AT2G44810

Phospholipase A(1) DAD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014224
Physical Location & Seq
Forward (+)
18478374 .. 18480457
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44810.1

Sequence Viewer

Length: 1074 bp
ATGGAGTATCAGGGGCTTCAAAATTGGGACGGTCTTTTAGACCCATTGGACGACAATCTCCGGCGAGAGATTCTCCGGTACGGTCAATTTGTCGAATCGGCTTATCAAGCATTTGATTTCGATCCTTCCTCTCCAACCTACGGGACATGCCGGTTTCCGAGGAGCACGTTGTTAGAGCGATCCGGTTTACCCAACTCCGGTTATCGACTAACGAAGAACCTTCGTGCCACGTCAGGTATTAACTTGCCACGTTGGATTGAGAAAGCGCCAAGCTGGATGGCTACACAATCTAGCTGGATTGGTTACGTGGCAGTTTGCCAGGACAAAGAAGAGATCTCGCGGCTTGGGCGTAGAGACGTCGTCATCTCCTTCCGTGGAACCGCCACGTGTCTCGAGTGGTTAGAGAACCTTCGCGCCACGCTGACTCATCTCCCTAATGGGCCTACTGGAGCAAATCTAAACGGGTCTAACTCTGGGCCCATGGTTGAGAGCGGGTTTTTAAGCTTGTATACTTCAGGTGTTCACAGTTTGAGAGACATGGTAAGAGAAGAGATCGCAAGGCTACTCCAATCTTACGGCGACGAGCCGTTAAGTGTAACGATAACCGGTCACAGCCTCGGCGCTGCGATCGCGACACTAGCAGCTTACGATATCAAAACGACGTTTAAACGTGCGCCTATGGTTACCGTAATATCTTTCGGAGGTCCACGTGTCGGAAACAGATGCTTTCGGAAACTCCTTGAGAAGCAAGGCACGAAGGTTCTAAGAATCGTGAACTCCGACGACGTCATCACCAAAGTTCCTGGAGTTGTTTTAGAAAACAGAGAGCAAGATAACGTTAAGATGACAGCGTCGATAATGCCGAGCTGGATACAGAGACGCGTGGAGGAGACGCCGTGGGTTTACGCTGAAATCGGTAAGGAGCTTCGGCTGAGTAGCCGTGACTCGCCGCACTTGAGCAGCATCAATGTGGCCACGTGTCATGAGCTGAAAACGTATTTACATTTGGTAGACGGGTTTGTGAGCTCCACGTGTCCATTCAGAGAAACAGCTCGGAGAGTTCTCCATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko00592 ko01100 ko01110 map00564 map00592 map01100 map01110
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

357

Amino Acids

40.06

Weight (kDa)

