MD13G1106200.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013798
Physical Location & Seq
Reverse (-)
7620362 .. 7622557
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1106200.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGCAATGCCACTCTCCCTTTTGCTGATCCTCTCTCTCCTCCTAATCCCAACAACCATTTCCTCCTCCCCGGTCAAAGTTCCTGAACTGGTGATGCAACAAGTACAAGAGAGCATCAATGCCTCAAGGAGAAATTTGGGTTATCTTTCATGTGGCACCGGAAACCCCATTGACGACTGCTGGAGGTGTGACCCCAACTGGGAGAAGAACCGCCAGCGGCTAGCCGACTGCGCAATCGGGTTCGGAAAGCACGCCATTGGCGGCAGGGACGGTAAAATCTACGTGGTCACGGACTCAGGCGACCATCCGGTGAACCCTAAACCCGGAACCCTCCGATACGGCGTGATCCAAAACGAACCGCTCTGGATCGTATTCCAGCGTGACATGACGATCAAGCTGAAAGAAGAACTGATGATGAACTCCTTCAAGACCATCGACGGCAGAGGAGCCAGCGTCCACATTGCCGGTGGGCCCTGCATTACCATACAGTACGTGACCAACATTATCATCCACGGATTAAACATTCACGACTGCAAGCAAGGCGGGAACGCCTATGTGAGGGACTCGCCGGAGCATTTTGGGTGGCGGACGCTTTCCGACGGAGACGGGGTTTCTATTTTTGGTGGTAGTCATGTTTGGGTGGATCATAACTCTCTTTCGAACTGCCGTGACGGGCTCATTGATGCCATACACGGTTCCACTTCCATCACCATTTCTAACAATTACATGACGCATCATGACAAGGTCATGCTCTTGGGGCACAGTGATTCCTACACTCAGGACAAGAATATGCAGGTCACCATTGCATTCAACCACTTTGGAGAAGGCCTTGTTCAGAGGATGCCAAGATGTAGACATGGGAACTTTCATGTGGTGAACAATGACTACACCCATTGGGAAATGTATGCTATTGGCGGGAGTGCTTCTCCAACCATCAACAGCCAAGGCAACAGATTTCTAGCACCAAATGATAGATTCAACAAAGAGGTCACCAAGCATGAGGATGCGCCACAGAAGGAGTGGAGCAAATGGAACTGGAGGTCCTCGGGAGATTTGCTGCTAAACGGTGCGTTCTTCACAGCATCCGGTGCAGGAGCATCTTCTAGTTACGCCAAGGCATCGAGCTTGGGTGCGAGACCGTCGTCGCTCGTGAGTTCACTCACTGCCGGAGCTGGTTCGCTCAGGTGCAAGAAGGGCTCGCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

403

Amino Acids

44.13

Weight (kDa)

8.47

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 134 - 335 8.9e-21 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013798)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67750
fragaria_vesca FvH4_4g25110
malus_domestica MD13G1106200.v1.1 MD16G1106400.v1.1
prunus_persica Prupe.1G239900_v2.0.a1 Prupe.1G239900_v2.0.a1
pyrus_communis pycom13g09220 pycom16g09060
rosa_chinensis RchiOBHm_Chr4g0432291
rosa_laevigata RLG00000006855
rosa_multiflora Rmu_sc0001084.1_g000044
rosa_roxburghii Rroxscaffold_5G00373750
rosa_rugosa Rorug04G0256800
rosa_samantha Rh4BG320700 Rh4CG335900 Rh4DG316200
rosa_wichuraiana Rw4G027220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 232
Acc36I ACCTGC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 361
AccI GTMKAC 1 cut(s) 853
AccII CGCG 1 cut(s) 1201
AciI CCGC 7 cut(s) 211, 217, 261, 359, 543, 586, 915
AclWI GGATC 4 cut(s) 22, 340, 374, 651
AcsI RAATTY 1 cut(s) 133
AfaI GTAC 2 cut(s) 105, 491
AfiI CCNNNNNNNGG 3 cut(s) 199, 323, 558
AgsI TTSAA 3 cut(s) 427, 811, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AloI GAACNNNNNNTCC 2 cut(s) 1025, 1057
AluBI AGCT 3 cut(s) 397, 1125, 1172
