Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0012756

Physical Location & Seq

Genomic Coordinates Forward (+)

7341483 .. 7345615

Gene Structure 4133 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g12990.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGTACCCGAATCCAAACAAAAGCAGCCCAATAGGCCCACCTAAATCCCTCCATCAACCTGAAGTAGCAGTTAAAAGTACCCCACAGAGAAGAGTATATAAAACATGCACACCTACCTACCCAGCTGCCTCGCTTGGCATTCTCAACTGGCTCAAGAGACCCCTCCTTTATTTTCATCGCTCCTCGGCGGACTTTCCCACGCTTCTCCTTTTATATATCCTCCTACCTTTCCTCTCCCTCCAAACCCCTTCTCTCTCTCTTTCCTTTTCAAACAAACAAAAAATGGCTATGGCCACCCAACCCTCCACCACCCTCCTCCAGAACCCCTTCATTTCCTCCAAACCAAAGCTCTCCGCCGCCGCGATCCCTCTCCCTCCACGGCGCCGTTCCCTCTCCGCTCAATGCTCCGTCGCCGTCGCGCCGTCCGCCGCGGTTAGAGCTTCCAAGGAGTACAAGGTGAAGTCGGTGAAGGCGAGGCAGATTATTGACAGCAGGGGGAATCCGACGGTCGAGGTTGATCTGGTCACTGATGATCTGTACCGATCGGCCGTTCCTAGTGGGGCCTCTACCGGAATCTACGAGGCGTTGGAGCTTAGGGACGGTGATAAGGCTGTTTACGGCGGCAAGGGTGTGCTTAATGCTGTTAGGAATATCAATGACATTTTGGGGCCTAAGCTTATTGGCATCGATGTCAGAAATCAAAATGCTGTGGATGCGGTCATGTTGGAAATAGACGGAACACCAAACAAGTCTAAACTGGGGGCCAATGCCATATTGGGAGTGTCTCTTAGTGTGTGCAGAGCAGGTGCAGGGGCAAAGGGAATTCCCCTGTACAAGCACATCCAGGAAGTGTCTGGAACAAAAGAGCTTGTTATGCCCGTTCCGGCTTTTAATGTCATAAATGGAGGAAGCCATGCTGGAAACAATCTCGCCATGCAAGAGTTTATGATATTGCCTGTTGGTGCCTCCTCATTTGCCGAGGCACTACGGATGGGTAGTGAAGTTTATCACATACTAAAGGGAATTATCAAGAAAAAGTATGGACAAGATGCTTGCAATGTTGGAGATGAAGGGGGATTTGCTCCCAATGTACAAGATAACAGGGAAGGATTGGTTTTGCTCATTGATGCTATTGAAAAGGCTGGTTACACTGGCAAGATCAAAATCGGAATGGATGTTGCTGCTTCAGAATTTCTCACCAAGGATGGCAACTATGATCTTAACTTTAAGAAACAACCAAATGACGGGGCACATGTGCGCTCACCTCAGAGCCTTGGCGACCTGTACAAGGAGTTTATCAGAGATTTCCCTATTGTGTCCATTGAAGATCCATTTGACCAAGATGATTGGAGCTCATGGTCTTCACTTCAATCTTCAGTTGATATCCAAATTGTAGGAGATGACTTATTGGTCACAAATCCAAAGAAGATTTCTGAAGCCATTGGGAAGAAGGCATGCAATGCCTTGCTTTTGAAGGTAAACCAGATCGGCACAATCACTGAATCTATTCAGGCAGCACTTGACTCAAAAGCTGCTGGCTGGGGTGTGATGGTTAGTCACAGGAGTGGGGAAACTGAGGATAACTTCATTGCTGATCTCTCTGTTGGCTTGGCCAGTGGACAGATTAAGACAGGAGCTCCTTGCCGAAGTGAGCGTTTGGCCAAGTATAACCAGCTTCTGCGTATAGAAGAGGAACTTGGTAATGTCCGTTATGCTGGTGAAGCTTTCAGAGCTCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004634 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010090 GO:0016043 GO:0016829 GO:0016835 GO:0016836 GO:0030154 GO:0032502 GO:0032989 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0071840 GO:0090558 GO:0090626

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

580

Amino Acids

62.38

Weight (kDa)

8.91

Isoelectric Point (pI)