8.85

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 121 - 270 8.2e-38 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44810 AT2G44810
fragaria_vesca FvH4_7g10210
malus_domestica MD02G1213100.v1.1 MD07G1109300.v1.1
prunus_persica Prupe.2G132000_v2.0.a1 Prupe.2G132100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0347941
rosa_laevigata RLG00000028702
rosa_multiflora Rmu_ssc0000342.1_g000017
rosa_roxburghii Rroxscaffold_4G00307360
rosa_rugosa Rorug01G0190400
rosa_samantha Rh1AG209000 Rh1BG173600 Rh1CG193200 Rh1DG203600
rosa_wichuraiana Rw1G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 360, 789
AccBSI CCGCTC 1 cut(s) 492
AccI GTMKAC 2 cut(s) 509, 1011
AccII CGCG 4 cut(s) 340, 414, 632, 882
AciI CCGC 4 cut(s) 340, 381, 492, 950
AclI AACGTT 1 cut(s) 837
AclWI GGATC 2 cut(s) 116, 174
AcoI YGGCCR 1 cut(s) 972
AcuI CTGAAG 1 cut(s) 498
AcvI CACGTG 4 cut(s) 387, 710, 978, 1032
AcyI GRCGYC 3 cut(s) 357, 786, 893
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 3 cut(s) 140, 197, 713
AflIII ACRYGT 5 cut(s) 386, 709, 880, 977, 1031
AgeI ACCGGT 1 cut(s) 605
AgsI TTSAA 1 cut(s) 20
AjiI CACGTC 1 cut(s) 231
AjnI CCWGG 2 cut(s) 318, 802
AluBI AGCT 9 cut(s) 273, 294, 504, 644, 867, 925, 988, 1026, 1052
AluI AGCT 9 cut(s) 273, 294, 504, 644, 867, 925, 988, 1026, 1052
Alw21I GWGCWC 2 cut(s) 167, 1028
Alw26I GTCTC 5 cut(s) 348, 395, 528, 871, 884
AlwI GGATC 2 cut(s) 116, 174
Ama87I CYCGRG 1 cut(s) 392
AoxI GGCC 3 cut(s) 440, 476, 972
ApaI GGGCCC 1 cut(s) 480
ApeKI GCWGC 3 cut(s) 623, 641, 960
AsiGI ACCGGT 1 cut(s) 605
AsiSI GCGATCGC 1 cut(s) 630
AspLEI GCGC 4 cut(s) 268, 416, 623, 676
AspS9I GGNCC 4 cut(s) 440, 476, 477, 704
AsuHPI GGTGA 1 cut(s) 784
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 704
BaeGI GKGCMC 1 cut(s) 480
BalI TGGCCA 1 cut(s) 974
BanII GRGCYC 2 cut(s) 480, 1028
BbrPI CACGTG 4 cut(s) 387, 710, 978, 1032
Bbv12I GWGCWC 2 cut(s) 167, 1028
BbvI GCAGC 3 cut(s) 610, 653, 972
BccI CCATC 1 cut(s) 271
BceAI ACGGC 4 cut(s) 571, 592, 880, 924
BciT130I CCWGG 2 cut(s) 320, 804
BciVI GTATCC 1 cut(s) 864
BcoDI GTCTC 5 cut(s) 348, 395, 528, 871, 884
BfaI CTAG 2 cut(s) 291, 638
BfoI RGCGCY 2 cut(s) 269, 624
BfuI GTATCC 1 cut(s) 864
BglII AGATCT 1 cut(s) 333
BisI GCNGC 5 cut(s) 341, 624, 642, 950, 961
BlsI GCNGC 5 cut(s) 342, 625, 643, 951, 962
Bme1390I CCNGG 2 cut(s) 320, 804
Bme18I GGWCC 1 cut(s) 704
BmeT110I CYCGRG 1 cut(s) 392
BmgBI CACGTC 1 cut(s) 231
BmgT120I GGNCC 4 cut(s) 440, 476, 477, 704
BmiI GGNNCC 2 cut(s) 379, 478
BmrFI CCNGG 2 cut(s) 320, 804
BmsI GCATC 2 cut(s) 713, 972
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 2 cut(s) 468, 825
