AT1G67750

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013798
Physical Location & Seq
Forward (+)
25401514 .. 25403595
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67750.1

Sequence Viewer

Length: 1227 bp
ATGAGAATGACACTTGTTCACTTGTCTCTCTCCCTCTTCTCATGTCTCCTCCTTGTCCTTTCTCCGACCTTCATTGCCTCCACTCCCGTCTCCGAACCCGAACTCGTAGTTCAAGAAGTTAACGAAAAGATTAATGCGTCTAGGAGGAATCTAGGCGTGCTCTCATGTGGGACCGGAAATCCAATTGACGACTGTTGGAGATGCGACCCGAAATGGGAGAAAAACCGACAACGGTTAGCCGATTGCGCGATCGGGTTTGGCAAACACGCAATCGGTGGTCGTGACGGTAAAATCTACGTGGTGACTGACTCGAGTGACAAAGACGTGGTTAACCCTAAACCCGGAACCCTTAGACACGCGGTGATCCAAGACGAGCCACTATGGATCATCTTCGCGCGTGACATGGTCATAAAACTAAAAGAAGAGCTGATTATGAACTCTTTCAAGACTATAGACGGCCGTGGAGCGAGCGTCCACATTGCTGGTGGCGCGTGTATCACCGTCCAGTACGTGACCAACATCATCATCCACGGTGTTAACATCCATGACTGTAAAAGAAAGGGGAATGCTTACGTTAGAGACTCTCCGTCGCATTATGGGTGGAGGACAGCGTCTGACGGTGACGCCGTCTCGATTTTTGGTGGCTCCCACGTGTGGGTAGACCATTGCTCGTTGTCCAACTGCGCTGACGGTCTGATAGACGCCATTCATGGATCAACGGCCATTACTATCTCTAATAACTATTTGAGTCACCACAATAAAGTCATGCTTTTGGGACACAGTGATTCGTACACGAGAGACAAGAACATGCAAGTCACCATTGCCTTTAATCACTTTGGAGAAGGTCTTGTTCAGAGAATGCCAAGATGTAGACATGGATATTTTCATGTGGTGAATAATGATTATACACATTGGCAAATGTATGCAATTGGTGGAAGTGCAGCTCCAACGATTAACAGTCAAGGCAATAGGTTTCTTGCTCCAAACGACCATGTCTTTAAAGAGGTGACTAAATACGAAGATGCACCACGAAGCAAATGGAAGAAATGGAATTGGAGATCGGAAGGTGATTTGTTCCTAAACGGTGCGTTTTTTACGCCTTCGGGTGGAGGAGCCTCTTCAAGCTATGCTAAGGCTTCGAGTTTGTCGGCTAGACCGTCCTCATTGGTGGCTTCAGTCACGTCCAATGCTGGTGCACTCTTTTGTAGAAAAGGATCACGATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

408

Amino Acids

44.89

Weight (kDa)

