Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012756

Physical Location & Seq

Genomic Coordinates Reverse (-)

30158750 .. 30161427

Gene Structure 2678 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1218700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGGCTCTAGCTACCCAGCCCACCACCAACCTCCTCCAAAACCCCTTCCTCTGCTCCAAACCCCAGCCCAGACCCCAGCTCCCCACCGGCGTCCCCTGCCTGGCCCGGCGTCGTTCTGGCTCCGTCCAATGCTCAGTCGCCGTCGCGCCCACCGCGGCCGCCAAGGCCTCCAAGGAGTACAAGGTCAAGTCGGTGAAGGCGAGGCAGATCATCGACAGCAGGGGTAATCCGACTGTCGAGGTTGATCTGGTCACTGACGATCTGTACCGATCGGCCGTGCCCAGTGGCGCCTCCACCGGAATTTACGAGGCGTTGGAGCTCAGAGACGGTGACAAGGCTGTCTACGGCGGGAAAGGTGTGCTCACTGCTGTGAAGAACATCAATGAGATTTTGGGACCCAAGCTCGTTGGCGTCGATGTCAGAAATCAAAATGAGGTTGATGCACTTATGTTGGAAATCGATGGAACCCCAAACAAGTCGAAACTGGGGGCCAATGCCATATTGGGAGTATCTCTGAGCGTGTGTAGAGCGGGAGCAGGAGCAAAGGGAGTGCCATTGTACAAGCACATCCAGGAAGTTTCAGGAACGAAGGAGCTTGTTATGCCCGTCCCAGCTTTCAATGTGATAAATGGAGGCAGCCACGCAGGAAATAATCTGGCAATGCAGGAGTTTATGATCTTGCCTGTTGGTGCATCCTCGTTCGCTGAGGCACTCCGCATGGGTAGTGAAGTTTATCATACACTAAAGGGAATTATCAAGGCAAAATATGGACAGGATGCTTGCAATGTTGGAGATGAAGGGGGGTTTGCTCCCAATGTGCAGGATAATAGGGAGGGTCTAGTTTTGCTCATTGATGCTATTGAAAAGGCTGGATACACCGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAATTTCTCACCAAAGAAGGGAAATACGATCTCAACTTTAAGAAACAACCAAATGACGGGGCCCATGTGCTCTCGCCTCCGAACCTTGGGGAGCTGTACAAGGAGTTCATTAAGGAATTCCCAATTGTGTCTATTGAAGATCCATTTGACCAAGACGATTGGAGCTCGTGGTCTTCACTTCAGTCTTCTGTTGATATCCAACTTGTAGGAGATGACTTGTTGGTCACAAATCCAACAAAAATTGCTGAAGCCATTCAGAAGAAGGCATGCAATGGTTTACTTCTAAAGGCAAGCATATTTAACTGCGTCAATTGTTTTCCTTTAAATGATAGCCGACCCCACTTAGTGGGAAAAGGCTTTGTTGTTAACCAGATCGGCACAATAACTGAATCTATTCAGGCAGCACTCGACGCAAAAGCTGCAGGTTGGGGTGTTATGGTCAGCCACCGGAGTGGAGAAACTGAAGATAACTTTATCGCTGATCTTTCTGTTGGTTTGGCTAGTGGACAGATCAAGACAGGAGCTCCCTGCCGAAGTGAGCGGTTGGCCAAGTATAATCAGCTTCTGCGTATTGAAGAGGAACTCGGCAATGTGCGTTATGCTGGCGAAGCTTTCAGATCTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004634 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010090 GO:0016043 GO:0016829 GO:0016835 GO:0016836 GO:0030154 GO:0032502 GO:0032989 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0071840 GO:0090558 GO:0090626

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

514

Amino Acids

54.78

Weight (kDa)

6.16

Isoelectric Point (pI)

