Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0017170

Physical Location & Seq

Genomic Coordinates Reverse (-)

8585766 .. 8587229

Gene Structure 1464 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00287590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGCCTTCTTCTTCGCCAATCTCTCTCACTGTGGTGTCTGAATCCATGGTGTTTCCTGACCAGACCTCCACCCTCGGAGACCTCAAGCTTTCGGTTTCTGACCTCCCCATGCTCTCATGTCACTACATCCAAAAGGGAGGCCTCTTCACTAAGCCTTCTTTCCCAATCCAATCCCTAGTCTCGCTACTCAAGACCGGCCTCTCCCAGACCCTCACTCGCTTCCCGCCCTTGGCCGGCCGGTTGACTACAGACAAAGATGGTTACTTGTACATCACCTGCAACGACTCCGGGGTTGATTTTATCCACGCAGCGGCTACTGAGGTCTCCGTGCGTGACATTCTGTCGCCGACTCACGTGCCGGAGTCGGTGAAGGAGTTTTTTGCATTGGACAGGACTGTGAGCTACACCGGCCATCACAACCCAATCCTGGCAGTCCAGGTCACCGAGCTCAAAGACGGCGTTTTCATCGGTTGCGCCGTCAACCACGCCGTCACTGACGGCACCTCCTTCTGGAACTTCTTCAACACCTTCGCCGAGGTCTGCCGCGGCGGCAAGAGCTTCTCCAGAAAGCCGGACTTCACCCGGGACTCGATTCTGATTTCTCAGGCCGTGCTTCAGTTGCCGGAAGGTGGTCCCAAGGTCACATTCAACGTCAACGAGCCGTTGACCGAGAGAATCTTCAGCTTCAGCAGAGAAGCCATTCTGAAACTAAAAGCCACAACCAACAACAACAACAACAAGAAATGGACCCAAAATGACGACGTCGCCACCGCCGTCGAGATTCTCGGGAAGCAGAGCAACGACCCTTACGAAACTAACAACAACAACGCCAGAGTAACCGCCATCATCGAAAACTGGTTCAAGAATTCCAAGCCGCAAATCACTGAGAACAAAACGGCAGCGGAGATCTCGTCGTTTCAGTCTCTCTGCGCGCTTCTGTGGAAGGCTGTGACGCGCGCCAGGAAGCTACCGGAGTCGAAAACGACGACGTTCAGAATGGCAGTGAACTGCCGTCACAGGCTGGAGCCGAAGCTAGACCCGTACTACTTCGGGAACGCAATCCAAAGTATCCCGACGTACGCGTCGTCGGGTGACGTGTTGTCTAGGGATCTGAGATGGAGCGCGGAGCAGCTCAACGACAACGTGAAGGCGCACGGTAGTGATAAGGTGAGGGAGTGCGTAGAGGATTGGGAGAAGAACCCGCGGGTGTTTCCGCTAGGGAACTTCGACGGTGCATCAATGACGATGGGCAGCTCGCCGAGGTTTCCGATGTACGACAACGATTTCGGGTGGGGCAGGCCGTTGGCTGTTCGGAGCGGGCGGGCGAATAAATTCGACGGTAAGATCTCGGCGTTTCCGGGGAGGGACGGAGCTGGGACCGTTGATCTGGAGGTGGTTTTGGCGCCTGACACGATGGCGGGGTTGGAGTCTGATTTGGAGTTCATGCAATACGTGTCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010143 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0043170 GO:0044424 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0051179 GO:0071704 GO:0071840 GO:0090558 GO:0090626 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 Transferase

Protein Analysis

