MD15G1215800.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013016
Physical Location & Seq
Reverse (-)
17423397 .. 17425453
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1215800.v1.1.491

Sequence Viewer

Length: 789 bp
ATGCTTTGCATCCCAATTTATTTTACTTCTCTTATCTCACTAAAACCCTTCATCTCCTCCGTCAGCGTCCTACATCGACTGCAAAATCCTAATCAGAAACCTCCGATATCCGAGGCAATGCCGCTCAAATGGGTTTTGCATTGGCAGCCCAACGCAGGGACGACGGTGAACAGTCAGATCATAAACGAAGTCACGCAGTGCGTCGAGAGCATCAATGGCGTCAAGGAAGGCAGGTGGAAAGCCACCATCACCTTCTACAAACCCATACTGCGAGAGCCCTCTTTAACGGGGGAGCCCACGCGTGATTTTCTGGGGATTGCGCTGCCGGAGCAACCCAGTAAGTACTACCTGATAATGCGGTTGCAGCGGATAATTCTCGAGGCGGATTCATCAATTCAGACGATTATGGAGAAGTTACAGTCTTACAAATCAAGGGTCTCGCTTTATTTTGAGGGGTTTCATTATCAACTCGGGGACTTCCAATTGAGAGTGGGGAAAGTTATTCCTACTCATTTGGAAAATTTGAGAGGAATAGTTATGGAGCTGGAGTACCTTCCCATTTCTTCGATGGAAAAAGCGAGGCAAGTCATGGAAGAGTTCATGGAGATTTGGCAACAGGCTTTGGCGAAAAGATCATTACCAGGACACTTCATGCATGTGGAACCAAACTTTTCCGAGTATGGCCTTGGAGACCAATATACTTCACAACATACTGCTGTCCAGTATGCTGCCATTATGGCTCAACTCGTTGCAACCGTTCAAGCAGTGCAGCAATCGGCAAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002831 GO:0003674 GO:0003712 GO:0003713 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0031047 GO:0031050 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043900 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0097659 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902680 GO:1903506 GO:1903508 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med20

Protein Analysis

263

Amino Acids

30.0

Weight (kDa)