36.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	62 - 190	1.5e-55	Enolase, N-terminal domain
Enolase_C	PF00113	199 - 402	9.9e-96	Enolase, C-terminal TIM barrel domain
Enolase_C	PF00113	427 - 511	1.6e-41	Enolase, C-terminal TIM barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74030
fragaria_vesca	FvH4_5g12990 FvH4_5g12990 FvH4_5g12990
malus_domestica	MD06G1208300.v1.1 MD14G1218700.v1.1
prunus_persica	Prupe.5G212800_v2.0.a1
pyrus_communis	pycom06g18610
rosa_chinensis	RchiOBHm_Chr7g0184181
rosa_laevigata	RLG00000004996
rosa_multiflora	Rmu_sc0012221.1_g000006
rosa_roxburghii	Rroxscaffold_3G00269820
rosa_rugosa	Rorug06G0463700
rosa_samantha	Rh7AG082200 Rh7BG065000 Rh7CG065400 Rh7DG065100
rosa_wichuraiana	Rw7G005330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	338, 1136
Acc36I	ACCTGC	1 cut(s)	1328
AccB1I	GGYRCC	1 cut(s)	287
AccB7I	CCANNNNNTGG	1 cut(s)	1261
AccBSI	CCGCTC	2 cut(s)	530, 1456
AccI	GTMKAC	1 cut(s)	342
AccII	CGCG	2 cut(s)	146, 155
AciI	CCGC	7 cut(s)	153, 155, 159, 348, 530, 715, 1456
AclWI	GGATC	1 cut(s)	1049
AcoI	YGGCCR	3 cut(s)	156, 273, 1461
AcsI	RAATTY	3 cut(s)	300, 917, 1031
AcuI	CTGAAG	4 cut(s)	897, 1079, 1182, 1398
AcyI	GRCGYC	4 cut(s)	90, 109, 288, 411
AdeI	CACNNNGTG	1 cut(s)	1261
AfaI	GTAC	4 cut(s)	179, 266, 560, 1013
AfiI	CCNNNNNNNGG	5 cut(s)	100, 933, 971, 1001, 1261
AgsI	TTSAA	4 cut(s)	619, 863, 1052, 1490
AjnI	CCWGG	2 cut(s)	99, 570
AleI	CACNNNNGTG	2 cut(s)	368, 1365
Alw21I	GWGCWC	5 cut(s)	321, 363, 987, 1082, 1441
Alw26I	GTCTC	1 cut(s)	318
AlwI	GGATC	1 cut(s)	1049
AlwNI	CAGNNNCTG	1 cut(s)	1480
AoxI	GGCC	7 cut(s)	102, 156, 165, 273, 489, 975, 1461
ApaI	GGGCCC	1 cut(s)	979
ApeKI	GCWGC	4 cut(s)	636, 908, 1316, 1334
ApoI	RAATTY	3 cut(s)	300, 917, 1031
Asp700I	GAANNNNTTC	2 cut(s)	1167, 1308
AspLEI	GCGC	2 cut(s)	148, 290
AspS9I	GGNCC	5 cut(s)	103, 395, 489, 975, 976
AsuC2I	CCSGG	1 cut(s)	106
AsuHPI	GGTGA	3 cut(s)	205, 341, 916
AvaII	GGWCC	1 cut(s)	395
BaeGI	GKGCMC	2 cut(s)	282, 979
BalI	TGGCCA	1 cut(s)	1463
BanI	GGYRCC	1 cut(s)	287
BanII	GRGCYC	4 cut(s)	321, 979, 1082, 1441
BauI	CACGAG	1 cut(s)	1081
BbsI	GAAGAC	2 cut(s)	1080, 1092
Bbv12I	GWGCWC	5 cut(s)	321, 363, 987, 1082, 1441
BbvCI	CCTCAGC	1 cut(s)	705
BbvI	GCAGC	4 cut(s)	648, 920, 1321, 1328
BccI	CCATC	1 cut(s)	455
BceAI	ACGGC	3 cut(s)	125, 260, 361
BciT130I	CCWGG	2 cut(s)	101, 572