487

Amino Acids

53.86

Weight (kDa)

6.28

Isoelectric Point (pI)

39.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transferase	PF02458	8 - 482	1.3e-113	Transferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017170)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39980
fragaria_vesca	FvH4_7g24060
malus_domestica	MD01G1147800.v1.1 MD07G1214800.v1.1
prunus_persica	Prupe.2G246800_v2.0.a1
pyrus_communis	pycom07g19720
rosa_chinensis	RchiOBHm_Chr1g0369491
rosa_laevigata	RLG00000027124
rosa_multiflora	Rmu_ssc0000484.1_g000024
rosa_roxburghii	Rroxscaffold_4G00287590
rosa_rugosa	Rorug01G0351000
rosa_samantha	Rh1AG358900
rosa_wichuraiana	Rw1G031610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	284
AasI	GACNNNNNNGTC	1 cut(s)	1081
AatII	GACGTC	1 cut(s)	765
Acc36I	ACCTGC	1 cut(s)	284
AccB1I	GGYRCC	2 cut(s)	500, 1402
AccBSI	CCGCTC	1 cut(s)	1317
AccII	CGCG	7 cut(s)	546, 932, 955, 957, 1082, 1124, 1204
AclWI	GGATC	1 cut(s)	1116
AcoI	YGGCCR	3 cut(s)	231, 235, 409
AcsI	RAATTY	2 cut(s)	865, 1331
AcuI	CTGAAG	3 cut(s)	599, 664, 670
AcvI	CACGTG	1 cut(s)	355
AcyI	GRCGYC	2 cut(s)	762, 1403
AfaI	GTAC	4 cut(s)	269, 1043, 1079, 1274
AfiI	CCNNNNNNNGG	6 cut(s)	229, 233, 310, 427, 510, 629
AflIII	ACRYGT	3 cut(s)	1080, 1095, 1452
AgsI	TTSAA	3 cut(s)	523, 649, 862
AhdI	GACNNNNNGTC	2 cut(s)	340, 1099
AjiI	CACGTC	1 cut(s)	1096
AjnI	CCWGG	3 cut(s)	426, 435, 959
AjuI	GAANNNNNNNTTGG	2 cut(s)	629, 661
AleI	CACNNNNGTG	2 cut(s)	32, 1158
Alw21I	GWGCWC	1 cut(s)	450
Alw26I	GTCTC	4 cut(s)	72, 184, 328, 927
AlwI	GGATC	1 cut(s)	1116
Ama87I	CYCGRG	2 cut(s)	582, 785
AoxI	GGCC	7 cut(s)	139, 196, 231, 235, 409, 606, 1298
ApeKI	GCWGC	4 cut(s)	308, 899, 1129, 1251
ApoI	RAATTY	2 cut(s)	865, 1331
ArsI	GACNNNNNNTTYG	2 cut(s)	1268, 1300
Asp700I	GAANNNNTTC	3 cut(s)	518, 699, 1331
AspLEI	GCGC	8 cut(s)	476, 932, 934, 957, 959, 1124, 1153, 1405
AspS9I	GGNCC	3 cut(s)	632, 747, 1377
AsuC2I	CCSGG	4 cut(s)	289, 583, 584, 1359
AsuHPI	GGTGA	6 cut(s)	265, 379, 433, 571, 1103, 1180
AvaI	CYCGRG	2 cut(s)	582, 785
AvaII	GGWCC	3 cut(s)	632, 747, 1377