BpuEI CTTGAG 2 cut(s) 761, 976
BsaAI YACGTR 5 cut(s) 307, 387, 710, 978, 1032
BsaBI GATNNNNATC 1 cut(s) 120
BsaHI GRCGYC 3 cut(s) 357, 786, 893
BsaJI CCNNGG 5 cut(s) 158, 373, 480, 616, 896
BsaWI WCCGGW 4 cut(s) 75, 182, 197, 605
BsaXI ACNNNNNCTCC 2 cut(s) 881, 911
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 197, 713
Bse118I RCCGGY 2 cut(s) 150, 605
Bse1I ACTGG 1 cut(s) 451
Bse8I GATNNNNATC 1 cut(s) 120
BseBI CCWGG 2 cut(s) 320, 804
BseDI CCNNGG 5 cut(s) 158, 373, 480, 616, 896
BseGI GGATG 1 cut(s) 282
BseJI GATNNNNATC 1 cut(s) 120
BseLI CCNNNNNNNGG 3 cut(s) 140, 197, 713
BseMII CTCAG 1 cut(s) 923
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 2 cut(s) 175, 902
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 3 cut(s) 610, 653, 972
Bsh1236I CGCG 4 cut(s) 340, 414, 632, 882
Bsh1285I CGRYCG 1 cut(s) 630
BshFI GGCC 3 cut(s) 442, 478, 974
BshTI ACCGGT 1 cut(s) 605
BsiEI CGRYCG 1 cut(s) 630
BsiHKAI GWGCWC 2 cut(s) 167, 1028
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 6 cut(s) 61, 76, 151, 183, 198, 606
BslFI GGGAC 2 cut(s) 41, 157
BslI CCNNNNNNNGG 3 cut(s) 140, 197, 713
BsmAI GTCTC 5 cut(s) 348, 395, 528, 871, 884
BsmBI CGTCTC 3 cut(s) 348, 871, 884
BsmFI GGGAC 2 cut(s) 41, 157
BsnI GGCC 3 cut(s) 442, 478, 974
BsoBI CYCGRG 1 cut(s) 392
Bsp120I GGGCCC 1 cut(s) 476
Bsp1286I GDGCHC 3 cut(s) 167, 480, 1028
Bsp143I GATC 5 cut(s) 121, 179, 333, 552, 627
Bsp19I CCATGG 1 cut(s) 480
Bsp68I TCGCGA 1 cut(s) 632
BspACI CCGC 4 cut(s) 340, 381, 492, 950
BspANI GGCC 3 cut(s) 442, 478, 974
BspCNI CTCAG 1 cut(s) 924
BspFNI CGCG 4 cut(s) 340, 414, 632, 882
BspHI TCATGA 1 cut(s) 982
BspLI GGNNCC 2 cut(s) 379, 478
BspPI GGATC 2 cut(s) 116, 174
BsrBI CCGCTC 1 cut(s) 492
BsrFI RCCGGY 2 cut(s) 150, 605
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 2 cut(s) 150, 605
BssECI CCNNGG 5 cut(s) 158, 373, 480, 616, 896
BssMI GATC 5 cut(s) 121, 179, 333, 552, 627
BssNAI GTATAC 1 cut(s) 510
BssNI GRCGYC 3 cut(s) 357, 786, 893
BssT1I CCWWGG 1 cut(s) 480
Bst1107I GTATAC 1 cut(s) 510
Bst2UI CCWGG 2 cut(s) 320, 804
Bst4CI ACNGT 4 cut(s) 32, 83, 527, 688
Bst6I CTCTTC 2 cut(s) 324, 543
BstACI GRCGYC 3 cut(s) 357, 786, 893
BstBAI YACGTR 5 cut(s) 307, 387, 710, 978, 1032
BstDEI CTNAG 2 cut(s) 764, 932
BstDSI CCRYGG 3 cut(s) 373, 480, 896
BstEII GGTNACC 1 cut(s) 682
BstF5I GGATG 1 cut(s) 282
BstFNI CGCG 4 cut(s) 340, 414, 632, 882
BstH2I RGCGCY 2 cut(s) 269, 624
BstHHI GCGC 4 cut(s) 268, 416, 623, 676
BstKTI GATC 5 cut(s) 124, 182, 336, 555, 630
BstMAI GTCTC 5 cut(s) 348, 395, 528, 871, 884
BstMBI GATC 5 cut(s) 121, 179, 333, 552, 627
BstMCI CGRYCG 1 cut(s) 630
BstMWI GCNNNNNNNGC 4 cut(s) 107, 346, 629, 638
