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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 124, 172 |
| AgsI | TTSAA | 2 cut(s) | 56, 83 |
| AleI | CACNNNNGTG | 1 cut(s) | 135 |
| AluBI | AGCT | 3 cut(s) | 208, 325, 364 |
| AluI | AGCT | 3 cut(s) | 208, 325, 364 |
| Alw21I | GWGCWC | 1 cut(s) | 175 |
| AlwI | GGATC | 2 cut(s) | 124, 172 |
| ApeKI | GCWGC | 1 cut(s) | 194 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 35, 78 |
| BanII | GRGCYC | 1 cut(s) | 130 |
| BauI | CACGAG | 1 cut(s) | 131 |
| Bbv12I | GWGCWC | 1 cut(s) | 175 |
| BbvI | GCAGC | 1 cut(s) | 181 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 122, 156 |
| BfaI | CTAG | 2 cut(s) | 144, 168 |
| BfmI | CTRYAG | 1 cut(s) | 159 |
| BisI | GCNGC | 1 cut(s) | 195 |
| BlsI | GCNGC | 1 cut(s) | 196 |
| BmsI | GCATC | 4 cut(s) | 33, 130, 181, 246 |
| BseXI | GCAGC | 1 cut(s) | 181 |
| BsiHKAI | GWGCWC | 1 cut(s) | 175 |
| Bsp1286I | GDGCHC | 2 cut(s) | 130, 175 |
| Bsp143I | GATC | 3 cut(s) | 116, 164, 385 |
| BspHI | TCATGA | 1 cut(s) | 27 |
| BspPI | GGATC | 2 cut(s) | 124, 172 |
| BssMI | GATC | 3 cut(s) | 116, 164, 385 |
| BssSI | CACGAG | 1 cut(s) | 131 |
| Bst2BI | CACGAG | 1 cut(s) | 131 |
| Bst4CI | ACNGT | 1 cut(s) | 263 |
| BstDEI | CTNAG | 1 cut(s) | 198 |
| BstKTI | GATC | 3 cut(s) | 119, 167, 388 |
| BstMBI | GATC | 3 cut(s) | 116, 164, 385 |
| BstSFI | CTRYAG | 1 cut(s) | 159 |
| BstV1I | GCAGC | 1 cut(s) | 181 |
| BstX2I | RGATCY | 2 cut(s) | 116, 164 |
| BstYI | RGATCY | 2 cut(s) | 116, 164 |
| BtsI | GCAGTG | 1 cut(s) | 118 |
| BtsIMutI | CAGTG | 2 cut(s) | 118, 268 |
| CciI | TCATGA | 1 cut(s) | 27 |
| CviAII | CATG | 2 cut(s) | 28, 296 |
| CviJI | RGCY | 7 cut(s) | 5, 34, 128, 208, 325, 364, 381 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 34, 128, 208, 325, 364, 381 |
| DdeI | CTNAG | 1 cut(s) | 198 |
| DpnI | GATC | 3 cut(s) | 118, 166, 387 |
| DpnII | GATC | 3 cut(s) | 116, 164, 385 |
| Eco24I | GRGCYC | 1 cut(s) | 130 |
| EcoT38I | GRGCYC | 1 cut(s) | 130 |
| FaeI | CATG | 2 cut(s) | 31, 299 |
| FaiI | YATR | 4 cut(s) | 29, 254, 297, 320 |
| FatI | CATG | 2 cut(s) | 27, 295 |
| Fnu4HI | GCNGC | 1 cut(s) | 195 |
| FriOI | GRGCYC | 1 cut(s) | 130 |
| Fsp4HI | GCNGC | 1 cut(s) | 195 |
| FspBI | CTAG | 2 cut(s) | 144, 168 |
| GluI | GCNGC | 1 cut(s) | 195 |
| Hin1II | CATG | 2 cut(s) | 31, 299 |
| HinfI | GANTC | 4 cut(s) | 52, 149, 292, 340 |
| Hpy188I | TCNGA | 1 cut(s) | 71 |
| Hpy188III | TCNNGA | 4 cut(s) | 28, 40, 105, 344 |
| Hpy99I | CGWCG | 1 cut(s) | 71 |