BanI	GGYRCC	2 cut(s)	500, 1402
BanII	GRGCYC	1 cut(s)	450
BbrPI	CACGTG	1 cut(s)	355
Bbv12I	GWGCWC	1 cut(s)	450
BbvI	GCAGC	4 cut(s)	320, 911, 1141, 1263
BccI	CCATC	6 cut(s)	251, 420, 851, 1110, 1240, 1408
BcgI	CGANNNNNNTGC	2 cut(s)	337, 371
BciT130I	CCWGG	3 cut(s)	428, 437, 961
BciVI	GTATCC	1 cut(s)	1079
BcnI	CCSGG	4 cut(s)	289, 583, 584, 1359
BcoDI	GTCTC	4 cut(s)	72, 184, 328, 927
BfaI	CTAG	4 cut(s)	176, 1034, 1104, 1217
BfmI	CTRYAG	1 cut(s)	246
BfoI	RGCGCY	1 cut(s)	1406
BfuAI	ACCTGC	1 cut(s)	284
BfuI	GTATCC	1 cut(s)	1079
BglI	GCCNNNNNGGC	1 cut(s)	549
BglII	AGATCT	2 cut(s)	906, 1344
BisI	GCNGC	9 cut(s)	309, 312, 544, 547, 550, 875, 900, 1130, 1252
BlsI	GCNGC	9 cut(s)	310, 313, 545, 548, 551, 876, 901, 1131, 1253
Bme1390I	CCNGG	7 cut(s)	289, 428, 437, 583, 584, 961, 1359
Bme18I	GGWCC	3 cut(s)	632, 747, 1377
BmeRI	GACNNNNNGTC	2 cut(s)	340, 1099
BmeT110I	CYCGRG	2 cut(s)	582, 785
BmgBI	CACGTC	1 cut(s)	1096
BmgT120I	GGNCC	3 cut(s)	632, 747, 1377
BmiI	GGNNCC	6 cut(s)	502, 634, 749, 1026, 1378, 1404
BmrFI	CCNGG	7 cut(s)	289, 428, 437, 583, 584, 961, 1359
BmsI	GCATC	1 cut(s)	1244
BpmI	CTGGAG	3 cut(s)	547, 1043, 1409
BpuEI	CTTGAG	2 cut(s)	68, 173
BpuMI	CCSGG	4 cut(s)	289, 583, 584, 1359
BsaAI	YACGTR	2 cut(s)	355, 1453
BsaHI	GRCGYC	2 cut(s)	762, 1403
BsaI	GGTCTC	2 cut(s)	72, 328
BsaWI	WCCGGW	1 cut(s)	970
BsaXI	ACNNNNNCTCC	6 cut(s)	50, 80, 488, 518, 896, 926
Bsc4I	CCNNNNNNNGG	6 cut(s)	229, 233, 310, 427, 510, 629
Bse118I	RCCGGY	4 cut(s)	194, 233, 237, 407
Bse1I	ACTGG	1 cut(s)	860
BseBI	CCWGG	3 cut(s)	428, 437, 961
BseGI	GGATG	1 cut(s)	126
BseLI	CCNNNNNNNGG	6 cut(s)	229, 233, 310, 427, 510, 629
BseMII	CTCAG	4 cut(s)	309, 617, 876, 1103
BseNI	ACTGG	1 cut(s)	860
BsePI	GCGCGC	2 cut(s)	930, 955
BseX3I	CGGCCG	1 cut(s)	235
BseXI	GCAGC	4 cut(s)	320, 911, 1141, 1263
BseYI	CCCAGC	1 cut(s)	1373
Bsh1236I	CGCG	7 cut(s)	546, 932, 955, 957, 1082, 1124, 1204
Bsh1285I	CGRYCG	1 cut(s)	238
BshFI	GGCC	7 cut(s)	141, 198, 233, 237, 411, 608, 1300
BshNI	GGYRCC	2 cut(s)	500, 1402
BsiEI	CGRYCG	1 cut(s)	238
BsiHKAI	GWGCWC	1 cut(s)	450
BsiHKCI	CYCGRG	2 cut(s)	582, 785
