FvH4_4g25110

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013798
Physical Location & Seq
Forward (+)
27012326 .. 27013842
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25110.t1

Sequence Viewer

Length: 1209 bp
ATGGCAATGCCACTCTCTCTTCTCTCCCTCCTCTCTCTCCTCCTCTTCCCATCTCTCATTTCGTCCTCCCCGTTCCCAAACCCCGATTCCGTAATCCAAGAAGTACACAGGAGCATTAATGCTTCAAGGAGGAGTTTGGGTTATCTTTCTTGCGGAAGCGGAAACCCCATCGACGATTGCTGGAGGTGCGACCCCAAATGGGAGCAGAACCGCCAGCGACTCGCCGACTGCGCAATCGGGTTCGGAAAGCACGCCATTGGCGGCAGAGACGGCAAAATCTACGTCGTCACCGACGCCGGCGACCACCCTGTCAACCCAAAACCCGGGACTCTCCGTTACGGCGTCATCCAAGACGAGCCTTTATGGATCATATTCAAAGGTGACATGACCATAAAGCTGAAGGAGGAGCTGATGATGAACTCGTTCAAGACCATCGACGGCAGAGGAGCCAATGTACACATCGCCGGCGGGCCTTGCATTACTATTCAGTACGTGACGAATATCATCATCCATGGTTTGAACATCCACGACTGCAAGCAAGGCGGGAATGCTTATGTTAGAGACTCTCCGGGGCATTATGGGTGGAGGACTTTGTCTGACGGAGACGGGGTTTCGATTTTCGGGGGAAGCCATGTTTGGGTGGATCATTGCTCTCTTTCAAACTGCCGTGATGGACTGATTGATGCTATTCATGGATCCACGGCTATTACGATTTCGAACAATTATATGACGCACCATAACAAGGTCATGTTGTTGGGTCACAGTGATAGTTACACGCAGGACAAGAACATGCAGGTCACCATTGCGTTCAATCACTTTGGAGAAGGCCTTGTTCAGAGGATGCCAAGGTGTAGGCATGGAAATTTTCATGTGGTGAACAATGACTATACACATTGGGAAATGTATGCAATTGGGGGTAGTGCTTCTCCAACAATCAACAGCCAAGGGAATAGGTTTCTTGCACCTAATGATAGATTCAACAAAGAGGTGACTAAACATGAGGATGCACCACAGAAAGACTGGAGCAAATGGAACTGGAGGTCTTCAGGGGACTTAATGCTGAATGGTGCATTTTTCACAGCGTCCGGTGCCGGAGCTTCTTCCAGCTACGCCAAGGCGTCGAGTTTGGGTGCAAGGCCATCTTCCCTAGTGAGCTCAATCACAGCTGGTGCTGGTTCACTCAAATGTAAGAAAGGCTCGCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

403

Amino Acids

43.94

Weight (kDa)

