AT1G66560
WRKY Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0020198
Physical Location & Seq
Forward (+)
24833518 .. 24834801
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66560.1

Sequence Viewer

Length: 750 bp
ATGTTCTCCAACATCGATCAAACAGCTGTGGCAGCACTCCTCCGTGGCCAAGGATGCGCTAACAGTCTCAAGAGGCTCCTCGAAAACCACAAGTTAAGCTCAGATTCAACAGAGCCGCTCATCTACACCATACTCAATTCTTTCTCACTTGCTCTCTCTTTTGTGGACCCGCCTAGTCTTCTACCACACAATGAGTCTTCTCTTCAAAACATGACAAGTCATGTTCTTCAGAGATCATCAAAGAAAAAATATTATGGAGCAGAAGATTTAGAGTATTACAGAGACGAATCCCCGACTCCACGCCCCGATGACGGCTTCACCTGGAGGAAATATGGACAAAAAACTATCAAAACCTCACCGTACCAAAGGTGTTACTATCGGTGTACCTATGCAAAAGACCAAAACTGCAATGCTAGAAAGCGGGTGCAGATGATCCAAGATAACCCTCCAGTGTACAGAACTACTTATTTGGGAAAACATGTGTGTAAAGCTGTTGCAGTTCATGATGATACATATGGTTCTGAAATGATAAAATTCGACCAAGTTGTATCTGAATCGGTTATGCCTCAACTCGCAACAATTGACGAACAAGCAATTACCATGGAGGACGAAGCCATAGACCATATCATGAACCAAGAATGTGATATTAATGATTTTTCTGTGGATGATGACCCATTTTGGGCTAGTCAATTTCCTCCATTTTCATCGGAAGACATTATGTTTTTTGACAATATTGCTAATCTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

249

Amino Acids

28.55

Weight (kDa)