BsiWI	CGTACG	1 cut(s)	1077
BslFI	GGGAC	4 cut(s)	599, 618, 1379, 1390
BslI	CCNNNNNNNGG	6 cut(s)	229, 233, 310, 427, 510, 629
BsmAI	GTCTC	4 cut(s)	72, 184, 328, 927
BsmFI	GGGAC	4 cut(s)	599, 618, 1379, 1390
BsnI	GGCC	7 cut(s)	141, 198, 233, 237, 411, 608, 1300
Bso31I	GGTCTC	2 cut(s)	72, 328
BsoBI	CYCGRG	2 cut(s)	582, 785
Bsp1286I	GDGCHC	1 cut(s)	450
Bsp1407I	TGTACA	1 cut(s)	267
Bsp143I	GATC	4 cut(s)	906, 1108, 1344, 1384
Bsp19I	CCATGG	1 cut(s)	45
BspANI	GGCC	7 cut(s)	141, 198, 233, 237, 411, 608, 1300
BspCNI	CTCAG	4 cut(s)	310, 616, 877, 1104
BspFNI	CGCG	7 cut(s)	546, 932, 955, 957, 1082, 1124, 1204
BspLI	GGNNCC	6 cut(s)	502, 634, 749, 1026, 1378, 1404
BspMI	ACCTGC	1 cut(s)	284
BspPI	GGATC	1 cut(s)	1116
BspT107I	GGYRCC	2 cut(s)	500, 1402
BspTNI	GGTCTC	2 cut(s)	72, 328
BsrBI	CCGCTC	1 cut(s)	1317
BsrFI	RCCGGY	4 cut(s)	194, 233, 237, 407
BsrGI	TGTACA	1 cut(s)	267
BsrI	ACTGG	1 cut(s)	860
BssAI	RCCGGY	4 cut(s)	194, 233, 237, 407
BssHII	GCGCGC	2 cut(s)	930, 955
BssMI	GATC	4 cut(s)	906, 1108, 1344, 1384
BssNI	GRCGYC	2 cut(s)	762, 1403
BssT1I	CCWWGG	3 cut(s)	45, 228, 636
Bst2UI	CCWGG	3 cut(s)	428, 437, 961
Bst4CI	ACNGT	6 cut(s)	31, 397, 1157, 1232, 1340, 1381
Bst6I	CTCTTC	1 cut(s)	149
BstACI	GRCGYC	2 cut(s)	762, 1403
BstAUI	TGTACA	1 cut(s)	267
BstBAI	YACGTR	2 cut(s)	355, 1453
BstC8I	GCNNGC	7 cut(s)	235, 932, 957, 1256, 1298, 1319, 1323
BstDEI	CTNAG	5 cut(s)	150, 318, 603, 885, 1112
BstDSI	CCRYGG	3 cut(s)	45, 544, 1202
BstEII	GGTNACC	1 cut(s)	439
BstF5I	GGATG	1 cut(s)	126
BstFNI	CGCG	7 cut(s)	546, 932, 955, 957, 1082, 1124, 1204
BstH2I	RGCGCY	1 cut(s)	1406
BstHHI	GCGC	8 cut(s)	476, 932, 934, 957, 959, 1124, 1153, 1405
BstKTI	GATC	4 cut(s)	909, 1111, 1347, 1387
BstMAI	GTCTC	4 cut(s)	72, 184, 328, 927
BstMBI	GATC	4 cut(s)	906, 1108, 1344, 1384
BstMCI	CGRYCG	1 cut(s)	238
BstMWI	GCNNNNNNNGC	4 cut(s)	408, 549, 555, 619
BstNI	CCWGG	3 cut(s)	428, 437, 961
BstPI	GGTNACC	1 cut(s)	439
BstSCI	CCNGG	7 cut(s)	287, 426, 435, 581, 582, 959, 1357
BstSFI	CTRYAG	1 cut(s)	246
BstUI	CGCG	7 cut(s)	546, 932, 955, 957, 1082, 1124, 1204