BstNI CCWGG 2 cut(s) 320, 804
BstNSI RCATGY 1 cut(s) 150
BstPI GGTNACC 1 cut(s) 682
BstSCI CCNGG 2 cut(s) 318, 802
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 4 cut(s) 340, 414, 632, 882
BstV1I GCAGC 3 cut(s) 610, 653, 972
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BstZ17I GTATAC 1 cut(s) 510
BsuI GTATCC 1 cut(s) 864
BsuRI GGCC 3 cut(s) 442, 478, 974
BtgI CCRYGG 3 cut(s) 373, 480, 896
BtrI CACGTC 1 cut(s) 231
BtsCI GGATG 1 cut(s) 282
BtuMI TCGCGA 1 cut(s) 632
CciI TCATGA 1 cut(s) 982
CfoI GCGC 4 cut(s) 268, 416, 623, 676
Cfr10I RCCGGY 2 cut(s) 150, 605
Cfr13I GGNCC 4 cut(s) 440, 476, 477, 704
CseI GACGC 3 cut(s) 840, 888, 901
Csp6I GTAC 1 cut(s) 79
CspAI ACCGGT 1 cut(s) 605
CviAII CATG 4 cut(s) 147, 481, 538, 983
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 2 cut(s) 764, 932
DpnI GATC 5 cut(s) 123, 181, 335, 554, 629
DpnII GATC 5 cut(s) 121, 179, 333, 552, 627
DraI TTTAAA 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 972
Eam1104I CTCTTC 2 cut(s) 324, 543
EarI CTCTTC 2 cut(s) 324, 543
Ecl136II GAGCTC 1 cut(s) 1026
Eco130I CCWWGG 1 cut(s) 480
Eco24I GRGCYC 2 cut(s) 480, 1028
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 704
Eco53kI GAGCTC 1 cut(s) 1026
Eco57I CTGAAG 1 cut(s) 498
Eco72I CACGTG 4 cut(s) 387, 710, 978, 1032
Eco88I CYCGRG 1 cut(s) 392
Eco91I GGTNACC 1 cut(s) 682
EcoICRI GAGCTC 1 cut(s) 1026
EcoO65I GGTNACC 1 cut(s) 682
EcoRII CCWGG 2 cut(s) 318, 802
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 480
EcoT38I GRGCYC 2 cut(s) 480, 1028
ErhI CCWWGG 1 cut(s) 480
Esp3I CGTCTC 3 cut(s) 348, 871, 884
FaeI CATG 4 cut(s) 150, 484, 541, 986
FaiI YATR 7 cut(s) 148, 482, 510, 539, 680, 984, 1068
FaqI GGGAC 2 cut(s) 41, 157
FatI CATG 4 cut(s) 146, 480, 537, 982
FauI CCCGC 1 cut(s) 485
FblI GTMKAC 2 cut(s) 509, 1011
Fnu4HI GCNGC 5 cut(s) 341, 624, 642, 950, 961
FokI GGATG 1 cut(s) 289
FriOI GRGCYC 2 cut(s) 480, 1028
Fsp4HI GCNGC 5 cut(s) 341, 624, 642, 950, 961
FspBI CTAG 2 cut(s) 291, 638
GlaI GCGC 4 cut(s) 267, 415, 622, 675
GluI GCNGC 5 cut(s) 341, 624, 642, 950, 961
GsuI CTGGAG 2 cut(s) 468, 825
HaeII RGCGCY 2 cut(s) 269, 624
HaeIII GGCC 3 cut(s) 442, 478, 974
HapII CCGG 6 cut(s) 61, 76, 151, 183, 198, 606
HgaI GACGC 3 cut(s) 840, 888, 901
HhaI GCGC 4 cut(s) 268, 416, 623, 676
Hin1I GRCGYC 3 cut(s) 357, 786, 893
Hin1II CATG 4 cut(s) 150, 484, 541, 986
Hin6I GCGC 4 cut(s) 266, 414, 621, 674
HinP1I GCGC 4 cut(s) 266, 414, 621, 674
HindIII AAGCTT 1 cut(s) 502
HinfI GANTC 5 cut(s) 70, 95, 424, 768, 944
HpaII CCGG 6 cut(s) 61, 76, 151, 183, 198, 606
HphI GGTGA 1 cut(s) 784
Hpy166II GTNNAC 7 cut(s) 188, 510, 523, 707, 775, 904, 1012
