AT2G32750

macromolecule glycosylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013818
Physical Location & Seq
Forward (+)
13889573 .. 13891269
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32750.1

Sequence Viewer

Length: 1530 bp
ATGATGAAACCAGTTCCAAAGCTTTGGGTTGTCATCTCAAGTGCCTTTGTGTTCTGTCTTTTAGTTCTGTTTCAAATCAATAAATCAGATTTGATCGAAGCAAATCTCCAAATCACTCATCAAGTTAACAACTTCCTCATCTCCTTCGTTGCTTCTTCAAATAATCAAATCCTAAATCACACCAAACATGCGAATGAGAGTGATGGAATCAGAGCGAAACAGCTTGAGGAAGAAGAAACGGATACTTGCGCCGGAAGATACATCTACATGTACAATCTTCCAAGCACATTCAACGACGACATCATCAAAGAATGTCGTCCACTCATCAAATGGTTCGATATGTGTCCATTCATGGTCAATTCCGGGCTCGGTCCACAGATTTTGGTCTCCGATAAAACCACAGCTCGAGTCTTAACCGTGAAAACCGGTTCTTGGTACTCAACGAACCAATTCTTGCTTTCGGTTATCTTCCGAGAGAGGATGAAACACTACGAGTGTTTGACCAACAACTCTTCTCTAGCCTCAGCGATCTATGTCCCATACTACGCAGGATTTGATGTGAGCCGTCACCTCTGGGGATACAACGTGACGGTTAGAGACGAATTGGCGATAAAGCTGGCACAATGGCTGAGAGAGAGACCTGAATGGGGGAAGATGTACGGCCGAGATCACTTCTTTGTGACCGGACGGATTGGTTGGGATTTCAGGAGGTTTCACGATGAAGATTCAGATTGGGGAAGCAAACTGATGCTATTACCTGAATTCTCGAATCTGACAATGTTAGGTATCGAAACGACTGCTTGGGCTAACGAATTTGCGATTCCTTATCCTACTTATTTCCATCCAAAGAGCTTAACAGAGATTTGGAGATGGCAGAAGAAAGTGAAGAGTGTGAAGAGGAAGTATTTGTTTTCGTTTGTTGGAGGTCCAAGACCTAAATTGGATGGATCTATTAGGGGAGAGATTATAAAGCAGTGCCTTGCATCTCACGGTAAATGCAACTTTCTTAACTGCTTTGTTAATGACTGCGATAATCCGGTTAAGATAATGAAGGTGTTTGAAAATTCTGTCTTCTGTTTACAACCTTCGGGAGATTCGTACACTCGGAGATCGATATTCGATTCGATTCTGGCGGGTTGTATACCGGTTTTCTTCAGCCCAGGTTCGGGTTACAACCAATATATTTGGTATTTTCCAAAGGATTATACCAAGTACTCGGTTTACATACCAGAGAATGAAATGAGAAATGGAACGGTTAGTCTCAAGAATATTTTGGGTATGATCGCTAAGGAGAGGATTTTGAGGATGAGAAAAGAGGTTGTGAAGATTATACCAAAAATCATATATAATAAACCAGGGTTTGGACCGGAGAAGATTGAAGATGCGTTTGACATTGCAGTGGATAGGATGCTTGAGAGGGTAGCAATGGTTAAGAGGATGATGGAAGAAGGGAAAGATGTTCAAAGTCAGTATTATTCACAGACAAAGGATTTGAAAAAACTTGAGATTATACATGAAAAAACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exostosin

Protein Analysis

509

Amino Acids

59.13

Weight (kDa)

