Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0012756

Physical Location & Seq

Genomic Coordinates Reverse (-)

34223843 .. 34226476

Gene Structure 2634 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1208300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGGCTCTAGCTACCCAGCCCACCACTAACCTCCTCCAAAACCCCTTCCTCTCCTCCAAACCCCAACCCAGACCCCAGCAGCTCCCCACGGCCGTCCCCTGCCCGGCCCGGCGTCGTTCAGGCTCCGTCCAATGCTCCGTCGCCGTCGCGCCCGCCGCGGCCTCCAAGGTCTCCAAGGAGTACAAGGTCAAGTCGGTGAAGGCGAGGCAGATCATTGATAGCAGGGGCAATCCGACCGTCGAGGTTGATCTGGTCACCGACGATCTGTATCGATCGGCCGTGCCCAGTGGCGCCTCTACCGGAATCTACGAGGCGTTGGAGCTCAGGGACGGTGACAAGGCGGTCTACGGCGGGAAAGGTGTGCTCACTGCTGTGAAGAATATCAATGAGATTTTGGGACCCAAGCTCATTGGCGTCGATGTCAGAAATCAAAATGAGGTCGATGCACTTATGCTGGAAATCGATGGAACCCCAAACAAGTCGAAACTGGGGGCCAATGCCATTTTGGGAGTATCTCTGAGCGTGTGTAGAGCGGGAGCAGGAGCAAAGGGAGTGCCATTGTACAAGCACATCCAGGAAGTGTCGGGAATAAAGGAGCTCGTTATGCCTGTCCCGGCTTTCAATGTGATCAATGGAGGCAGCCATGCTGGAAATAATCTGGCAATGCAGGAGTTTATGATCCTGCCTGTGGGTGCATCCTCGTTCGCTGAGGCACTCCGCATGGGTAGTGAAGTTTACCATACACTAAAGGGAATTATCAAGGCAAAATATGGACAGGATGCTTGCAATGTTGGAGATGAAGGGGGGTTTGCTCCCAATGTGCAGGATAACAGGGAGGGACTGGTTTTGCTCATTGATGCTATCGAAAAGGCTGGTTACACCGGAAAGATCAAAATCGGAATGGATGTTGCAGCCTCGGAATTTCTCACTAAAGAAGGGAAATACGATCTCAACTTTAAGAAACAACCAAATGACGGAGCCCATGTGCTCTCTCCTCCGAACCTTGGCGAGCTGTACAAGGAGTTCATTAAGGAATTCCCGATTGTGTCCATTGAAGATCCATTCGACCAAGACGATTGGAGCTCGTGGTCTTCACTTCAGTCTTCTGTCGATATCCAACTCGTCGGAGATGACTTGTTGGTCACAAATCCAACAAAAATTGCTGAAGCCATTCAGAAGAAGGCATGCAATGGCTTACTTCTAAAGGCGAGCATATTTGATTGCAGTTGTTTTCCAATAAATGGTATCAACCTTGTCAACCAGATTGGCACAATAACTGAATCTATTCAGGCAGCACTCGATGCGAAAGCTGCAGGTTGGGGTGTCATGGTCAGCCACCGGAGTGGAGAAACTGAAGATAACTTTATCGCTGATCTTTCCGTTGGCTTGGCCAGTGGACAGATCAAGACCGGAGCTCCCTGCCGGAGTGAGCGGTTGGCCAAGTATAATCAGCTTCTGCGTATTGAAGAGGAACTCGGCAATGTGCGTTATGCTGGCGAAGCTTTCAGATCTCCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004634 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010090 GO:0016043 GO:0016829 GO:0016835 GO:0016836 GO:0030154 GO:0032502 GO:0032989 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0071840 GO:0090558 GO:0090626

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

506

Amino Acids

53.91

Weight (kDa)

5.76

Isoelectric Point (pI)

