Rorug06G0463700

Enolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012756
Physical Location & Seq
Forward (+)
58882121 .. 58882498
378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0463700.1

Sequence Viewer

Length: 378 bp
ATGATCAACATCAGGTCATTCGCCTCTTCCCAGAAGCCCAATGAGCCGGTGGCCTCACGCGACCGGACCAGCACTGCCAAGGTCTTGACAACTGGAGCAATCGGTGTCACTTTCTTAGTCTTGTCCGGGTTAACTTTAACCGGGACAGTGATGACCCTGATCTTGGCCAGCCCAGTTATAGTGCTGTTTAGTCCGGTCCTAGTCCCGGCTGGGATAGTCCTCTTCCTTGCAGCAAGTGGTTTGGTTTTCTCCGGCGGGTGTGGCGTGGTGGCGATTATGGCACTATCATGGTTGATTAAATACGCATCTGACTACCTCGCTGCCAAGAAAAGGGCTGACGCATATGGGGGCTCTAATTTCATGCAAATTGGTCTATGA

Protein Analysis

125

Amino Acids

12.94

Weight (kDa)

9.8

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 26 - 104 1.5e-27 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012756)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74030
fragaria_vesca FvH4_5g12990 FvH4_5g12990 FvH4_5g12990
malus_domestica MD06G1208300.v1.1 MD14G1218700.v1.1
prunus_persica Prupe.5G212800_v2.0.a1
pyrus_communis pycom06g18610
rosa_chinensis RchiOBHm_Chr7g0184181
rosa_laevigata RLG00000004996
rosa_multiflora Rmu_sc0012221.1_g000006
rosa_roxburghii Rroxscaffold_3G00269820
rosa_rugosa Rorug06G0463700
rosa_samantha Rh7AG082200 Rh7BG065000 Rh7CG065400 Rh7DG065100
rosa_wichuraiana Rw7G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 60
AciI CCGC 1 cut(s) 255
AcoI YGGCCR 1 cut(s) 165
AfiI CCNNNNNNNGG 3 cut(s) 163, 205, 330
AoxI GGCC 2 cut(s) 51, 165
ApeKI GCWGC 2 cut(s) 230, 320
AspS9I GGNCC 2 cut(s) 66, 196
AsuC2I CCSGG 3 cut(s) 127, 142, 206
AvaII GGWCC 2 cut(s) 66, 196
BalI TGGCCA 1 cut(s) 167
BanII GRGCYC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 242, 307
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 3 cut(s) 127, 142, 206
BfaI CTAG 1 cut(s) 200
BisI GCNGC 2 cut(s) 231, 321
BlsI GCNGC 2 cut(s) 232, 322
Bme1390I CCNGG 3 cut(s) 127, 142, 206
Bme18I GGWCC 2 cut(s) 66, 196
BmgT120I GGNCC 2 cut(s) 66, 196
BmrFI CCNGG 3 cut(s) 127, 142, 206
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 314
BmuI ACTGGG 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 114
BpuMI CCSGG 3 cut(s) 127, 142, 206
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 2 cut(s) 63, 193
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 205, 330
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 2 cut(s) 97, 173
Bse8I GATNNNNATC 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 3 cut(s) 163, 205, 330
BseNI ACTGG 2 cut(s) 97, 173
BseXI GCAGC 2 cut(s) 242, 307
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 53, 167
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 7 cut(s) 47, 64, 126, 141, 194, 206, 252
BslFI GGGAC 2 cut(s) 157, 188
BslI CCNNNNNNNGG 3 cut(s) 163, 205, 330
BsmFI GGGAC 2 cut(s) 157, 188
BsnI GGCC 2 cut(s) 53, 167
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 2 cut(s) 3, 159
BspACI CCGC 1 cut(s) 255
BspANI GGCC 2 cut(s) 53, 167
BspFNI CGCG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 2 cut(s) 97, 173
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 2 cut(s) 3, 159
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 2 cut(s) 31, 227
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 1 cut(s) 115
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 2 cut(s) 6, 162
BstMBI GATC 2 cut(s) 3, 159
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 3 cut(s) 43, 261, 278
BstSCI CCNGG 3 cut(s) 125, 140, 204