45.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	153 - 281	3.2e-55	Enolase, N-terminal domain
Enolase_C	PF00113	290 - 493	2.1e-95	Enolase, C-terminal TIM barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74030
fragaria_vesca	FvH4_5g12990 FvH4_5g12990 FvH4_5g12990
malus_domestica	MD06G1208300.v1.1 MD14G1218700.v1.1
prunus_persica	Prupe.5G212800_v2.0.a1
pyrus_communis	pycom06g18610
rosa_chinensis	RchiOBHm_Chr7g0184181
rosa_laevigata	RLG00000004996
rosa_multiflora	Rmu_sc0012221.1_g000006
rosa_roxburghii	Rroxscaffold_3G00269820
rosa_rugosa	Rorug06G0463700
rosa_samantha	Rh7AG082200 Rh7BG065000 Rh7CG065400 Rh7DG065100
rosa_wichuraiana	Rw7G005330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	794
AasI	GACNNNNNNGTC	1 cut(s)	1409
Acc36I	ACCTGC	1 cut(s)	794
AccB1I	GGYRCC	2 cut(s)	381, 962
AccBSI	CCGCTC	1 cut(s)	398
AccII	CGCG	3 cut(s)	362, 419, 431
AclWI	GGATC	2 cut(s)	358, 1322
AcoI	YGGCCR	4 cut(s)	291, 546, 1611, 1659
AcsI	RAATTY	2 cut(s)	822, 1190
AcuI	CTGAAG	4 cut(s)	81, 1170, 1359, 1455
AcyI	GRCGYC	1 cut(s)	382
AfaI	GTAC	7 cut(s)	5, 79, 452, 539, 833, 1092, 1286
AfiI	CCNNNNNNNGG	2 cut(s)	1244, 1288
AflIII	ACRYGT	1 cut(s)	1252
AgsI	TTSAA	5 cut(s)	270, 1136, 1325, 1370, 1474
AjnI	CCWGG	1 cut(s)	843
AjuI	GAANNNNNNNTTGG	4 cut(s)	1414, 1446, 1680, 1712
AleI	CACNNNNGTG	1 cut(s)	1563
Alw21I	GWGCWC	3 cut(s)	1355, 1639, 1735
Alw26I	GTCTC	2 cut(s)	151, 789
AlwI	GGATC	2 cut(s)	358, 1322
AlwNI	CAGNNNCTG	1 cut(s)	1678
AoxI	GGCC	8 cut(s)	34, 291, 546, 561, 668, 762, 1611, 1659
ApeKI	GCWGC	5 cut(s)	24, 125, 1181, 1514, 1532
ApoI	RAATTY	2 cut(s)	822, 1190
Asp700I	GAANNNNTTC	2 cut(s)	326, 1506
AspLEI	GCGC	3 cut(s)	384, 421, 1260
AspS9I	GGNCC	4 cut(s)	35, 561, 668, 762
AsuHPI	GGTGA	6 cut(s)	469, 478, 614, 1189, 1254, 1730
BaeGI	GKGCMC	1 cut(s)	1252
BalI	TGGCCA	3 cut(s)	293, 1613, 1661
BanI	GGYRCC	2 cut(s)	381, 962
BanII	GRGCYC	3 cut(s)	1355, 1639, 1735
BbsI	GAAGAC	1 cut(s)	1353
Bbv12I	GWGCWC	3 cut(s)	1355, 1639, 1735
BbvI	GCAGC	5 cut(s)	36, 112, 1168, 1519, 1526
BccI	CCATC	4 cut(s)	60, 985, 1199, 1543
BceAI	ACGGC	6 cut(s)	369, 395, 398, 406, 533, 634
BciT130I	CCWGG	1 cut(s)	845
BcoDI	GTCTC	2 cut(s)	151, 789
BfaI	CTAG	1 cut(s)	555
BfoI	RGCGCY	1 cut(s)	385
BfuAI	ACCTGC	1 cut(s)	794
BglI	GCCNNNNNGGC	1 cut(s)	33
