pycom13g09220

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013798
Physical Location & Seq
Reverse (-)
6170406 .. 6172381
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09220.1

Sequence Viewer

Length: 1209 bp
ATGGCAATGCCACTCTCCCTTTTGCTGATCCTCTCTCTCCTCCTAATCCCAACAACCATTTCCTCCTCCCCAGTCAAAGATCCTGAACTGGTGATGCAACAAGTACAAGAGAGCATCAATGCCTCAAGGAGAAATTTGGGTTATCTTTCCTGTGGCACCGGAAACCCCATTGACGACTGCTGGAGGTGTGACCCCAACTGGGAGAAGAACCGCCAGCGGCTAGCCGACTGCGCAATCGGGTTCGGAAAGCAGGCCATTGGCGGCAGGGACGGTAAAATCTACGTGGTCACGGACTCAGGCGACCATCCGGTGAACCCTAAACCCGGAACCCTCCGATACGGCGTGATCCAAAACGAACCGCTCTGGATCGTATTCCAGCGTGACATGACGATCAAGCTGAAAGAAGAACTGATGATGAACTCCTTCAAGACCATCGACGGCAGAGGAGCCAGCGTCCACATTGCCGGTGGGCCCTGCATTACCATACAGTACGTGACCAACATTATCATCCACGGATTAAACATTCACGACTGCAAGCAAGGAGGGAACGCCTACGTGAGGGACTCGCCGGAGCATTTTGGGTGGCGGACGCTTTCCGACGGAGACGGGGTTTCCATTTTTGGTGGTAGTCATGTTTGGGTGGATCATAACTCTCTTTCGAACTGCCGTGACGGGCTCATCGATGCCATCCATGGTTCCACTTCCATCACCATTTCTAACAACTACATGACGCATCATGACAAGGTCATGCTCTTGGGGCACAGTGATTCCTACACTCAGGACAAGAATATGCAGGTCACCATTGCATTCAACCACTTTGGAGAAGGCCTTGTTCAGAGGATGCCAAGATGTAGACATGGGAACTTTCATGTGGTGAACAATGACTACACCCATTGGGAAATGTATGCTATTGGCGGGAGTGCTTCTCCAACCATCAACAGCCAAGGCAACAGATTTCTAGCACCAAATGATAGATTCAACAAAGAGGTCACCAAGCATGAGGAAGCGCCACAGAAGGAGTGGAGCAAATGGAACTGGAGGTCCTCGGGAGATTTGCTGCTAAACGGTGCGTTCTTCACAGCATCCGGTGCCGGAGCATCTTCTAGTTACGCCAAGGCATCGAGCTTGGGTGCAAGACCGTCGTCGCTCGTGAGTTCACTCACTGCCGGAGCTGGTTCGCTCAGGTGCAAGAAGGGCTCGCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

403

Amino Acids

44.15

Weight (kDa)