8.93

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 83 - 419 2e-80 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013818)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32750 AT2G32750
fragaria_vesca FvH4_5g28240
malus_domestica MD08G1246000.v1.1 MD15G1438500.v1.1
prunus_persica Prupe.1G581600_v2.0.a1
pyrus_communis pycom08g21350
rosa_chinensis RchiOBHm_Chr7g0220401
rosa_laevigata RLG00000002222
rosa_multiflora Rmu_sc0006673.1_g000004
rosa_roxburghii Rroxscaffold_3G00239410
rosa_rugosa Rorug07G0193700
rosa_samantha Rh7AG334800 Rh7BG325700 Rh7CG352500 Rh7DG331700
rosa_wichuraiana Rw7G028540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 968
AccB7I CCANNNNNTGG 1 cut(s) 1361
AccI GTMKAC 1 cut(s) 1141
AciI CCGC 1 cut(s) 1133
AclWI GGATC 1 cut(s) 955
AcoI YGGCCR 1 cut(s) 661
AcsI RAATTY 3 cut(s) 761, 812, 1063
AcuI CTGAAG 1 cut(s) 1138
AfaI GTAC 5 cut(s) 272, 437, 659, 1100, 1214
AfiI CCNNNNNNNGG 5 cut(s) 432, 647, 1165, 1166, 1361
AflIII ACRYGT 1 cut(s) 267
AgeI ACCGGT 2 cut(s) 425, 1144
AgsI TTSAA 7 cut(s) 74, 159, 292, 1061, 1379, 1463, 1495
AjnI CCWGG 2 cut(s) 1159, 1354
AluBI AGCT 5 cut(s) 22, 223, 404, 616, 852
AluI AGCT 5 cut(s) 22, 223, 404, 616, 852
Alw26I GTCTC 4 cut(s) 391, 591, 631, 1265
AlwI GGATC 1 cut(s) 955
Ama87I CYCGRG 1 cut(s) 405
AoxI GGCC 1 cut(s) 661
ApoI RAATTY 3 cut(s) 761, 812, 1063
ArsI GACNNNNNNTTYG 2 cut(s) 1474, 1506
AsiGI ACCGGT 2 cut(s) 425, 1144
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 3 cut(s) 371, 926, 1364
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 405
AvaII GGWCC 3 cut(s) 371, 926, 1364
BanII GRGCYC 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 1063
BbvCI CCTCAGC 1 cut(s) 523
BccI CCATC 5 cut(s) 197, 849, 864, 938, 1435
BceAI ACGGC 2 cut(s) 549, 676
BcgI CGANNNNNNTGC 2 cut(s) 779, 813
BciT130I CCWGG 2 cut(s) 1161, 1356
BciVI GTATCC 2 cut(s) 235, 572
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 4 cut(s) 391, 591, 631, 1265
BfaI CTAG 1 cut(s) 518
BfuI GTATCC 2 cut(s) 235, 572
BmcAI AGTACT 1 cut(s) 1214
Bme1390I CCNGG 3 cut(s) 364, 1161, 1356
Bme18I GGWCC 3 cut(s) 371, 926, 1364
BmeT110I CYCGRG 1 cut(s) 405
BmgT120I GGNCC 3 cut(s) 371, 926, 1364
BmrFI CCNGG 3 cut(s) 364, 1161, 1356
BmsI GCATC 4 cut(s) 738, 992, 1372, 1398
BpiI GAAGAC 1 cut(s) 1063
Bpu10I CCTNAGC 2 cut(s) 523, 1287
BpuEI CTTGAG 5 cut(s) 22, 245, 1247, 1433, 1523
BpuMI CCSGG 1 cut(s) 364
Bsa29I ATCGAT 1 cut(s) 1112
BsaI GGTCTC 2 cut(s) 391, 631
BsaJI CCNNGG 2 cut(s) 1159, 1355
BsaWI WCCGGW 5 cut(s) 425, 683, 1036, 1144, 1366
Bsc4I CCNNNNNNNGG 5 cut(s) 432, 647, 1165, 1166, 1361
Bse118I RCCGGY 2 cut(s) 425, 1144
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 2 cut(s) 1392, 1431
BseBI CCWGG 2 cut(s) 1161, 1356