7.79

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med20 PF08612 40 - 246 1.6e-53 TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28230 AT2G28230 AT4G09070
fragaria_vesca FvH4_1g08420
malus_domestica MD15G1215700.v1.1 MD15G1215800.v1.1
prunus_persica Prupe.7G201200_v2.0.a1
pyrus_communis pycom02g07100 pycom15g19140
rosa_chinensis RchiOBHm_Chr2g0094521
rosa_laevigata RLG00000016498
rosa_multiflora Rmu_sc0004359.1_g000043
rosa_roxburghii Rroxscaffold_2G00147100
rosa_rugosa Rorug02G0044400
rosa_samantha Rh2AG092700 Rh2BG092900 Rh2CG095400 Rh2DG091800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 222
Acc36I ACCTGC 1 cut(s) 222
AccBSI CCGCTC 1 cut(s) 124
AccII CGCG 1 cut(s) 301
AciI CCGC 4 cut(s) 122, 358, 367, 383
AcsI RAATTY 1 cut(s) 520
AcyI GRCGYC 1 cut(s) 219
AdeI CACNNNGTG 1 cut(s) 198
AfaI GTAC 2 cut(s) 344, 551
AfiI CCNNNNNNNGG 2 cut(s) 155, 156
AflIII ACRYGT 1 cut(s) 299
AgsI TTSAA 1 cut(s) 761
AjnI CCWGG 1 cut(s) 640
AluBI AGCT 1 cut(s) 544
AluI AGCT 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 442, 684
Ama87I CYCGRG 2 cut(s) 377, 470
AoxI GGCC 1 cut(s) 682
ApeKI GCWGC 5 cut(s) 145, 322, 364, 728, 769
ApoI RAATTY 1 cut(s) 520
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AspLEI GCGC 1 cut(s) 322
AsuHPI GGTGA 2 cut(s) 178, 241
AvaI CYCGRG 2 cut(s) 377, 470
BanII GRGCYC 2 cut(s) 279, 297
BbvI GCAGC 5 cut(s) 157, 309, 376, 715, 781
BccI CCATC 2 cut(s) 254, 562
BciT130I CCWGG 1 cut(s) 642
BcoDI GTCTC 2 cut(s) 442, 684
BfuAI ACCTGC 1 cut(s) 222
BglI GCCNNNNNGGC 1 cut(s) 737
BisI GCNGC 6 cut(s) 122, 146, 323, 365, 729, 770
BlsI GCNGC 6 cut(s) 123, 147, 324, 366, 730, 771
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 1 cut(s) 642
BmeT110I CYCGRG 2 cut(s) 377, 470
BmiI GGNNCC 2 cut(s) 294, 663
BmrFI CCNGG 1 cut(s) 642
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 18, 219
BmuI ACTGGG 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 2 cut(s) 442, 684
BsaJI CCNNGG 2 cut(s) 111, 685
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 156
Bse1I ACTGG 2 cut(s) 336, 721
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 2 cut(s) 111, 685
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 2 cut(s) 155, 156
BseMI GCAATG 1 cut(s) 123
BseNI ACTGG 2 cut(s) 336, 721
BseRI GAGGAG 1 cut(s) 46
BseXI GCAGC 5 cut(s) 157, 309, 376, 715, 781
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 301
BshFI GGCC 1 cut(s) 684
BsiHKCI CYCGRG 2 cut(s) 377, 470
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 2 cut(s) 172, 488
BslI CCNNNNNNNGG 2 cut(s) 155, 156
BsmAI GTCTC 2 cut(s) 442, 684
BsmFI GGGAC 2 cut(s) 172, 488
BsnI GGCC 1 cut(s) 684
Bso31I GGTCTC 2 cut(s) 442, 684
BsoBI CYCGRG 2 cut(s) 377, 470
Bsp1286I GDGCHC 2 cut(s) 279, 297
Bsp143I GATC 2 cut(s) 177, 632
BspACI CCGC 4 cut(s) 122, 358, 367, 383
BspANI GGCC 1 cut(s) 684
BspFNI CGCG 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 294, 663
BspMI ACCTGC 1 cut(s) 222
BspTNI GGTCTC 2 cut(s) 442, 684
BsrBI CCGCTC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 123
BsrI ACTGG 2 cut(s) 336, 721
BssECI CCNNGG 2 cut(s) 111, 685
BssMI GATC 2 cut(s) 177, 632
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 685
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 4 cut(s) 166, 173, 420, 757
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 219
BstF5I GGATG 1 cut(s) 9
BstFNI CGCG 1 cut(s) 301
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 2 cut(s) 180, 635
BstMAI GTCTC 2 cut(s) 442, 684
BstMBI GATC 2 cut(s) 177, 632
BstMWI GCNNNNNNNGC 6 cut(s) 145, 207, 216, 328, 364, 737
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 1 cut(s) 640
BstUI CGCG 1 cut(s) 301
BstV1I GCAGC 5 cut(s) 157, 309, 376, 715, 781
BsuRI GGCC 1 cut(s) 684
BtsCI GGATG 1 cut(s) 9
BtsI GCAGTG 2 cut(s) 203, 771
BtsIMutI CAGTG 2 cut(s) 203, 771
BveI ACCTGC 1 cut(s) 222
CfoI GCGC 1 cut(s) 322
CseI GACGC 3 cut(s) 55, 190, 208
Csp6I GTAC 2 cut(s) 343, 550
CviAII CATG 4 cut(s) 589, 601, 652, 656
CviJI RGCY 8 cut(s) 148, 242, 277, 295, 544, 620, 684, 740
CviKI_1 RGCY 8 cut(s) 148, 242, 277, 295, 544, 620, 684, 740
CviQI GTAC 2 cut(s) 343, 550