AluI AGCT 3 cut(s) 397, 1125, 1172
Alw26I GTCTC 2 cut(s) 597, 1129
AlwI GGATC 4 cut(s) 22, 340, 374, 651
Ama87I CYCGRG 1 cut(s) 1045
AoxI GGCC 2 cut(s) 470, 826
ApaI GGGCCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 1057
ApoI RAATTY 1 cut(s) 133
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 2 cut(s) 233, 1009
AspS9I GGNCC 3 cut(s) 470, 471, 1041
AsuC2I CCSGG 2 cut(s) 71, 324
AsuHPI GGTGA 6 cut(s) 103, 322, 700, 790, 886, 982
AsuII TTCGAA 1 cut(s) 659
AsuNHI GCTAGC 1 cut(s) 220
AvaI CYCGRG 1 cut(s) 1045
AvaII GGWCC 1 cut(s) 1041
BaeGI GKGCMC 2 cut(s) 474, 762
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 3 cut(s) 474, 678, 1199
BauI CACGAG 1 cut(s) 1148
BbvI GCAGC 1 cut(s) 1044
BccI CCATC 4 cut(s) 312, 440, 713, 941
BceAI ACGGC 3 cut(s) 355, 454, 651
BcnI CCSGG 2 cut(s) 71, 324
BcoDI GTCTC 2 cut(s) 597, 1129
BfaI CTAG 3 cut(s) 221, 959, 1104
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 3 cut(s) 218, 262, 1058
BlsI GCNGC 3 cut(s) 219, 263, 1059
Bme1390I CCNGG 2 cut(s) 71, 324
Bme18I GGWCC 1 cut(s) 1041
BmeT110I CYCGRG 1 cut(s) 1045
BmgT120I GGNCC 3 cut(s) 470, 471, 1041
BmiI GGNNCC 5 cut(s) 157, 328, 448, 472, 697
BmrFI CCNGG 2 cut(s) 71, 324
BmrI ACTGGG 1 cut(s) 208
BmsI GCATC 9 cut(s) 84, 123, 673, 742, 831, 994, 1091, 1106, 1127
BmtI GCTAGC 1 cut(s) 224
BmuI ACTGGG 1 cut(s) 208
BoxI GACNNNNGTC 1 cut(s) 1141
BplI GAGNNNNNCTC 2 cut(s) 910, 942
BpmI CTGGAG 2 cut(s) 202, 1057
Bpu10I CCTNAGC 1 cut(s) 1181
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 2 cut(s) 71, 324
BsaAI YACGTR 2 cut(s) 283, 493
BsaI GGTCTC 1 cut(s) 1129
BsaJI CCNNGG 5 cut(s) 69, 511, 943, 1044, 1113
BsaWI WCCGGW 3 cut(s) 158, 307, 1085
BsaXI ACNNNNNCTCC 4 cut(s) 438, 468, 594, 624
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 323, 558
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 3 cut(s) 93, 203, 1040
Bse3DI GCAATG 3 cut(s) 12, 459, 801
BseDI CCNNGG 5 cut(s) 69, 511, 943, 1044, 1113
BseGI GGATG 5 cut(s) 304, 507, 846, 1009, 1082
BseLI CCNNNNNNNGG 3 cut(s) 199, 323, 558
BseMI GCAATG 3 cut(s) 12, 459, 801
BseMII CTCAG 3 cut(s) 309, 791, 1195
BseNI ACTGG 3 cut(s) 93, 203, 1040
BseRI GAGGAG 3 cut(s) 29, 55, 459
BseSI GKGCMC 2 cut(s) 474, 762
BseXI GCAGC 1 cut(s) 1044
BsgI GTGCAG 1 cut(s) 1110
Bsh1236I CGCG 1 cut(s) 1201
BshFI GGCC 2 cut(s) 472, 828
BshNI GGYRCC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 1045
BsiSI CCGG 8 cut(s) 71, 159, 308, 324, 465, 569, 1086, 1167
BslFI GGGAC 2 cut(s) 281, 575
BslI CCNNNNNNNGG 3 cut(s) 199, 323, 558
BsmAI GTCTC 2 cut(s) 597, 1129
BsmBI CGTCTC 1 cut(s) 597
BsmFI GGGAC 2 cut(s) 281, 575
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 2 cut(s) 472, 828