8.28

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 134 - 334 1.2e-20 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013798)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67750
fragaria_vesca FvH4_4g25110
malus_domestica MD13G1106200.v1.1 MD16G1106400.v1.1
prunus_persica Prupe.1G239900_v2.0.a1 Prupe.1G239900_v2.0.a1
pyrus_communis pycom13g09220 pycom16g09060
rosa_chinensis RchiOBHm_Chr4g0432291
rosa_laevigata RLG00000006855
rosa_multiflora Rmu_sc0001084.1_g000044
rosa_roxburghii Rroxscaffold_5G00373750
rosa_rugosa Rorug04G0256800
rosa_samantha Rh4BG320700 Rh4CG335900 Rh4DG316200
rosa_wichuraiana Rw4G027220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 232
Acc36I ACCTGC 1 cut(s) 784
AccB1I GGYRCC 2 cut(s) 155, 1088
AccBSI CCGCTC 1 cut(s) 361
AccI GTMKAC 1 cut(s) 853
AccII CGCG 1 cut(s) 1201
AciI CCGC 6 cut(s) 211, 217, 261, 359, 586, 915
AclWI GGATC 5 cut(s) 22, 74, 340, 374, 651
AcsI RAATTY 1 cut(s) 133
AfaI GTAC 2 cut(s) 105, 491
AfiI CCNNNNNNNGG 3 cut(s) 199, 323, 558
AgsI TTSAA 3 cut(s) 427, 811, 979
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AloI GAACNNNNNNTCC 2 cut(s) 1025, 1057
AluBI AGCT 3 cut(s) 397, 1125, 1172
AluI AGCT 3 cut(s) 397, 1125, 1172
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 5 cut(s) 22, 74, 340, 374, 651
Ama87I CYCGRG 1 cut(s) 1045
AoxI GGCC 3 cut(s) 252, 470, 826
ApaI GGGCCC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 1057
ApoI RAATTY 1 cut(s) 133
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 2 cut(s) 233, 1009
AspS9I GGNCC 3 cut(s) 470, 471, 1041
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 6 cut(s) 103, 322, 700, 790, 886, 982
AsuII TTCGAA 1 cut(s) 659
AsuNHI GCTAGC 1 cut(s) 220
AvaI CYCGRG 1 cut(s) 1045
AvaII GGWCC 1 cut(s) 1041
BaeGI GKGCMC 2 cut(s) 474, 762
BanI GGYRCC 2 cut(s) 155, 1088
BanII GRGCYC 3 cut(s) 474, 678, 1199
BauI CACGAG 1 cut(s) 1148
BbvI GCAGC 1 cut(s) 1044
BccI CCATC 5 cut(s) 312, 440, 695, 713, 941
BceAI ACGGC 3 cut(s) 355, 454, 651
BcgI CGANNNNNNTGC 2 cut(s) 1122, 1156
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 3 cut(s) 221, 959, 1104
BfoI RGCGCY 1 cut(s) 1010
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 3 cut(s) 218, 262, 1058
BlsI GCNGC 3 cut(s) 219, 263, 1059
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 1 cut(s) 1041
BmeT110I CYCGRG 1 cut(s) 1045
BmgT120I GGNCC 3 cut(s) 470, 471, 1041
BmiI GGNNCC 6 cut(s) 157, 328, 448, 472, 697, 1090
BmrFI CCNGG 1 cut(s) 324
BmrI ACTGGG 2 cut(s) 65, 208
BmsI GCATC 8 cut(s) 84, 123, 673, 742, 831, 1091, 1106, 1127
BmtI GCTAGC 1 cut(s) 224
BmuI ACTGGG 2 cut(s) 65, 208
BoxI GACNNNNGTC 1 cut(s) 1141
BplI GAGNNNNNCTC 2 cut(s) 910, 942
BpmI CTGGAG 2 cut(s) 202, 1057
Bpu10I CCTNAGC 1 cut(s) 1181
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 324
Bsa29I ATCGAT 1 cut(s) 681
BsaAI YACGTR 3 cut(s) 283, 493, 556
BsaJI CCNNGG 5 cut(s) 511, 691, 943, 1044, 1113
BsaWI WCCGGW 3 cut(s) 158, 307, 1085
BsaXI ACNNNNNCTCC 4 cut(s) 438, 468, 594, 624
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 323, 558
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 4 cut(s) 71, 93, 203, 1040