38.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	63 - 191	1.1e-55	Enolase, N-terminal domain
Enolase_C	PF00113	200 - 503	4.9e-139	Enolase, C-terminal TIM barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74030
fragaria_vesca	FvH4_5g12990 FvH4_5g12990 FvH4_5g12990
malus_domestica	MD06G1208300.v1.1 MD14G1218700.v1.1
prunus_persica	Prupe.5G212800_v2.0.a1
pyrus_communis	pycom06g18610
rosa_chinensis	RchiOBHm_Chr7g0184181
rosa_laevigata	RLG00000004996
rosa_multiflora	Rmu_sc0012221.1_g000006
rosa_roxburghii	Rroxscaffold_3G00269820
rosa_rugosa	Rorug06G0463700
rosa_samantha	Rh7AG082200 Rh7BG065000 Rh7CG065400 Rh7DG065100
rosa_wichuraiana	Rw7G005330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	341, 1139
Acc36I	ACCTGC	1 cut(s)	1304
AccB1I	GGYRCC	1 cut(s)	290
AccB7I	CCANNNNNTGG	1 cut(s)	1241
AccBSI	CCGCTC	2 cut(s)	533, 1432
AccI	GTMKAC	1 cut(s)	345
AccII	CGCG	2 cut(s)	149, 158
AciI	CCGC	8 cut(s)	153, 156, 158, 341, 351, 533, 718, 1432
AclWI	GGATC	2 cut(s)	673, 1052
AcoI	YGGCCR	4 cut(s)	90, 276, 1389, 1437
AcsI	RAATTY	2 cut(s)	920, 1034
AcuI	CTGAAG	3 cut(s)	1082, 1185, 1374
AcyI	GRCGYC	3 cut(s)	112, 291, 414
AfaI	GTAC	3 cut(s)	182, 563, 1016
AfiI	CCNNNNNNNGG	5 cut(s)	103, 688, 974, 1004, 1241
AgsI	TTSAA	3 cut(s)	622, 1055, 1466
AjnI	CCWGG	1 cut(s)	573
AleI	CACNNNNGTG	2 cut(s)	371, 1341
Alw21I	GWGCWC	6 cut(s)	324, 366, 600, 990, 1085, 1417
Alw26I	GTCTC	1 cut(s)	175
AlwI	GGATC	2 cut(s)	673, 1052
AlwNI	CAGNNNCTG	1 cut(s)	1456
AoxI	GGCC	7 cut(s)	90, 105, 159, 276, 492, 1389, 1437
ApeKI	GCWGC	5 cut(s)	79, 639, 911, 1292, 1310
ApoI	RAATTY	2 cut(s)	920, 1034
Asp700I	GAANNNNTTC	2 cut(s)	1170, 1284
AspLEI	GCGC	2 cut(s)	151, 293
AspS9I	GGNCC	3 cut(s)	106, 398, 492
AsuC2I	CCSGG	3 cut(s)	104, 109, 614
AsuHPI	GGTGA	3 cut(s)	208, 247, 344
AvaII	GGWCC	1 cut(s)	398
BaeGI	GKGCMC	1 cut(s)	285
BalI	TGGCCA	2 cut(s)	1391, 1439
BanI	GGYRCC	1 cut(s)	290
BanII	GRGCYC	5 cut(s)	324, 600, 982, 1085, 1417
BauI	CACGAG	1 cut(s)	1084
BbsI	GAAGAC	2 cut(s)	1083, 1095
Bbv12I	GWGCWC	6 cut(s)	324, 366, 600, 990, 1085, 1417
BbvCI	CCTCAGC	1 cut(s)	708
BbvI	GCAGC	5 cut(s)	91, 651, 923, 1297, 1304
BccI	CCATC	1 cut(s)	458
BceAI	ACGGC	5 cut(s)	77, 105, 128, 263, 364
BciT130I	CCWGG	1 cut(s)	575
BclI	TGATCA	1 cut(s)	627
BcnI	CCSGG	3 cut(s)	104, 109, 614