BciVI	GTATCC	1 cut(s)	866
BcnI	CCSGG	1 cut(s)	106
BcoDI	GTCTC	1 cut(s)	318
BfaI	CTAG	3 cut(s)	8, 839, 1416
BfmI	CTRYAG	1 cut(s)	1335
BfoI	RGCGCY	1 cut(s)	291
BfuAI	ACCTGC	1 cut(s)	1328
BfuI	GTATCC	1 cut(s)	866
BglI	GCCNNNNNGGC	1 cut(s)	164
BglII	AGATCT	1 cut(s)	1532
BisI	GCNGC	6 cut(s)	156, 159, 637, 909, 1317, 1335
BlsI	GCNGC	6 cut(s)	157, 160, 638, 910, 1318, 1336
Bme1390I	CCNGG	3 cut(s)	101, 106, 572
Bme18I	GGWCC	1 cut(s)	395
BmgT120I	GGNCC	5 cut(s)	103, 395, 489, 975, 976
BmiI	GGNNCC	8 cut(s)	121, 289, 396, 397, 466, 490, 976, 977
BmrFI	CCNGG	3 cut(s)	101, 106, 572
BmrI	ACTGGG	2 cut(s)	276, 494
BmsI	GCATC	4 cut(s)	430, 701, 766, 844
BmuI	ACTGGG	2 cut(s)	276, 494
BpiI	GAAGAC	2 cut(s)	1080, 1092
Bpu10I	CCTNAGC	1 cut(s)	705
BpuMI	CCSGG	1 cut(s)	106
Bsa29I	ATCGAT	1 cut(s)	459
BsaHI	GRCGYC	4 cut(s)	90, 109, 288, 411
BsaJI	CCNNGG	4 cut(s)	153, 162, 171, 1000
BsaWI	WCCGGW	3 cut(s)	296, 878, 1362
BsaXI	ACNNNNNCTCC	4 cut(s)	63, 93, 1423, 1453
Bsc4I	CCNNNNNNNGG	5 cut(s)	100, 933, 971, 1001, 1261
Bse118I	RCCGGY	1 cut(s)	86
Bse1I	ACTGG	2 cut(s)	282, 489
Bse3DI	GCAATG	4 cut(s)	666, 790, 1192, 1510
BseBI	CCWGG	2 cut(s)	101, 572
BseCI	ATCGAT	1 cut(s)	459
BseDI	CCNNGG	4 cut(s)	153, 162, 171, 1000
BseGI	GGATG	4 cut(s)	567, 692, 781, 907
BseLI	CCNNNNNNNGG	5 cut(s)	100, 933, 971, 1001, 1261
BseMI	GCAATG	4 cut(s)	666, 790, 1192, 1510
BseMII	CTCAG	4 cut(s)	147, 334, 506, 696
BseNI	ACTGG	2 cut(s)	282, 489
BseRI	GAGGAG	1 cut(s)	23
BseSI	GKGCMC	2 cut(s)	282, 979
BseX3I	CGGCCG	2 cut(s)	156, 273
BseXI	GCAGC	4 cut(s)	648, 920, 1321, 1328
BseYI	CCCAGC	4 cut(s)	15, 63, 75, 610
BsgI	GTGCAG	1 cut(s)	839
Bsh1236I	CGCG	2 cut(s)	146, 155
Bsh1285I	CGRYCG	3 cut(s)	159, 272, 276
BshFI	GGCC	7 cut(s)	104, 158, 167, 275, 491, 977, 1463
BshNI	GGYRCC	1 cut(s)	287
BshVI	ATCGAT	1 cut(s)	459
BsiEI	CGRYCG	3 cut(s)	159, 272, 276
BsiHKAI	GWGCWC	5 cut(s)	321, 363, 987, 1082, 1441
BsiSI	CCGG	5 cut(s)	87, 106, 297, 879, 1363
BslFI	GGGAC	3 cut(s)	77, 408, 593
BslI	CCNNNNNNNGG	5 cut(s)	100, 933, 971, 1001, 1261
BsmAI	GTCTC	1 cut(s)	318
BsmBI	CGTCTC	1 cut(s)	318
BsmFI	GGGAC	3 cut(s)	77, 408, 593
BsnI	GGCC	7 cut(s)	104, 158, 167, 275, 491, 977, 1463
Bsp120I	GGGCCC	1 cut(s)	975
Bsp1286I	GDGCHC	7 cut(s)	282, 321, 363, 979, 987, 1082, 1441
Bsp1407I	TGTACA	2 cut(s)	558, 1011