DpnI GATC 2 cut(s) 179, 634
DpnII GATC 2 cut(s) 177, 632
DraIII CACNNNGTG 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
EciI GGCGGA 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 685
Eco24I GRGCYC 2 cut(s) 279, 297
Eco31I GGTCTC 2 cut(s) 442, 684
Eco32I GATATC 1 cut(s) 108
Eco88I CYCGRG 2 cut(s) 377, 470
EcoRII CCWGG 1 cut(s) 640
EcoRV GATATC 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 685
EcoT22I ATGCAT 1 cut(s) 657
EcoT38I GRGCYC 2 cut(s) 279, 297
ErhI CCWWGG 1 cut(s) 685
FaeI CATG 4 cut(s) 592, 604, 655, 659
FaqI GGGAC 2 cut(s) 172, 488
FatI CATG 4 cut(s) 588, 600, 651, 655
Fnu4HI GCNGC 6 cut(s) 122, 146, 323, 365, 729, 770
FriOI GRGCYC 2 cut(s) 279, 297
Fsp4HI GCNGC 6 cut(s) 122, 146, 323, 365, 729, 770
GlaI GCGC 1 cut(s) 321
GluI GCNGC 6 cut(s) 122, 146, 323, 365, 729, 770
GsuI CTGGAG 1 cut(s) 566
HaeIII GGCC 1 cut(s) 684
HapII CCGG 1 cut(s) 326
HgaI GACGC 3 cut(s) 55, 190, 208
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 592, 604, 655, 659
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HinfI GANTC 1 cut(s) 386
HpaII CCGG 1 cut(s) 326
HphI GGTGA 2 cut(s) 178, 241
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 6 cut(s) 96, 105, 112, 177, 399, 676
Hpy188III TCNNGA 2 cut(s) 205, 377
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 2 cut(s) 166, 206
HpyAV CCTTC 4 cut(s) 58, 221, 262, 563
HpyCH4III ACNGT 4 cut(s) 166, 173, 420, 757
HpyCH4V TGCA 7 cut(s) 9, 82, 139, 364, 655, 752, 769
HpyF10VI GCNNNNNNNGC 6 cut(s) 145, 207, 216, 328, 364, 737
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 592, 604, 655, 659
HspAI GCGC 1 cut(s) 320
Kzo9I GATC 2 cut(s) 177, 632
LmnI GCTCC 3 cut(s) 292, 328, 541
Lsp1109I GCAGC 5 cut(s) 157, 309, 376, 715, 781
LweI GCATC 2 cut(s) 18, 219
MaeIII GTNAC 2 cut(s) 190, 414
MalI GATC 2 cut(s) 179, 634
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 177, 632
MboII GAAGA 2 cut(s) 555, 605
MfeI CAATTG 1 cut(s) 482
MhlI GDGCHC 2 cut(s) 279, 297
MluCI AATT 6 cut(s) 15, 372, 393, 482, 520, 784
MluI ACGCGT 1 cut(s) 299
MnlI CCTC 8 cut(s) 67, 106, 111, 289, 373, 445, 521, 573
Mph1103I ATGCAT 1 cut(s) 657
MseI TTAA 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 656
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 1 cut(s) 642
MunI CAATTG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 642
MvnI CGCG 1 cut(s) 301
MwoI GCNNNNNNNGC 6 cut(s) 145, 207, 216, 328, 364, 737
NdeII GATC 2 cut(s) 177, 632
NlaIII CATG 4 cut(s) 592, 604, 655, 659
NlaIV GGNNCC 2 cut(s) 294, 663
NmuCI GTSAC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 657
NspI RCATGY 1 cut(s) 659
PaeR7I CTCGAG 1 cut(s) 377
PaqCI CACCTGC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 753
PfeI GAWTC 1 cut(s) 386
PkrI GCNGC 6 cut(s) 123, 147, 324, 366, 730, 771
Psp6I CCWGG 1 cut(s) 640
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 2 cut(s) 294, 663
RsaI GTAC 2 cut(s) 344, 551
RsaNI GTAC 2 cut(s) 343, 550
RseI CAYNNNNRTG 1 cut(s) 656
SaqAI TTAA 1 cut(s) 284
SatI GCNGC 6 cut(s) 122, 146, 323, 365, 729, 770
Sau3AI GATC 2 cut(s) 177, 632
ScaI AGTACT 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 642
SduI GDGCHC 2 cut(s) 279, 297
SetI ASST 6 cut(s) 103, 236, 254, 351, 546, 555
SfaNI GCATC 2 cut(s) 18, 219
Sfr274I CTCGAG 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 6 cut(s) 15, 372, 393, 482, 520, 784
SsiI CCGC 4 cut(s) 122, 358, 367, 383
StyD4I CCNGG 1 cut(s) 640
StyI CCWWGG 1 cut(s) 685
TaaI ACNGT 4 cut(s) 166, 173, 420, 757
TaqI TCGA 4 cut(s) 76, 204, 378, 566
TasI AATT 6 cut(s) 15, 372, 393, 482, 520, 784
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TscAI CASTG 2 cut(s) 203, 771
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 5 cut(s) 145, 322, 364, 728, 769
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 5 cut(s) 40, 378, 449, 589, 640
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 2 cut(s) 203, 771
XapI RAATTY 1 cut(s) 520
XceI RCATGY 1 cut(s) 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 565
XhoI CTCGAG 1 cut(s) 377
ZrmI AGTACT 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.