| HpyAV | CCTTC | 1 cut(s) | 71 |
| HpyCH4III | ACNGT | 1 cut(s) | 263 |
| HpyCH4V | TGCA | 3 cut(s) | 46, 259, 370 |
| HpyF3I | CTNAG | 1 cut(s) | 198 |
| Hsp92II | CATG | 2 cut(s) | 31, 299 |
| Kzo9I | GATC | 3 cut(s) | 116, 164, 385 |
| LpnPI | CCDG | 2 cut(s) | 53, 90 |
| Lsp1109I | GCAGC | 1 cut(s) | 181 |
| LweI | GCATC | 4 cut(s) | 33, 130, 181, 246 |
| MaeI | CTAG | 2 cut(s) | 144, 168 |
| MaeIII | GTNAC | 1 cut(s) | 146 |
| MalI | GATC | 3 cut(s) | 118, 166, 387 |
| MboI | GATC | 3 cut(s) | 116, 164, 385 |
| MboII | GAAGA | 1 cut(s) | 322 |
| MfeI | CAATTG | 2 cut(s) | 275, 374 |
| MflI | RGATCY | 2 cut(s) | 116, 164 |
| MhlI | GDGCHC | 2 cut(s) | 130, 175 |
| MluCI | AATT | 3 cut(s) | 275, 374, 397 |
| MlyI | GAGTC | 2 cut(s) | 143, 286 |
| MmeI | TCCRAC | 3 cut(s) | 49, 210, 219 |
| MnlI | CCTC | 1 cut(s) | 83 |
| MroXI | GAANNNNTTC | 2 cut(s) | 35, 78 |
| MseI | TTAA | 2 cut(s) | 282, 400 |
| MslI | CAYNNNNRTG | 1 cut(s) | 135 |
| MunI | CAATTG | 2 cut(s) | 275, 374 |
| NdeII | GATC | 3 cut(s) | 116, 164, 385 |
| NlaIII | CATG | 2 cut(s) | 31, 299 |
| NmuCI | GTSAC | 1 cut(s) | 146 |
| OliI | CACNNNNGTG | 1 cut(s) | 135 |
| PagI | TCATGA | 1 cut(s) | 27 |
| PdmI | GAANNNNTTC | 2 cut(s) | 35, 78 |
| PfeI | GAWTC | 2 cut(s) | 52, 340 |
| PkrI | GCNGC | 1 cut(s) | 196 |
| PleI | GAGTC | 2 cut(s) | 143, 286 |
| PpsI | GAGTC | 2 cut(s) | 143, 286 |
| PsuI | RGATCY | 2 cut(s) | 116, 164 |
| RseI | CAYNNNNRTG | 1 cut(s) | 135 |
| SaqAI | TTAA | 2 cut(s) | 282, 400 |
| SatI | GCNGC | 1 cut(s) | 195 |
| Sau3AI | GATC | 3 cut(s) | 116, 164, 385 |
| SchI | GAGTC | 2 cut(s) | 143, 286 |
| SduI | GDGCHC | 2 cut(s) | 130, 175 |
| SetI | ASST | 4 cut(s) | 210, 239, 327, 366 |
| SfaNI | GCATC | 4 cut(s) | 33, 130, 181, 246 |
| SfcI | CTRYAG | 1 cut(s) | 159 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 135 |
| Sse9I | AATT | 3 cut(s) | 275, 374, 397 |
| SspMI | CTAG | 2 cut(s) | 144, 168 |
| TaaI | ACNGT | 1 cut(s) | 263 |
| TaqI | TCGA | 1 cut(s) | 345 |
| TasI | AATT | 3 cut(s) | 275, 374, 397 |
| TfiI | GAWTC | 2 cut(s) | 52, 340 |
| Tru1I | TTAA | 2 cut(s) | 282, 400 |
| Tru9I | TTAA | 2 cut(s) | 282, 400 |
| TscAI | CASTG | 2 cut(s) | 118, 268 |
| TseFI | GTSAC | 1 cut(s) | 146 |
| TseI | GCWGC | 1 cut(s) | 194 |
| Tsp45I | GTSAC | 1 cut(s) | 146 |
| TspDTI | ATGAA | 3 cut(s) | 16, 44, 323 |
| TspRI | CASTG | 2 cut(s) | 118, 268 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 389 |
| XmnI | GAANNNNTTC | 2 cut(s) | 35, 78 |
| XspI | CTAG | 2 cut(s) | 144, 168 |