8.57

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 134 - 345 9.6e-22 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013798)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67750
fragaria_vesca FvH4_4g25110
malus_domestica MD13G1106200.v1.1 MD16G1106400.v1.1
prunus_persica Prupe.1G239900_v2.0.a1 Prupe.1G239900_v2.0.a1
pyrus_communis pycom13g09220 pycom16g09060
rosa_chinensis RchiOBHm_Chr4g0432291
rosa_laevigata RLG00000006855
rosa_multiflora Rmu_sc0001084.1_g000044
rosa_roxburghii Rroxscaffold_5G00373750
rosa_rugosa Rorug04G0256800
rosa_samantha Rh4BG320700 Rh4CG335900 Rh4DG316200
rosa_wichuraiana Rw4G027220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
Acc16I TGCGCA 1 cut(s) 232
Acc36I ACCTGC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 1088
AccII CGCG 1 cut(s) 1201
AciI CCGC 6 cut(s) 153, 159, 211, 261, 468, 543
AclWI GGATC 4 cut(s) 374, 651, 690, 703
AcsI RAATTY 1 cut(s) 862
AcuI CTGAAG 2 cut(s) 419, 1029
AcyI GRCGYC 3 cut(s) 294, 342, 1118
AfaI GTAC 3 cut(s) 105, 456, 491
AfiI CCNNNNNNNGG 4 cut(s) 199, 323, 637, 742
AgsI TTSAA 7 cut(s) 126, 376, 427, 520, 660, 811, 979
AjuI GAANNNNNNNTTGG 4 cut(s) 70, 102, 619, 651
AluBI AGCT 6 cut(s) 397, 409, 1097, 1107, 1155, 1166
AluI AGCT 6 cut(s) 397, 409, 1097, 1107, 1155, 1166
Alw21I GWGCWC 1 cut(s) 1157
Alw26I GTCTC 3 cut(s) 261, 555, 597
AlwI GGATC 4 cut(s) 374, 651, 690, 703
Ama87I CYCGRG 1 cut(s) 323
AoxI GGCC 3 cut(s) 470, 826, 1136
ApoI RAATTY 1 cut(s) 862
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AseI ATTAAT 1 cut(s) 117
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 470
AsuC2I CCSGG 3 cut(s) 324, 325, 570
AsuHPI GGTGA 5 cut(s) 280, 392, 790, 886, 1000
AsuII TTCGAA 1 cut(s) 716
AvaI CYCGRG 1 cut(s) 323
BamHI GGATCC 1 cut(s) 695
BanI GGYRCC 1 cut(s) 1088
BanII GRGCYC 1 cut(s) 1157
BbsI GAAGAC 1 cut(s) 1035
Bbv12I GWGCWC 1 cut(s) 1157
BccI CCATC 5 cut(s) 58, 176, 440, 665, 1147
BceAI ACGGC 5 cut(s) 286, 355, 454, 651, 717
BcnI CCSGG 3 cut(s) 324, 325, 570
BcoDI GTCTC 3 cut(s) 261, 555, 597
BfaI CTAG 1 cut(s) 1148
BfuAI ACCTGC 1 cut(s) 784
BisI GCNGC 1 cut(s) 262
BlsI GCNGC 1 cut(s) 263
Bme1390I CCNGG 3 cut(s) 324, 325, 570
BmeT110I CYCGRG 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 3 cut(s) 448, 697, 1090
BmrFI CCNGG 3 cut(s) 324, 325, 570
BmsI GCATC 3 cut(s) 673, 831, 994
BpiI GAAGAC 1 cut(s) 1035
BpmI CTGGAG 3 cut(s) 202, 1042, 1057
Bpu14I TTCGAA 1 cut(s) 716
BpuMI CCSGG 3 cut(s) 324, 325, 570
BsaAI YACGTR 1 cut(s) 493
BsaHI GRCGYC 3 cut(s) 294, 342, 1118
BsaJI CCNNGG 7 cut(s) 323, 511, 569, 699, 845, 943, 1113
BsaWI WCCGGW 1 cut(s) 1085
BsaXI ACNNNNNCTCC 6 cut(s) 124, 154, 438, 468, 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 323, 637, 742
Bse118I RCCGGY 2 cut(s) 296, 464
Bse1I ACTGG 2 cut(s) 1025, 1040
Bse3DI GCAATG 3 cut(s) 12, 646, 801