BcoDI	GTCTC	1 cut(s)	175
BfaI	CTAG	1 cut(s)	8
BfmI	CTRYAG	1 cut(s)	1311
BfoI	RGCGCY	1 cut(s)	294
BfuAI	ACCTGC	1 cut(s)	1304
BglII	AGATCT	1 cut(s)	1508
BisI	GCNGC	7 cut(s)	80, 156, 159, 640, 912, 1293, 1311
BlsI	GCNGC	7 cut(s)	81, 157, 160, 641, 913, 1294, 1312
Bme1390I	CCNGG	4 cut(s)	104, 109, 575, 614
Bme18I	GGWCC	1 cut(s)	398
BmgT120I	GGNCC	3 cut(s)	106, 398, 492
BmiI	GGNNCC	7 cut(s)	124, 292, 399, 400, 469, 493, 979
BmrFI	CCNGG	4 cut(s)	104, 109, 575, 614
BmrI	ACTGGG	2 cut(s)	279, 497
BmsI	GCATC	5 cut(s)	433, 704, 769, 847, 1291
BmuI	ACTGGG	2 cut(s)	279, 497
BpiI	GAAGAC	2 cut(s)	1083, 1095
Bpu10I	CCTNAGC	2 cut(s)	323, 708
BpuMI	CCSGG	3 cut(s)	104, 109, 614
Bsa29I	ATCGAT	2 cut(s)	271, 462
BsaBI	GATNNNNATC	2 cut(s)	267, 893
BsaHI	GRCGYC	3 cut(s)	112, 291, 414
BsaI	GGTCTC	1 cut(s)	175
BsaJI	CCNNGG	6 cut(s)	87, 156, 165, 174, 915, 1003
BsaWI	WCCGGW	4 cut(s)	299, 881, 1338, 1409
BsaXI	ACNNNNNCTCC	4 cut(s)	969, 999, 1399, 1429
Bsc4I	CCNNNNNNNGG	5 cut(s)	103, 688, 974, 1004, 1241
Bse1I	ACTGG	4 cut(s)	285, 492, 846, 1392
Bse3DI	GCAATG	4 cut(s)	669, 793, 1195, 1486
Bse8I	GATNNNNATC	2 cut(s)	267, 893
BseBI	CCWGG	1 cut(s)	575
BseCI	ATCGAT	2 cut(s)	271, 462
BseDI	CCNNGG	6 cut(s)	87, 156, 165, 174, 915, 1003
BseGI	GGATG	4 cut(s)	570, 695, 784, 910
BseJI	GATNNNNATC	2 cut(s)	267, 893
BseLI	CCNNNNNNNGG	5 cut(s)	103, 688, 974, 1004, 1241
BseMI	GCAATG	4 cut(s)	669, 793, 1195, 1486
BseMII	CTCAG	3 cut(s)	337, 509, 699
BseNI	ACTGG	4 cut(s)	285, 492, 846, 1392
BseRI	GAGGAG	3 cut(s)	23, 43, 984
BseSI	GKGCMC	1 cut(s)	285
BseX3I	CGGCCG	2 cut(s)	90, 276
BseXI	GCAGC	5 cut(s)	91, 651, 923, 1297, 1304
BseYI	CCCAGC	2 cut(s)	15, 75
BsgI	GTGCAG	1 cut(s)	842
Bsh1236I	CGCG	2 cut(s)	149, 158
Bsh1285I	CGRYCG	4 cut(s)	93, 237, 275, 279
BshFI	GGCC	7 cut(s)	92, 107, 161, 278, 494, 1391, 1439
BshNI	GGYRCC	1 cut(s)	290
BshVI	ATCGAT	2 cut(s)	271, 462
BsiEI	CGRYCG	4 cut(s)	93, 237, 275, 279
BsiHKAI	GWGCWC	6 cut(s)	324, 366, 600, 990, 1085, 1417
BsiSI	CCGG	8 cut(s)	104, 109, 300, 614, 882, 1339, 1410, 1423
BslFI	GGGAC	5 cut(s)	80, 341, 411, 596, 852
BslI	CCNNNNNNNGG	5 cut(s)	103, 688, 974, 1004, 1241
BsmAI	GTCTC	1 cut(s)	175
BsmFI	GGGAC	5 cut(s)	80, 341, 411, 596, 852
BsnI	GGCC	7 cut(s)	92, 107, 161, 278, 494, 1391, 1439
Bso31I	GGTCTC	1 cut(s)	175
