AT1G66600
WRKY Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0020198
Physical Location & Seq
Forward (+)
24848320 .. 24849543
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66600.1

Sequence Viewer

Length: 726 bp
ATGTTTTCAAACATCGATCACAAGGCTGTGGCAGCACTCCTTCATGGCCAGGGATGCGCTAACATCCTCAAGACGGTCCTCGACAACTGTAAAGTTAGCTCTGTTTCAACAGAACCTCTCATTAACACCATTCTTGATTCCTTTTCACTAGCTCTATCTTCTGTGAATTCTCCTAATCGTCAACCACACCATGAGTCTTCTTCTCGAGACATGGCAGGTCTTGTGCCTCAAAGATCATCAAAGAAGAAGATTTGCGGAGTAAAAGGTTTAGAGATTTACAGAGATGATTCCCCCAATCCCCGCCTTGATGACGGCTTCACCTGGAGGAAATATGGACAAAAAACCATCAAAACCTCTTTGTACCAAAGGTGTTACTATCGTTGTGCCTATGCAAAAGACCAAAACTGCTATGCTACAAAGCGGGTGCAGATGATCCAAGACAGTCCTCCAGTTTACAGAACCACTTATTTGGGACAACATACATGTAAAGCCTTTGGGGTGCATGATAATACGTATGGCTCTGAAATGATAAACTTCGACCAAGTTGTATCTGAATCGGTTATGCGTCAACTTGCAACAATTGGTGAACAAGCTGTTCTCATGGAGGACGAAGCTAACCATATCATGAACCAAGAATATGATATTAATGATTATTTGGTGGATGATGAAGTCTTTTGGGGGAATGAATTTCCTCTATTTTCATCGGAAGACCTGATGTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

241

Amino Acids

27.38

Weight (kDa)

