AT2G39980

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0017170
Physical Location & Seq
Reverse (-)
16687673 .. 16690182
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G39980.1

Sequence Viewer

Length: 1449 bp
ATGCCTTCTTTAGAGAAATCTGTAACAATAATCTCAAGAAACAGAGTCTTCCCAGACCAAAAGTCAACCCTCGTTGACCTCAAGCTCTCCGTCTCCGATCTTCCCATGCTCTCTTGCCACTACATCCAAAAAGGTTGTCTTTTCACGTGCCCTAACCTTCCTCTCCCCGCGCTTATCTCACACCTCAAACACTCTCTCTCAATCACTCTCACCCACTTCCCTCCTCTCGCTGGCCGTCTCTCAACCTCCTCCTCCGGCCACGTTTTCCTCACCTGCAATGACGCCGGCGCCGATTTTGTTTTCGCCCAAGCAAAATCCATCCACGTGAGCGACGTGATCGCCGGAATCGACGTTCCCGATGTAGTCAAAGAGTTTTTCACCTACGACAGAGCTGTGAGCTACGAGGGACATAACAGACCAATCCTCGCCGTTCAAGTGACGGAACTGAACGACGGCGTTTTCATCGGATGCTCCGTTAACCACGCCGTGACTGACGGAACCTCGCTGTGGAATTTCATTAACACCTTCGCGGAGGTTTCTAGAGGTGCTAAGAATGTGACACGTCAGCCTGATTTTACGCGGGAGTCTGTGCTAATCTCACCGGCTGTGCTCAAAGTCCCTCAAGGTGGTCCAAAGGTGACATTTGACGAAAACGCGCCGTTACGTGAACGGATATTCAGTTTCAGTAGAGAATCGATTCAAGAGCTTAAAGCTGTGGTGAACAAGAAGAAATGGTTGACGGTTGATAACGGCGAAATAGACGGTGTTGAGTTGTTAGGGAAACAAAGCAACGATAAACTTAACGGAAAAGAAAACGGTATTTTAACGGAAATGTTGGAGAGTTTGTTTGGGAGAAACGACGCCGTTTCGAAACCCGTCGCGGTTGAGATCTCGTCGTTTCAATCTCTATGCGCGTTGCTTTGGCGTGCGATCACACGTGCGAGGAAGCTTCCGAGTTCGAAAACGACTACGTTTCGTATGGCTGTGAATTGTCGTCACCGTTTGAGTCCTAAACTGAATCCGGAGTATTTCGGAAACGCGATTCAAAGCGTTCCTACGTTTGCGACTGCGGCAGAGGTTTTGTCACGTGATCTTAAATGGTGCGCTGATCAGCTTAACCAGAGTGTTGCGGCTCATCAAGACGGGAGGATTCGTAGCGTTGTGGCGGATTGGGAAGCGAATCCCAGGTGTTTTCCTCTTGGAAATGCCGACGGAGCTTCGGTTACTATGGGAAGCTCTCCGAGGTTTCCGATGTATGATAATGATTTCGGGTGGGGAAGGCCGGTGGCGGTTAGAAGTGGACGGTCGAATAAGTTTGATGGGAAGATTTCGGCGTTTCCGGGGAGGGAAGGAAACGGGACTGTTGATTTGGAGGTGGTTTTATCGCCGGAGACTATGGCTGGGATTGAATCTGACGGTGAGTTTATGCGATACGTGACTAATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

482

Amino Acids

52.88

Weight (kDa)