BspACI	CCGC	7 cut(s)	153, 155, 159, 348, 530, 715, 1456
BspANI	GGCC	7 cut(s)	104, 158, 167, 275, 491, 977, 1463
BspCNI	CTCAG	4 cut(s)	146, 333, 507, 697
BspDI	ATCGAT	1 cut(s)	459
BspFNI	CGCG	2 cut(s)	146, 155
BspLI	GGNNCC	8 cut(s)	121, 289, 396, 397, 466, 490, 976, 977
BspMAI	CTGCAG	1 cut(s)	1339
BspMI	ACCTGC	1 cut(s)	1328
BspPI	GGATC	1 cut(s)	1049
BspT107I	GGYRCC	1 cut(s)	287
BsrBI	CCGCTC	2 cut(s)	530, 1456
BsrDI	GCAATG	4 cut(s)	666, 790, 1192, 1510
BsrFI	RCCGGY	1 cut(s)	86
BsrGI	TGTACA	2 cut(s)	558, 1011
BsrI	ACTGG	2 cut(s)	282, 489
BssAI	RCCGGY	1 cut(s)	86
BssECI	CCNNGG	4 cut(s)	153, 162, 171, 1000
BssNI	GRCGYC	4 cut(s)	90, 109, 288, 411
BssSI	CACGAG	1 cut(s)	1081
BssT1I	CCWWGG	3 cut(s)	162, 171, 1000
Bst2BI	CACGAG	1 cut(s)	1081
Bst2UI	CCWGG	2 cut(s)	101, 572
Bst4CI	ACNGT	2 cut(s)	235, 329
Bst6I	CTCTTC	1 cut(s)	1485
BstACI	GRCGYC	4 cut(s)	90, 109, 288, 411
BstAPI	GCANNNNNTGC	1 cut(s)	1334
BstAUI	TGTACA	2 cut(s)	558, 1011
BstC8I	GCNNGC	4 cut(s)	781, 1183, 1207, 1519
BstDEI	CTNAG	5 cut(s)	133, 320, 515, 705, 1258
BstDSI	CCRYGG	1 cut(s)	153
BstF5I	GGATG	4 cut(s)	567, 692, 781, 907
BstFNI	CGCG	2 cut(s)	146, 155
BstH2I	RGCGCY	1 cut(s)	291
BstHHI	GCGC	2 cut(s)	148, 290
BstMAI	GTCTC	1 cut(s)	318
BstMCI	CGRYCG	3 cut(s)	159, 272, 276
BstMWI	GCNNNNNNNGC	7 cut(s)	96, 152, 164, 601, 1325, 1334, 1523
BstNI	CCWGG	2 cut(s)	101, 572
BstNSI	RCATGY	1 cut(s)	1185
BstSCI	CCNGG	3 cut(s)	99, 104, 570
BstSFI	CTRYAG	1 cut(s)	1335
BstSLI	GKGCMC	2 cut(s)	282, 979
BstUI	CGCG	2 cut(s)	146, 155
BstV1I	GCAGC	4 cut(s)	648, 920, 1321, 1328
BstV2I	GAAGAC	2 cut(s)	1080, 1092
BstX2I	RGATCY	2 cut(s)	1054, 1532
BstXI	CCANNNNNNTGG	1 cut(s)	1367
BstYI	RGATCY	2 cut(s)	1054, 1532
BstZI	CGGCCG	2 cut(s)	156, 273
Bsu15I	ATCGAT	1 cut(s)	459
BsuI	GTATCC	1 cut(s)	866
BsuRI	GGCC	7 cut(s)	104, 158, 167, 275, 491, 977, 1463
BsuTUI	ATCGAT	1 cut(s)	459
BtgI	CCRYGG	1 cut(s)	153
BtsCI	GGATG	4 cut(s)	567, 692, 781, 907
BtsI	GCAGTG	1 cut(s)	363
BtsIMutI	CAGTG	3 cut(s)	252, 289, 363
BveI	ACCTGC	1 cut(s)	1328
Cac8I	GCNNGC	4 cut(s)	781, 1183, 1207, 1519
CaiI	CAGNNNCTG	1 cut(s)	1480
CciNI	GCGGCCGC	1 cut(s)	156
CfoI	GCGC	2 cut(s)	148, 290
Cfr10I	RCCGGY	1 cut(s)	86
Cfr13I	GGNCC	5 cut(s)	103, 395, 489, 975, 976
Cfr42I	CCGCGG	1 cut(s)	156
ClaI	ATCGAT	1 cut(s)	459
CseI	GACGC	5 cut(s)	79, 98, 400, 1210, 1334
Csp6I	GTAC	4 cut(s)	178, 265, 559, 1012