BstV1I	GCAGC	4 cut(s)	320, 911, 1141, 1263
BstX2I	RGATCY	3 cut(s)	906, 1108, 1344
BstYI	RGATCY	3 cut(s)	906, 1108, 1344
BstZI	CGGCCG	1 cut(s)	235
BsuI	GTATCC	1 cut(s)	1079
BsuRI	GGCC	7 cut(s)	141, 198, 233, 237, 411, 608, 1300
BtgI	CCRYGG	3 cut(s)	45, 544, 1202
BtrI	CACGTC	1 cut(s)	1096
BtsCI	GGATG	1 cut(s)	126
BtsI	GCAGTG	1 cut(s)	1008
BtsIMutI	CAGTG	4 cut(s)	27, 492, 882, 1008
BveI	ACCTGC	1 cut(s)	284
Cac8I	GCNNGC	7 cut(s)	235, 932, 957, 1256, 1298, 1319, 1323
CfoI	GCGC	8 cut(s)	476, 932, 934, 957, 959, 1124, 1153, 1405
Cfr10I	RCCGGY	4 cut(s)	194, 233, 237, 407
Cfr13I	GGNCC	3 cut(s)	632, 747, 1377
Cfr42I	CCGCGG	2 cut(s)	547, 1205
Cfr9I	CCCGGG	1 cut(s)	582
CseI	GACGC	2 cut(s)	961, 1071
Csp6I	GTAC	4 cut(s)	268, 1042, 1078, 1273
CviAII	CATG	4 cut(s)	46, 109, 117, 1444
CviQI	GTAC	4 cut(s)	268, 1042, 1078, 1273
DdeI	CTNAG	5 cut(s)	150, 318, 603, 885, 1112
DinI	GGCGCC	1 cut(s)	1404
DpnI	GATC	4 cut(s)	908, 1110, 1346, 1386
DpnII	GATC	4 cut(s)	906, 1108, 1344, 1384
DrdI	GACNNNNNNGTC	1 cut(s)	1081
DriI	GACNNNNNGTC	2 cut(s)	340, 1099
DseDI	GACNNNNNNGTC	1 cut(s)	1081
EaeI	YGGCCR	3 cut(s)	231, 235, 409
EagI	CGGCCG	1 cut(s)	235
Eam1104I	CTCTTC	1 cut(s)	149
Eam1105I	GACNNNNNGTC	2 cut(s)	340, 1099
EarI	CTCTTC	1 cut(s)	149
Ecl136II	GAGCTC	1 cut(s)	448
EclXI	CGGCCG	1 cut(s)	235
Eco130I	CCWWGG	3 cut(s)	45, 228, 636
Eco147I	AGGCCT	1 cut(s)	141
Eco24I	GRGCYC	1 cut(s)	450
Eco31I	GGTCTC	2 cut(s)	72, 328
Eco47I	GGWCC	3 cut(s)	632, 747, 1377
Eco52I	CGGCCG	1 cut(s)	235
Eco53kI	GAGCTC	1 cut(s)	448
Eco57I	CTGAAG	3 cut(s)	599, 664, 670
Eco72I	CACGTG	1 cut(s)	355
Eco88I	CYCGRG	2 cut(s)	582, 785
Eco91I	GGTNACC	1 cut(s)	439
EcoICRI	GAGCTC	1 cut(s)	448
EcoO65I	GGTNACC	1 cut(s)	439
EcoRI	GAATTC	1 cut(s)	865
EcoRII	CCWGG	3 cut(s)	426, 435, 959
EcoT14I	CCWWGG	3 cut(s)	45, 228, 636
EcoT38I	GRGCYC	1 cut(s)	450
EgeI	GGCGCC	1 cut(s)	1404
EheI	GGCGCC	1 cut(s)	1404
ErhI	CCWWGG	3 cut(s)	45, 228, 636
FaeI	CATG	4 cut(s)	49, 112, 120, 1447
FaiI	YATR	4 cut(s)	47, 110, 118, 1445
FalI	AAGNNNNNCTT	2 cut(s)	560, 592
FaqI	GGGAC	4 cut(s)	599, 618, 1379, 1390