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 242, 307
BsuRI GGCC 2 cut(s) 53, 167
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 72, 153
Cac8I GCNNGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 2 cut(s) 66, 196
CseI GACGC 1 cut(s) 347
CviAII CATG 2 cut(s) 288, 361
CviJI RGCY 8 cut(s) 37, 46, 53, 167, 171, 209, 335, 351
CviKI_1 RGCY 8 cut(s) 37, 46, 53, 167, 171, 209, 335, 351
DdeI CTNAG 1 cut(s) 115
DpnI GATC 2 cut(s) 5, 161
DpnII GATC 2 cut(s) 3, 159
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 2 cut(s) 31, 227
EarI CTCTTC 2 cut(s) 31, 227
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 66, 196
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 2 cut(s) 291, 364
FaiI YATR 7 cut(s) 179, 278, 289, 343, 345, 362, 376
FaqI GGGAC 2 cut(s) 157, 188
FatI CATG 2 cut(s) 287, 360
FauI CCCGC 1 cut(s) 248
FauNDI CATATG 1 cut(s) 343
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 231, 321
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 231, 321
FspBI CTAG 1 cut(s) 200
GluI GCNGC 2 cut(s) 231, 321
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 2 cut(s) 53, 167
HapII CCGG 7 cut(s) 47, 64, 126, 141, 194, 206, 252
HgaI GACGC 1 cut(s) 347
Hin1II CATG 2 cut(s) 291, 364
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HpaI GTTAAC 1 cut(s) 132
HpaII CCGG 7 cut(s) 47, 64, 126, 141, 194, 206, 252
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 1 cut(s) 85
Hpy8I GTNNAC 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 230, 364
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 261, 278
HpyF3I CTNAG 1 cut(s) 115
Hsp92II CATG 2 cut(s) 291, 364
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 132
Kzo9I GATC 2 cut(s) 3, 159
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 2 cut(s) 242, 307
LweI GCATC 1 cut(s) 314
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 5, 161
MboI GATC 2 cut(s) 3, 159
MboII GAAGA 2 cut(s) 18, 214
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 2 cut(s) 355, 366
MluNI TGGCCA 1 cut(s) 167
MnlI CCTC 4 cut(s) 34, 64, 230, 326
Mox20I TGGCCA 1 cut(s) 167
MscI TGGCCA 1 cut(s) 167
MseI TTAA 3 cut(s) 131, 137, 297
MslI CAYNNNNRTG 1 cut(s) 286
Msp20I TGGCCA 1 cut(s) 167
MspI CCGG 7 cut(s) 47, 64, 126, 141, 194, 206, 252
MspR9I CCNGG 3 cut(s) 127, 142, 206
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 43, 261, 278
NciI CCSGG 3 cut(s) 127, 142, 206
NdeI CATATG 1 cut(s) 343
NdeII GATC 2 cut(s) 3, 159
NlaIII CATG 2 cut(s) 291, 364
NmuCI GTSAC 1 cut(s) 106
PkrI GCNGC 2 cut(s) 232, 322
PspFI CCCAGC 1 cut(s) 209
PspPI GGNCC 2 cut(s) 66, 196
RseI CAYNNNNRTG 1 cut(s) 286
SaqAI TTAA 3 cut(s) 131, 137, 297
SatI GCNGC 2 cut(s) 231, 321
Sau3AI GATC 2 cut(s) 3, 159
Sau96I GGNCC 2 cut(s) 66, 196
ScrFI CCNGG 3 cut(s) 127, 142, 206
SduI GDGCHC 1 cut(s) 353
SetI ASST 3 cut(s) 17, 84, 318
SfaNI GCATC 1 cut(s) 314
SinI GGWCC 2 cut(s) 66, 196
SmiMI CAYNNNNRTG 1 cut(s) 286
Sse9I AATT 2 cut(s) 355, 366
SsiI CCGC 1 cut(s) 255
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 3 cut(s) 125, 140, 204
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 148
TasI AATT 2 cut(s) 355, 366
Tru1I TTAA 3 cut(s) 131, 137, 297
Tru9I TTAA 3 cut(s) 131, 137, 297
TscAI CASTG 2 cut(s) 79, 153
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 230, 320
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 349
TspRI CASTG 2 cut(s) 79, 153
VpaK11BI GGWCC 2 cut(s) 66, 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.