Bsp1286I	GDGCHC	8 cut(s)	285, 324, 366, 600, 982, 990, 1085, 1417
Bsp1407I	TGTACA	2 cut(s)	561, 1014
BspACI	CCGC	8 cut(s)	153, 156, 158, 341, 351, 533, 718, 1432
BspANI	GGCC	7 cut(s)	92, 107, 161, 278, 494, 1391, 1439
BspCNI	CTCAG	3 cut(s)	336, 510, 700
BspDI	ATCGAT	2 cut(s)	271, 462
BspFNI	CGCG	2 cut(s)	149, 158
BspLI	GGNNCC	7 cut(s)	124, 292, 399, 400, 469, 493, 979
BspMAI	CTGCAG	1 cut(s)	1315
BspMI	ACCTGC	1 cut(s)	1304
BspPI	GGATC	2 cut(s)	673, 1052
BspT107I	GGYRCC	1 cut(s)	290
BspTNI	GGTCTC	1 cut(s)	175
BsrBI	CCGCTC	2 cut(s)	533, 1432
BsrDI	GCAATG	4 cut(s)	669, 793, 1195, 1486
BsrGI	TGTACA	2 cut(s)	561, 1014
BsrI	ACTGG	4 cut(s)	285, 492, 846, 1392
BssECI	CCNNGG	6 cut(s)	87, 156, 165, 174, 915, 1003
BssNI	GRCGYC	3 cut(s)	112, 291, 414
BssSI	CACGAG	1 cut(s)	1084
BssT1I	CCWWGG	3 cut(s)	165, 174, 1003
Bst2BI	CACGAG	1 cut(s)	1084
Bst2UI	CCWGG	1 cut(s)	575
Bst4CI	ACNGT	2 cut(s)	238, 332
Bst6I	CTCTTC	1 cut(s)	1461
BstACI	GRCGYC	3 cut(s)	112, 291, 414
BstAPI	GCANNNNNTGC	1 cut(s)	1301
BstAUI	TGTACA	2 cut(s)	561, 1014
BstC8I	GCNNGC	6 cut(s)	153, 784, 1010, 1186, 1210, 1495
BstDEI	CTNAG	3 cut(s)	323, 518, 708
BstDSI	CCRYGG	2 cut(s)	87, 156
BstEII	GGTNACC	1 cut(s)	253
BstF5I	GGATG	4 cut(s)	570, 695, 784, 910
BstFNI	CGCG	2 cut(s)	149, 158
BstH2I	RGCGCY	1 cut(s)	294
BstHHI	GCGC	2 cut(s)	151, 293
BstMAI	GTCTC	1 cut(s)	175
BstMCI	CGRYCG	4 cut(s)	93, 237, 275, 279
BstMWI	GCNNNNNNNGC	5 cut(s)	155, 604, 1301, 1310, 1499
BstNI	CCWGG	1 cut(s)	575
BstNSI	RCATGY	1 cut(s)	1188
BstPI	GGTNACC	1 cut(s)	253
BstSCI	CCNGG	4 cut(s)	102, 107, 573, 612
BstSFI	CTRYAG	1 cut(s)	1311
BstSLI	GKGCMC	1 cut(s)	285
BstUI	CGCG	2 cut(s)	149, 158
BstV1I	GCAGC	5 cut(s)	91, 651, 923, 1297, 1304
BstV2I	GAAGAC	2 cut(s)	1083, 1095
BstX2I	RGATCY	2 cut(s)	1057, 1508
BstXI	CCANNNNNNTGG	1 cut(s)	1343
BstYI	RGATCY	2 cut(s)	1057, 1508
BstZI	CGGCCG	2 cut(s)	90, 276
Bsu15I	ATCGAT	2 cut(s)	271, 462
BsuRI	GGCC	7 cut(s)	92, 107, 161, 278, 494, 1391, 1439
BsuTUI	ATCGAT	2 cut(s)	271, 462
BtgI	CCRYGG	2 cut(s)	87, 156
BtsCI	GGATG	4 cut(s)	570, 695, 784, 910
BtsI	GCAGTG	1 cut(s)	366
BtsIMutI	CAGTG	3 cut(s)	292, 366, 1399
BveI	ACCTGC	1 cut(s)	1304
Cac8I	GCNNGC	6 cut(s)	153, 784, 1010, 1186, 1210, 1495
CaiI	CAGNNNCTG	1 cut(s)	1456
CfoI	GCGC	2 cut(s)	151, 293
Cfr13I	GGNCC	3 cut(s)	106, 398, 492