BseDI CCNNGG 7 cut(s) 323, 511, 569, 699, 845, 943, 1113
BseGI GGATG 5 cut(s) 345, 507, 522, 846, 1009
BseLI CCNNNNNNNGG 4 cut(s) 199, 323, 637, 742
BseMI GCAATG 3 cut(s) 12, 646, 801
BseNI ACTGG 2 cut(s) 1025, 1040
BseRI GAGGAG 6 cut(s) 20, 29, 32, 145, 419, 459
Bsh1236I CGCG 1 cut(s) 1201
BshFI GGCC 3 cut(s) 472, 828, 1138
BshNI GGYRCC 1 cut(s) 1088
BsiHKAI GWGCWC 1 cut(s) 1157
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 6 cut(s) 297, 324, 465, 569, 1086, 1092
BslFI GGGAC 2 cut(s) 340, 1064
BslI CCNNNNNNNGG 4 cut(s) 199, 323, 637, 742
BsmAI GTCTC 3 cut(s) 261, 555, 597
BsmBI CGTCTC 2 cut(s) 261, 597
BsmFI GGGAC 2 cut(s) 340, 1064
BsmI GAATGC 1 cut(s) 553
BsnI GGCC 3 cut(s) 472, 828, 1138
BsoBI CYCGRG 1 cut(s) 323
Bsp119I TTCGAA 1 cut(s) 716
Bsp1286I GDGCHC 1 cut(s) 1157
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 3 cut(s) 366, 643, 695
Bsp19I CCATGG 1 cut(s) 511
BspACI CCGC 6 cut(s) 153, 159, 211, 261, 468, 543
BspANI GGCC 3 cut(s) 472, 828, 1138
BspFNI CGCG 1 cut(s) 1201
BspLI GGNNCC 3 cut(s) 448, 697, 1090
BspMI ACCTGC 1 cut(s) 784
BspPI GGATC 4 cut(s) 374, 651, 690, 703
BspT104I TTCGAA 1 cut(s) 716
BspT107I GGYRCC 1 cut(s) 1088
BsrDI GCAATG 3 cut(s) 12, 646, 801
BsrFI RCCGGY 2 cut(s) 296, 464
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 2 cut(s) 1025, 1040
BssAI RCCGGY 2 cut(s) 296, 464
BssECI CCNNGG 7 cut(s) 323, 511, 569, 699, 845, 943, 1113
BssMI GATC 3 cut(s) 366, 643, 695
BssNI GRCGYC 3 cut(s) 294, 342, 1118
BssT1I CCWWGG 4 cut(s) 511, 845, 943, 1113
Bst4CI ACNGT 1 cut(s) 764
Bst6I CTCTTC 2 cut(s) 24, 50
BstACI GRCGYC 3 cut(s) 294, 342, 1118
BstAUI TGTACA 1 cut(s) 454
BstBAI YACGTR 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 716
BstC8I GCNNGC 7 cut(s) 215, 252, 298, 466, 470, 536, 1199
BstDSI CCRYGG 2 cut(s) 511, 699
BstEII GGTNACC 1 cut(s) 796
BstF5I GGATG 5 cut(s) 345, 507, 522, 846, 1009
BstFNI CGCG 1 cut(s) 1201
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 3 cut(s) 369, 646, 698
BstMAI GTCTC 3 cut(s) 261, 555, 597
BstMBI GATC 3 cut(s) 366, 643, 695
BstMWI GCNNNNNNNGC 7 cut(s) 186, 230, 270, 474, 540, 1088, 1203
BstNSI RCATGY 1 cut(s) 793
BstPI GGTNACC 1 cut(s) 796
BstSCI CCNGG 3 cut(s) 322, 323, 568
BstUI CGCG 1 cut(s) 1201
BstV2I GAAGAC 1 cut(s) 1035
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
BsuRI GGCC 3 cut(s) 472, 828, 1138
BtgI CCRYGG 2 cut(s) 511, 699
BtgZI GCGATG 1 cut(s) 445
BtsCI GGATG 5 cut(s) 345, 507, 522, 846, 1009
BtsIMutI CAGTG 1 cut(s) 769
BveI ACCTGC 1 cut(s) 784
Cac8I GCNNGC 7 cut(s) 215, 252, 298, 466, 470, 536, 1199
CfoI GCGC 1 cut(s) 233
Cfr10I RCCGGY 2 cut(s) 296, 464
Cfr13I GGNCC 1 cut(s) 470
Cfr9I CCCGGG 1 cut(s) 323
CseI GACGC 5 cut(s) 302, 331, 739, 1071, 1107
Csp6I GTAC 3 cut(s) 104, 455, 490
CviAII CATG 9 cut(s) 385, 512, 632, 692, 748, 790, 857, 869, 998
CviQI GTAC 3 cut(s) 104, 455, 490