8.02

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 7 - 476 2e-149 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39980
fragaria_vesca FvH4_7g24060
malus_domestica MD01G1147800.v1.1 MD07G1214800.v1.1
prunus_persica Prupe.2G246800_v2.0.a1
pyrus_communis pycom07g19720
rosa_chinensis RchiOBHm_Chr1g0369491
rosa_laevigata RLG00000027124
rosa_multiflora Rmu_ssc0000484.1_g000024
rosa_roxburghii Rroxscaffold_4G00287590
rosa_rugosa Rorug01G0351000
rosa_samantha Rh1AG358900
rosa_wichuraiana Rw1G031610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 7 cut(s) 170, 530, 580, 656, 881, 914, 1040
AccIII TCCGGA 1 cut(s) 1021
AciI CCGC 8 cut(s) 168, 530, 580, 881, 1070, 1130, 1166, 1289
AcoI YGGCCR 2 cut(s) 232, 256
AcsI RAATTY 1 cut(s) 511
AcvI CACGTG 4 cut(s) 147, 325, 938, 1088
AcyI GRCGYC 3 cut(s) 282, 288, 861
AdeI CACNNNGTG 1 cut(s) 487
AfiI CCNNNNNNNGG 3 cut(s) 230, 507, 626
AflIII ACRYGT 2 cut(s) 560, 935
AgsI TTSAA 5 cut(s) 434, 701, 902, 1046, 1409
AhdI GACNNNNNGTC 1 cut(s) 61
AjiI CACGTC 2 cut(s) 334, 563
AjnI CCWGG 1 cut(s) 1184
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 9 cut(s) 85, 392, 399, 706, 713, 949, 1114, 1217, 1236
AluI AGCT 9 cut(s) 85, 392, 399, 706, 713, 949, 1114, 1217, 1236
Alw21I GWGCWC 1 cut(s) 612
Alw26I GTCTC 3 cut(s) 97, 242, 1385
Aor13HI TCCGGA 1 cut(s) 1021
AoxI GGCC 3 cut(s) 232, 256, 1280
ApoI RAATTY 1 cut(s) 511
ArsI GACNNNNNNTTYG 4 cut(s) 121, 153, 1351, 1383
Asp700I GAANNNNTTC 1 cut(s) 696
AspLEI GCGC 5 cut(s) 172, 290, 658, 914, 1106
AspS9I GGNCC 1 cut(s) 629
AsuC2I CCSGG 1 cut(s) 1341
AsuHPI GGTGA 8 cut(s) 202, 262, 370, 591, 649, 730, 989, 1430
AsuII TTCGAA 2 cut(s) 869, 959
AvaII GGWCC 1 cut(s) 629
BaeGI GKGCMC 1 cut(s) 152
BanI GGYRCC 1 cut(s) 287
BbrPI CACGTG 4 cut(s) 147, 325, 938, 1088
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 612
BccI CCATC 2 cut(s) 326, 1313
BceAI ACGGC 7 cut(s) 219, 413, 469, 470, 643, 766, 848
BciT130I CCWGG 1 cut(s) 1186
BclI TGATCA 1 cut(s) 1108
BcnI CCSGG 1 cut(s) 1341
BcoDI GTCTC 3 cut(s) 97, 242, 1385
BfaI CTAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 281
BglII AGATCT 1 cut(s) 888
BisI GCNGC 2 cut(s) 1071, 1131
BlsI GCNGC 2 cut(s) 1072, 1132
Bme1390I CCNGG 2 cut(s) 1186, 1341
Bme18I GGWCC 1 cut(s) 629
BmeRI GACNNNNNGTC 1 cut(s) 61
BmgBI CACGTC 2 cut(s) 334, 563
BmgT120I GGNCC 1 cut(s) 629
BmiI GGNNCC 2 cut(s) 289, 499
BmrFI CCNGG 2 cut(s) 1186, 1341
BmsI GCATC 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 40
Bpu14I TTCGAA 2 cut(s) 869, 959
BpuEI CTTGAG 3 cut(s) 19, 65, 606
BpuMI CCSGG 1 cut(s) 1341
Bsa29I ATCGAT 1 cut(s) 695
BsaAI YACGTR 6 cut(s) 147, 325, 665, 938, 1088, 1435
BsaHI GRCGYC 3 cut(s) 282, 288, 861
BsaJI CCNNGG 3 cut(s) 1184, 1241, 1340
BsaWI WCCGGW 1 cut(s) 1021
BsaXI ACNNNNNCTCC 6 cut(s) 830, 860, 1138, 1168, 1206, 1236
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 507, 626