BseCI ATCGAT 1 cut(s) 1112
BseDI CCNNGG 2 cut(s) 1159, 1355
BseGI GGATG 6 cut(s) 486, 841, 949, 1311, 1413, 1443
BseLI CCNNNNNNNGG 5 cut(s) 432, 647, 1165, 1166, 1361
BseMI GCAATG 2 cut(s) 1392, 1431
BseMII CTCAG 2 cut(s) 537, 620
BseNI ACTGG 1 cut(s) 11
BseX3I CGGCCG 1 cut(s) 661
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 1 cut(s) 663
BshTI ACCGGT 2 cut(s) 425, 1144
BshVI ATCGAT 1 cut(s) 1112
BsiEI CGRYCG 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 405
BsiSI CCGG 7 cut(s) 252, 363, 426, 684, 1037, 1145, 1367
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 5 cut(s) 432, 647, 1165, 1166, 1361
BsmAI GTCTC 4 cut(s) 391, 591, 631, 1265
BsmBI CGTCTC 1 cut(s) 591
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 1 cut(s) 663
Bso31I GGTCTC 2 cut(s) 391, 631
BsoBI CYCGRG 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 369
Bsp1407I TGTACA 1 cut(s) 270
Bsp143I GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
BspACI CCGC 1 cut(s) 1133
BspANI GGCC 1 cut(s) 663
BspCNI CTCAG 2 cut(s) 536, 621
BspDI ATCGAT 1 cut(s) 1112
BspPI GGATC 1 cut(s) 955
BspTNI GGTCTC 2 cut(s) 391, 631
BsrDI GCAATG 2 cut(s) 1392, 1431
BsrFI RCCGGY 2 cut(s) 425, 1144
BsrGI TGTACA 1 cut(s) 270
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 2 cut(s) 425, 1144
BssECI CCNNGG 2 cut(s) 1159, 1355
BssMI GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
BssNAI GTATAC 1 cut(s) 1142
Bst1107I GTATAC 1 cut(s) 1142
Bst2UI CCWGG 2 cut(s) 1161, 1356
Bst4CI ACNGT 4 cut(s) 418, 592, 992, 1255
Bst6I CTCTTC 3 cut(s) 517, 881, 890
BstAUI TGTACA 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 3 cut(s) 523, 629, 1287
BstF5I GGATG 6 cut(s) 486, 841, 949, 1311, 1413, 1443
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 6 cut(s) 96, 531, 670, 950, 1112, 1284
BstMAI GTCTC 4 cut(s) 391, 591, 631, 1265
BstMBI GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
BstMCI CGRYCG 1 cut(s) 664
BstNI CCWGG 2 cut(s) 1161, 1356
BstNSI RCATGY 2 cut(s) 191, 271
BstSCI CCNGG 3 cut(s) 362, 1159, 1354
BstV2I GAAGAC 1 cut(s) 1063
BstX2I RGATCY 1 cut(s) 947
BstXI CCANNNNNNTGG 1 cut(s) 24
BstYI RGATCY 1 cut(s) 947
BstZ17I GTATAC 1 cut(s) 1142
BstZI CGGCCG 1 cut(s) 661
Bsu15I ATCGAT 1 cut(s) 1112
BsuI GTATCC 2 cut(s) 235, 572
BsuRI GGCC 1 cut(s) 663
BsuTUI ATCGAT 1 cut(s) 1112
BtsCI GGATG 6 cut(s) 486, 841, 949, 1311, 1413, 1443
BtsI GCAGTG 2 cut(s) 980, 1404
BtsIMutI CAGTG 2 cut(s) 980, 1404
Cac8I GCNNGC 1 cut(s) 618
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 2 cut(s) 425, 1144
Cfr13I GGNCC 3 cut(s) 371, 926, 1364
ClaI ATCGAT 1 cut(s) 1112
Csp6I GTAC 5 cut(s) 271, 436, 658, 1099, 1213
CspAI ACCGGT 2 cut(s) 425, 1144
CspCI CAANNNNNGTGG 2 cut(s) 363, 398