CviAII	CATG	3 cut(s)	718, 980, 1182
CviQI	GTAC	4 cut(s)	178, 265, 559, 1012
DdeI	CTNAG	5 cut(s)	133, 320, 515, 705, 1258
DinI	GGCGCC	1 cut(s)	289
DraI	TTTAAA	1 cut(s)	1239
DraIII	CACNNNGTG	1 cut(s)	1261
DrdI	GACNNNNNNGTC	2 cut(s)	338, 1136
DseDI	GACNNNNNNGTC	2 cut(s)	338, 1136
EaeI	YGGCCR	3 cut(s)	156, 273, 1461
EagI	CGGCCG	2 cut(s)	156, 273
Eam1104I	CTCTTC	1 cut(s)	1485
EarI	CTCTTC	1 cut(s)	1485
Ecl136II	GAGCTC	3 cut(s)	319, 1080, 1439
EclXI	CGGCCG	2 cut(s)	156, 273
Eco130I	CCWWGG	3 cut(s)	162, 171, 1000
Eco147I	AGGCCT	1 cut(s)	167
Eco24I	GRGCYC	4 cut(s)	321, 979, 1082, 1441
Eco32I	GATATC	1 cut(s)	1111
Eco47I	GGWCC	1 cut(s)	395
Eco52I	CGGCCG	2 cut(s)	156, 273
Eco53kI	GAGCTC	3 cut(s)	319, 1080, 1439
Eco57I	CTGAAG	4 cut(s)	897, 1079, 1182, 1398
EcoICRI	GAGCTC	3 cut(s)	319, 1080, 1439
EcoO109I	RGGNCCY	2 cut(s)	395, 975
EcoRI	GAATTC	1 cut(s)	1031
EcoRII	CCWGG	2 cut(s)	99, 570
EcoRV	GATATC	1 cut(s)	1111
EcoT14I	CCWWGG	3 cut(s)	162, 171, 1000
EcoT38I	GRGCYC	4 cut(s)	321, 979, 1082, 1441
EgeI	GGCGCC	1 cut(s)	289
EheI	GGCGCC	1 cut(s)	289
ErhI	CCWWGG	3 cut(s)	162, 171, 1000
Esp3I	CGTCTC	1 cut(s)	318
FaeI	CATG	3 cut(s)	721, 983, 1185
FaqI	GGGAC	3 cut(s)	77, 408, 593
FatI	CATG	3 cut(s)	717, 979, 1181
FauI	CCCGC	2 cut(s)	341, 523
FblI	GTMKAC	1 cut(s)	342
Fnu4HI	GCNGC	6 cut(s)	156, 159, 637, 909, 1317, 1335
FokI	GGATG	4 cut(s)	554, 679, 788, 914
FriOI	GRGCYC	4 cut(s)	321, 979, 1082, 1441
Fsp4HI	GCNGC	6 cut(s)	156, 159, 637, 909, 1317, 1335
FspBI	CTAG	3 cut(s)	8, 839, 1416
GlaI	GCGC	2 cut(s)	147, 289
GluI	GCNGC	6 cut(s)	156, 159, 637, 909, 1317, 1335
GsaI	CCCAGC	4 cut(s)	19, 67, 79, 614
HaeII	RGCGCY	1 cut(s)	291
HaeIII	GGCC	7 cut(s)	104, 158, 167, 275, 491, 977, 1463
HapII	CCGG	5 cut(s)	87, 106, 297, 879, 1363
HgaI	GACGC	5 cut(s)	79, 98, 400, 1210, 1334
HhaI	GCGC	2 cut(s)	148, 290
Hin1I	GRCGYC	4 cut(s)	90, 109, 288, 411
Hin1II	CATG	3 cut(s)	721, 983, 1185
Hin6I	GCGC	2 cut(s)	146, 288
HinP1I	GCGC	2 cut(s)	146, 288
HincII	GTYRAC	1 cut(s)	1282
HindII	GTYRAC	1 cut(s)	1282
HindIII	AAGCTT	1 cut(s)	1524
HinfI	GANTC	1 cut(s)	1304
HpaI	GTTAAC	1 cut(s)	1282
HpaII	CCGG	5 cut(s)	87, 106, 297, 879, 1363
HphI	GGTGA	3 cut(s)	205, 341, 916
Hpy166II	GTNNAC	4 cut(s)	343, 1193, 1282, 1421
Hpy188I	TCNGA	8 cut(s)	231, 323, 422, 516, 916, 996, 1173, 1532
Hpy188III	TCNNGA	2 cut(s)	582, 1429