Bso31I GGTCTC 1 cut(s) 1129
BsoBI CYCGRG 1 cut(s) 1045
Bsp119I TTCGAA 1 cut(s) 659
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 4 cut(s) 474, 678, 762, 1199
Bsp143I GATC 5 cut(s) 27, 345, 366, 390, 643
BspACI CCGC 7 cut(s) 211, 217, 261, 359, 543, 586, 915
BspANI GGCC 2 cut(s) 472, 828
BspCNI CTCAG 3 cut(s) 308, 790, 1194
BspFNI CGCG 1 cut(s) 1201
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 5 cut(s) 157, 328, 448, 472, 697
BspMI ACCTGC 1 cut(s) 784
BspOI GCTAGC 1 cut(s) 224
BspPI GGATC 4 cut(s) 22, 340, 374, 651
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 1129
BsrBI CCGCTC 1 cut(s) 361
BsrDI GCAATG 3 cut(s) 12, 459, 801
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 3 cut(s) 93, 203, 1040
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 5 cut(s) 69, 511, 943, 1044, 1113
BssMI GATC 5 cut(s) 27, 345, 366, 390, 643
BssSI CACGAG 1 cut(s) 1148
BssT1I CCWWGG 2 cut(s) 943, 1113
Bst2BI CACGAG 1 cut(s) 1148
Bst4CI ACNGT 6 cut(s) 272, 489, 695, 764, 1067, 1140
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstBAI YACGTR 2 cut(s) 283, 493
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 6 cut(s) 215, 222, 252, 451, 536, 1199
BstDEI CTNAG 3 cut(s) 295, 777, 1181
BstDSI CCRYGG 1 cut(s) 511
BstEII GGTNACC 2 cut(s) 796, 988
BstENI CCTNNNNNAGG 1 cut(s) 556
BstF5I GGATG 5 cut(s) 304, 507, 846, 1009, 1082
BstFNI CGCG 1 cut(s) 1201
BstHHI GCGC 2 cut(s) 233, 1009
BstKTI GATC 5 cut(s) 30, 348, 369, 393, 646
BstMAI GTCTC 2 cut(s) 597, 1129
BstMBI GATC 5 cut(s) 27, 345, 366, 390, 643
BstMWI GCNNNNNNNGC 6 cut(s) 230, 540, 757, 1088, 1194, 1203
BstPAI GACNNNNGTC 1 cut(s) 1141
BstPI GGTNACC 2 cut(s) 796, 988
BstSCI CCNGG 2 cut(s) 69, 322
BstSLI GKGCMC 2 cut(s) 474, 762
BstUI CGCG 1 cut(s) 1201
BstV1I GCAGC 1 cut(s) 1044
BsuRI GGCC 2 cut(s) 472, 828
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 5 cut(s) 304, 507, 846, 1009, 1082
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 2 cut(s) 769, 1161
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 6 cut(s) 215, 222, 252, 451, 536, 1199
CciI TCATGA 1 cut(s) 736
CfoI GCGC 2 cut(s) 233, 1009
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 3 cut(s) 470, 471, 1041
CseI GACGC 3 cut(s) 442, 598, 739
Csp6I GTAC 2 cut(s) 104, 490
CviAII CATG 9 cut(s) 150, 385, 632, 727, 737, 748, 857, 869, 998
CviQI GTAC 2 cut(s) 104, 490
DdeI CTNAG 3 cut(s) 295, 777, 1181
DpnI GATC 5 cut(s) 29, 347, 368, 392, 645
DpnII GATC 5 cut(s) 27, 345, 366, 390, 643
EciI GGCGGA 1 cut(s) 601
Eco130I CCWWGG 2 cut(s) 943, 1113
Eco147I AGGCCT 1 cut(s) 828
Eco24I GRGCYC 3 cut(s) 474, 678, 1199
Eco31I GGTCTC 1 cut(s) 1129
Eco47I GGWCC 1 cut(s) 1041
Eco88I CYCGRG 1 cut(s) 1045