Bse3DI GCAATG 3 cut(s) 12, 459, 801
BseCI ATCGAT 1 cut(s) 681
BseDI CCNNGG 5 cut(s) 511, 691, 943, 1044, 1113
BseGI GGATG 5 cut(s) 304, 507, 687, 846, 1082
BseLI CCNNNNNNNGG 3 cut(s) 199, 323, 558
BseMI GCAATG 3 cut(s) 12, 459, 801
BseMII CTCAG 3 cut(s) 309, 791, 1195
BseNI ACTGG 4 cut(s) 71, 93, 203, 1040
BseRI GAGGAG 3 cut(s) 29, 55, 459
BseSI GKGCMC 2 cut(s) 474, 762
BseXI GCAGC 1 cut(s) 1044
Bsh1236I CGCG 1 cut(s) 1201
BshFI GGCC 3 cut(s) 254, 472, 828
BshNI GGYRCC 2 cut(s) 155, 1088
BshVI ATCGAT 1 cut(s) 681
BsiHKCI CYCGRG 1 cut(s) 1045
BsiSI CCGG 8 cut(s) 159, 308, 324, 465, 569, 1086, 1092, 1167
BslFI GGGAC 2 cut(s) 281, 575
BslI CCNNNNNNNGG 3 cut(s) 199, 323, 558
BsmAI GTCTC 1 cut(s) 597
BsmBI CGTCTC 1 cut(s) 597
BsmFI GGGAC 2 cut(s) 281, 575
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 3 cut(s) 254, 472, 828
BsoBI CYCGRG 1 cut(s) 1045
Bsp119I TTCGAA 1 cut(s) 659
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 4 cut(s) 474, 678, 762, 1199
Bsp143I GATC 6 cut(s) 27, 79, 345, 366, 390, 643
Bsp19I CCATGG 1 cut(s) 691
BspACI CCGC 6 cut(s) 211, 217, 261, 359, 586, 915
BspANI GGCC 3 cut(s) 254, 472, 828
BspCNI CTCAG 3 cut(s) 308, 790, 1194
BspDI ATCGAT 1 cut(s) 681
BspFNI CGCG 1 cut(s) 1201
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 6 cut(s) 157, 328, 448, 472, 697, 1090
BspMI ACCTGC 1 cut(s) 784
BspOI GCTAGC 1 cut(s) 224
BspPI GGATC 5 cut(s) 22, 74, 340, 374, 651
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 2 cut(s) 155, 1088
BsrBI CCGCTC 1 cut(s) 361
BsrDI GCAATG 3 cut(s) 12, 459, 801
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 4 cut(s) 71, 93, 203, 1040
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 5 cut(s) 511, 691, 943, 1044, 1113
BssMI GATC 6 cut(s) 27, 79, 345, 366, 390, 643
BssSI CACGAG 1 cut(s) 1148
BssT1I CCWWGG 3 cut(s) 691, 943, 1113
Bst2BI CACGAG 1 cut(s) 1148
Bst4CI ACNGT 5 cut(s) 272, 489, 764, 1067, 1140
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstBAI YACGTR 3 cut(s) 283, 493, 556
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 6 cut(s) 215, 222, 252, 451, 536, 1199
BstDEI CTNAG 3 cut(s) 295, 777, 1181
BstDSI CCRYGG 2 cut(s) 511, 691
BstEII GGTNACC 2 cut(s) 796, 988
BstENI CCTNNNNNAGG 1 cut(s) 556
BstF5I GGATG 5 cut(s) 304, 507, 687, 846, 1082
BstFNI CGCG 1 cut(s) 1201
BstH2I RGCGCY 1 cut(s) 1010
BstHHI GCGC 2 cut(s) 233, 1009
BstKTI GATC 6 cut(s) 30, 82, 348, 369, 393, 646
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 6 cut(s) 27, 79, 345, 366, 390, 643
BstMWI GCNNNNNNNGC 5 cut(s) 230, 757, 1088, 1194, 1203
BstPAI GACNNNNGTC 1 cut(s) 1141
BstPI GGTNACC 2 cut(s) 796, 988
BstSCI CCNGG 1 cut(s) 322
BstSLI GKGCMC 2 cut(s) 474, 762
BstUI CGCG 1 cut(s) 1201
BstV1I GCAGC 1 cut(s) 1044
BstX2I RGATCY 1 cut(s) 79
BstYI RGATCY 1 cut(s) 79
Bsu15I ATCGAT 1 cut(s) 681
BsuRI GGCC 3 cut(s) 254, 472, 828
BsuTUI ATCGAT 1 cut(s) 681
BtgI CCRYGG 2 cut(s) 511, 691
BtsCI GGATG 5 cut(s) 304, 507, 687, 846, 1082
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 2 cut(s) 769, 1161