Cfr42I	CCGCGG	1 cut(s)	159
ClaI	ATCGAT	2 cut(s)	271, 462
CseI	GACGC	2 cut(s)	101, 403
Csp6I	GTAC	3 cut(s)	181, 562, 1015
CviAII	CATG	5 cut(s)	644, 721, 983, 1185, 1327
CviQI	GTAC	3 cut(s)	181, 562, 1015
DdeI	CTNAG	3 cut(s)	323, 518, 708
DinI	GGCGCC	1 cut(s)	292
DrdI	GACNNNNNNGTC	2 cut(s)	341, 1139
DseDI	GACNNNNNNGTC	2 cut(s)	341, 1139
EaeI	YGGCCR	4 cut(s)	90, 276, 1389, 1437
EagI	CGGCCG	2 cut(s)	90, 276
Eam1104I	CTCTTC	1 cut(s)	1461
EarI	CTCTTC	1 cut(s)	1461
Ecl136II	GAGCTC	4 cut(s)	322, 598, 1083, 1415
EclXI	CGGCCG	2 cut(s)	90, 276
Eco130I	CCWWGG	3 cut(s)	165, 174, 1003
Eco24I	GRGCYC	5 cut(s)	324, 600, 982, 1085, 1417
Eco31I	GGTCTC	1 cut(s)	175
Eco32I	GATATC	1 cut(s)	1114
Eco47I	GGWCC	1 cut(s)	398
Eco52I	CGGCCG	2 cut(s)	90, 276
Eco53kI	GAGCTC	4 cut(s)	322, 598, 1083, 1415
Eco57I	CTGAAG	3 cut(s)	1082, 1185, 1374
Eco91I	GGTNACC	1 cut(s)	253
EcoICRI	GAGCTC	4 cut(s)	322, 598, 1083, 1415
EcoO109I	RGGNCCY	1 cut(s)	398
EcoO65I	GGTNACC	1 cut(s)	253
EcoRI	GAATTC	1 cut(s)	1034
EcoRII	CCWGG	1 cut(s)	573
EcoRV	GATATC	1 cut(s)	1114
EcoT14I	CCWWGG	3 cut(s)	165, 174, 1003
EcoT38I	GRGCYC	5 cut(s)	324, 600, 982, 1085, 1417
EgeI	GGCGCC	1 cut(s)	292
EheI	GGCGCC	1 cut(s)	292
ErhI	CCWWGG	3 cut(s)	165, 174, 1003
FaeI	CATG	5 cut(s)	647, 724, 986, 1188, 1330
FaqI	GGGAC	5 cut(s)	80, 341, 411, 596, 852
FatI	CATG	5 cut(s)	643, 720, 982, 1184, 1326
FauI	CCCGC	3 cut(s)	160, 344, 526
FbaI	TGATCA	1 cut(s)	627
FblI	GTMKAC	1 cut(s)	345
Fnu4HI	GCNGC	7 cut(s)	80, 156, 159, 640, 912, 1293, 1311
FokI	GGATG	4 cut(s)	557, 682, 791, 917
FriOI	GRGCYC	5 cut(s)	324, 600, 982, 1085, 1417
Fsp4HI	GCNGC	7 cut(s)	80, 156, 159, 640, 912, 1293, 1311
FspBI	CTAG	1 cut(s)	8
GlaI	GCGC	2 cut(s)	150, 292
GluI	GCNGC	7 cut(s)	80, 156, 159, 640, 912, 1293, 1311
GsaI	CCCAGC	2 cut(s)	19, 79
HaeII	RGCGCY	1 cut(s)	294
HaeIII	GGCC	7 cut(s)	92, 107, 161, 278, 494, 1391, 1439
HapII	CCGG	8 cut(s)	104, 109, 300, 614, 882, 1339, 1410, 1423
HgaI	GACGC	2 cut(s)	101, 403
HhaI	GCGC	2 cut(s)	151, 293
Hin1I	GRCGYC	3 cut(s)	112, 291, 414
Hin1II	CATG	5 cut(s)	647, 724, 986, 1188, 1330
Hin6I	GCGC	2 cut(s)	149, 291
HinP1I	GCGC	2 cut(s)	149, 291
HincII	GTYRAC	1 cut(s)	1258
HindII	GTYRAC	1 cut(s)	1258
HindIII	AAGCTT	1 cut(s)	1500
HinfI	GANTC	2 cut(s)	303, 1280