Bse118I RCCGGY 3 cut(s) 284, 601, 1282
Bse3DI GCAATG 1 cut(s) 283
BseAI TCCGGA 1 cut(s) 1021
BseBI CCWGG 1 cut(s) 1186
BseCI ATCGAT 1 cut(s) 695
BseDI CCNNGG 3 cut(s) 1184, 1241, 1340
BseGI GGATG 3 cut(s) 123, 318, 473
BseLI CCNNNNNNNGG 3 cut(s) 230, 507, 626
BseMI GCAATG 1 cut(s) 283
BseRI GAGGAG 3 cut(s) 213, 238, 241
BseSI GKGCMC 1 cut(s) 152
BseYI CCCAGC 1 cut(s) 1400
Bsh1236I CGCG 7 cut(s) 170, 530, 580, 656, 881, 914, 1040
Bsh1285I CGRYCG 1 cut(s) 1307
BshFI GGCC 3 cut(s) 234, 258, 1282
BshNI GGYRCC 1 cut(s) 287
BshVI ATCGAT 1 cut(s) 695
BsiEI CGRYCG 1 cut(s) 1307
BsiHKAI GWGCWC 1 cut(s) 612
BsiSI CCGG 8 cut(s) 255, 285, 342, 602, 1022, 1283, 1340, 1388
BslFI GGGAC 3 cut(s) 420, 602, 1372
BslI CCNNNNNNNGG 3 cut(s) 230, 507, 626
BsmAI GTCTC 3 cut(s) 97, 242, 1385
BsmBI CGTCTC 2 cut(s) 97, 242
BsmFI GGGAC 3 cut(s) 420, 602, 1372
BsnI GGCC 3 cut(s) 234, 258, 1282
Bsp119I TTCGAA 2 cut(s) 869, 959
Bsp1286I GDGCHC 2 cut(s) 152, 612
Bsp13I TCCGGA 1 cut(s) 1021
Bsp143I GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
BspACI CCGC 8 cut(s) 168, 530, 580, 881, 1070, 1130, 1166, 1289
BspANI GGCC 3 cut(s) 234, 258, 1282
BspDI ATCGAT 1 cut(s) 695
BspEI TCCGGA 1 cut(s) 1021
BspFNI CGCG 7 cut(s) 170, 530, 580, 656, 881, 914, 1040
BspLI GGNNCC 2 cut(s) 289, 499
BspMI ACCTGC 1 cut(s) 281
BspT104I TTCGAA 2 cut(s) 869, 959
BspT107I GGYRCC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 283
BsrFI RCCGGY 3 cut(s) 284, 601, 1282
BssAI RCCGGY 3 cut(s) 284, 601, 1282
BssECI CCNNGG 3 cut(s) 1184, 1241, 1340
BssMI GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
BssNI GRCGYC 3 cut(s) 282, 288, 861
Bst2UI CCWGG 1 cut(s) 1186
Bst4CI ACNGT 7 cut(s) 742, 764, 818, 1001, 1305, 1363, 1418
BstACI GRCGYC 3 cut(s) 282, 288, 861
BstBAI YACGTR 6 cut(s) 147, 325, 665, 938, 1088, 1435
BstBI TTCGAA 2 cut(s) 869, 959
BstC8I GCNNGC 3 cut(s) 232, 286, 927
BstDEI CTNAG 1 cut(s) 549
BstF5I GGATG 3 cut(s) 123, 318, 473
BstFNI CGCG 7 cut(s) 170, 530, 580, 656, 881, 914, 1040
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 5 cut(s) 172, 290, 658, 914, 1106
BstKTI GATC 6 cut(s) 100, 339, 891, 933, 1093, 1111
BstMAI GTCTC 3 cut(s) 97, 242, 1385
BstMBI GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
BstMCI CGRYCG 1 cut(s) 1307
BstMWI GCNNNNNNNGC 2 cut(s) 1070, 1214
BstNI CCWGG 1 cut(s) 1186
BstSCI CCNGG 2 cut(s) 1184, 1339
BstSLI GKGCMC 1 cut(s) 152
BstUI CGCG 7 cut(s) 170, 530, 580, 656, 881, 914, 1040
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 888
BstYI RGATCY 1 cut(s) 888
Bsu15I ATCGAT 1 cut(s) 695
BsuRI GGCC 3 cut(s) 234, 258, 1282
BsuTUI ATCGAT 1 cut(s) 695
BtrI CACGTC 2 cut(s) 334, 563
BtsCI GGATG 3 cut(s) 123, 318, 473
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 3 cut(s) 232, 286, 927
CfoI GCGC 5 cut(s) 172, 290, 658, 914, 1106
Cfr10I RCCGGY 3 cut(s) 284, 601, 1282
Cfr13I GGNCC 1 cut(s) 629
ClaI ATCGAT 1 cut(s) 695