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 6 cut(s) 215, 222, 252, 451, 536, 1199
CciI TCATGA 1 cut(s) 736
CfoI GCGC 2 cut(s) 233, 1009
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 3 cut(s) 470, 471, 1041
ClaI ATCGAT 1 cut(s) 681
CseI GACGC 3 cut(s) 442, 598, 739
Csp6I GTAC 2 cut(s) 104, 490
CviAII CATG 9 cut(s) 385, 632, 692, 727, 737, 748, 857, 869, 998
CviQI GTAC 2 cut(s) 104, 490
DdeI CTNAG 3 cut(s) 295, 777, 1181
DpnI GATC 6 cut(s) 29, 81, 347, 368, 392, 645
DpnII GATC 6 cut(s) 27, 79, 345, 366, 390, 643
EciI GGCGGA 1 cut(s) 601
Eco130I CCWWGG 3 cut(s) 691, 943, 1113
Eco147I AGGCCT 1 cut(s) 828
Eco24I GRGCYC 3 cut(s) 474, 678, 1199
Eco47I GGWCC 1 cut(s) 1041
Eco88I CYCGRG 1 cut(s) 1045
Eco91I GGTNACC 2 cut(s) 796, 988
EcoNI CCTNNNNNAGG 1 cut(s) 556
EcoO109I RGGNCCY 2 cut(s) 471, 1041
EcoO65I GGTNACC 2 cut(s) 796, 988
EcoT14I CCWWGG 3 cut(s) 691, 943, 1113
EcoT38I GRGCYC 3 cut(s) 474, 678, 1199
ErhI CCWWGG 3 cut(s) 691, 943, 1113
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 9 cut(s) 388, 635, 695, 730, 740, 751, 860, 872, 1001
FaqI GGGAC 2 cut(s) 281, 575
FatI CATG 9 cut(s) 384, 631, 691, 726, 736, 747, 856, 868, 997
FauI CCCGC 1 cut(s) 908
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 3 cut(s) 218, 262, 1058
FokI GGATG 5 cut(s) 291, 494, 674, 853, 1069
FriOI GRGCYC 3 cut(s) 474, 678, 1199
Fsp4HI GCNGC 3 cut(s) 218, 262, 1058
FspBI CTAG 3 cut(s) 221, 959, 1104
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 2 cut(s) 232, 1008
GluI GCNGC 3 cut(s) 218, 262, 1058
GsuI CTGGAG 2 cut(s) 202, 1057
HaeII RGCGCY 1 cut(s) 1010
HaeIII GGCC 3 cut(s) 254, 472, 828
HapII CCGG 8 cut(s) 159, 308, 324, 465, 569, 1086, 1092, 1167
HgaI GACGC 3 cut(s) 442, 598, 739
HhaI GCGC 2 cut(s) 233, 1009
Hin1II CATG 9 cut(s) 388, 635, 695, 730, 740, 751, 860, 872, 1001
Hin6I GCGC 2 cut(s) 231, 1007
HinP1I GCGC 2 cut(s) 231, 1007
HinfI GANTC 4 cut(s) 293, 563, 767, 975
HpaII CCGG 8 cut(s) 159, 308, 324, 465, 569, 1086, 1092, 1167
HphI GGTGA 6 cut(s) 103, 322, 700, 790, 886, 982
Hpy166II GTNNAC 5 cut(s) 313, 457, 854, 877, 1157
Hpy188I TCNGA 4 cut(s) 245, 335, 598, 837
Hpy188III TCNNGA 8 cut(s) 83, 364, 427, 527, 737, 779, 1047, 1150
Hpy8I GTNNAC 5 cut(s) 313, 457, 854, 877, 1157
Hpy99I CGWCG 4 cut(s) 440, 602, 1144, 1147
HpyAV CCTTC 4 cut(s) 433, 818, 1009, 1186
HpyCH4III ACNGT 5 cut(s) 272, 489, 764, 1067, 1140
HpyCH4IV ACGT 3 cut(s) 282, 492, 555
HpyCH4V TGCA 7 cut(s) 97, 477, 534, 793, 806, 1133, 1188
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 757, 1088, 1194, 1203
HpyF3I CTNAG 3 cut(s) 295, 777, 1181
HpySE526I ACGT 3 cut(s) 282, 492, 555
Hsp92II CATG 9 cut(s) 388, 635, 695, 730, 740, 751, 860, 872, 1001
HspAI GCGC 2 cut(s) 231, 1007
Kzo9I GATC 6 cut(s) 27, 79, 345, 366, 390, 643
LmnI GCTCC 5 cut(s) 446, 571, 1023, 1094, 1169
Lsp1109I GCAGC 1 cut(s) 1044
LweI GCATC 8 cut(s) 84, 123, 673, 742, 831, 1091, 1106, 1127
MaeI CTAG 3 cut(s) 221, 959, 1104
MaeII ACGT 3 cut(s) 282, 492, 555
MaeIII GTNAC 8 cut(s) 188, 286, 380, 493, 668, 796, 988, 1106
MalI GATC 6 cut(s) 29, 81, 347, 368, 392, 645
MbiI CCGCTC 1 cut(s) 361
MboI GATC 6 cut(s) 27, 79, 345, 366, 390, 643