Hpy8I	GTNNAC	4 cut(s)	343, 1193, 1282, 1421
Hpy99I	CGWCG	4 cut(s)	114, 146, 416, 1328
HpyAV	CCTTC	6 cut(s)	55, 190, 583, 791, 926, 1171
HpyCH4III	ACNGT	2 cut(s)	235, 329
HpyCH4V	TGCA	8 cut(s)	443, 664, 692, 783, 820, 908, 1185, 1337
HpyF10VI	GCNNNNNNNGC	7 cut(s)	96, 152, 164, 601, 1325, 1334, 1523
HpyF3I	CTNAG	5 cut(s)	133, 320, 515, 705, 1258
Hsp92I	GRCGYC	4 cut(s)	90, 109, 288, 411
Hsp92II	CATG	3 cut(s)	721, 983, 1185
HspAI	GCGC	2 cut(s)	146, 288
KasI	GGCGCC	1 cut(s)	287
KflI	GGGWCCC	1 cut(s)	395
KspAI	GTTAAC	1 cut(s)	1282
KspI	CCGCGG	1 cut(s)	156
Lsp1109I	GCAGC	4 cut(s)	648, 920, 1321, 1328
LweI	GCATC	4 cut(s)	430, 701, 766, 844
MaeI	CTAG	3 cut(s)	8, 839, 1416
MaeIII	GTNAC	3 cut(s)	250, 329, 1138
MbiI	CCGCTC	2 cut(s)	530, 1456
MboII	GAAGA	7 cut(s)	385, 1064, 1080, 1092, 1186, 1391, 1502
MfeI	CAATTG	2 cut(s)	1038, 1225
MflI	RGATCY	2 cut(s)	1054, 1532
MhlI	GDGCHC	7 cut(s)	282, 321, 363, 979, 987, 1082, 1441
MlsI	TGGCCA	1 cut(s)	1463
MluCI	AATT	7 cut(s)	300, 750, 917, 1031, 1038, 1155, 1225
MluNI	TGGCCA	1 cut(s)	1463
Mly113I	GGCGCC	1 cut(s)	288
MmeI	TCCRAC	6 cut(s)	254, 294, 432, 769, 1138, 1172
Mox20I	TGGCCA	1 cut(s)	1463
MroXI	GAANNNNTTC	2 cut(s)	1167, 1308
MscI	TGGCCA	1 cut(s)	1463
MseI	TTAA	5 cut(s)	954, 1026, 1215, 1238, 1281
MslI	CAYNNNNRTG	2 cut(s)	368, 1365
Msp20I	TGGCCA	1 cut(s)	1463
MspA1I	CMGCKG	1 cut(s)	155
MspI	CCGG	5 cut(s)	87, 106, 297, 879, 1363
MspR9I	CCNGG	3 cut(s)	101, 106, 572
MunI	CAATTG	2 cut(s)	1038, 1225
MvaI	CCWGG	2 cut(s)	101, 572
MvnI	CGCG	2 cut(s)	146, 155
MwoI	GCNNNNNNNGC	7 cut(s)	96, 152, 164, 601, 1325, 1334, 1523
NarI	GGCGCC	1 cut(s)	288
NciI	CCSGG	1 cut(s)	106
NlaIII	CATG	3 cut(s)	721, 983, 1185
NlaIV	GGNNCC	8 cut(s)	121, 289, 396, 397, 466, 490, 976, 977
NmeAIII	GCCGAG	1 cut(s)	1479
NmuCI	GTSAC	3 cut(s)	250, 329, 1138
NotI	GCGGCCGC	1 cut(s)	156
NspI	RCATGY	1 cut(s)	1185
OliI	CACNNNNGTG	2 cut(s)	368, 1365
PaeI	GCATGC	1 cut(s)	1185
PceI	AGGCCT	1 cut(s)	167
PcsI	WCGNNNNNNNCGW	2 cut(s)	197, 411
PdmI	GAANNNNTTC	2 cut(s)	1167, 1308
PfeI	GAWTC	1 cut(s)	1304
PflMI	CCANNNNNTGG	1 cut(s)	1261
PfoI	TCCNGGA	1 cut(s)	570
PkrI	GCNGC	6 cut(s)	157, 160, 638, 910, 1318, 1336
Ple19I	CGATCG	1 cut(s)	272
PluTI	GGCGCC	1 cut(s)	291
PpuMI	RGGWCCY	1 cut(s)	395
Psp124BI	GAGCTC	3 cut(s)	321, 1082, 1441
Psp5II	RGGWCCY	1 cut(s)	395