5.63

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 103 - 163 9.4e-22 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020198)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66550 AT1G66550 AT1G66560 AT1G66600 AT1G80590 AT5G01900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 206
AciI CCGC 3 cut(s) 255, 301, 421
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 1 cut(s) 46
AcsI RAATTY 2 cut(s) 166, 686
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 73
AflIII ACRYGT 1 cut(s) 482
AgsI TTSAA 2 cut(s) 9, 108
AjnI CCWGG 2 cut(s) 48, 320
AluBI AGCT 4 cut(s) 99, 152, 593, 614
AluI AGCT 4 cut(s) 99, 152, 593, 614
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 427
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 2 cut(s) 166, 686
AseI ATTAAT 1 cut(s) 645
AspLEI GCGC 1 cut(s) 59
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 310, 596
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 1 cut(s) 76
BalI TGGCCA 1 cut(s) 48
BbsI GAAGAC 2 cut(s) 189, 714
BbvI GCAGC 1 cut(s) 44
BccI CCATC 1 cut(s) 353
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 2 cut(s) 50, 322
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 2 cut(s) 50, 322
Bme18I GGWCC 1 cut(s) 76
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 1 cut(s) 76
BmrFI CCNGG 2 cut(s) 50, 322
BmsI GCATC 1 cut(s) 44
BpiI GAAGAC 2 cut(s) 189, 714
BpmI CTGGAG 2 cut(s) 343, 432
BpuEI CTTGAG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 15
BsaAI YACGTR 1 cut(s) 513
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 1 cut(s) 449
BseBI CCWGG 2 cut(s) 50, 322
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 3 cut(s) 59, 63, 667
BseLI CCNNNNNNNGG 1 cut(s) 73
BseNI ACTGG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 446
BshFI GGCC 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 15
BsiHKCI CYCGRG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 486
BsnI GGCC 1 cut(s) 48
BsoBI CYCGRG 1 cut(s) 204
Bsp143I GATC 3 cut(s) 16, 233, 432
BspACI CCGC 3 cut(s) 255, 301, 421
BspANI GGCC 1 cut(s) 48
BspDI ATCGAT 1 cut(s) 15
BspHI TCATGA 1 cut(s) 624
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 427
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 3 cut(s) 16, 233, 432
Bst2UI CCWGG 2 cut(s) 50, 322
Bst4CI ACNGT 3 cut(s) 76, 89, 443
BstBAI YACGTR 1 cut(s) 513
BstF5I GGATG 3 cut(s) 59, 63, 667
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 3 cut(s) 19, 236, 435
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 3 cut(s) 16, 233, 432
BstMWI GCNNNNNNNGC 2 cut(s) 32, 54
BstNI CCWGG 2 cut(s) 50, 322
BstNSI RCATGY 1 cut(s) 486
BstSCI CCNGG 2 cut(s) 48, 320
BstSNI TACGTA 1 cut(s) 513
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 2 cut(s) 189, 714
BstXI CCANNNNNNTGG 1 cut(s) 469
Bsu15I ATCGAT 1 cut(s) 15
BsuRI GGCC 1 cut(s) 48
BsuTUI ATCGAT 1 cut(s) 15
BtsCI GGATG 3 cut(s) 59, 63, 667
BveI ACCTGC 1 cut(s) 206
CciI TCATGA 1 cut(s) 624
CfoI GCGC 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 76
ClaI ATCGAT 1 cut(s) 15
CseI GACGC 1 cut(s) 554
Csp6I GTAC 1 cut(s) 361
CviAII CATG 7 cut(s) 44, 191, 211, 483, 503, 601, 625
CviJI RGCY 9 cut(s) 26, 48, 99, 152, 315, 491, 519, 593, 614
CviKI_1 RGCY 9 cut(s) 26, 48, 99, 152, 315, 491, 519, 593, 614
CviQI GTAC 1 cut(s) 361
DpnI GATC 3 cut(s) 18, 235, 434
DpnII GATC 3 cut(s) 16, 233, 432
EaeI YGGCCR 1 cut(s) 46
Eco105I TACGTA 1 cut(s) 513
Eco47I GGWCC 1 cut(s) 76
Eco88I CYCGRG 1 cut(s) 204
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 2 cut(s) 48, 320
FaeI CATG 7 cut(s) 47, 194, 214, 486, 506, 604, 628
FaqI GGGAC 1 cut(s) 486
FatI CATG 7 cut(s) 43, 190, 210, 482, 502, 600, 624
FauI CCCGC 2 cut(s) 308, 414
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 3 cut(s) 50, 66, 674
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 1 cut(s) 149
GlaI GCGC 1 cut(s) 58
GluI GCNGC 1 cut(s) 33
GsuI CTGGAG 2 cut(s) 343, 432
HaeIII GGCC 1 cut(s) 48
HgaI GACGC 1 cut(s) 554
HhaI GCGC 1 cut(s) 59
Hin1II CATG 7 cut(s) 47, 194, 214, 486, 506, 604, 628
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 2 cut(s) 182, 569
HindII GTYRAC 2 cut(s) 182, 569
HinfI GANTC 4 cut(s) 137, 194, 287, 554
HphI GGTGA 2 cut(s) 310, 596
Hpy166II GTNNAC 4 cut(s) 182, 454, 569, 587
Hpy188I TCNGA 3 cut(s) 523, 553, 706
Hpy188III TCNNGA 5 cut(s) 70, 134, 204, 206, 625
Hpy8I GTNNAC 4 cut(s) 182, 454, 569, 587
HpyAV CCTTC 1 cut(s) 50
HpyCH4III ACNGT 3 cut(s) 76, 89, 443
HpyCH4IV ACGT 1 cut(s) 512
HpyCH4V TGCA 4 cut(s) 392, 427, 502, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 54
HpySE526I ACGT 1 cut(s) 512
Hsp92II CATG 7 cut(s) 47, 194, 214, 486, 506, 604, 628
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 3 cut(s) 16, 233, 432
LpnPI CCDG 6 cut(s) 35, 62, 201, 307, 334, 462
Lsp1109I GCAGC 1 cut(s) 44
LweI GCATC 1 cut(s) 44
MaeI CTAG 1 cut(s) 149
MaeII ACGT 1 cut(s) 512
MaeIII GTNAC 1 cut(s) 371
MalI GATC 3 cut(s) 18, 235, 434
MboI GATC 3 cut(s) 16, 233, 432
MboII GAAGA 6 cut(s) 150, 189, 192, 256, 259, 719
MfeI CAATTG 1 cut(s) 579
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 3 cut(s) 166, 579, 686
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 9 cut(s) 77, 89, 126, 237, 318, 364, 456, 598, 702
Mox20I TGGCCA 1 cut(s) 48
MscI TGGCCA 1 cut(s) 48
MseI TTAA 2 cut(s) 123, 645
Msp20I TGGCCA 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 50, 322
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 2 cut(s) 50, 322
MwoI GCNNNNNNNGC 2 cut(s) 32, 54
NdeII GATC 3 cut(s) 16, 233, 432
NlaIII CATG 7 cut(s) 47, 194, 214, 486, 506, 604, 628
NspI RCATGY 1 cut(s) 486
PaeR7I CTCGAG 1 cut(s) 204
PagI TCATGA 1 cut(s) 624
PciI ACATGT 1 cut(s) 482
PfeI GAWTC 3 cut(s) 137, 287, 554
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
Ppu21I YACGTR 1 cut(s) 513
PscI ACATGT 1 cut(s) 482
PshBI ATTAAT 1 cut(s) 645
Psp6I CCWGG 2 cut(s) 48, 320
PspGI CCWGG 2 cut(s) 48, 320
PspPI GGNCC 1 cut(s) 76
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SaqAI TTAA 2 cut(s) 123, 645
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 3 cut(s) 16, 233, 432
Sau96I GGNCC 1 cut(s) 76
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 2 cut(s) 50, 322
SfaNI GCATC 1 cut(s) 44
Sfr274I CTCGAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 76
SlaI CTCGAG 1 cut(s) 204
SmlI CTYRAG 2 cut(s) 68, 204
SmoI CTYRAG 2 cut(s) 68, 204
SnaBI TACGTA 1 cut(s) 513
Sse9I AATT 3 cut(s) 166, 579, 686
SsiI CCGC 3 cut(s) 255, 301, 421
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 2 cut(s) 48, 320
TaaI ACNGT 3 cut(s) 76, 89, 443
TaiI ACGT 1 cut(s) 515
TaqI TCGA 4 cut(s) 15, 81, 205, 537
TasI AATT 3 cut(s) 166, 579, 686
TfiI GAWTC 3 cut(s) 137, 287, 554
Tru1I TTAA 2 cut(s) 123, 645
Tru9I TTAA 2 cut(s) 123, 645
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 5 cut(s) 32, 641, 681, 690, 699
VpaK11BI GGWCC 1 cut(s) 76
VspI ATTAAT 1 cut(s) 645
XapI RAATTY 2 cut(s) 166, 686
XceI RCATGY 1 cut(s) 486
XhoI CTCGAG 1 cut(s) 204
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.