MboII GAAGA 4 cut(s) 217, 416, 1066, 1092
MflI RGATCY 1 cut(s) 79
MhlI GDGCHC 4 cut(s) 474, 678, 762, 1199
MluCI AATT 1 cut(s) 133
MlyI GAGTC 2 cut(s) 287, 557
MmeI TCCRAC 2 cut(s) 621, 953
MroXI GAANNNNTTC 1 cut(s) 422
MseI TTAA 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 8 cut(s) 159, 308, 324, 465, 569, 1086, 1092, 1167
MspR9I CCNGG 1 cut(s) 324
Mva1269I GAATGC 1 cut(s) 806
MvnI CGCG 1 cut(s) 1201
MwoI GCNNNNNNNGC 5 cut(s) 230, 757, 1088, 1194, 1203
NciI CCSGG 1 cut(s) 324
NcoI CCATGG 1 cut(s) 691
NdeII GATC 6 cut(s) 27, 79, 345, 366, 390, 643
NheI GCTAGC 1 cut(s) 220
NlaIII CATG 9 cut(s) 388, 635, 695, 730, 740, 751, 860, 872, 1001
NlaIV GGNNCC 6 cut(s) 157, 328, 448, 472, 697, 1090
NmuCI GTSAC 7 cut(s) 188, 286, 380, 493, 668, 796, 988
NsbI TGCGCA 1 cut(s) 232
NspV TTCGAA 1 cut(s) 659
PagI TCATGA 1 cut(s) 736
PceI AGGCCT 1 cut(s) 828
PcsI WCGNNNNNNNCGW 1 cut(s) 678
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 2 cut(s) 767, 975
PflFI GACNNNGTC 1 cut(s) 743
PkrI GCNGC 3 cut(s) 219, 263, 1059
PleI GAGTC 2 cut(s) 287, 557
PpsI GAGTC 2 cut(s) 287, 557
Ppu21I YACGTR 3 cut(s) 283, 493, 556
PpuMI RGGWCCY 1 cut(s) 1041
PshAI GACNNNNGTC 1 cut(s) 1141
Psp5II RGGWCCY 1 cut(s) 1041
PspEI GGTNACC 2 cut(s) 796, 988
PspN4I GGNNCC 6 cut(s) 157, 328, 448, 472, 697, 1090
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 3 cut(s) 470, 471, 1041
PspPPI RGGWCCY 1 cut(s) 1041
PsrI GAACNNNNNNTAC 2 cut(s) 869, 901
PsuI RGATCY 1 cut(s) 79
PsyI GACNNNGTC 1 cut(s) 743
RsaI GTAC 2 cut(s) 105, 491
RsaNI GTAC 2 cut(s) 104, 490
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 3 cut(s) 218, 262, 1058
Sau3AI GATC 6 cut(s) 27, 79, 345, 366, 390, 643
Sau96I GGNCC 3 cut(s) 470, 471, 1041
SchI GAGTC 2 cut(s) 287, 557
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 4 cut(s) 474, 678, 762, 1199
SfaNI GCATC 8 cut(s) 84, 123, 673, 742, 831, 1091, 1106, 1127
SfuI TTCGAA 1 cut(s) 659
SinI GGWCC 1 cut(s) 1041
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 1 cut(s) 133
SseBI AGGCCT 1 cut(s) 828
SsiI CCGC 6 cut(s) 211, 217, 261, 359, 586, 915
SspMI CTAG 3 cut(s) 221, 959, 1104
StuI AGGCCT 1 cut(s) 828
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 3 cut(s) 691, 943, 1113
TaaI ACNGT 5 cut(s) 272, 489, 764, 1067, 1140
TaiI ACGT 3 cut(s) 285, 495, 558
TaqI TCGA 4 cut(s) 435, 659, 681, 1121
TasI AATT 1 cut(s) 133
TatI WGTACW 1 cut(s) 103
TauI GCSGC 2 cut(s) 220, 264
TfiI GAWTC 2 cut(s) 767, 975
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TscAI CASTG 2 cut(s) 769, 1168
TseFI GTSAC 7 cut(s) 188, 286, 380, 493, 668, 796, 988
TseI GCWGC 1 cut(s) 1057
Tsp45I GTSAC 7 cut(s) 188, 286, 380, 493, 668, 796, 988
TspDTI ATGAA 2 cut(s) 431, 857
TspGWI ACGGA 3 cut(s) 305, 528, 615
TspRI CASTG 2 cut(s) 769, 1168
Tth111I GACNNNGTC 1 cut(s) 743
VpaK11BI GGWCC 1 cut(s) 1041
XagI CCTNNNNNAGG 1 cut(s) 556
XapI RAATTY 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 2 cut(s) 464, 1017
XmiI GTMKAC 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 3 cut(s) 221, 959, 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.