DpnI GATC 3 cut(s) 368, 645, 697
DpnII GATC 3 cut(s) 366, 643, 695
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 2 cut(s) 24, 50
EarI CTCTTC 2 cut(s) 24, 50
Ecl136II GAGCTC 1 cut(s) 1155
Eco130I CCWWGG 4 cut(s) 511, 845, 943, 1113
Eco147I AGGCCT 1 cut(s) 828
Eco24I GRGCYC 1 cut(s) 1157
Eco53kI GAGCTC 1 cut(s) 1155
Eco57I CTGAAG 2 cut(s) 419, 1029
Eco88I CYCGRG 1 cut(s) 323
Eco91I GGTNACC 1 cut(s) 796
EcoICRI GAGCTC 1 cut(s) 1155
EcoO65I GGTNACC 1 cut(s) 796
EcoT14I CCWWGG 4 cut(s) 511, 845, 943, 1113
EcoT38I GRGCYC 1 cut(s) 1157
ErhI CCWWGG 4 cut(s) 511, 845, 943, 1113
Esp3I CGTCTC 2 cut(s) 261, 597
FaeI CATG 9 cut(s) 388, 515, 635, 695, 751, 793, 860, 872, 1001
FalI AAGNNNNNCTT 2 cut(s) 1126, 1158
FaqI GGGAC 2 cut(s) 340, 1064
FatI CATG 9 cut(s) 384, 511, 631, 691, 747, 789, 856, 868, 997
FauI CCCGC 2 cut(s) 461, 536
Fnu4HI GCNGC 1 cut(s) 262
FokI GGATG 5 cut(s) 332, 494, 509, 853, 1016
FriOI GRGCYC 1 cut(s) 1157
Fsp4HI GCNGC 1 cut(s) 262
FspBI CTAG 1 cut(s) 1148
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 1 cut(s) 232
GluI GCNGC 1 cut(s) 262
GsuI CTGGAG 3 cut(s) 202, 1042, 1057
HaeIII GGCC 3 cut(s) 472, 828, 1138
HapII CCGG 6 cut(s) 297, 324, 465, 569, 1086, 1092
HgaI GACGC 5 cut(s) 302, 331, 739, 1071, 1107
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 3 cut(s) 294, 342, 1118
Hin1II CATG 9 cut(s) 388, 515, 635, 695, 751, 793, 860, 872, 1001
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 5 cut(s) 86, 219, 328, 563, 975
HpaII CCGG 6 cut(s) 297, 324, 465, 569, 1086, 1092
HphI GGTGA 5 cut(s) 280, 392, 790, 886, 1000
Hpy166II GTNNAC 5 cut(s) 106, 313, 457, 877, 1178
Hpy188I TCNGA 3 cut(s) 245, 598, 837
Hpy188III TCNNGA 1 cut(s) 427
Hpy8I GTNNAC 5 cut(s) 106, 313, 457, 877, 1178
Hpy99I CGWCG 5 cut(s) 176, 287, 296, 440, 1123
HpyAV CCTTC 2 cut(s) 394, 818
HpyCH4III ACNGT 1 cut(s) 764
HpyCH4IV ACGT 2 cut(s) 282, 492
HpyCH4V TGCA 8 cut(s) 477, 534, 793, 908, 962, 1007, 1070, 1133
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 230, 270, 474, 540, 1088, 1203
HpySE526I ACGT 2 cut(s) 282, 492
Hsp92I GRCGYC 3 cut(s) 294, 342, 1118
Hsp92II CATG 9 cut(s) 388, 515, 635, 695, 751, 793, 860, 872, 1001
HspAI GCGC 1 cut(s) 231
KroI GCCGGC 2 cut(s) 296, 464
KroNI GCCGGC 2 cut(s) 298, 466
Kzo9I GATC 3 cut(s) 366, 643, 695
LmnI GCTCC 6 cut(s) 111, 202, 406, 446, 1023, 1094
LweI GCATC 3 cut(s) 673, 831, 994
MaeI CTAG 1 cut(s) 1148
MaeII ACGT 2 cut(s) 282, 492
MaeIII GTNAC 8 cut(s) 286, 335, 380, 493, 758, 770, 796, 988
MalI GATC 3 cut(s) 368, 645, 697
MboI GATC 3 cut(s) 366, 643, 695
MboII GAAGA 5 cut(s) 11, 37, 1035, 1092, 1134
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 1157
MluCI AATT 3 cut(s) 721, 862, 909
MlyI GAGTC 3 cut(s) 213, 322, 557
MmeI TCCRAC 1 cut(s) 953
MreI CGCCGGCG 2 cut(s) 296, 464
MroNI GCCGGC 2 cut(s) 296, 464
MroXI GAANNNNTTC 1 cut(s) 422