CseI GACGC 2 cut(s) 290, 869
CviAII CATG 1 cut(s) 106
DdeI CTNAG 1 cut(s) 549
DinI GGCGCC 1 cut(s) 289
DpnI GATC 6 cut(s) 99, 338, 890, 932, 1092, 1110
DpnII GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
DraIII CACNNNGTG 1 cut(s) 487
DriI GACNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 2 cut(s) 232, 256
Eam1105I GACNNNNNGTC 1 cut(s) 61
EciI GGCGGA 1 cut(s) 1181
Eco47I GGWCC 1 cut(s) 629
Eco72I CACGTG 4 cut(s) 147, 325, 938, 1088
EcoRII CCWGG 1 cut(s) 1184
EgeI GGCGCC 1 cut(s) 289
EheI GGCGCC 1 cut(s) 289
Esp3I CGTCTC 2 cut(s) 97, 242
FaeI CATG 1 cut(s) 109
FaiI YATR 8 cut(s) 107, 411, 910, 980, 1229, 1257, 1397, 1427
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 3 cut(s) 420, 602, 1372
FatI CATG 1 cut(s) 105
FauI CCCGC 2 cut(s) 175, 573
FbaI TGATCA 1 cut(s) 1108
Fnu4HI GCNGC 2 cut(s) 1071, 1131
FokI GGATG 3 cut(s) 110, 305, 480
Fsp4HI GCNGC 2 cut(s) 1071, 1131
FspBI CTAG 1 cut(s) 540
GlaI GCGC 5 cut(s) 171, 289, 657, 913, 1105
GluI GCNGC 2 cut(s) 1071, 1131
GsaI CCCAGC 1 cut(s) 1404
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 3 cut(s) 234, 258, 1282
HapII CCGG 8 cut(s) 255, 285, 342, 602, 1022, 1283, 1340, 1388
HgaI GACGC 2 cut(s) 290, 869
HhaI GCGC 5 cut(s) 172, 290, 658, 914, 1106
Hin1I GRCGYC 3 cut(s) 282, 288, 861
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 5 cut(s) 170, 288, 656, 912, 1104
HinP1I GCGC 5 cut(s) 170, 288, 656, 912, 1104
HincII GTYRAC 4 cut(s) 66, 76, 478, 738
HindII GTYRAC 4 cut(s) 66, 76, 478, 738
HindIII AAGCTT 1 cut(s) 947
HpaI GTTAAC 1 cut(s) 478
HpaII CCGG 8 cut(s) 255, 285, 342, 602, 1022, 1283, 1340, 1388
HphI GGTGA 8 cut(s) 202, 262, 370, 591, 649, 730, 989, 1430
Hpy166II GTNNAC 7 cut(s) 66, 76, 478, 668, 721, 738, 1301
Hpy188I TCNGA 7 cut(s) 97, 467, 954, 1034, 1242, 1251, 1414
Hpy188III TCNNGA 6 cut(s) 36, 356, 540, 701, 1022, 1139
Hpy8I GTNNAC 7 cut(s) 66, 76, 478, 668, 721, 738, 1301
Hpy99I CGWCG 7 cut(s) 335, 353, 455, 863, 881, 898, 1214
HpyAV CCTTC 5 cut(s) 15, 167, 535, 1272, 1343
HpyCH4III ACNGT 7 cut(s) 742, 764, 818, 1001, 1305, 1363, 1418
HpyCH4V TGCA 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 1070, 1214
HpyF3I CTNAG 1 cut(s) 549
Hsp92I GRCGYC 3 cut(s) 282, 288, 861
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 5 cut(s) 170, 288, 656, 912, 1104
KasI GGCGCC 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 1021
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 1108
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
LmnI GCTCC 2 cut(s) 476, 1214
LweI GCATC 1 cut(s) 458
MaeI CTAG 1 cut(s) 540
MalI GATC 6 cut(s) 99, 338, 890, 932, 1092, 1110
MboI GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
MboII GAAGA 4 cut(s) 40, 92, 739, 1336
MflI RGATCY 1 cut(s) 888
MhlI GDGCHC 2 cut(s) 152, 612
MluCI AATT 2 cut(s) 511, 988
Mly113I GGCGCC 1 cut(s) 288
MlyI GAGTC 3 cut(s) 54, 593, 1015
MmeI TCCRAC 1 cut(s) 816
MreI CGCCGGCG 1 cut(s) 284