BisI	GCNGC	9 cut(s)	25, 126, 357, 360, 429, 622, 1182, 1515, 1533
BlsI	GCNGC	9 cut(s)	26, 127, 358, 361, 430, 623, 1183, 1516, 1534
Bme1390I	CCNGG	1 cut(s)	845
BmgT120I	GGNCC	4 cut(s)	35, 561, 668, 762
BmiI	GGNNCC	5 cut(s)	383, 562, 669, 763, 964
BmrFI	CCNGG	1 cut(s)	845
BmrI	ACTGGG	1 cut(s)	767
BmsI	GCATC	4 cut(s)	693, 703, 1039, 1117
BmuI	ACTGGG	1 cut(s)	767
BpiI	GAAGAC	1 cut(s)	1353
BpmI	CTGGAG	1 cut(s)	302
Bpu10I	CCTNAGC	2 cut(s)	593, 672
BpuEI	CTTGAG	1 cut(s)	137
Bsa29I	ATCGAT	1 cut(s)	687
BsaBI	GATNNNNATC	1 cut(s)	1163
BsaHI	GRCGYC	1 cut(s)	382
BsaI	GGTCTC	1 cut(s)	151
BsaJI	CCNNGG	7 cut(s)	183, 377, 429, 444, 978, 1200, 1273
BsaWI	WCCGGW	1 cut(s)	569
BsaXI	ACNNNNNCTCC	2 cut(s)	1621, 1651
Bsc4I	CCNNNNNNNGG	2 cut(s)	1244, 1288
Bse1I	ACTGG	4 cut(s)	152, 762, 1156, 1614
Bse3DI	GCAATG	3 cut(s)	1063, 1465, 1587
Bse8I	GATNNNNATC	1 cut(s)	1163
BseBI	CCWGG	1 cut(s)	845
BseCI	ATCGAT	1 cut(s)	687
BseDI	CCNNGG	7 cut(s)	183, 377, 429, 444, 978, 1200, 1273
BseGI	GGATG	5 cut(s)	718, 840, 996, 1180, 1210
BseJI	GATNNNNATC	1 cut(s)	1163
BseLI	CCNNNNNNNGG	2 cut(s)	1244, 1288
BseMI	GCAATG	3 cut(s)	1063, 1465, 1587
BseMII	CTCAG	2 cut(s)	1280, 1566
BseNI	ACTGG	4 cut(s)	152, 762, 1156, 1614
BseRI	GAGGAG	3 cut(s)	172, 305, 958
BseSI	GKGCMC	1 cut(s)	1252
BseX3I	CGGCCG	1 cut(s)	546
BseXI	GCAGC	5 cut(s)	36, 112, 1168, 1519, 1526
BseYI	CCCAGC	2 cut(s)	121, 1539
BsgI	GTGCAG	2 cut(s)	817, 828
Bsh1236I	CGCG	3 cut(s)	362, 419, 431
Bsh1285I	CGRYCG	3 cut(s)	510, 545, 549
BshFI	GGCC	8 cut(s)	36, 293, 548, 563, 670, 764, 1613, 1661
BshNI	GGYRCC	2 cut(s)	381, 962
BshVI	ATCGAT	1 cut(s)	687
BsiEI	CGRYCG	3 cut(s)	510, 545, 549
BsiHKAI	GWGCWC	3 cut(s)	1355, 1639, 1735
BsiSI	CCGG	2 cut(s)	570, 884
BslFI	GGGAC	1 cut(s)	611
BslI	CCNNNNNNNGG	2 cut(s)	1244, 1288
BsmAI	GTCTC	2 cut(s)	151, 789
BsmFI	GGGAC	1 cut(s)	611
BsmI	GAATGC	1 cut(s)	138
BsnI	GGCC	8 cut(s)	36, 293, 548, 563, 670, 764, 1613, 1661
Bso31I	GGTCTC	1 cut(s)	151
Bsp1286I	GDGCHC	4 cut(s)	1252, 1355, 1639, 1735
Bsp1407I	TGTACA	3 cut(s)	831, 1090, 1284
Bsp143I	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
BspANI	GGCC	8 cut(s)	36, 293, 548, 563, 670, 764, 1613, 1661
BspCNI	CTCAG	2 cut(s)	1279, 1567
BspDI	ATCGAT	1 cut(s)	687
BspFNI	CGCG	3 cut(s)	362, 419, 431
BspLI	GGNNCC	5 cut(s)	383, 562, 669, 763, 964
BspMI	ACCTGC	1 cut(s)	794