HpaII	CCGG	8 cut(s)	104, 109, 300, 614, 882, 1339, 1410, 1423
HphI	GGTGA	3 cut(s)	208, 247, 344
Hpy166II	GTNNAC	4 cut(s)	346, 736, 1258, 1397
Hpy188I	TCNGA	9 cut(s)	234, 425, 519, 899, 919, 999, 1127, 1176, 1508
Hpy188III	TCNNGA	3 cut(s)	585, 1039, 1405
Hpy8I	GTNNAC	4 cut(s)	346, 736, 1258, 1397
Hpy99I	CGWCG	7 cut(s)	117, 143, 149, 242, 263, 419, 1127
HpyAV	CCTTC	5 cut(s)	55, 193, 794, 929, 1174
HpyCH4III	ACNGT	2 cut(s)	238, 332
HpyCH4V	TGCA	9 cut(s)	446, 667, 695, 786, 823, 911, 1188, 1224, 1313
HpyF10VI	GCNNNNNNNGC	5 cut(s)	155, 604, 1301, 1310, 1499
HpyF3I	CTNAG	3 cut(s)	323, 518, 708
Hsp92I	GRCGYC	3 cut(s)	112, 291, 414
Hsp92II	CATG	5 cut(s)	647, 724, 986, 1188, 1330
HspAI	GCGC	2 cut(s)	149, 291
KasI	GGCGCC	1 cut(s)	290
KflI	GGGWCCC	1 cut(s)	398
Ksp22I	TGATCA	1 cut(s)	627
KspI	CCGCGG	1 cut(s)	159
Lsp1109I	GCAGC	5 cut(s)	91, 651, 923, 1297, 1304
LweI	GCATC	5 cut(s)	433, 704, 769, 847, 1291
MaeI	CTAG	1 cut(s)	8
MaeIII	GTNAC	4 cut(s)	253, 332, 875, 1141
MbiI	CCGCTC	2 cut(s)	533, 1432
MboII	GAAGA	7 cut(s)	388, 1067, 1083, 1095, 1189, 1367, 1478
MflI	RGATCY	2 cut(s)	1057, 1508
MhlI	GDGCHC	8 cut(s)	285, 324, 366, 600, 982, 990, 1085, 1417
MlsI	TGGCCA	2 cut(s)	1391, 1439
MluCI	AATT	4 cut(s)	753, 920, 1034, 1158
MluNI	TGGCCA	2 cut(s)	1391, 1439
Mly113I	GGCGCC	1 cut(s)	291
MmeI	TCCRAC	6 cut(s)	257, 297, 772, 1105, 1141, 1175
Mox20I	TGGCCA	2 cut(s)	1391, 1439
MroXI	GAANNNNTTC	2 cut(s)	1170, 1284
MscI	TGGCCA	2 cut(s)	1391, 1439
MseI	TTAA	2 cut(s)	957, 1029
MslI	CAYNNNNRTG	2 cut(s)	371, 1341
Msp20I	TGGCCA	2 cut(s)	1391, 1439
MspA1I	CMGCKG	1 cut(s)	158
MspI	CCGG	8 cut(s)	104, 109, 300, 614, 882, 1339, 1410, 1423
MspR9I	CCNGG	4 cut(s)	104, 109, 575, 614
MvaI	CCWGG	1 cut(s)	575
MvnI	CGCG	2 cut(s)	149, 158
MwoI	GCNNNNNNNGC	5 cut(s)	155, 604, 1301, 1310, 1499
NarI	GGCGCC	1 cut(s)	291
NciI	CCSGG	3 cut(s)	104, 109, 614
NlaIII	CATG	5 cut(s)	647, 724, 986, 1188, 1330
NlaIV	GGNNCC	7 cut(s)	124, 292, 399, 400, 469, 493, 979
NmeAIII	GCCGAG	1 cut(s)	1455
NmuCI	GTSAC	3 cut(s)	253, 332, 1141
NspI	RCATGY	1 cut(s)	1188
OliI	CACNNNNGTG	2 cut(s)	371, 1341
PaeI	GCATGC	1 cut(s)	1188
PcsI	WCGNNNNNNNCGW	2 cut(s)	200, 1071
PdmI	GAANNNNTTC	2 cut(s)	1170, 1284
PfeI	GAWTC	2 cut(s)	303, 1280
PflMI	CCANNNNNTGG	1 cut(s)	1241
PfoI	TCCNGGA	1 cut(s)	573
