AT1G74030

Enolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012756
Physical Location & Seq
Reverse (-)
27839159 .. 27842132
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74030.1

Sequence Viewer

Length: 1434 bp
ATGGCTTTGACTACAAAACCTCACCATCTTCAGAGATCTTTCCTCTCTCCGTCGCGTGTTTCCGGCGAACGATATTTGGAATCTGCGCCATCATGTCTGAGATTCCGTCGCAGCGGTGTCCAATGCTCGGTGGTGGCTAAGGAGTGCAGAGTGAAAGGAGTGAAAGCGAGACAGATTATTGATAGTAGAGGGAATCCGACGGTGGAGGTTGATCTGATTACCGATGATCTGTATCGTTCGGCTGTTCCAAGTGGTGCATCTACCGGGATCTACGAAGCGCTTGAGCTTAGAGATGGAGACAAGAGCGTCTATGGTGGTAAAGGTGTATTACAGGCTATTAAAAACATCAATGAACTTGTGGCTCCAAAACTCATTGGAGTTGACGTTAGGAACCAAGCTGATGTCGATGCTCTTATGCTGGAACTGGATGGGACCCCAAACAAGTCGAAACTCGGGGCTAATGCGATATTAGGAGTGTCATTGAGCGTTTGCAGGGCAGGTGCTGGAGCTAAAGGAGTGCCTTTGTACAAACACATCCAGGAAACATCAGGAACAAAGGAGCTTGTCATGCCAGTTCCTGCATTCAATGTGATCAATGGAGGCAGTCATGCTGGGAATAGTTTGGCTATGCAAGAGTTTATGATACTACCTGTAGGAGCTACCTCATTCTCGGAGGCCTTCCAGATGGGAAGTGAAGTTTATCATACATTGAAGGGGATAATCAAAACTAAGTATGGTCAAGATGCTTGTAATGTCGGAGATGAAGGAGGGTTTGCGCCGAATGTTCAAGATAACAGAGAGGGACTAGTTCTGCTCATAGATGCAATTGAAAAGGCCGGTTACACTGGAAAGATCAAAATAGGAATGGATGTTGCTGCATCAGAATTTTTCATGAAAGATGGTAGATACGATTTGAACTTCAAGAAACAGCCAAACGATGGAGCTCACGTACTGTCAGCCGAGAGTCTTGCTGACCTCTACAGAGAATTCATCAAGGATTTCCCAATTGTCTCTATCGAAGATCCTTTTGACCAGGATGATTGGAGCTCATGGGCTTCATTGCAATCCTCTGTGGATATCCAACTCGTGGGAGATGACTTGTTAGTCACTAACCCGAAGAGGATAGCTGAAGCTATTAAGAAACAGTCTTGCAATGCTCTACTCTTGAAGGTTAACCAGATTGGGACAGTCACTGAGTCAATTCAAGCAGCACTTGACTCAAAAGCTGCAGGCTGGGGTGTGATGGTTAGTCACAGGAGTGGCGAGACAGAGGATAACTTCATCGCAGATCTCTCTGTTGGTTTAGCAAGCGGACAGATCAAAACTGGTGCTCCATGCCGAAGTGAACGATTGTCAAAATACAACCAGCTTCTCCGTATCGAAGAGGAACTCGGCAATGTGCGCTACGCCGGTGAAGCTTTCCGATCACCATGA

Protein Analysis

477

Amino Acids

51.47

Weight (kDa)