8.84

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 141 - 346 4.4e-21 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013798)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67750
fragaria_vesca FvH4_4g25110
malus_domestica MD13G1106200.v1.1 MD16G1106400.v1.1
prunus_persica Prupe.1G239900_v2.0.a1 Prupe.1G239900_v2.0.a1
pyrus_communis pycom13g09220 pycom16g09060
rosa_chinensis RchiOBHm_Chr4g0432291
rosa_laevigata RLG00000006855
rosa_multiflora Rmu_sc0001084.1_g000044
rosa_roxburghii Rroxscaffold_5G00373750
rosa_rugosa Rorug04G0256800
rosa_samantha Rh4BG320700 Rh4CG335900 Rh4DG316200
rosa_wichuraiana Rw4G027220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 660, 871
AccII CGCG 5 cut(s) 248, 359, 395, 397, 491
AciI CCGC 1 cut(s) 359
AclWI GGATC 4 cut(s) 358, 392, 721, 1222
AcoI YGGCCR 2 cut(s) 457, 720
AcuI CTGAAG 1 cut(s) 1158
AcvI CACGTG 1 cut(s) 652
AcyI GRCGYC 2 cut(s) 624, 702
AdeI CACNNNGTG 1 cut(s) 361
AfaI GTAC 2 cut(s) 509, 791
AfiI CCNNNNNNNGG 5 cut(s) 214, 341, 654, 655, 1106
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 3 cut(s) 113, 445, 1122
AhdI GACNNNNNGTC 1 cut(s) 586
AjiI CACGTC 2 cut(s) 325, 1182
AluBI AGCT 3 cut(s) 427, 944, 1125
AluI AGCT 3 cut(s) 427, 944, 1125
Alw21I GWGCWC 2 cut(s) 162, 1198
Alw26I GTCTC 6 cut(s) 30, 50, 94, 573, 634, 792
Alw44I GTGCAC 1 cut(s) 1194
AlwI GGATC 4 cut(s) 358, 392, 721, 1222
AlwNI CAGNNNCTG 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 310
AoxI GGCC 2 cut(s) 457, 720
ApaLI GTGCAC 1 cut(s) 1194
ApeKI GCWGC 1 cut(s) 941
AseI ATTAAT 1 cut(s) 132
Asp700I GAANNNNTTC 1 cut(s) 440
AspLEI GCGC 4 cut(s) 248, 397, 491, 686
AspS9I GGNCC 1 cut(s) 171
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 9 cut(s) 313, 373, 490, 632, 743, 808, 904, 1018, 1079
AvaI CYCGRG 1 cut(s) 310
AvaII GGWCC 1 cut(s) 171
BaeGI GKGCMC 1 cut(s) 1198
BauI CACGAG 1 cut(s) 793
BbrPI CACGTG 1 cut(s) 652
Bbv12I GWGCWC 2 cut(s) 162, 1198
BbvI GCAGC 1 cut(s) 953
BceAI ACGGC 4 cut(s) 444, 472, 611, 735
BcnI CCSGG 1 cut(s) 342
BcoDI GTCTC 6 cut(s) 30, 50, 94, 573, 634, 792
BfaI CTAG 3 cut(s) 141, 152, 1152
BfmI CTRYAG 1 cut(s) 450
BisI GCNGC 1 cut(s) 942
BlsI GCNGC 1 cut(s) 943
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 1 cut(s) 171
BmeRI GACNNNNNGTC 1 cut(s) 586
BmeT110I CYCGRG 1 cut(s) 310
BmgBI CACGTC 2 cut(s) 325, 1182
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 4 cut(s) 172, 346, 646, 1114
BmrFI CCNGG 1 cut(s) 342
BmsI GCATC 2 cut(s) 191, 1012
Bpu10I CCTNAGC 1 cut(s) 1131
BpuMI CCSGG 1 cut(s) 342
BsaAI YACGTR 3 cut(s) 298, 511, 652
BsaHI GRCGYC 2 cut(s) 624, 702
BsaJI CCNNGG 2 cut(s) 460, 529
