 4 cut(s) 17, 503, 681, 689
TspGWI ACGGA 2 cut(s) 115, 369
TspRI CASTG 1 cut(s) 716
Tth111I GACNNNGTC 1 cut(s) 85
VpaK11BI GGWCC 2 cut(s) 76, 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.