FatI	CATG	4 cut(s)	45, 108, 116, 1443
FauI	CCCGC	6 cut(s)	231, 1197, 1209, 1310, 1314, 1411
Fnu4HI	GCNGC	9 cut(s)	309, 312, 544, 547, 550, 875, 900, 1130, 1252
FokI	GGATG	1 cut(s)	113
FriOI	GRGCYC	1 cut(s)	450
FseI	GGCCGGCC	1 cut(s)	237
Fsp4HI	GCNGC	9 cut(s)	309, 312, 544, 547, 550, 875, 900, 1130, 1252
FspBI	CTAG	4 cut(s)	176, 1034, 1104, 1217
GlaI	GCGC	8 cut(s)	475, 931, 933, 956, 958, 1123, 1152, 1404
GluI	GCNGC	9 cut(s)	309, 312, 544, 547, 550, 875, 900, 1130, 1252
GsaI	CCCAGC	1 cut(s)	1377
GsuI	CTGGAG	3 cut(s)	547, 1043, 1409
HaeII	RGCGCY	1 cut(s)	1406
HaeIII	GGCC	7 cut(s)	141, 198, 233, 237, 411, 608, 1300
HgaI	GACGC	2 cut(s)	961, 1071
HhaI	GCGC	8 cut(s)	476, 932, 934, 957, 959, 1124, 1153, 1405
Hin1I	GRCGYC	2 cut(s)	762, 1403
Hin1II	CATG	4 cut(s)	49, 112, 120, 1447
Hin6I	GCGC	8 cut(s)	474, 930, 932, 955, 957, 1122, 1151, 1403
HinP1I	GCGC	8 cut(s)	474, 930, 932, 955, 957, 1122, 1151, 1403
HincII	GTYRAC	4 cut(s)	243, 481, 655, 666
HindII	GTYRAC	4 cut(s)	243, 481, 655, 666
HindIII	AAGCTT	1 cut(s)	86
HphI	GGTGA	6 cut(s)	265, 379, 433, 571, 1103, 1180
Hpy166II	GTNNAC	5 cut(s)	243, 481, 655, 666, 1006
Hpy8I	GTNNAC	5 cut(s)	243, 481, 655, 666, 1006
HpyAV	CCTTC	8 cut(s)	15, 165, 364, 517, 538, 620, 937, 1141
HpyCH4III	ACNGT	6 cut(s)	31, 397, 1157, 1232, 1340, 1381
HpyCH4IV	ACGT	8 cut(s)	354, 651, 762, 989, 1076, 1095, 1143, 1452
HpyCH4V	TGCA	4 cut(s)	279, 383, 1235, 1447
HpyF10VI	GCNNNNNNNGC	4 cut(s)	408, 549, 555, 619
HpyF3I	CTNAG	5 cut(s)	150, 318, 603, 885, 1112
HpySE526I	ACGT	8 cut(s)	354, 651, 762, 989, 1076, 1095, 1143, 1452
Hsp92I	GRCGYC	2 cut(s)	762, 1403
Hsp92II	CATG	4 cut(s)	49, 112, 120, 1447
HspAI	GCGC	8 cut(s)	474, 930, 932, 955, 957, 1122, 1151, 1403
KasI	GGCGCC	1 cut(s)	1402
KroI	GCCGGC	1 cut(s)	233
KroNI	GCCGGC	1 cut(s)	235
KspI	CCGCGG	2 cut(s)	547, 1205
Kzo9I	GATC	4 cut(s)	906, 1108, 1344, 1384
LmnI	GCTCC	5 cut(s)	1024, 1119, 1126, 1314, 1370
Lsp1109I	GCAGC	4 cut(s)	320, 911, 1141, 1263
LweI	GCATC	1 cut(s)	1244
MaeI	CTAG	4 cut(s)	176, 1034, 1104, 1217
MaeII	ACGT	8 cut(s)	354, 651, 762, 989, 1076, 1095, 1143, 1452