CviAII CATG 4 cut(s) 188, 268, 352, 1514
CviQI GTAC 5 cut(s) 271, 436, 658, 1099, 1213
DdeI CTNAG 3 cut(s) 523, 629, 1287
DpnI GATC 6 cut(s) 95, 530, 669, 949, 1111, 1283
DpnII GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
EaeI YGGCCR 1 cut(s) 661
EagI CGGCCG 1 cut(s) 661
Eam1104I CTCTTC 3 cut(s) 517, 881, 890
EarI CTCTTC 3 cut(s) 517, 881, 890
EclXI CGGCCG 1 cut(s) 661
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 2 cut(s) 391, 631
Eco47I GGWCC 3 cut(s) 371, 926, 1364
Eco52I CGGCCG 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 1138
Eco88I CYCGRG 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 761
EcoRII CCWGG 2 cut(s) 1159, 1354
EcoT38I GRGCYC 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 591
FaeI CATG 4 cut(s) 191, 271, 355, 1517
FaqI GGGAC 1 cut(s) 521
FatI CATG 4 cut(s) 187, 267, 351, 1513
FauI CCCGC 1 cut(s) 1126
FblI GTMKAC 1 cut(s) 1141
FokI GGATG 6 cut(s) 493, 828, 956, 1318, 1420, 1450
FriOI GRGCYC 1 cut(s) 369
FspBI CTAG 1 cut(s) 518
GlaI GCGC 1 cut(s) 250
HaeIII GGCC 1 cut(s) 663
HapII CCGG 7 cut(s) 252, 363, 426, 684, 1037, 1145, 1367
HhaI GCGC 1 cut(s) 251
Hin1II CATG 4 cut(s) 191, 271, 355, 1517
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 8 cut(s) 207, 408, 725, 769, 820, 1094, 1121, 1126
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 7 cut(s) 252, 363, 426, 684, 1037, 1145, 1367
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 7 cut(s) 127, 320, 374, 1079, 1101, 1142, 1222
Hpy188I TCNGA 7 cut(s) 88, 212, 391, 473, 730, 774, 1107
Hpy188III TCNNGA 5 cut(s) 706, 716, 766, 1089, 1264
Hpy8I GTNNAC 7 cut(s) 127, 320, 374, 1079, 1101, 1142, 1222
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 4 cut(s) 154, 1045, 1095, 1442
HpyCH4III ACNGT 4 cut(s) 418, 592, 992, 1255
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 3 cut(s) 983, 999, 1397
HpyF3I CTNAG 3 cut(s) 523, 629, 1287
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 4 cut(s) 191, 271, 355, 1517
HspAI GCGC 1 cut(s) 249
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
LweI GCATC 4 cut(s) 738, 992, 1372, 1398
MaeI CTAG 1 cut(s) 518
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 4 cut(s) 566, 586, 679, 1169
MalI GATC 6 cut(s) 95, 530, 669, 949, 1111, 1283
MboI GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
MflI RGATCY 1 cut(s) 947
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 7 cut(s) 358, 449, 602, 761, 812, 938, 1063
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 1 cut(s) 901
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 8 cut(s) 126, 413, 854, 1008, 1020, 1041, 1431, 1528
MslI CAYNNNNRTG 3 cut(s) 192, 266, 1397
MspI CCGG 7 cut(s) 252, 363, 426, 684, 1037, 1145, 1367
MspR9I CCNGG 3 cut(s) 364, 1161, 1356