PkrI	GCNGC	7 cut(s)	81, 157, 160, 641, 913, 1294, 1312
Ple19I	CGATCG	1 cut(s)	275
PluTI	GGCGCC	1 cut(s)	294
PpuMI	RGGWCCY	1 cut(s)	398
Psp124BI	GAGCTC	4 cut(s)	324, 600, 1085, 1417
Psp5II	RGGWCCY	1 cut(s)	398
Psp6I	CCWGG	1 cut(s)	573
PspEI	GGTNACC	1 cut(s)	253
PspFI	CCCAGC	2 cut(s)	15, 75
PspGI	CCWGG	1 cut(s)	573
PspN4I	GGNNCC	7 cut(s)	124, 292, 399, 400, 469, 493, 979
PspPI	GGNCC	3 cut(s)	106, 398, 492
PspPPI	RGGWCCY	1 cut(s)	398
PsrI	GAACNNNNNNTAC	2 cut(s)	1007, 1039
PstI	CTGCAG	1 cut(s)	1315
PstNI	CAGNNNCTG	1 cut(s)	1456
PsuI	RGATCY	2 cut(s)	1057, 1508
PvuI	CGATCG	1 cut(s)	275
RsaI	GTAC	3 cut(s)	182, 563, 1016
RsaNI	GTAC	3 cut(s)	181, 562, 1015
RseI	CAYNNNNRTG	2 cut(s)	371, 1341
SacI	GAGCTC	4 cut(s)	324, 600, 1085, 1417
SacII	CCGCGG	1 cut(s)	159
SaqAI	TTAA	2 cut(s)	957, 1029
SatI	GCNGC	7 cut(s)	80, 156, 159, 640, 912, 1293, 1311
Sau96I	GGNCC	3 cut(s)	106, 398, 492
ScrFI	CCNGG	4 cut(s)	104, 109, 575, 614
SduI	GDGCHC	8 cut(s)	285, 324, 366, 600, 982, 990, 1085, 1417
SfaNI	GCATC	5 cut(s)	433, 704, 769, 847, 1291
SfcI	CTRYAG	1 cut(s)	1311
SfoI	GGCGCC	1 cut(s)	292
Sfr303I	CCGCGG	1 cut(s)	159
SgrBI	CCGCGG	1 cut(s)	159
SinI	GGWCC	1 cut(s)	398
SmiMI	CAYNNNNRTG	2 cut(s)	371, 1341
SphI	GCATGC	1 cut(s)	1188
Sse9I	AATT	4 cut(s)	753, 920, 1034, 1158
SsiI	CCGC	8 cut(s)	153, 156, 158, 341, 351, 533, 718, 1432
SspDI	GGCGCC	1 cut(s)	290
SspMI	CTAG	1 cut(s)	8
SstI	GAGCTC	4 cut(s)	324, 600, 1085, 1417
StyD4I	CCNGG	4 cut(s)	102, 107, 573, 612
StyI	CCWWGG	3 cut(s)	165, 174, 1003
TaaI	ACNGT	2 cut(s)	238, 332
TasI	AATT	4 cut(s)	753, 920, 1034, 1158
TatI	WGTACW	3 cut(s)	180, 561, 1014
TauI	GCSGC	2 cut(s)	158, 161
TfiI	GAWTC	2 cut(s)	303, 1280
Tru1I	TTAA	2 cut(s)	957, 1029
Tru9I	TTAA	2 cut(s)	957, 1029
TscAI	CASTG	3 cut(s)	292, 373, 1399
TseFI	GTSAC	3 cut(s)	253, 332, 1141
TseI	GCWGC	5 cut(s)	79, 639, 911, 1292, 1310
Tsp45I	GTSAC	3 cut(s)	253, 332, 1141
TspDTI	ATGAA	2 cut(s)	813, 1015
TspGWI	ACGGA	5 cut(s)	115, 127, 990, 1369, 1503
TspRI	CASTG	3 cut(s)	292, 373, 1399
Van91I	CCANNNNNTGG	1 cut(s)	1241
VpaK11BI	GGWCC	1 cut(s)	398
XapI	RAATTY	2 cut(s)	920, 1034
XceI	RCATGY	1 cut(s)	1188
XcmI	CCANNNNNNNNNTGG	1 cut(s)	502
XmiI	GTMKAC	1 cut(s)	345
XmnI	GAANNNNTTC	2 cut(s)	1170, 1284
XspI	CTAG	1 cut(s)	8

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.