5.79

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Enolase_N PF03952 52 - 179 1e-55 Enolase, N-terminal domain
Enolase_C PF00113 188 - 475 2.4e-146 Enolase, C-terminal TIM barrel domain
MR_MLE_C PF13378 317 - 438 1.1e-07 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012756)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74030
fragaria_vesca FvH4_5g12990 FvH4_5g12990 FvH4_5g12990
malus_domestica MD06G1208300.v1.1 MD14G1218700.v1.1
prunus_persica Prupe.5G212800_v2.0.a1
pyrus_communis pycom06g18610
rosa_chinensis RchiOBHm_Chr7g0184181
rosa_laevigata RLG00000004996
rosa_multiflora Rmu_sc0012221.1_g000006
rosa_roxburghii Rroxscaffold_3G00269820
rosa_rugosa Rorug06G0463700
rosa_samantha Rh7AG082200 Rh7BG065000 Rh7CG065400 Rh7DG065100
rosa_wichuraiana Rw7G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 488
AasI GACNNNNNNGTC 2 cut(s) 305, 1103
Acc36I ACCTGC 1 cut(s) 488
AccB7I CCANNNNNTGG 2 cut(s) 938, 1087
AccII CGCG 1 cut(s) 55
AciI CCGC 2 cut(s) 114, 1311
AclWI GGATC 2 cut(s) 275, 1016
AcsI RAATTY 2 cut(s) 884, 986
AcuI CTGAAG 2 cut(s) 14, 1149
AfaI GTAC 2 cut(s) 527, 951
AfeI AGCGCT 1 cut(s) 279
AfiI CCNNNNNNNGG 3 cut(s) 127, 938, 1087
AgsI TTSAA 8 cut(s) 586, 712, 788, 830, 916, 922, 1168, 1205
AhlI ACTAGT 1 cut(s) 805
AjnI CCWGG 2 cut(s) 537, 1032
AleI CACNNNNGTG 1 cut(s) 1257
Alw21I GWGCWC 3 cut(s) 946, 1049, 1333
Alw26I GTCTC 4 cut(s) 163, 291, 1015, 1259
AlwI GGATC 2 cut(s) 275, 1016
AlwNI CAGNNNCTG 3 cut(s) 503, 578, 1193
Ama87I CYCGRG 1 cut(s) 452
Aor51HI AGCGCT 1 cut(s) 279
AoxI GGCC 2 cut(s) 675, 834
ApeKI GCWGC 4 cut(s) 111, 875, 1208, 1226
ApoI RAATTY 2 cut(s) 884, 986
AspLEI GCGC 4 cut(s) 88, 280, 778, 1404
AspS9I GGNCC 1 cut(s) 432
AsuC2I CCSGG 1 cut(s) 265
AsuHPI GGTGA 3 cut(s) 14, 1419, 1424
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 432
BanII GRGCYC 2 cut(s) 946, 1049
BauI CACGAG 1 cut(s) 1085
Bbv12I GWGCWC 3 cut(s) 946, 1049, 1333
BbvI GCAGC 4 cut(s) 123, 862, 1213, 1220
BccI CCATC 8 cut(s) 33, 97, 287, 422, 679, 893, 932, 1237
BcgI CGANNNNNNTGC 2 cut(s) 950, 984
BciT130I CCWGG 2 cut(s) 539, 1034
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 1 cut(s) 265
BcoDI GTCTC 4 cut(s) 163, 291, 1015, 1259
BcuI ACTAGT 1 cut(s) 805
BfaI CTAG 1 cut(s) 806
BfmI CTRYAG 3 cut(s) 651, 979, 1227
BfoI RGCGCY 1 cut(s) 281
BfuAI ACCTGC 1 cut(s) 488
BglII AGATCT 2 cut(s) 35, 1288
BisI GCNGC 4 cut(s) 112, 876, 1209, 1227
BlsI GCNGC 4 cut(s) 113, 877, 1210, 1228
Bme1390I CCNGG 3 cut(s) 265, 539, 1034
Bme18I GGWCC 1 cut(s) 432
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 4 cut(s) 363, 392, 433, 434
BmrFI CCNGG 3 cut(s) 265, 539, 1034
BmsI GCATC 5 cut(s) 266, 397, 733, 811, 887
BpmI CTGGAG 1 cut(s) 525
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 265
BsaAI YACGTR 1 cut(s) 949
BsaBI GATNNNNATC 1 cut(s) 231
BsaXI ACNNNNNCTCC 6 cut(s) 369, 399, 933, 963, 1315, 1345