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 341, 654, 655, 1106
Bse1I ACTGG 1 cut(s) 505
Bse3DI GCAATG 4 cut(s) 72, 477, 664, 819
BseDI CCNNGG 2 cut(s) 460, 529
BseGI GGATG 2 cut(s) 525, 540
BseLI CCNNNNNNNGG 5 cut(s) 214, 341, 654, 655, 1106
BseMI GCAATG 4 cut(s) 72, 477, 664, 819
BseNI ACTGG 1 cut(s) 505
BseRI GAGGAG 2 cut(s) 38, 1125
BseSI GKGCMC 1 cut(s) 1198
BseX3I CGGCCG 1 cut(s) 457
BseXI GCAGC 1 cut(s) 953
BsgI GTGCAG 1 cut(s) 960
Bsh1236I CGCG 5 cut(s) 248, 359, 395, 397, 491
Bsh1285I CGRYCG 2 cut(s) 252, 460
BshFI GGCC 2 cut(s) 459, 722
BsiEI CGRYCG 2 cut(s) 252, 460
BsiHKAI GWGCWC 2 cut(s) 162, 1198
BsiHKCI CYCGRG 1 cut(s) 310
BsiSI CCGG 2 cut(s) 174, 342
BslFI GGGAC 2 cut(s) 184, 789
BslI CCNNNNNNNGG 5 cut(s) 214, 341, 654, 655, 1106
BsmAI GTCTC 6 cut(s) 30, 50, 94, 573, 634, 792
BsmBI CGTCTC 2 cut(s) 94, 634
BsmFI GGGAC 2 cut(s) 184, 789
BsmI GAATGC 2 cut(s) 571, 864
BsnI GGCC 2 cut(s) 459, 722
BsoBI CYCGRG 1 cut(s) 310
Bsp1286I GDGCHC 2 cut(s) 162, 1198
Bsp143I GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
BspACI CCGC 1 cut(s) 359
BspANI GGCC 2 cut(s) 459, 722
BspFNI CGCG 5 cut(s) 248, 359, 395, 397, 491
BspLI GGNNCC 4 cut(s) 172, 346, 646, 1114
BspPI GGATC 4 cut(s) 358, 392, 721, 1222
BspQI GCTCTTC 1 cut(s) 417
BsrDI GCAATG 4 cut(s) 72, 477, 664, 819
BsrI ACTGG 1 cut(s) 505
BssECI CCNNGG 2 cut(s) 460, 529
BssMI GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
BssNI GRCGYC 2 cut(s) 624, 702
BssSI CACGAG 1 cut(s) 793
Bst2BI CACGAG 1 cut(s) 793
Bst6I CTCTTC 3 cut(s) 41, 417, 1123
BstACI GRCGYC 2 cut(s) 624, 702
BstBAI YACGTR 3 cut(s) 298, 511, 652
BstC8I GCNNGC 2 cut(s) 158, 469
BstDEI CTNAG 2 cut(s) 350, 1131
BstDSI CCRYGG 2 cut(s) 460, 529
BstF5I GGATG 2 cut(s) 525, 540
BstFNI CGCG 5 cut(s) 248, 359, 395, 397, 491
BstHHI GCGC 4 cut(s) 248, 397, 491, 686
BstKTI GATC 6 cut(s) 252, 366, 387, 716, 1061, 1217
BstMAI GTCTC 6 cut(s) 30, 50, 94, 573, 634, 792
BstMBI GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
BstMCI CGRYCG 2 cut(s) 252, 460
BstMWI GCNNNNNNNGC 2 cut(s) 245, 488
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 1198
BstUI CGCG 5 cut(s) 248, 359, 395, 397, 491
BstV1I GCAGC 1 cut(s) 953
BstXI CCANNNNNNTGG 1 cut(s) 482
BstZI CGGCCG 1 cut(s) 457
BsuRI GGCC 2 cut(s) 459, 722
BtgI CCRYGG 2 cut(s) 460, 529
BtrI CACGTC 2 cut(s) 325, 1182
BtsCI GGATG 2 cut(s) 525, 540
BtsIMutI CAGTG 1 cut(s) 787
Cac8I GCNNGC 2 cut(s) 158, 469
CaiI CAGNNNCTG 1 cut(s) 614
CfoI GCGC 4 cut(s) 248, 397, 491, 686
Cfr13I GGNCC 1 cut(s) 171
CseI GACGC 5 cut(s) 126, 460, 600, 632, 710
Csp6I GTAC 2 cut(s) 508, 790
CviQI GTAC 2 cut(s) 508, 790
DdeI CTNAG 2 cut(s) 350, 1131