BspPI	GGATC	2 cut(s)	358, 1322
BspT107I	GGYRCC	2 cut(s)	381, 962
BspTNI	GGTCTC	1 cut(s)	151
BsrBI	CCGCTC	1 cut(s)	398
BsrDI	GCAATG	3 cut(s)	1063, 1465, 1587
BsrGI	TGTACA	3 cut(s)	831, 1090, 1284
BsrI	ACTGG	4 cut(s)	152, 762, 1156, 1614
BssECI	CCNNGG	7 cut(s)	183, 377, 429, 444, 978, 1200, 1273
BssMI	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
BssNI	GRCGYC	1 cut(s)	382
BssT1I	CCWWGG	3 cut(s)	444, 1200, 1273
Bst2UI	CCWGG	1 cut(s)	845
Bst4CI	ACNGT	2 cut(s)	508, 602
Bst6I	CTCTTC	2 cut(s)	85, 1683
BstACI	GRCGYC	1 cut(s)	382
BstAPI	GCANNNNNTGC	1 cut(s)	1460
BstAUI	TGTACA	3 cut(s)	831, 1090, 1284
BstC8I	GCNNGC	3 cut(s)	1054, 1456, 1537
BstDEI	CTNAG	5 cut(s)	593, 672, 788, 1266, 1575
BstDSI	CCRYGG	2 cut(s)	377, 429
BstENI	CCTNNNNNAGG	1 cut(s)	1286
BstF5I	GGATG	5 cut(s)	718, 840, 996, 1180, 1210
BstFNI	CGCG	3 cut(s)	362, 419, 431
BstH2I	RGCGCY	1 cut(s)	385
BstHHI	GCGC	3 cut(s)	384, 421, 1260
BstKTI	GATC	9 cut(s)	366, 520, 535, 545, 1161, 1219, 1330, 1488, 1597
BstMAI	GTCTC	2 cut(s)	151, 789
BstMBI	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
BstMCI	CGRYCG	3 cut(s)	510, 545, 549
BstMWI	GCNNNNNNNGC	8 cut(s)	33, 425, 437, 713, 874, 1460, 1721, 1730
BstNI	CCWGG	1 cut(s)	845
BstNSI	RCATGY	3 cut(s)	108, 1256, 1458
BstSCI	CCNGG	1 cut(s)	843
BstSLI	GKGCMC	1 cut(s)	1252
BstUI	CGCG	3 cut(s)	362, 419, 431
BstV1I	GCAGC	5 cut(s)	36, 112, 1168, 1519, 1526
BstV2I	GAAGAC	1 cut(s)	1353
BstX2I	RGATCY	1 cut(s)	1327
BstYI	RGATCY	1 cut(s)	1327
BstZI	CGGCCG	1 cut(s)	546
Bsu15I	ATCGAT	1 cut(s)	687
BsuRI	GGCC	8 cut(s)	36, 293, 548, 563, 670, 764, 1613, 1661
BsuTUI	ATCGAT	1 cut(s)	687
BtgI	CCRYGG	2 cut(s)	377, 429
BtgZI	GCGATG	1 cut(s)	161
BtsCI	GGATG	5 cut(s)	718, 840, 996, 1180, 1210
BtsIMutI	CAGTG	4 cut(s)	525, 1149, 1497, 1621
BveI	ACCTGC	1 cut(s)	794
Cac8I	GCNNGC	3 cut(s)	1054, 1456, 1537
CaiI	CAGNNNCTG	1 cut(s)	1678
CfoI	GCGC	3 cut(s)	384, 421, 1260
Cfr13I	GGNCC	4 cut(s)	35, 561, 668, 762
Cfr42I	CCGCGG	1 cut(s)	432
ClaI	ATCGAT	1 cut(s)	687
Csp6I	GTAC	7 cut(s)	4, 78, 451, 538, 832, 1091, 1285
CviAII	CATG	8 cut(s)	105, 721, 914, 934, 1253, 1356, 1455, 1737
CviQI	GTAC	7 cut(s)	4, 78, 451, 538, 832, 1091, 1285
DdeI	CTNAG	5 cut(s)	593, 672, 788, 1266, 1575
DinI	GGCGCC	1 cut(s)	383
DpnI	GATC	9 cut(s)	365, 519, 534, 544, 1160, 1218, 1329, 1487, 1596
