pycom02g07100

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0013016
Physical Location & Seq
Reverse (-)
4957822 .. 4959057
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g07100.1

Sequence Viewer

Length: 561 bp
ATGGCGTCAAGGAAGGAAGAGCCGTCTTTAACCGGGGAACCCCCGCGTTATCTTCTGGGGATTGCGCTGCCGGAGCAACCCAGTAAGTACTACCTGATAATGCGGTTGCAGCGGATAATTCTCGAGGTGGATTCTTCGATTCAGGCGATTATGGAGAAGTTACAGTCGTACAAATCGAGGGTCTCGCTTTATTTTGAGGTTTCCCCCTCCAATTGGTGTAGTTGGAAGGGGTTTCAGTATCAAGTCGGGGACTTCCAATTGAGAGTGGGGAAAGTTATTCCTACTCATTCCGAAAATTTAAGAGGAATAGTTATGGAACTGGAGTACCTTCCCATTTCTTCAATGGAAAAAGCGAGGCAAGTCATGGAAGAGTTCATGGATATATGGCAGCAGGCTTTGTCGAAAAGATCATTACTGGGGCACTTCATGCACGTGGAACCAAACTTTTCCAAGTATGGCCTTGACCAATATACTTCACAACATACTGCTGTCCAGTATGCTGCCATTATGGCTCAACTCATTGCGACCGTGCAAGCAGTGCAGCAATCAGCAAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002831 GO:0003674 GO:0003712 GO:0003713 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016592 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0031047 GO:0031050 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031974 GO:0031981 GO:0032101 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043900 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0097659 GO:0140110 GO:1900150 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902680 GO:1903506 GO:1903508 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med20

Protein Analysis

187

Amino Acids

21.47

Weight (kDa)