DpnI GATC 6 cut(s) 251, 365, 386, 715, 1060, 1216
DpnII GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
DraI TTTAAA 1 cut(s) 1000
DraIII CACNNNGTG 1 cut(s) 361
DriI GACNNNNNGTC 1 cut(s) 586
EaeI YGGCCR 2 cut(s) 457, 720
EagI CGGCCG 1 cut(s) 457
Eam1104I CTCTTC 3 cut(s) 41, 417, 1123
Eam1105I GACNNNNNGTC 1 cut(s) 586
EarI CTCTTC 3 cut(s) 41, 417, 1123
EclXI CGGCCG 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 171
Eco52I CGGCCG 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 1158
Eco72I CACGTG 1 cut(s) 652
Eco88I CYCGRG 1 cut(s) 310
Esp3I CGTCTC 2 cut(s) 94, 634
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FaqI GGGAC 2 cut(s) 184, 789
FblI GTMKAC 2 cut(s) 660, 871
Fnu4HI GCNGC 1 cut(s) 942
FokI GGATG 2 cut(s) 512, 527
Fsp4HI GCNGC 1 cut(s) 942
FspBI CTAG 3 cut(s) 141, 152, 1152
GlaI GCGC 4 cut(s) 247, 396, 490, 685
GluI GCNGC 1 cut(s) 942
HaeIII GGCC 2 cut(s) 459, 722
HapII CCGG 2 cut(s) 174, 342
HgaI GACGC 5 cut(s) 126, 460, 600, 632, 710
HhaI GCGC 4 cut(s) 248, 397, 491, 686
Hin1I GRCGYC 2 cut(s) 624, 702
Hin6I GCGC 4 cut(s) 246, 395, 489, 684
HinP1I GCGC 4 cut(s) 246, 395, 489, 684
HincII GTYRAC 3 cut(s) 121, 331, 538
HindII GTYRAC 3 cut(s) 121, 331, 538
HinfI GANTC 5 cut(s) 148, 308, 581, 748, 785
HpaI GTTAAC 3 cut(s) 121, 331, 538
HpaII CCGG 2 cut(s) 174, 342
HphI GGTGA 9 cut(s) 313, 373, 490, 632, 743, 808, 904, 1018, 1079
Hpy166II GTNNAC 9 cut(s) 19, 121, 331, 475, 538, 661, 792, 872, 1196
Hpy188I TCNGA 6 cut(s) 66, 94, 616, 696, 855, 1063
Hpy188III TCNNGA 5 cut(s) 113, 281, 445, 631, 1218
Hpy8I GTNNAC 9 cut(s) 19, 121, 331, 475, 538, 661, 792, 872, 1196
Hpy99I CGWCG 1 cut(s) 592
HpyAV CCTTC 4 cut(s) 79, 836, 1058, 1110
HpyCH4IV ACGT 6 cut(s) 297, 324, 510, 573, 651, 1181
HpyCH4V TGCA 5 cut(s) 811, 926, 941, 1025, 1196
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 488
HpyF3I CTNAG 2 cut(s) 350, 1131
HpySE526I ACGT 6 cut(s) 297, 324, 510, 573, 651, 1181
Hsp92I GRCGYC 2 cut(s) 624, 702
HspAI GCGC 4 cut(s) 246, 395, 489, 684
KspAI GTTAAC 3 cut(s) 121, 331, 538
Kzo9I GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 5 cut(s) 464, 650, 949, 985, 1112
LpnPI CCDG 5 cut(s) 187, 355, 468, 518, 1176
Lsp1109I GCAGC 1 cut(s) 953
LweI GCATC 2 cut(s) 191, 1012
MaeI CTAG 3 cut(s) 141, 152, 1152
MaeII ACGT 6 cut(s) 297, 324, 510, 573, 651, 1181
MalI GATC 6 cut(s) 251, 365, 386, 715, 1060, 1216
MboI GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
MboII GAAGA 6 cut(s) 28, 382, 434, 1031, 1054, 1110
MfeI CAATTG 2 cut(s) 183, 927
MhlI GDGCHC 2 cut(s) 162, 1198
MluCI AATT 3 cut(s) 183, 927, 1051
MlyI GAGTC 3 cut(s) 302, 575, 757
MmeI TCCRAC 4 cut(s) 89, 176, 702, 971