DpnII	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
DrdI	GACNNNNNNGTC	1 cut(s)	1409
DseDI	GACNNNNNNGTC	1 cut(s)	1409
EaeI	YGGCCR	4 cut(s)	291, 546, 1611, 1659
EagI	CGGCCG	1 cut(s)	546
Eam1104I	CTCTTC	2 cut(s)	85, 1683
EarI	CTCTTC	2 cut(s)	85, 1683
EciI	GGCGGA	3 cut(s)	203, 343, 415
Ecl136II	GAGCTC	3 cut(s)	1353, 1637, 1733
EclXI	CGGCCG	1 cut(s)	546
Eco130I	CCWWGG	3 cut(s)	444, 1200, 1273
Eco24I	GRGCYC	3 cut(s)	1355, 1639, 1735
Eco31I	GGTCTC	1 cut(s)	151
Eco32I	GATATC	1 cut(s)	1384
Eco52I	CGGCCG	1 cut(s)	546
Eco53kI	GAGCTC	3 cut(s)	1353, 1637, 1733
Eco57I	CTGAAG	4 cut(s)	81, 1170, 1359, 1455
EcoICRI	GAGCTC	3 cut(s)	1353, 1637, 1733
EcoNI	CCTNNNNNAGG	1 cut(s)	1286
EcoO109I	RGGNCCY	2 cut(s)	561, 668
EcoRI	GAATTC	1 cut(s)	822
EcoRII	CCWGG	1 cut(s)	843
EcoRV	GATATC	1 cut(s)	1384
EcoT14I	CCWWGG	3 cut(s)	444, 1200, 1273
EcoT38I	GRGCYC	3 cut(s)	1355, 1639, 1735
EgeI	GGCGCC	1 cut(s)	383
EheI	GGCGCC	1 cut(s)	383
ErhI	CCWWGG	3 cut(s)	444, 1200, 1273
FaeI	CATG	8 cut(s)	108, 724, 917, 937, 1256, 1359, 1458, 1740
FalI	AAGNNNNNCTT	2 cut(s)	1680, 1712
FaqI	GGGAC	1 cut(s)	611
FatI	CATG	8 cut(s)	104, 720, 913, 933, 1252, 1355, 1454, 1736
Fnu4HI	GCNGC	9 cut(s)	25, 126, 357, 360, 429, 622, 1182, 1515, 1533
FokI	GGATG	5 cut(s)	725, 827, 1003, 1187, 1217
FriOI	GRGCYC	3 cut(s)	1355, 1639, 1735
Fsp4HI	GCNGC	9 cut(s)	25, 126, 357, 360, 429, 622, 1182, 1515, 1533
FspBI	CTAG	1 cut(s)	555
GlaI	GCGC	3 cut(s)	383, 420, 1259
GluI	GCNGC	9 cut(s)	25, 126, 357, 360, 429, 622, 1182, 1515, 1533
GsaI	CCCAGC	2 cut(s)	125, 1543
GsuI	CTGGAG	1 cut(s)	302
HaeII	RGCGCY	1 cut(s)	385
HaeIII	GGCC	8 cut(s)	36, 293, 548, 563, 670, 764, 1613, 1661
HapII	CCGG	2 cut(s)	570, 884
HhaI	GCGC	3 cut(s)	384, 421, 1260
Hin1I	GRCGYC	1 cut(s)	382
Hin1II	CATG	8 cut(s)	108, 724, 917, 937, 1256, 1359, 1458, 1740
Hin6I	GCGC	3 cut(s)	382, 419, 1258
HinP1I	GCGC	3 cut(s)	382, 419, 1258
HindIII	AAGCTT	2 cut(s)	674, 1722
HinfI	GANTC	5 cut(s)	10, 499, 573, 1502, 1523
HpaII	CCGG	2 cut(s)	570, 884
HphI	GGTGA	6 cut(s)	469, 478, 614, 1189, 1254, 1730
Hpy166II	GTNNAC	3 cut(s)	616, 1480, 1619
Hpy188I	TCNGA	8 cut(s)	504, 695, 1169, 1189, 1269, 1301, 1435, 1730
Hpy188III	TCNNGA	4 cut(s)	154, 319, 855, 1030
Hpy8I	GTNNAC	3 cut(s)	616, 1480, 1619
Hpy99I	CGWCG	3 cut(s)	413, 419, 508
