AT2G28230

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013016
Physical Location & Seq
Reverse (-)
12037627 .. 12039059
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28230.2

Sequence Viewer

Length: 660 bp
ATGCCCGTCAAGTGGGTTTTACATTGGCAACCAAACCAAGGATCAACAGTAAGCAGCCAAATCCTAAACGAAGCAACACAATGTGTAGAGAGCATCAATGGTGTGAAAGAAGGAAGATGGAAAGCAACACTCAACTATTACAAACCCATGTTAAAAGACCAAGCGAATCAATTGGAGTTTCCTCGTGATTTTCTAGGGATTTCACTTGCGGATCAGCCGAATAAGTACTATTTCATTATCAGGACGCAGAGGATTGTCTTGGAAGCTGATTCTTCGATTCAGTTGATTATGGAGAAGCTTCAGTCTTATAAATCTAAAGTGGCTCTTTACTTTGATGGGTTTCAGTATCAGCTTGGTGATTTTAGGTTGAGAGTTGGTAAAGTTGTTCCTACTCATTCTGAGAATGTTAGAGGCATTGTCATGGAGGTGGAGTATCTTCCTATATCATCAATGGAAAAGGCACAAAAGGTGATGGAGGAGTTCTTGGAGATATGGAATGAAGCTCTGGCTAAAAGGTCGTTGCCGGGTAAGTTTGTGAACATAGATCTCAACTTTGGGGAGTTTGGACTTGGAGACATCTACACTCCACAACACACAGCTGTTCGTTACGCTCTCGTGATGGCTCACATGATTGCTACCGTTCAAGCTGTGAGAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002831 GO:0003674 GO:0003712 GO:0003713 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0031047 GO:0031050 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043900 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0097659 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902680 GO:1903506 GO:1903508 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med20

Protein Analysis

219

Amino Acids

25.13

Weight (kDa)