Psp6I	CCWGG	2 cut(s)	99, 570
PspFI	CCCAGC	4 cut(s)	15, 63, 75, 610
PspGI	CCWGG	2 cut(s)	99, 570
PspN4I	GGNNCC	8 cut(s)	121, 289, 396, 397, 466, 490, 976, 977
PspOMI	GGGCCC	1 cut(s)	975
PspPI	GGNCC	5 cut(s)	103, 395, 489, 975, 976
PspPPI	RGGWCCY	1 cut(s)	395
PsrI	GAACNNNNNNTAC	2 cut(s)	1004, 1036
PstI	CTGCAG	1 cut(s)	1339
PstNI	CAGNNNCTG	1 cut(s)	1480
PsuI	RGATCY	2 cut(s)	1054, 1532
PvuI	CGATCG	1 cut(s)	272
RsaI	GTAC	4 cut(s)	179, 266, 560, 1013
RsaNI	GTAC	4 cut(s)	178, 265, 559, 1012
RseI	CAYNNNNRTG	2 cut(s)	368, 1365
SacI	GAGCTC	3 cut(s)	321, 1082, 1441
SacII	CCGCGG	1 cut(s)	156
SaqAI	TTAA	5 cut(s)	954, 1026, 1215, 1238, 1281
SatI	GCNGC	6 cut(s)	156, 159, 637, 909, 1317, 1335
Sau96I	GGNCC	5 cut(s)	103, 395, 489, 975, 976
ScrFI	CCNGG	3 cut(s)	101, 106, 572
SduI	GDGCHC	7 cut(s)	282, 321, 363, 979, 987, 1082, 1441
SfaNI	GCATC	4 cut(s)	430, 701, 766, 844
SfcI	CTRYAG	1 cut(s)	1335
SfiI	GGCCNNNNNGGCC	1 cut(s)	164
SfoI	GGCGCC	1 cut(s)	289
Sfr303I	CCGCGG	1 cut(s)	156
SgrAI	CRCCGGYG	1 cut(s)	86
SgrBI	CCGCGG	1 cut(s)	156
SinI	GGWCC	1 cut(s)	395
SmiMI	CAYNNNNRTG	2 cut(s)	368, 1365
SphI	GCATGC	1 cut(s)	1185
Sse9I	AATT	7 cut(s)	300, 750, 917, 1031, 1038, 1155, 1225
SseBI	AGGCCT	1 cut(s)	167
SsiI	CCGC	7 cut(s)	153, 155, 159, 348, 530, 715, 1456
SspDI	GGCGCC	1 cut(s)	287
SspMI	CTAG	3 cut(s)	8, 839, 1416
SstI	GAGCTC	3 cut(s)	321, 1082, 1441
StuI	AGGCCT	1 cut(s)	167
StyD4I	CCNGG	3 cut(s)	99, 104, 570
StyI	CCWWGG	3 cut(s)	162, 171, 1000
TaaI	ACNGT	2 cut(s)	235, 329
TaqI	TCGA	6 cut(s)	213, 237, 414, 459, 479, 1323
TasI	AATT	7 cut(s)	300, 750, 917, 1031, 1038, 1155, 1225
TatI	WGTACW	3 cut(s)	177, 558, 1011
TauI	GCSGC	2 cut(s)	158, 161
TfiI	GAWTC	1 cut(s)	1304
Tru1I	TTAA	5 cut(s)	954, 1026, 1215, 1238, 1281
Tru9I	TTAA	5 cut(s)	954, 1026, 1215, 1238, 1281
TscAI	CASTG	3 cut(s)	259, 289, 370
TseFI	GTSAC	3 cut(s)	250, 329, 1138
TseI	GCWGC	4 cut(s)	636, 908, 1316, 1334
Tsp45I	GTSAC	3 cut(s)	250, 329, 1138
TspDTI	ATGAA	2 cut(s)	810, 1012
TspGWI	ACGGA	1 cut(s)	112
TspRI	CASTG	3 cut(s)	259, 289, 370
Van91I	CCANNNNNTGG	1 cut(s)	1261
VpaK11BI	GGWCC	1 cut(s)	395
XapI	RAATTY	3 cut(s)	300, 917, 1031
XceI	RCATGY	1 cut(s)	1185
XcmI	CCANNNNNNNNNTGG	1 cut(s)	499
XmiI	GTMKAC	1 cut(s)	342
XmnI	GAANNNNTTC	2 cut(s)	1167, 1308
XspI	CTAG	3 cut(s)	8, 839, 1416

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.