HpyAV	CCTTC	7 cut(s)	258, 337, 463, 1064, 1100, 1444, 1468
HpyCH4III	ACNGT	2 cut(s)	508, 602
HpyCH4V	TGCA	6 cut(s)	108, 798, 809, 937, 1056, 1458
HpyF10VI	GCNNNNNNNGC	8 cut(s)	33, 425, 437, 713, 874, 1460, 1721, 1730
HpyF3I	CTNAG	5 cut(s)	593, 672, 788, 1266, 1575
Hsp92I	GRCGYC	1 cut(s)	382
Hsp92II	CATG	8 cut(s)	108, 724, 917, 937, 1256, 1359, 1458, 1740
HspAI	GCGC	3 cut(s)	382, 419, 1258
KasI	GGCGCC	1 cut(s)	381
KspI	CCGCGG	1 cut(s)	432
Kzo9I	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
LmnI	GCTCC	8 cut(s)	185, 410, 589, 1087, 1350, 1634, 1642, 1738
Lsp1109I	GCAGC	5 cut(s)	36, 112, 1168, 1519, 1526
LweI	GCATC	4 cut(s)	693, 703, 1039, 1117
MaeI	CTAG	1 cut(s)	555
MaeIII	GTNAC	4 cut(s)	523, 1145, 1411, 1556
MalI	GATC	9 cut(s)	365, 519, 534, 544, 1160, 1218, 1329, 1487, 1596
MbiI	CCGCTC	1 cut(s)	398
MboI	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
MboII	GAAGA	7 cut(s)	102, 1337, 1353, 1365, 1438, 1459, 1700
MflI	RGATCY	1 cut(s)	1327
MhlI	GDGCHC	4 cut(s)	1252, 1355, 1639, 1735
MlsI	TGGCCA	3 cut(s)	293, 1613, 1661
MluCI	AATT	4 cut(s)	822, 1023, 1190, 1389
MluNI	TGGCCA	3 cut(s)	293, 1613, 1661
Mly113I	GGCGCC	1 cut(s)	382
MlyI	GAGTC	1 cut(s)	1517
MmeI	TCCRAC	4 cut(s)	527, 567, 705, 1042
Mox20I	TGGCCA	3 cut(s)	293, 1613, 1661
MroXI	GAANNNNTTC	2 cut(s)	326, 1506
MscI	TGGCCA	3 cut(s)	293, 1613, 1661
MseI	TTAA	6 cut(s)	72, 636, 891, 1221, 1227, 1626
MslI	CAYNNNNRTG	1 cut(s)	1563
Msp20I	TGGCCA	3 cut(s)	293, 1613, 1661
MspA1I	CMGCKG	2 cut(s)	125, 431
MspI	CCGG	2 cut(s)	570, 884
MspR9I	CCNGG	1 cut(s)	845
Mva1269I	GAATGC	1 cut(s)	138
MvaI	CCWGG	1 cut(s)	845
MvnI	CGCG	3 cut(s)	362, 419, 431
MwoI	GCNNNNNNNGC	8 cut(s)	33, 425, 437, 713, 874, 1460, 1721, 1730
NarI	GGCGCC	1 cut(s)	382
NdeII	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
NlaIII	CATG	8 cut(s)	108, 724, 917, 937, 1256, 1359, 1458, 1740
NlaIV	GGNNCC	5 cut(s)	383, 562, 669, 763, 964
NmeAIII	GCCGAG	2 cut(s)	164, 1003
NmuCI	GTSAC	3 cut(s)	523, 1411, 1556
NspI	RCATGY	3 cut(s)	108, 1256, 1458
OliI	CACNNNNGTG	1 cut(s)	1563
PaeI	GCATGC	1 cut(s)	1458
PaqCI	CACCTGC	1 cut(s)	794
PciI	ACATGT	1 cut(s)	1252
PcsI	WCGNNNNNNNCGW	1 cut(s)	470
PctI	GAATGC	1 cut(s)	138
PdmI	GAANNNNTTC	2 cut(s)	326, 1506
PfeI	GAWTC	4 cut(s)	10, 499, 573, 1502
PfoI	TCCNGGA	1 cut(s)	843
PkrI	GCNGC	9 cut(s)	26, 127, 358, 361, 430, 623, 1183, 1516, 1534