4.91

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 103 - 163 9.3e-23 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020198)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66550 AT1G66550 AT1G66560 AT1G66600 AT1G80590 AT5G01900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 118
AciI CCGC 3 cut(s) 116, 170, 421
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 1 cut(s) 212
AfaI GTAC 3 cut(s) 362, 385, 455
AfiI CCNNNNNNNGG 2 cut(s) 311, 679
AflIII ACRYGT 1 cut(s) 478
AgsI TTSAA 2 cut(s) 108, 206
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 3 cut(s) 26, 99, 491
AluI AGCT 3 cut(s) 26, 99, 491
Alw26I GTCTC 2 cut(s) 71, 276
AlwI GGATC 1 cut(s) 427
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 1 cut(s) 533
AseI ATTAAT 1 cut(s) 648
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 310, 348
AvaII GGWCC 1 cut(s) 166
BalI TGGCCA 1 cut(s) 48
BbsI GAAGAC 3 cut(s) 170, 189, 717
BbvI GCAGC 1 cut(s) 44
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 71, 276
BfaI CTAG 3 cut(s) 174, 414, 684
BisI GCNGC 2 cut(s) 33, 116
BlsI GCNGC 2 cut(s) 34, 117
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 77, 168
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 1 cut(s) 44
BpiI GAAGAC 3 cut(s) 170, 189, 717
BpmI CTGGAG 2 cut(s) 343, 432
BpuEI CTTGAG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 15
BsaJI CCNNGG 3 cut(s) 43, 49, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 679
Bse1I ACTGG 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 322
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 3 cut(s) 43, 49, 600
BseGI GGATG 2 cut(s) 59, 670
BseLI CCNNNNNNNGG 2 cut(s) 311, 679
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 449
BseRI GAGGAG 2 cut(s) 29, 68
BseXI GCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 446
BshFI GGCC 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 311, 679
BsmAI GTCTC 2 cut(s) 71, 276
BsmBI CGTCTC 1 cut(s) 276
BsnI GGCC 1 cut(s) 48
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 3 cut(s) 16, 233, 432
Bsp19I CCATGG 1 cut(s) 600
BspACI CCGC 3 cut(s) 116, 170, 421
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 1 cut(s) 113
BspDI ATCGAT 1 cut(s) 15
BspHI TCATGA 2 cut(s) 502, 627
BspLI GGNNCC 2 cut(s) 77, 168
BspPI GGATC 1 cut(s) 427
BsrBI CCGCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 415
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 3 cut(s) 43, 49, 600
BssMI GATC 3 cut(s) 16, 233, 432
BssT1I CCWWGG 2 cut(s) 49, 600
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 65, 360
Bst6I CTCTTC 1 cut(s) 207
BstAUI TGTACA 1 cut(s) 453
BstDEI CTNAG 1 cut(s) 100
BstDSI CCRYGG 2 cut(s) 43, 600
BstF5I GGATG 2 cut(s) 59, 670
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 3 cut(s) 19, 236, 435
BstMAI GTCTC 2 cut(s) 71, 276
BstMBI GATC 3 cut(s) 16, 233, 432
BstMWI GCNNNNNNNGC 2 cut(s) 32, 54
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 3 cut(s) 170, 189, 717
Bsu15I ATCGAT 1 cut(s) 15
BsuRI GGCC 1 cut(s) 48
BsuTUI ATCGAT 1 cut(s) 15
BtgI CCRYGG 2 cut(s) 43, 600
BtsCI GGATG 2 cut(s) 59, 670
BtsIMutI CAGTG 1 cut(s) 456
CciI TCATGA 2 cut(s) 502, 627
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 166
ClaI ATCGAT 1 cut(s) 15
Csp6I GTAC 3 cut(s) 361, 384, 454
CviAII CATG 6 cut(s) 211, 221, 479, 503, 601, 628
CviJI RGCY 9 cut(s) 26, 48, 76, 99, 115, 315, 491, 614, 683
CviKI_1 RGCY 9 cut(s) 26, 48, 76, 99, 115, 315, 491, 614, 683
CviQI GTAC 3 cut(s) 361, 384, 454
DdeI CTNAG 1 cut(s) 100
DpnI GATC 3 cut(s) 18, 235, 434
DpnII GATC 3 cut(s) 16, 233, 432
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 49, 600
Eco47I GGWCC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 2 cut(s) 49, 600
ErhI CCWWGG 2 cut(s) 49, 600
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 6 cut(s) 214, 224, 482, 506, 604, 631
FatI CATG 6 cut(s) 210, 220, 478, 502, 600, 627
FauI CCCGC 2 cut(s) 177, 414
FauNDI CATATG 1 cut(s) 514
Fnu4HI GCNGC 2 cut(s) 33, 116
FokI GGATG 2 cut(s) 66, 677
Fsp4HI GCNGC 2 cut(s) 33, 116
FspBI CTAG 3 cut(s) 174, 414, 684
GlaI GCGC 1 cut(s) 58
GluI GCNGC 2 cut(s) 33, 116
GsuI CTGGAG 2 cut(s) 343, 432
HaeIII GGCC 1 cut(s) 48
HhaI GCGC 1 cut(s) 59
Hin1II CATG 6 cut(s) 214, 224, 482, 506, 604, 631
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HinfI GANTC 5 cut(s) 104, 194, 287, 295, 554
HphI GGTGA 2 cut(s) 310, 348
Hpy166II GTNNAC 3 cut(s) 166, 384, 454
Hpy188I TCNGA 5 cut(s) 103, 231, 523, 553, 709
Hpy188III TCNNGA 4 cut(s) 70, 503, 628, 743
Hpy8I GTNNAC 3 cut(s) 166, 384, 454
HpyCH4III ACNGT 2 cut(s) 65, 360
HpyCH4V TGCA 4 cut(s) 392, 408, 427, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 54
HpyF3I CTNAG 1 cut(s) 100
Hsp92II CATG 6 cut(s) 214, 224, 482, 506, 604, 631
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 3 cut(s) 16, 233, 432
LmnI GCTCC 2 cut(s) 81, 257
LpnPI CCDG 3 cut(s) 307, 334, 462
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 1 cut(s) 44
MaeI CTAG 3 cut(s) 174, 414, 684
MaeIII GTNAC 1 cut(s) 371
MalI GATC 3 cut(s) 18, 235, 434
MbiI CCGCTC 1 cut(s) 118
MboI GATC 3 cut(s) 16, 233, 432
MboII GAAGA 6 cut(s) 170, 189, 194, 218, 275, 722
MfeI CAATTG 1 cut(s) 579
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 5 cut(s) 136, 533, 579, 594, 689
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 2 cut(s) 203, 289
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 9 cut(s) 50, 66, 89, 318, 364, 456, 576, 598, 705
Mox20I TGGCCA 1 cut(s) 48
MscI TGGCCA 1 cut(s) 48
MseI TTAA 2 cut(s) 95, 648
Msp20I TGGCCA 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 322
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 32, 54
NcoI CCATGG 1 cut(s) 600
NdeI CATATG 1 cut(s) 514
NdeII GATC 3 cut(s) 16, 233, 432
NlaIII CATG 6 cut(s) 214, 224, 482, 506, 604, 631
NlaIV GGNNCC 2 cut(s) 77, 168
NspI RCATGY 1 cut(s) 482
PagI TCATGA 2 cut(s) 502, 627
PciI ACATGT 1 cut(s) 478
PfeI GAWTC 3 cut(s) 104, 287, 554
PkrI GCNGC 2 cut(s) 34, 117
PleI GAGTC 2 cut(s) 202, 289
PpsI GAGTC 2 cut(s) 202, 289
PscI ACATGT 1 cut(s) 478
PshBI ATTAAT 1 cut(s) 648
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 2 cut(s) 77, 168
PspPI GGNCC 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 3 cut(s) 362, 385, 455
RsaNI GTAC 3 cut(s) 361, 384, 454
SaqAI TTAA 2 cut(s) 95, 648
SatI GCNGC 2 cut(s) 33, 116
Sau3AI GATC 3 cut(s) 16, 233, 432
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 2 cut(s) 203, 289
ScrFI CCNGG 1 cut(s) 322
SetI ASST 7 cut(s) 28, 101, 323, 356, 371, 389, 493
SfaNI GCATC 1 cut(s) 44
SinI GGWCC 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 5 cut(s) 136, 533, 579, 594, 689
SsiI CCGC 3 cut(s) 116, 170, 421
SspI AATATT 2 cut(s) 251, 733
SspMI CTAG 3 cut(s) 174, 414, 684
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 2 cut(s) 49, 600
TaaI ACNGT 2 cut(s) 65, 360
TaqI TCGA 3 cut(s) 15, 81, 537
TasI AATT 5 cut(s) 136, 533, 579, 594, 689
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 3 cut(s) 104, 287, 554
Tru1I TTAA 2 cut(s) 95, 648
Tru9I TTAA 2 cut(s) 95, 648
TscAI CASTG 1 cut(s) 456
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 3 cut(s) 491, 644, 693
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 1 cut(s) 456
VpaK11BI GGWCC 1 cut(s) 166
VspI ATTAAT 1 cut(s) 648
XapI RAATTY 1 cut(s) 533
XceI RCATGY 1 cut(s) 482
XspI CTAG 3 cut(s) 174, 414, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.