Eco91I GGTNACC 2 cut(s) 796, 988
EcoNI CCTNNNNNAGG 1 cut(s) 556
EcoO109I RGGNCCY 2 cut(s) 471, 1041
EcoO65I GGTNACC 2 cut(s) 796, 988
EcoT14I CCWWGG 2 cut(s) 943, 1113
EcoT38I GRGCYC 3 cut(s) 474, 678, 1199
ErhI CCWWGG 2 cut(s) 943, 1113
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 9 cut(s) 153, 388, 635, 730, 740, 751, 860, 872, 1001
FaqI GGGAC 2 cut(s) 281, 575
FatI CATG 9 cut(s) 149, 384, 631, 726, 736, 747, 856, 868, 997
FauI CCCGC 2 cut(s) 536, 908
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 3 cut(s) 218, 262, 1058
FokI GGATG 5 cut(s) 291, 494, 853, 1016, 1069
FriOI GRGCYC 3 cut(s) 474, 678, 1199
Fsp4HI GCNGC 3 cut(s) 218, 262, 1058
FspBI CTAG 3 cut(s) 221, 959, 1104
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 2 cut(s) 232, 1008
GluI GCNGC 3 cut(s) 218, 262, 1058
GsuI CTGGAG 2 cut(s) 202, 1057
HaeIII GGCC 2 cut(s) 472, 828
HapII CCGG 8 cut(s) 71, 159, 308, 324, 465, 569, 1086, 1167
HgaI GACGC 3 cut(s) 442, 598, 739
HhaI GCGC 2 cut(s) 233, 1009
Hin1II CATG 9 cut(s) 153, 388, 635, 730, 740, 751, 860, 872, 1001
Hin6I GCGC 2 cut(s) 231, 1007
HinP1I GCGC 2 cut(s) 231, 1007
HinfI GANTC 4 cut(s) 293, 563, 767, 975
HpaII CCGG 8 cut(s) 71, 159, 308, 324, 465, 569, 1086, 1167
HphI GGTGA 6 cut(s) 103, 322, 700, 790, 886, 982
Hpy166II GTNNAC 5 cut(s) 313, 457, 854, 877, 1157
Hpy188I TCNGA 4 cut(s) 245, 335, 598, 837
Hpy188III TCNNGA 8 cut(s) 83, 364, 427, 527, 737, 779, 1047, 1150
Hpy8I GTNNAC 5 cut(s) 313, 457, 854, 877, 1157
Hpy99I CGWCG 4 cut(s) 440, 602, 1144, 1147
HpyAV CCTTC 4 cut(s) 433, 818, 1009, 1186
HpyCH4III ACNGT 6 cut(s) 272, 489, 695, 764, 1067, 1140
HpyCH4IV ACGT 2 cut(s) 282, 492
HpyCH4V TGCA 7 cut(s) 97, 477, 534, 793, 806, 1091, 1188
HpyF10VI GCNNNNNNNGC 6 cut(s) 230, 540, 757, 1088, 1194, 1203
HpyF3I CTNAG 3 cut(s) 295, 777, 1181
HpySE526I ACGT 2 cut(s) 282, 492
Hsp92II CATG 9 cut(s) 153, 388, 635, 730, 740, 751, 860, 872, 1001
HspAI GCGC 2 cut(s) 231, 1007
Kzo9I GATC 5 cut(s) 27, 345, 366, 390, 643
LmnI GCTCC 5 cut(s) 446, 571, 1023, 1094, 1169
Lsp1109I GCAGC 1 cut(s) 1044
LweI GCATC 9 cut(s) 84, 123, 673, 742, 831, 994, 1091, 1106, 1127
MaeI CTAG 3 cut(s) 221, 959, 1104
MaeII ACGT 2 cut(s) 282, 492
MaeIII GTNAC 8 cut(s) 188, 286, 380, 493, 668, 796, 988, 1106
MalI GATC 5 cut(s) 29, 347, 368, 392, 645
MbiI CCGCTC 1 cut(s) 361
MboI GATC 5 cut(s) 27, 345, 366, 390, 643
MboII GAAGA 4 cut(s) 217, 416, 1066, 1092
MhlI GDGCHC 4 cut(s) 474, 678, 762, 1199
MluCI AATT 2 cut(s) 133, 721
MlyI GAGTC 2 cut(s) 287, 557
MmeI TCCRAC 2 cut(s) 621, 953
MroXI GAANNNNTTC 1 cut(s) 422
MseI TTAA 1 cut(s) 518
MslI CAYNNNNRTG 1 cut(s) 1002
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 8 cut(s) 71, 159, 308, 324, 465, 569, 1086, 1167