Ple19I	CGATCG	1 cut(s)	545
PleI	GAGTC	1 cut(s)	1517
PluTI	GGCGCC	1 cut(s)	385
PpsI	GAGTC	1 cut(s)	1517
PscI	ACATGT	1 cut(s)	1252
Psp124BI	GAGCTC	3 cut(s)	1355, 1639, 1735
Psp6I	CCWGG	1 cut(s)	843
PspFI	CCCAGC	2 cut(s)	121, 1539
PspGI	CCWGG	1 cut(s)	843
PspN4I	GGNNCC	5 cut(s)	383, 562, 669, 763, 964
PspPI	GGNCC	4 cut(s)	35, 561, 668, 762
PstNI	CAGNNNCTG	1 cut(s)	1678
PsuI	RGATCY	1 cut(s)	1327
PvuI	CGATCG	1 cut(s)	545
PvuII	CAGCTG	1 cut(s)	125
RsaI	GTAC	7 cut(s)	5, 79, 452, 539, 833, 1092, 1286
RsaNI	GTAC	7 cut(s)	4, 78, 451, 538, 832, 1091, 1285
RseI	CAYNNNNRTG	1 cut(s)	1563
SacI	GAGCTC	3 cut(s)	1355, 1639, 1735
SacII	CCGCGG	1 cut(s)	432
SaqAI	TTAA	6 cut(s)	72, 636, 891, 1221, 1227, 1626
SatI	GCNGC	9 cut(s)	25, 126, 357, 360, 429, 622, 1182, 1515, 1533
Sau3AI	GATC	9 cut(s)	363, 517, 532, 542, 1158, 1216, 1327, 1485, 1594
Sau96I	GGNCC	4 cut(s)	35, 561, 668, 762
SchI	GAGTC	1 cut(s)	1517
ScrFI	CCNGG	1 cut(s)	845
SduI	GDGCHC	4 cut(s)	1252, 1355, 1639, 1735
SfaNI	GCATC	4 cut(s)	693, 703, 1039, 1117
SfoI	GGCGCC	1 cut(s)	383
Sfr303I	CCGCGG	1 cut(s)	432
SgrBI	CCGCGG	1 cut(s)	432
SmiMI	CAYNNNNRTG	1 cut(s)	1563
SmlI	CTYRAG	1 cut(s)	152
SmoI	CTYRAG	1 cut(s)	152
SphI	GCATGC	1 cut(s)	1458
Sse9I	AATT	4 cut(s)	822, 1023, 1190, 1389
SspDI	GGCGCC	1 cut(s)	381
SspMI	CTAG	1 cut(s)	555
SstI	GAGCTC	3 cut(s)	1355, 1639, 1735
StyD4I	CCNGG	1 cut(s)	843
StyI	CCWWGG	3 cut(s)	444, 1200, 1273
TaaI	ACNGT	2 cut(s)	508, 602
TaqI	TCGA	2 cut(s)	510, 687
TasI	AATT	4 cut(s)	822, 1023, 1190, 1389
TatI	WGTACW	4 cut(s)	450, 831, 1090, 1284
TauI	GCSGC	4 cut(s)	359, 362, 431, 624
TfiI	GAWTC	4 cut(s)	10, 499, 573, 1502
Tru1I	TTAA	6 cut(s)	72, 636, 891, 1221, 1227, 1626
Tru9I	TTAA	6 cut(s)	72, 636, 891, 1221, 1227, 1626
TscAI	CASTG	4 cut(s)	532, 1156, 1504, 1621
TseFI	GTSAC	3 cut(s)	523, 1411, 1556
TseI	GCWGC	5 cut(s)	24, 125, 1181, 1514, 1532
Tsp45I	GTSAC	3 cut(s)	523, 1411, 1556
TspDTI	ATGAA	4 cut(s)	164, 319, 1083, 1576
TspGWI	ACGGA	4 cut(s)	397, 750, 1003, 1697
TspRI	CASTG	4 cut(s)	532, 1156, 1504, 1621
XagI	CCTNNNNNAGG	1 cut(s)	1286
XapI	RAATTY	2 cut(s)	822, 1190
XceI	RCATGY	3 cut(s)	108, 1256, 1458
XcmI	CCANNNNNNNNNTGG	2 cut(s)	772, 851
XmnI	GAANNNNTTC	2 cut(s)	326, 1506
XspI	CTAG	1 cut(s)	555

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.