MroI TCCGGA 1 cut(s) 1021
MroNI GCCGGC 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 696
MseI TTAA 7 cut(s) 477, 519, 708, 801, 824, 1095, 1116
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 8 cut(s) 255, 285, 342, 602, 1022, 1283, 1340, 1388
MspR9I CCNGG 2 cut(s) 1186, 1341
MvaI CCWGG 1 cut(s) 1186
MvnI CGCG 7 cut(s) 170, 530, 580, 656, 881, 914, 1040
MwoI GCNNNNNNNGC 2 cut(s) 1070, 1214
NaeI GCCGGC 1 cut(s) 286
NarI GGCGCC 1 cut(s) 288
NciI CCSGG 1 cut(s) 1341
NdeII GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
NgoMIV GCCGGC 1 cut(s) 284
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 2 cut(s) 289, 499
NmuCI GTSAC 7 cut(s) 436, 487, 556, 637, 995, 1083, 1435
NspV TTCGAA 2 cut(s) 869, 959
PaqCI CACCTGC 1 cut(s) 281
PcsI WCGNNNNNNNCGW 5 cut(s) 288, 330, 345, 354, 471
PdiI GCCGGC 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 696
PfeI GAWTC 8 cut(s) 345, 692, 697, 1018, 1042, 1150, 1180, 1409
PkrI GCNGC 2 cut(s) 1072, 1132
PleI GAGTC 3 cut(s) 53, 592, 1014
PluTI GGCGCC 1 cut(s) 291
PmaCI CACGTG 4 cut(s) 147, 325, 938, 1088
PmlI CACGTG 4 cut(s) 147, 325, 938, 1088
PpsI GAGTC 3 cut(s) 53, 592, 1014
Ppu21I YACGTR 6 cut(s) 147, 325, 665, 938, 1088, 1435
Psp6I CCWGG 1 cut(s) 1184
PspCI CACGTG 4 cut(s) 147, 325, 938, 1088
PspFI CCCAGC 1 cut(s) 1400
PspGI CCWGG 1 cut(s) 1184
PspN4I GGNNCC 2 cut(s) 289, 499
PspPI GGNCC 1 cut(s) 629
PsuI RGATCY 1 cut(s) 888
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 7 cut(s) 477, 519, 708, 801, 824, 1095, 1116
SatI GCNGC 2 cut(s) 1071, 1131
Sau3AI GATC 6 cut(s) 97, 336, 888, 930, 1090, 1108
Sau96I GGNCC 1 cut(s) 629
SchI GAGTC 3 cut(s) 54, 593, 1015
ScrFI CCNGG 2 cut(s) 1186, 1341
SduI GDGCHC 2 cut(s) 152, 612
SfaNI GCATC 1 cut(s) 458
SfoI GGCGCC 1 cut(s) 289
SfuI TTCGAA 2 cut(s) 869, 959
SgrAI CRCCGGYG 1 cut(s) 284
SinI GGWCC 1 cut(s) 629
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 3 cut(s) 34, 80, 621
SmoI CTYRAG 3 cut(s) 34, 80, 621
Sse9I AATT 2 cut(s) 511, 988
SsiI CCGC 8 cut(s) 168, 530, 580, 881, 1070, 1130, 1166, 1289
SspDI GGCGCC 1 cut(s) 287
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 2 cut(s) 1184, 1339
TaaI ACNGT 7 cut(s) 742, 764, 818, 1001, 1305, 1363, 1418
TaqI TCGA 5 cut(s) 348, 695, 869, 959, 1307
TasI AATT 2 cut(s) 511, 988
TauI GCSGC 2 cut(s) 1073, 1133
TfiI GAWTC 8 cut(s) 345, 692, 697, 1018, 1042, 1150, 1180, 1409
Tru1I TTAA 7 cut(s) 477, 519, 708, 801, 824, 1095, 1116
Tru9I TTAA 7 cut(s) 477, 519, 708, 801, 824, 1095, 1116
TseFI GTSAC 7 cut(s) 436, 487, 556, 637, 995, 1083, 1435
Tsp45I GTSAC 7 cut(s) 436, 487, 556, 637, 995, 1083, 1435
TspDTI ATGAA 2 cut(s) 451, 505
TspGWI ACGGA 8 cut(s) 79, 455, 463, 510, 685, 819, 842, 1227
VpaK11BI GGWCC 1 cut(s) 629
XapI RAATTY 1 cut(s) 511
XbaI TCTAGA 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 696
XspI CTAG 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.