MvaI CCWGG 2 cut(s) 1161, 1356
NciI CCSGG 1 cut(s) 364
NdeII GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
NlaIII CATG 4 cut(s) 191, 271, 355, 1517
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 3 cut(s) 566, 586, 679
NspI RCATGY 2 cut(s) 191, 271
PaeR7I CTCGAG 1 cut(s) 405
PciI ACATGT 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 1094
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 7 cut(s) 207, 725, 769, 820, 1094, 1121, 1126
PflMI CCANNNNNTGG 1 cut(s) 1361
PinAI ACCGGT 2 cut(s) 425, 1144
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
PscI ACATGT 1 cut(s) 267
PsiI TTATAA 1 cut(s) 968
Psp6I CCWGG 2 cut(s) 1159, 1354
PspGI CCWGG 2 cut(s) 1159, 1354
PspPI GGNCC 3 cut(s) 371, 926, 1364
PspXI VCTCGAGB 1 cut(s) 405
PsuI RGATCY 1 cut(s) 947
RsaI GTAC 5 cut(s) 272, 437, 659, 1100, 1214
RsaNI GTAC 5 cut(s) 271, 436, 658, 1099, 1213
RseI CAYNNNNRTG 3 cut(s) 192, 266, 1397
SaqAI TTAA 8 cut(s) 126, 413, 854, 1008, 1020, 1041, 1431, 1528
Sau3AI GATC 6 cut(s) 93, 528, 667, 947, 1109, 1281
Sau96I GGNCC 3 cut(s) 371, 926, 1364
ScaI AGTACT 1 cut(s) 1214
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 3 cut(s) 364, 1161, 1356
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 4 cut(s) 738, 992, 1372, 1398
Sfr274I CTCGAG 1 cut(s) 405
SinI GGWCC 3 cut(s) 371, 926, 1364
SlaI CTCGAG 1 cut(s) 405
SmiMI CAYNNNNRTG 3 cut(s) 192, 266, 1397
SmlI CTYRAG 6 cut(s) 37, 224, 405, 1262, 1412, 1502
SmoI CTYRAG 6 cut(s) 37, 224, 405, 1262, 1412, 1502
Sse9I AATT 7 cut(s) 358, 449, 602, 761, 812, 938, 1063
SsiI CCGC 1 cut(s) 1133
SspI AATATT 1 cut(s) 1270
SspMI CTAG 1 cut(s) 518
StyD4I CCNGG 3 cut(s) 362, 1159, 1354
TaaI ACNGT 4 cut(s) 418, 592, 992, 1255
TaiI ACGT 1 cut(s) 588
TaqI TCGA 8 cut(s) 96, 336, 406, 767, 789, 1112, 1119, 1124
TaqII GACCGA 1 cut(s) 359
TasI AATT 7 cut(s) 358, 449, 602, 761, 812, 938, 1063
TatI WGTACW 2 cut(s) 270, 1212
TfiI GAWTC 7 cut(s) 207, 725, 769, 820, 1094, 1121, 1126
Tru1I TTAA 8 cut(s) 126, 413, 854, 1008, 1020, 1041, 1431, 1528
Tru9I TTAA 8 cut(s) 126, 413, 854, 1008, 1020, 1041, 1431, 1528
TscAI CASTG 2 cut(s) 980, 1404
TseFI GTSAC 3 cut(s) 566, 586, 679
Tsp45I GTSAC 3 cut(s) 566, 586, 679
TspDTI ATGAA 7 cut(s) 20, 340, 497, 735, 1064, 1251, 1530
TspGWI ACGGA 2 cut(s) 254, 703
TspRI CASTG 2 cut(s) 980, 1404
Van91I CCANNNNNTGG 1 cut(s) 1361
VpaK11BI GGWCC 3 cut(s) 371, 926, 1364
XapI RAATTY 3 cut(s) 761, 812, 1063
XceI RCATGY 2 cut(s) 191, 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
XhoI CTCGAG 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 1141
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 1 cut(s) 518
ZrmI AGTACT 1 cut(s) 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.