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 938, 1087
Bse118I RCCGGY 2 cut(s) 836, 1409
Bse1I ACTGG 4 cut(s) 429, 572, 850, 1330
Bse3DI GCAATG 3 cut(s) 1058, 1159, 1402
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 2 cut(s) 539, 1034
BseGI GGATG 4 cut(s) 433, 534, 874, 1042
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 3 cut(s) 127, 938, 1087
BseMI GCAATG 3 cut(s) 1058, 1159, 1402
BseMII CTCAG 2 cut(s) 89, 1185
BseNI ACTGG 4 cut(s) 429, 572, 850, 1330
BseXI GCAGC 4 cut(s) 123, 862, 1213, 1220
BseYI CCCAGC 2 cut(s) 611, 1233
BsgI GTGCAG 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 2 cut(s) 677, 836
BsiHKAI GWGCWC 3 cut(s) 946, 1049, 1333
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 4 cut(s) 63, 264, 837, 1410
BslFI GGGAC 3 cut(s) 445, 816, 1198
BslI CCNNNNNNNGG 3 cut(s) 127, 938, 1087
BsmAI GTCTC 4 cut(s) 163, 291, 1015, 1259
BsmFI GGGAC 3 cut(s) 445, 816, 1198
BsmI GAATGC 1 cut(s) 581
BsnI GGCC 2 cut(s) 677, 836
BsoBI CYCGRG 1 cut(s) 452
Bsp1286I GDGCHC 3 cut(s) 946, 1049, 1333
Bsp1407I TGTACA 1 cut(s) 525
BspACI CCGC 2 cut(s) 114, 1311
BspANI GGCC 2 cut(s) 677, 836
BspCNI CTCAG 2 cut(s) 90, 1186
BspFNI CGCG 1 cut(s) 55
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 4 cut(s) 363, 392, 433, 434
BspMAI CTGCAG 1 cut(s) 1231
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 2 cut(s) 275, 1016
BsrDI GCAATG 3 cut(s) 1058, 1159, 1402
BsrFI RCCGGY 2 cut(s) 836, 1409
BsrGI TGTACA 1 cut(s) 525
BsrI ACTGG 4 cut(s) 429, 572, 850, 1330
BssAI RCCGGY 2 cut(s) 836, 1409
BssSI CACGAG 1 cut(s) 1085
Bst2BI CACGAG 1 cut(s) 1085
Bst2UI CCWGG 2 cut(s) 539, 1034
Bst4CI ACNGT 4 cut(s) 202, 954, 1146, 1189
Bst6I CTCTTC 2 cut(s) 1112, 1377
BstAUI TGTACA 1 cut(s) 525
BstBAI YACGTR 1 cut(s) 949
BstC8I GCNNGC 2 cut(s) 1231, 1309
BstDEI CTNAG 5 cut(s) 98, 138, 287, 729, 1194
BstF5I GGATG 4 cut(s) 433, 534, 874, 1042
BstFNI CGCG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 4 cut(s) 88, 280, 778, 1404
BstMAI GTCTC 4 cut(s) 163, 291, 1015, 1259
BstMWI GCNNNNNNNGC 3 cut(s) 568, 1401, 1415
BstNI CCWGG 2 cut(s) 539, 1034
BstSCI CCNGG 3 cut(s) 263, 537, 1032
BstSFI CTRYAG 3 cut(s) 651, 979, 1227
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 4 cut(s) 123, 862, 1213, 1220
BstX2I RGATCY 4 cut(s) 35, 267, 1021, 1288
BstYI RGATCY 4 cut(s) 35, 267, 1021, 1288
BsuRI GGCC 2 cut(s) 677, 836
BtgZI GCGATG 1 cut(s) 1267
BtsCI GGATG 4 cut(s) 433, 534, 874, 1042
BtsIMutI CAGTG 2 cut(s) 843, 1191
BveI ACCTGC 1 cut(s) 488
Cac8I GCNNGC 2 cut(s) 1231, 1309
CaiI CAGNNNCTG 3 cut(s) 503, 578, 1193
CciI TCATGA 1 cut(s) 891
CfoI GCGC 4 cut(s) 88, 280, 778, 1404
Cfr10I RCCGGY 2 cut(s) 836, 1409
Cfr13I GGNCC 1 cut(s) 432
CseI GACGC 1 cut(s) 295
Csp6I GTAC 2 cut(s) 526, 950
CviAII CATG 7 cut(s) 93, 568, 608, 892, 1050, 1335, 1431
CviQI GTAC 2 cut(s) 526, 950
DdeI CTNAG 5 cut(s) 98, 138, 287, 729, 1194