MalI	GATC	4 cut(s)	908, 1110, 1346, 1386
MbiI	CCGCTC	1 cut(s)	1317
MboI	GATC	4 cut(s)	906, 1108, 1344, 1384
MboII	GAAGA	5 cut(s)	3, 136, 511, 670, 1207
MflI	RGATCY	3 cut(s)	906, 1108, 1344
MhlI	GDGCHC	1 cut(s)	450
MluCI	AATT	2 cut(s)	865, 1331
MluI	ACGCGT	1 cut(s)	1080
Mly113I	GGCGCC	1 cut(s)	1403
MlyI	GAGTC	6 cut(s)	278, 343, 371, 581, 983, 1436
MmeI	TCCRAC	1 cut(s)	1404
MroNI	GCCGGC	1 cut(s)	233
MroXI	GAANNNNTTC	3 cut(s)	518, 699, 1331
MslI	CAYNNNNRTG	2 cut(s)	32, 1158
MspA1I	CMGCKG	4 cut(s)	311, 546, 902, 1204
MspR9I	CCNGG	7 cut(s)	289, 428, 437, 583, 584, 961, 1359
MvaI	CCWGG	3 cut(s)	428, 437, 961
MvnI	CGCG	7 cut(s)	546, 932, 955, 957, 1082, 1124, 1204
MwoI	GCNNNNNNNGC	4 cut(s)	408, 549, 555, 619
NaeI	GCCGGC	1 cut(s)	235
NarI	GGCGCC	1 cut(s)	1403
NciI	CCSGG	4 cut(s)	289, 583, 584, 1359
NcoI	CCATGG	1 cut(s)	45
NdeII	GATC	4 cut(s)	906, 1108, 1344, 1384
NgoMIV	GCCGGC	1 cut(s)	233
NlaIII	CATG	4 cut(s)	49, 112, 120, 1447
NlaIV	GGNNCC	6 cut(s)	502, 634, 749, 1026, 1378, 1404
NmeAIII	GCCGAG	3 cut(s)	559, 1284, 1328
NmuCI	GTSAC	8 cut(s)	119, 332, 439, 490, 640, 949, 1013, 1091
OliI	CACNNNNGTG	2 cut(s)	32, 1158
PaqCI	CACCTGC	1 cut(s)	284
PauI	GCGCGC	2 cut(s)	930, 955
PceI	AGGCCT	1 cut(s)	141
PcsI	WCGNNNNNNNCGW	5 cut(s)	474, 771, 807, 983, 1082
PdiI	GCCGGC	1 cut(s)	235
PdmI	GAANNNNTTC	3 cut(s)	518, 699, 1331
PfeI	GAWTC	4 cut(s)	41, 592, 675, 781
Pfl23II	CGTACG	1 cut(s)	1077
PflFI	GACNNNGTC	1 cut(s)	761
PkrI	GCNGC	9 cut(s)	310, 313, 545, 548, 551, 876, 901, 1131, 1253
PleI	GAGTC	6 cut(s)	278, 343, 370, 581, 982, 1435
PluTI	GGCGCC	1 cut(s)	1406
PmaCI	CACGTG	1 cut(s)	355
PmlI	CACGTG	1 cut(s)	355
PpsI	GAGTC	6 cut(s)	278, 343, 370, 581, 982, 1435
Ppu21I	YACGTR	2 cut(s)	355, 1453
Psp124BI	GAGCTC	1 cut(s)	450
Psp6I	CCWGG	3 cut(s)	426, 435, 959
PspCI	CACGTG	1 cut(s)	355
PspEI	GGTNACC	1 cut(s)	439
PspFI	CCCAGC	1 cut(s)	1373
PspGI	CCWGG	3 cut(s)	426, 435, 959
PspLI	CGTACG	1 cut(s)	1077
PspN4I	GGNNCC	6 cut(s)	502, 634, 749, 1026, 1378, 1404
PspPI	GGNCC	3 cut(s)	632, 747, 1377
PsuI	RGATCY	3 cut(s)	906, 1108, 1344