MspR9I CCNGG 2 cut(s) 71, 324
Mva1269I GAATGC 1 cut(s) 806
MvnI CGCG 1 cut(s) 1201
MwoI GCNNNNNNNGC 6 cut(s) 230, 540, 757, 1088, 1194, 1203
NciI CCSGG 2 cut(s) 71, 324
NdeII GATC 5 cut(s) 27, 345, 366, 390, 643
NheI GCTAGC 1 cut(s) 220
NlaIII CATG 9 cut(s) 153, 388, 635, 730, 740, 751, 860, 872, 1001
NlaIV GGNNCC 5 cut(s) 157, 328, 448, 472, 697
NmuCI GTSAC 7 cut(s) 188, 286, 380, 493, 668, 796, 988
NsbI TGCGCA 1 cut(s) 232
NspV TTCGAA 1 cut(s) 659
PagI TCATGA 1 cut(s) 736
PceI AGGCCT 1 cut(s) 828
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 2 cut(s) 767, 975
PflFI GACNNNGTC 1 cut(s) 743
PkrI GCNGC 3 cut(s) 219, 263, 1059
PleI GAGTC 2 cut(s) 287, 557
PpsI GAGTC 2 cut(s) 287, 557
Ppu21I YACGTR 2 cut(s) 283, 493
PpuMI RGGWCCY 1 cut(s) 1041
PshAI GACNNNNGTC 1 cut(s) 1141
Psp5II RGGWCCY 1 cut(s) 1041
PspEI GGTNACC 2 cut(s) 796, 988
PspN4I GGNNCC 5 cut(s) 157, 328, 448, 472, 697
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 3 cut(s) 470, 471, 1041
PspPPI RGGWCCY 1 cut(s) 1041
PsrI GAACNNNNNNTAC 2 cut(s) 869, 901
PsyI GACNNNGTC 1 cut(s) 743
RsaI GTAC 2 cut(s) 105, 491
RsaNI GTAC 2 cut(s) 104, 490
RseI CAYNNNNRTG 1 cut(s) 1002
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 3 cut(s) 218, 262, 1058
Sau3AI GATC 5 cut(s) 27, 345, 366, 390, 643
Sau96I GGNCC 3 cut(s) 470, 471, 1041
SchI GAGTC 2 cut(s) 287, 557
ScrFI CCNGG 2 cut(s) 71, 324
SduI GDGCHC 4 cut(s) 474, 678, 762, 1199
SfaNI GCATC 9 cut(s) 84, 123, 673, 742, 831, 994, 1091, 1106, 1127
SfuI TTCGAA 1 cut(s) 659
SinI GGWCC 1 cut(s) 1041
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 2 cut(s) 133, 721
SseBI AGGCCT 1 cut(s) 828
SsiI CCGC 7 cut(s) 211, 217, 261, 359, 543, 586, 915
SspMI CTAG 3 cut(s) 221, 959, 1104
StuI AGGCCT 1 cut(s) 828
StyD4I CCNGG 2 cut(s) 69, 322
StyI CCWWGG 2 cut(s) 943, 1113
TaaI ACNGT 6 cut(s) 272, 489, 695, 764, 1067, 1140
TaiI ACGT 2 cut(s) 285, 495
TaqI TCGA 3 cut(s) 435, 659, 1121
TasI AATT 2 cut(s) 133, 721
TatI WGTACW 1 cut(s) 103
TauI GCSGC 2 cut(s) 220, 264
TfiI GAWTC 2 cut(s) 767, 975
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TscAI CASTG 2 cut(s) 769, 1168
TseFI GTSAC 7 cut(s) 188, 286, 380, 493, 668, 796, 988
TseI GCWGC 1 cut(s) 1057
Tsp45I GTSAC 7 cut(s) 188, 286, 380, 493, 668, 796, 988
TspDTI ATGAA 3 cut(s) 138, 431, 857
TspGWI ACGGA 3 cut(s) 305, 528, 615
TspRI CASTG 2 cut(s) 769, 1168
Tth111I GACNNNGTC 1 cut(s) 743
VpaK11BI GGWCC 1 cut(s) 1041
XagI CCTNNNNNAGG 1 cut(s) 556
XapI RAATTY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 2 cut(s) 464, 1017
XmiI GTMKAC 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 3 cut(s) 221, 959, 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.