DrdI GACNNNNNNGTC 2 cut(s) 305, 1103
DseDI GACNNNNNNGTC 2 cut(s) 305, 1103
Eam1104I CTCTTC 2 cut(s) 1112, 1377
EarI CTCTTC 2 cut(s) 1112, 1377
Ecl136II GAGCTC 2 cut(s) 944, 1047
Eco147I AGGCCT 1 cut(s) 677
Eco24I GRGCYC 2 cut(s) 946, 1049
Eco32I GATATC 1 cut(s) 1078
Eco47I GGWCC 1 cut(s) 432
Eco47III AGCGCT 1 cut(s) 279
Eco53kI GAGCTC 2 cut(s) 944, 1047
Eco57I CTGAAG 2 cut(s) 14, 1149
Eco88I CYCGRG 1 cut(s) 452
EcoICRI GAGCTC 2 cut(s) 944, 1047
EcoO109I RGGNCCY 1 cut(s) 432
EcoRI GAATTC 1 cut(s) 986
EcoRII CCWGG 2 cut(s) 537, 1032
EcoRV GATATC 1 cut(s) 1078
EcoT38I GRGCYC 2 cut(s) 946, 1049
FaeI CATG 7 cut(s) 96, 571, 611, 895, 1053, 1338, 1434
FalI AAGNNNNNCTT 2 cut(s) 1197, 1229
FaqI GGGAC 3 cut(s) 445, 816, 1198
FatI CATG 7 cut(s) 92, 567, 607, 891, 1049, 1334, 1430
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 4 cut(s) 112, 876, 1209, 1227
FokI GGATG 4 cut(s) 440, 521, 881, 1049
FriOI GRGCYC 2 cut(s) 946, 1049
Fsp4HI GCNGC 4 cut(s) 112, 876, 1209, 1227
FspBI CTAG 1 cut(s) 806
GlaI GCGC 4 cut(s) 87, 279, 777, 1403
GluI GCNGC 4 cut(s) 112, 876, 1209, 1227
GsaI CCCAGC 2 cut(s) 615, 1237
GsuI CTGGAG 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 2 cut(s) 677, 836
HapII CCGG 4 cut(s) 63, 264, 837, 1410
HgaI GACGC 1 cut(s) 295
HhaI GCGC 4 cut(s) 88, 280, 778, 1404
Hin1II CATG 7 cut(s) 96, 571, 611, 895, 1053, 1338, 1434
Hin6I GCGC 4 cut(s) 86, 278, 776, 1402
HinP1I GCGC 4 cut(s) 86, 278, 776, 1402
HincII GTYRAC 2 cut(s) 382, 1174
HindII GTYRAC 2 cut(s) 382, 1174
HindIII AAGCTT 1 cut(s) 1416
HinfI GANTC 6 cut(s) 80, 102, 193, 964, 1196, 1217
HpaI GTTAAC 1 cut(s) 1174
HpaII CCGG 4 cut(s) 63, 264, 837, 1410
HphI GGTGA 3 cut(s) 14, 1419, 1424
Hpy166II GTNNAC 3 cut(s) 382, 1174, 1346
Hpy188I TCNGA 8 cut(s) 33, 99, 198, 216, 673, 758, 883, 1424
Hpy188III TCNNGA 7 cut(s) 549, 682, 740, 788, 892, 922, 1165
Hpy8I GTNNAC 3 cut(s) 382, 1174, 1346
Hpy99I CGWCG 3 cut(s) 55, 111, 202
HpyAV CCTTC 4 cut(s) 688, 706, 758, 1162
HpyCH4III ACNGT 4 cut(s) 202, 954, 1146, 1189
HpyCH4IV ACGT 2 cut(s) 384, 948
HpyF10VI GCNNNNNNNGC 3 cut(s) 568, 1401, 1415
HpyF3I CTNAG 5 cut(s) 98, 138, 287, 729, 1194
HpySE526I ACGT 2 cut(s) 384, 948
Hsp92II CATG 7 cut(s) 96, 571, 611, 895, 1053, 1338, 1434
HspAI GCGC 4 cut(s) 86, 278, 776, 1402
KflI GGGWCCC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 591
KspAI GTTAAC 1 cut(s) 1174
LmnI GCTCC 7 cut(s) 367, 506, 559, 656, 941, 1044, 1336
Lsp1109I GCAGC 4 cut(s) 123, 862, 1213, 1220
LweI GCATC 5 cut(s) 266, 397, 733, 811, 887
MaeI CTAG 1 cut(s) 806
MaeII ACGT 2 cut(s) 384, 948
MaeIII GTNAC 4 cut(s) 839, 1105, 1189, 1250
MboII GAAGA 4 cut(s) 20, 1031, 1129, 1394
MfeI CAATTG 2 cut(s) 825, 1005
MflI RGATCY 4 cut(s) 35, 267, 1021, 1288
MhlI GDGCHC 3 cut(s) 946, 1049, 1333
MluCI AATT 5 cut(s) 825, 884, 986, 1005, 1200
MlyI GAGTC 3 cut(s) 973, 1205, 1211
MmeI TCCRAC 3 cut(s) 221, 736, 1105