MnlI CCTC 9 cut(s) 44, 59, 88, 138, 597, 997, 1103, 1126, 1171
MroXI GAANNNNTTC 1 cut(s) 440
MseI TTAA 8 cut(s) 120, 132, 330, 537, 828, 954, 999, 1225
MspI CCGG 2 cut(s) 174, 342
MspR9I CCNGG 1 cut(s) 342
MunI CAATTG 2 cut(s) 183, 927
Mva1269I GAATGC 2 cut(s) 571, 864
MvnI CGCG 5 cut(s) 248, 359, 395, 397, 491
MwoI GCNNNNNNNGC 2 cut(s) 245, 488
NciI CCSGG 1 cut(s) 342
NdeII GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
NlaIV GGNNCC 4 cut(s) 172, 346, 646, 1114
NspI RCATGY 1 cut(s) 811
PaeR7I CTCGAG 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 417
PcsI WCGNNNNNNNCGW 2 cut(s) 624, 1154
PctI GAATGC 2 cut(s) 571, 864
PdmI GAANNNNTTC 1 cut(s) 440
PfeI GAWTC 2 cut(s) 148, 785
PflFI GACNNNGTC 4 cut(s) 404, 610, 626, 992
PkrI GCNGC 1 cut(s) 943
Ple19I CGATCG 1 cut(s) 252
PleI GAGTC 3 cut(s) 302, 575, 756
PmaCI CACGTG 1 cut(s) 652
PmlI CACGTG 1 cut(s) 652
PpsI GAGTC 3 cut(s) 302, 575, 756
Ppu21I YACGTR 3 cut(s) 298, 511, 652
PshBI ATTAAT 1 cut(s) 132
PspCI CACGTG 1 cut(s) 652
PspN4I GGNNCC 4 cut(s) 172, 346, 646, 1114
PspPI GGNCC 1 cut(s) 171
PspXI VCTCGAGB 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 614
PsyI GACNNNGTC 4 cut(s) 404, 610, 626, 992
PvuI CGATCG 1 cut(s) 252
RsaI GTAC 2 cut(s) 509, 791
RsaNI GTAC 2 cut(s) 508, 790
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 8 cut(s) 120, 132, 330, 537, 828, 954, 999, 1225
SatI GCNGC 1 cut(s) 942
Sau3AI GATC 6 cut(s) 249, 363, 384, 713, 1058, 1214
Sau96I GGNCC 1 cut(s) 171
SchI GAGTC 3 cut(s) 302, 575, 757
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 2 cut(s) 162, 1198
SfaNI GCATC 2 cut(s) 191, 1012
SfcI CTRYAG 1 cut(s) 450
Sfr274I CTCGAG 1 cut(s) 310
SinI GGWCC 1 cut(s) 171
SlaI CTCGAG 1 cut(s) 310
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
Sse9I AATT 3 cut(s) 183, 927, 1051
SsiI CCGC 1 cut(s) 359
SspMI CTAG 3 cut(s) 141, 152, 1152
StyD4I CCNGG 1 cut(s) 340
TaiI ACGT 6 cut(s) 300, 327, 513, 576, 654, 1184
TaqI TCGA 3 cut(s) 311, 632, 1139
TasI AATT 3 cut(s) 183, 927, 1051
TfiI GAWTC 2 cut(s) 148, 785
Tru1I TTAA 8 cut(s) 120, 132, 330, 537, 828, 954, 999, 1225
Tru9I TTAA 8 cut(s) 120, 132, 330, 537, 828, 954, 999, 1225
TscAI CASTG 1 cut(s) 787
TseI GCWGC 1 cut(s) 941
TspDTI ATGAA 4 cut(s) 61, 449, 698, 875
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 1 cut(s) 787
Tth111I GACNNNGTC 4 cut(s) 404, 610, 626, 992
VneI GTGCAC 1 cut(s) 1194
VpaK11BI GGWCC 1 cut(s) 171
VspI ATTAAT 1 cut(s) 132
XceI RCATGY 1 cut(s) 811
XcmI CCANNNNNNNNNTGG 2 cut(s) 482, 1035
XhoI CTCGAG 1 cut(s) 310
XmiI GTMKAC 2 cut(s) 660, 871
XmnI GAANNNNTTC 1 cut(s) 440
XspI CTAG 3 cut(s) 141, 152, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.