PsyI	GACNNNGTC	1 cut(s)	761
PteI	GCGCGC	2 cut(s)	930, 955
RigI	GGCCGGCC	1 cut(s)	237
RsaI	GTAC	4 cut(s)	269, 1043, 1079, 1274
RsaNI	GTAC	4 cut(s)	268, 1042, 1078, 1273
RseI	CAYNNNNRTG	2 cut(s)	32, 1158
SacI	GAGCTC	1 cut(s)	450
SacII	CCGCGG	2 cut(s)	547, 1205
SatI	GCNGC	9 cut(s)	309, 312, 544, 547, 550, 875, 900, 1130, 1252
Sau3AI	GATC	4 cut(s)	906, 1108, 1344, 1384
Sau96I	GGNCC	3 cut(s)	632, 747, 1377
SchI	GAGTC	6 cut(s)	278, 343, 371, 581, 983, 1436
ScrFI	CCNGG	7 cut(s)	289, 428, 437, 583, 584, 961, 1359
SduI	GDGCHC	1 cut(s)	450
SfaNI	GCATC	1 cut(s)	1244
SfcI	CTRYAG	1 cut(s)	246
SfoI	GGCGCC	1 cut(s)	1404
Sfr303I	CCGCGG	2 cut(s)	547, 1205
SgrBI	CCGCGG	2 cut(s)	547, 1205
SinI	GGWCC	3 cut(s)	632, 747, 1377
SmaI	CCCGGG	1 cut(s)	584
SmiMI	CAYNNNNRTG	2 cut(s)	32, 1158
SmlI	CTYRAG	2 cut(s)	83, 188
SmoI	CTYRAG	2 cut(s)	83, 188
Sse9I	AATT	2 cut(s)	865, 1331
SseBI	AGGCCT	1 cut(s)	141
SspDI	GGCGCC	1 cut(s)	1402
SspMI	CTAG	4 cut(s)	176, 1034, 1104, 1217
SstI	GAGCTC	1 cut(s)	450
StuI	AGGCCT	1 cut(s)	141
StyD4I	CCNGG	7 cut(s)	287, 426, 435, 581, 582, 959, 1357
StyI	CCWWGG	3 cut(s)	45, 228, 636
TaaI	ACNGT	6 cut(s)	31, 397, 1157, 1232, 1340, 1381
TaiI	ACGT	8 cut(s)	357, 654, 765, 992, 1079, 1098, 1146, 1455
TaqI	TCGA	6 cut(s)	590, 777, 849, 977, 1227, 1335
TaqII	GACCGA	1 cut(s)	683
TasI	AATT	2 cut(s)	865, 1331
TatI	WGTACW	1 cut(s)	267
TauI	GCSGC	5 cut(s)	314, 546, 549, 552, 877
TfiI	GAWTC	4 cut(s)	41, 592, 675, 781
TscAI	CASTG	4 cut(s)	34, 499, 889, 1008
TseFI	GTSAC	8 cut(s)	119, 332, 439, 490, 640, 949, 1013, 1091
TseI	GCWGC	4 cut(s)	308, 899, 1129, 1251
Tsp45I	GTSAC	8 cut(s)	119, 332, 439, 490, 640, 949, 1013, 1091
TspDTI	ATGAA	2 cut(s)	454, 1432
TspGWI	ACGGA	2 cut(s)	316, 1383
TspMI	CCCGGG	1 cut(s)	582
TspRI	CASTG	4 cut(s)	34, 499, 889, 1008
Tth111I	GACNNNGTC	1 cut(s)	761
VpaK11BI	GGWCC	3 cut(s)	632, 747, 1377
XapI	RAATTY	2 cut(s)	865, 1331
XmaI	CCCGGG	1 cut(s)	582
XmnI	GAANNNNTTC	3 cut(s)	518, 699, 1331
XspI	CTAG	4 cut(s)	176, 1034, 1104, 1217
ZraI	GACGTC	1 cut(s)	763

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.