7.83

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med20 PF08612 12 - 170 1.6e-30 TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28230 AT2G28230 AT4G09070
fragaria_vesca FvH4_1g08420
malus_domestica MD15G1215700.v1.1 MD15G1215800.v1.1
prunus_persica Prupe.7G201200_v2.0.a1
pyrus_communis pycom02g07100 pycom15g19140
rosa_chinensis RchiOBHm_Chr2g0094521
rosa_laevigata RLG00000016498
rosa_multiflora Rmu_sc0004359.1_g000043
rosa_roxburghii Rroxscaffold_2G00147100
rosa_rugosa Rorug02G0044400
rosa_samantha Rh2AG092700 Rh2BG092900 Rh2CG095400 Rh2DG091800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 46
AciI CCGC 3 cut(s) 44, 103, 112
AcsI RAATTY 1 cut(s) 295
AcvI CACGTG 1 cut(s) 433
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 89, 170, 326
AfiI CCNNNNNNNGG 1 cut(s) 213
AgsI TTSAA 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 1 cut(s) 457
ApeKI GCWGC 5 cut(s) 67, 109, 388, 500, 541
ApoI RAATTY 1 cut(s) 295
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AspLEI GCGC 1 cut(s) 67
AsuC2I CCSGG 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 423
BaeI ACNNNNGTAYC 2 cut(s) 308, 341
BbrPI CACGTG 1 cut(s) 433
BbvI GCAGC 5 cut(s) 54, 121, 400, 487, 553
BceAI ACGGC 1 cut(s) 7
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 509
BisI GCNGC 5 cut(s) 68, 110, 389, 501, 542
BlsI GCNGC 5 cut(s) 69, 111, 390, 502, 543
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 122
BmiI GGNNCC 2 cut(s) 39, 438
BmrFI CCNGG 1 cut(s) 34
BmrI ACTGGG 2 cut(s) 75, 425
BmuI ACTGGG 2 cut(s) 75, 425
BpmI CTGGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 34
BsaAI YACGTR 1 cut(s) 433
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 213
Bse1I ACTGG 4 cut(s) 81, 324, 420, 493
Bse3DI GCAATG 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 213
BseMI GCAATG 1 cut(s) 519
BseNI ACTGG 4 cut(s) 81, 324, 420, 493
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 5 cut(s) 54, 121, 400, 487, 553
BsgI GTGCAG 1 cut(s) 560
Bsh1236I CGCG 1 cut(s) 46
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 528
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 2 cut(s) 33, 71
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 213
BsmAI GTCTC 1 cut(s) 187
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 459
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 1 cut(s) 407
BspACI CCGC 3 cut(s) 44, 103, 112
BspANI GGCC 1 cut(s) 459
BspFNI CGCG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 39, 438
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 4 cut(s) 81, 324, 420, 493
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 1 cut(s) 407
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 2 cut(s) 165, 529
Bst6I CTCTTC 2 cut(s) 12, 363
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 427, 538
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 2 cut(s) 393, 534
BstFNI CGCG 1 cut(s) 46
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 1 cut(s) 410
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 1 cut(s) 407
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 5 cut(s) 73, 109, 427, 509, 538
BstSCI CCNGG 1 cut(s) 32
BstSLI GKGCMC 1 cut(s) 423
BstUI CGCG 1 cut(s) 46
BstV1I GCAGC 5 cut(s) 54, 121, 400, 487, 553
BsuRI GGCC 1 cut(s) 459
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 543
Cac8I GCNNGC 2 cut(s) 393, 534
CfoI GCGC 1 cut(s) 67
Csp6I GTAC 3 cut(s) 88, 169, 325
CviAII CATG 3 cut(s) 364, 376, 427
CviJI RGCY 4 cut(s) 22, 395, 459, 512
CviKI_1 RGCY 4 cut(s) 22, 395, 459, 512
CviQI GTAC 3 cut(s) 88, 169, 325
DpnI GATC 1 cut(s) 409
DpnII GATC 1 cut(s) 407
Eam1104I CTCTTC 2 cut(s) 12, 363
EarI CTCTTC 2 cut(s) 12, 363
Eco31I GGTCTC 1 cut(s) 187
Eco72I CACGTG 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 122
FaeI CATG 3 cut(s) 367, 379, 430
FaqI GGGAC 1 cut(s) 263
FatI CATG 3 cut(s) 363, 375, 426
FauI CCCGC 1 cut(s) 51
Fnu4HI GCNGC 5 cut(s) 68, 110, 389, 501, 542
Fsp4HI GCNGC 5 cut(s) 68, 110, 389, 501, 542
GlaI GCGC 1 cut(s) 66
GluI GCNGC 5 cut(s) 68, 110, 389, 501, 542
GsuI CTGGAG 1 cut(s) 341
HaeIII GGCC 1 cut(s) 459
HapII CCGG 2 cut(s) 33, 71
HhaI GCGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 367, 379, 430
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 2 cut(s) 131, 139
HpaII CCGG 2 cut(s) 33, 71
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 7, 220, 338
HpyCH4III ACNGT 2 cut(s) 165, 529
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 4 cut(s) 109, 430, 532, 541
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 109, 427, 509, 538
HpySE526I ACGT 1 cut(s) 432
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 367, 379, 430
HspAI GCGC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 407
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 5 cut(s) 54, 121, 400, 487, 553
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 159
MalI GATC 1 cut(s) 409
MboI GATC 1 cut(s) 407
MboII GAAGA 5 cut(s) 29, 44, 126, 330, 380
MfeI CAATTG 2 cut(s) 211, 257
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 5 cut(s) 117, 211, 257, 295, 556
MmeI TCCRAC 1 cut(s) 203
MnlI CCTC 6 cut(s) 118, 171, 190, 217, 296, 348
MseI TTAA 2 cut(s) 29, 299
MslI CAYNNNNRTG 1 cut(s) 431
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 2 cut(s) 33, 71
MspR9I CCNGG 1 cut(s) 34
MunI CAATTG 2 cut(s) 211, 257
MvnI CGCG 1 cut(s) 46
MwoI GCNNNNNNNGC 5 cut(s) 73, 109, 427, 509, 538
NciI CCSGG 1 cut(s) 34
NdeII GATC 1 cut(s) 407
NlaIII CATG 3 cut(s) 367, 379, 430
NlaIV GGNNCC 2 cut(s) 39, 438
PaeR7I CTCGAG 1 cut(s) 122
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 173
PfeI GAWTC 2 cut(s) 131, 139
PkrI GCNGC 5 cut(s) 69, 111, 390, 502, 543
PmaCI CACGTG 1 cut(s) 433
PmlI CACGTG 1 cut(s) 433
Ppu21I YACGTR 1 cut(s) 433
PspCI CACGTG 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 39, 438
RsaI GTAC 3 cut(s) 89, 170, 326
RsaNI GTAC 3 cut(s) 88, 169, 325
RseI CAYNNNNRTG 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 29, 299
SatI GCNGC 5 cut(s) 68, 110, 389, 501, 542
Sau3AI GATC 1 cut(s) 407
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 1 cut(s) 423
SetI ASST 5 cut(s) 96, 129, 201, 330, 435
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 5 cut(s) 117, 211, 257, 295, 556
SsiI CCGC 3 cut(s) 44, 103, 112
StyD4I CCNGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 165, 529
TaiI ACGT 1 cut(s) 435
TaqI TCGA 4 cut(s) 123, 137, 176, 401
TasI AATT 5 cut(s) 117, 211, 257, 295, 556
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 2 cut(s) 131, 139
Tru1I TTAA 2 cut(s) 29, 299
Tru9I TTAA 2 cut(s) 29, 299
TscAI CASTG 1 cut(s) 543
TseI GCWGC 5 cut(s) 67, 109, 388, 500, 541
TspDTI ATGAA 2 cut(s) 364, 415
TspRI CASTG 1 cut(s) 543
XapI RAATTY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 122
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.