MseI TTAA 2 cut(s) 117, 1055
MslI CAYNNNNRTG 2 cut(s) 1002, 1183
MspA1I CMGCKG 1 cut(s) 1166
MspI CCGG 6 cut(s) 297, 324, 465, 569, 1086, 1092
MspR9I CCNGG 3 cut(s) 324, 325, 570
MunI CAATTG 1 cut(s) 909
Mva1269I GAATGC 1 cut(s) 553
MvnI CGCG 1 cut(s) 1201
MwoI GCNNNNNNNGC 7 cut(s) 186, 230, 270, 474, 540, 1088, 1203
NaeI GCCGGC 2 cut(s) 298, 466
NciI CCSGG 3 cut(s) 324, 325, 570
NcoI CCATGG 1 cut(s) 511
NdeII GATC 3 cut(s) 366, 643, 695
NgoMIV GCCGGC 2 cut(s) 296, 464
NlaIII CATG 9 cut(s) 388, 515, 635, 695, 751, 793, 860, 872, 1001
NlaIV GGNNCC 3 cut(s) 448, 697, 1090
NmuCI GTSAC 6 cut(s) 286, 380, 493, 758, 796, 988
NsbI TGCGCA 1 cut(s) 232
NspI RCATGY 1 cut(s) 793
NspV TTCGAA 1 cut(s) 716
PceI AGGCCT 1 cut(s) 828
PcsI WCGNNNNNNNCGW 3 cut(s) 68, 288, 707
PctI GAATGC 1 cut(s) 553
PdiI GCCGGC 2 cut(s) 298, 466
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 2 cut(s) 86, 975
PflFI GACNNNGTC 1 cut(s) 592
PkrI GCNGC 1 cut(s) 263
PleI GAGTC 3 cut(s) 213, 322, 557
PpsI GAGTC 3 cut(s) 213, 322, 557
Ppu21I YACGTR 1 cut(s) 493
PshBI ATTAAT 1 cut(s) 117
Psp124BI GAGCTC 1 cut(s) 1157
PspEI GGTNACC 1 cut(s) 796
PspN4I GGNNCC 3 cut(s) 448, 697, 1090
PspPI GGNCC 1 cut(s) 470
PsuI RGATCY 1 cut(s) 695
PsyI GACNNNGTC 1 cut(s) 592
PvuII CAGCTG 1 cut(s) 1166
RsaI GTAC 3 cut(s) 105, 456, 491
RsaNI GTAC 3 cut(s) 104, 455, 490
RseI CAYNNNNRTG 2 cut(s) 1002, 1183
SacI GAGCTC 1 cut(s) 1157
SaqAI TTAA 2 cut(s) 117, 1055
SatI GCNGC 1 cut(s) 262
Sau3AI GATC 3 cut(s) 366, 643, 695
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 3 cut(s) 213, 322, 557
ScrFI CCNGG 3 cut(s) 324, 325, 570
SduI GDGCHC 1 cut(s) 1157
SfaNI GCATC 3 cut(s) 673, 831, 994
SfuI TTCGAA 1 cut(s) 716
SgrAI CRCCGGYG 2 cut(s) 296, 464
SmaI CCCGGG 1 cut(s) 325
SmiMI CAYNNNNRTG 2 cut(s) 1002, 1183
Sse9I AATT 3 cut(s) 721, 862, 909
SseBI AGGCCT 1 cut(s) 828
SsiI CCGC 6 cut(s) 153, 159, 211, 261, 468, 543
SspMI CTAG 1 cut(s) 1148
SstI GAGCTC 1 cut(s) 1157
StuI AGGCCT 1 cut(s) 828
StyD4I CCNGG 3 cut(s) 322, 323, 568
StyI CCWWGG 4 cut(s) 511, 845, 943, 1113
TaaI ACNGT 1 cut(s) 764
TaiI ACGT 2 cut(s) 285, 495
TaqI TCGA 5 cut(s) 171, 435, 614, 716, 1121
TasI AATT 3 cut(s) 721, 862, 909
TatI WGTACW 2 cut(s) 103, 454
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 2 cut(s) 86, 975
Tru1I TTAA 2 cut(s) 117, 1055
Tru9I TTAA 2 cut(s) 117, 1055
TscAI CASTG 1 cut(s) 769
TseFI GTSAC 6 cut(s) 286, 380, 493, 758, 796, 988
Tsp45I GTSAC 6 cut(s) 286, 380, 493, 758, 796, 988
TspDTI ATGAA 3 cut(s) 431, 680, 857
TspGWI ACGGA 3 cut(s) 79, 323, 615
TspMI CCCGGG 1 cut(s) 323
TspRI CASTG 1 cut(s) 769
Tth111I GACNNNGTC 1 cut(s) 592
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 1 cut(s) 862
XceI RCATGY 1 cut(s) 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 1017
XmaI CCCGGG 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.