MseI TTAA 3 cut(s) 339, 1137, 1173
MslI CAYNNNNRTG 1 cut(s) 1257
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 4 cut(s) 63, 264, 837, 1410
MspR9I CCNGG 3 cut(s) 265, 539, 1034
MunI CAATTG 2 cut(s) 825, 1005
Mva1269I GAATGC 1 cut(s) 581
MvaI CCWGG 2 cut(s) 539, 1034
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 3 cut(s) 568, 1401, 1415
NciI CCSGG 1 cut(s) 265
NlaIII CATG 7 cut(s) 96, 571, 611, 895, 1053, 1338, 1434
NlaIV GGNNCC 4 cut(s) 363, 392, 433, 434
NmeAIII GCCGAG 2 cut(s) 985, 1371
NmuCI GTSAC 3 cut(s) 1105, 1189, 1250
OliI CACNNNNGTG 1 cut(s) 1257
PagI TCATGA 1 cut(s) 891
PaqCI CACCTGC 1 cut(s) 488
PceI AGGCCT 1 cut(s) 677
PctI GAATGC 1 cut(s) 581
PfeI GAWTC 3 cut(s) 80, 102, 193
PflMI CCANNNNNTGG 2 cut(s) 938, 1087
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 4 cut(s) 113, 877, 1210, 1228
PleI GAGTC 3 cut(s) 972, 1204, 1211
PpsI GAGTC 3 cut(s) 972, 1204, 1211
Ppu21I YACGTR 1 cut(s) 949
PpuMI RGGWCCY 1 cut(s) 432
Psp124BI GAGCTC 2 cut(s) 946, 1049
Psp5II RGGWCCY 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 537, 1032
PspFI CCCAGC 2 cut(s) 611, 1233
PspGI CCWGG 2 cut(s) 537, 1032
PspN4I GGNNCC 4 cut(s) 363, 392, 433, 434
PspPI GGNCC 1 cut(s) 432
PspPPI RGGWCCY 1 cut(s) 432
PstI CTGCAG 1 cut(s) 1231
PstNI CAGNNNCTG 3 cut(s) 503, 578, 1193
PsuI RGATCY 4 cut(s) 35, 267, 1021, 1288
RsaI GTAC 2 cut(s) 527, 951
RsaNI GTAC 2 cut(s) 526, 950
RseI CAYNNNNRTG 1 cut(s) 1257
SacI GAGCTC 2 cut(s) 946, 1049
SaqAI TTAA 3 cut(s) 339, 1137, 1173
SatI GCNGC 4 cut(s) 112, 876, 1209, 1227
Sau96I GGNCC 1 cut(s) 432
SchI GAGTC 3 cut(s) 973, 1205, 1211
ScrFI CCNGG 3 cut(s) 265, 539, 1034
SduI GDGCHC 3 cut(s) 946, 1049, 1333
SfaNI GCATC 5 cut(s) 266, 397, 733, 811, 887
SfcI CTRYAG 3 cut(s) 651, 979, 1227
SgrAI CRCCGGYG 1 cut(s) 1409
SinI GGWCC 1 cut(s) 432
SmiMI CAYNNNNRTG 1 cut(s) 1257
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SpeI ACTAGT 1 cut(s) 805
Sse9I AATT 5 cut(s) 825, 884, 986, 1005, 1200
SseBI AGGCCT 1 cut(s) 677
SsiI CCGC 2 cut(s) 114, 1311
SspMI CTAG 1 cut(s) 806
SstI GAGCTC 2 cut(s) 946, 1049
StuI AGGCCT 1 cut(s) 677
StyD4I CCNGG 3 cut(s) 263, 537, 1032
TaaI ACNGT 4 cut(s) 202, 954, 1146, 1189
TaiI ACGT 2 cut(s) 387, 951
TaqI TCGA 4 cut(s) 405, 446, 1017, 1380
TasI AATT 5 cut(s) 825, 884, 986, 1005, 1200
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 3 cut(s) 80, 102, 193
Tru1I TTAA 3 cut(s) 339, 1137, 1173
Tru9I TTAA 3 cut(s) 339, 1137, 1173
TscAI CASTG 2 cut(s) 850, 1198
TseFI GTSAC 3 cut(s) 1105, 1189, 1250
TseI GCWGC 4 cut(s) 111, 875, 1208, 1226
Tsp45I GTSAC 3 cut(s) 1105, 1189, 1250
TspDTI ATGAA 7 cut(s) 366, 777, 880, 908, 979, 1047, 1270
TspGWI ACGGA 3 cut(s) 39, 95, 1364
TspRI CASTG 2 cut(s) 850, 1198
Van91I CCANNNNNTGG 2 cut(s) 938, 1087
VpaK11BI GGWCC 1 cut(s) 432
XapI RAATTY 2 cut(s) 884, 986
XspI CTAG 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.