7.79

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med20 PF08612 6 - 158 2e-39 TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28230 AT2G28230 AT4G09070
fragaria_vesca FvH4_1g08420
malus_domestica MD15G1215700.v1.1 MD15G1215800.v1.1
prunus_persica Prupe.7G201200_v2.0.a1
pyrus_communis pycom02g07100 pycom15g19140
rosa_chinensis RchiOBHm_Chr2g0094521
rosa_laevigata RLG00000016498
rosa_multiflora Rmu_sc0004359.1_g000043
rosa_roxburghii Rroxscaffold_2G00147100
rosa_rugosa Rorug02G0044400
rosa_samantha Rh2AG092700 Rh2BG092900 Rh2CG095400 Rh2DG091800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
AciI CCGC 1 cut(s) 209
AclWI GGATC 2 cut(s) 49, 219
AcuI CTGAAG 1 cut(s) 284
AdeI CACNNNGTG 1 cut(s) 83
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 12, 38
AgsI TTSAA 1 cut(s) 644
AluBI AGCT 6 cut(s) 266, 298, 352, 503, 599, 647
AluI AGCT 6 cut(s) 266, 298, 352, 503, 599, 647
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 2 cut(s) 49, 219
ApeKI GCWGC 1 cut(s) 54
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 368, 481
BauI CACGAG 2 cut(s) 183, 614
BbvI GCAGC 1 cut(s) 66
BccI CCATC 4 cut(s) 111, 329, 466, 613
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 1 cut(s) 194
BglII AGATCT 1 cut(s) 544
BisI GCNGC 1 cut(s) 55
BlsI GCNGC 1 cut(s) 56
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 525
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 1 cut(s) 102
BpuMI CCSGG 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 38
BseDI CCNNGG 1 cut(s) 37
BseLI CCNNNNNNNGG 2 cut(s) 12, 38
BseMII CTCAG 1 cut(s) 390
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 524
BslI CCNNNNNNNGG 2 cut(s) 12, 38
BsmAI GTCTC 1 cut(s) 567
Bsp143I GATC 3 cut(s) 41, 211, 544
BspACI CCGC 1 cut(s) 209
BspCNI CTCAG 1 cut(s) 391
BspPI GGATC 2 cut(s) 49, 219
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 3 cut(s) 41, 211, 544
BssSI CACGAG 2 cut(s) 183, 614
BssT1I CCWWGG 1 cut(s) 37
Bst2BI CACGAG 2 cut(s) 183, 614
Bst4CI ACNGT 2 cut(s) 49, 640
BstDEI CTNAG 1 cut(s) 399
BstKTI GATC 3 cut(s) 44, 214, 547
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 3 cut(s) 41, 211, 544
BstSCI CCNGG 1 cut(s) 523
BstV1I GCAGC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 544
BstYI RGATCY 1 cut(s) 544
CseI GACGC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 226
CviAII CATG 3 cut(s) 148, 421, 628
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 399
DpnI GATC 3 cut(s) 43, 213, 546
DpnII GATC 3 cut(s) 41, 211, 544
DraIII CACNNNGTG 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 284
EcoT14I CCWWGG 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 3 cut(s) 151, 424, 631
FaiI YATR 8 cut(s) 149, 290, 309, 422, 443, 493, 542, 629
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 3 cut(s) 147, 420, 627
Fnu4HI GCNGC 1 cut(s) 55
Fsp4HI GCNGC 1 cut(s) 55
FspBI CTAG 1 cut(s) 194
GluI GCNGC 1 cut(s) 55
HapII CCGG 1 cut(s) 524
HgaI GACGC 1 cut(s) 253
Hin1II CATG 3 cut(s) 151, 424, 631
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 3 cut(s) 166, 269, 277
HpaII CCGG 1 cut(s) 524
HphI GGTGA 2 cut(s) 368, 481
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 1 cut(s) 400
Hpy188III TCNNGA 3 cut(s) 185, 241, 616
Hpy8I GTNNAC 1 cut(s) 538
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 2 cut(s) 49, 640
HpyF3I CTNAG 1 cut(s) 399
Hsp92II CATG 3 cut(s) 151, 424, 631
Kzo9I GATC 3 cut(s) 41, 211, 544
LpnPI CCDG 3 cut(s) 226, 491, 537
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 1 cut(s) 102
MaeI CTAG 1 cut(s) 194
MaeIII GTNAC 1 cut(s) 605
MalI GATC 3 cut(s) 43, 213, 546
MboI GATC 3 cut(s) 41, 211, 544
MboII GAAGA 3 cut(s) 126, 264, 428
MfeI CAATTG 1 cut(s) 170
MflI RGATCY 1 cut(s) 544
MluCI AATT 1 cut(s) 170
MnlI CCTC 6 cut(s) 192, 243, 404, 418, 469, 647
MseI TTAA 1 cut(s) 152
MslI CAYNNNNRTG 2 cut(s) 419, 425
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 1 cut(s) 525
MunI CAATTG 1 cut(s) 170
NciI CCSGG 1 cut(s) 525
NdeII GATC 3 cut(s) 41, 211, 544
NlaIII CATG 3 cut(s) 151, 424, 631
PfeI GAWTC 3 cut(s) 166, 269, 277
PkrI GCNGC 1 cut(s) 56
PsiI TTATAA 1 cut(s) 309
PsuI RGATCY 1 cut(s) 544
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 2 cut(s) 419, 425
SaqAI TTAA 1 cut(s) 152
SatI GCNGC 1 cut(s) 55
Sau3AI GATC 3 cut(s) 41, 211, 544
ScaI AGTACT 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 525
SfaNI GCATC 1 cut(s) 102
SmiMI CAYNNNNRTG 2 cut(s) 419, 425
Sse9I AATT 1 cut(s) 170
SsiI CCGC 1 cut(s) 209
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 523
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 2 cut(s) 49, 640
TaqI TCGA 1 cut(s) 275
TasI AATT 1 cut(s) 170
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 3 cut(s) 166, 269, 277
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TseI GCWGC 1 cut(s) 54
TspDTI ATGAA 2 cut(s) 223, 513
XspI CTAG 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.