Hpy188I TCNGA 7 cut(s) 159, 701, 716, 732, 781, 1043, 1056
Hpy188III TCNNGA 4 cut(s) 392, 631, 772, 983
Hpy8I GTNNAC 7 cut(s) 188, 510, 523, 707, 775, 904, 1012
Hpy99I CGWCG 6 cut(s) 362, 584, 664, 785, 788, 856
HpyAV CCTTC 5 cut(s) 135, 230, 379, 419, 751
HpyCH4III ACNGT 4 cut(s) 32, 83, 527, 688
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 346, 629, 638
HpyF3I CTNAG 2 cut(s) 764, 932
Hsp92I GRCGYC 3 cut(s) 357, 786, 893
Hsp92II CATG 4 cut(s) 150, 484, 541, 986
HspAI GCGC 4 cut(s) 266, 414, 621, 674
Kzo9I GATC 5 cut(s) 121, 179, 333, 552, 627
LmnI GCTCC 4 cut(s) 162, 449, 922, 1031
Lsp1109I GCAGC 3 cut(s) 610, 653, 972
LweI GCATC 2 cut(s) 713, 972
MaeI CTAG 2 cut(s) 291, 638
MaeIII GTNAC 5 cut(s) 302, 595, 608, 682, 941
MalI GATC 5 cut(s) 123, 181, 335, 554, 629
MbiI CCGCTC 1 cut(s) 492
MboI GATC 5 cut(s) 121, 179, 333, 552, 627
MboII GAAGA 3 cut(s) 226, 341, 560
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 3 cut(s) 167, 480, 1028
MlsI TGGCCA 1 cut(s) 974
MluCI AATT 2 cut(s) 22, 86
MluI ACGCGT 1 cut(s) 880
MluNI TGGCCA 1 cut(s) 974
MlyI GAGTC 2 cut(s) 418, 938
MmeI TCCRAC 4 cut(s) 158, 233, 694, 804
MnlI CCTC 5 cut(s) 139, 153, 626, 695, 880
Mox20I TGGCCA 1 cut(s) 974
MscI TGGCCA 1 cut(s) 974
MseI TTAA 5 cut(s) 240, 500, 590, 666, 840
MslI CAYNNNNRTG 1 cut(s) 968
Msp20I TGGCCA 1 cut(s) 974
MspI CCGG 6 cut(s) 61, 76, 151, 183, 198, 606
MspR9I CCNGG 2 cut(s) 320, 804
MssI GTTTAAAC 1 cut(s) 667
MvaI CCWGG 2 cut(s) 320, 804
MvnI CGCG 4 cut(s) 340, 414, 632, 882
MwoI GCNNNNNNNGC 4 cut(s) 107, 346, 629, 638
NcoI CCATGG 1 cut(s) 480
NdeII GATC 5 cut(s) 121, 179, 333, 552, 627
NlaIII CATG 4 cut(s) 150, 484, 541, 986
NlaIV GGNNCC 2 cut(s) 379, 478
NmeAIII GCCGAG 2 cut(s) 597, 888
NmuCI GTSAC 2 cut(s) 608, 941
NruI TCGCGA 1 cut(s) 632
NspI RCATGY 1 cut(s) 150
PaeR7I CTCGAG 1 cut(s) 392
PagI TCATGA 1 cut(s) 982
PcsI WCGNNNNNNNCGW 2 cut(s) 777, 860
PfeI GAWTC 3 cut(s) 70, 95, 768
PflFI GACNNNGTC 3 cut(s) 359, 785, 850
PfoI TCCNGGA 1 cut(s) 802
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 5 cut(s) 342, 625, 643, 951, 962
Ple19I CGATCG 1 cut(s) 630
PleI GAGTC 2 cut(s) 418, 938
PmaCI CACGTG 4 cut(s) 387, 710, 978, 1032
PmeI GTTTAAAC 1 cut(s) 667
PmlI CACGTG 4 cut(s) 387, 710, 978, 1032
PpsI GAGTC 2 cut(s) 418, 938
Ppu21I YACGTR 5 cut(s) 307, 387, 710, 978, 1032
Psp124BI GAGCTC 1 cut(s) 1028
Psp1406I AACGTT 1 cut(s) 837
Psp6I CCWGG 2 cut(s) 318, 802
PspCI CACGTG 4 cut(s) 387, 710, 978, 1032
PspEI GGTNACC 1 cut(s) 682
PspGI CCWGG 2 cut(s) 318, 802
PspN4I GGNNCC 2 cut(s) 379, 478
PspOMI GGGCCC 1 cut(s) 476
PspPI GGNCC 4 cut(s) 440, 476, 477, 704
PsuI RGATCY 1 cut(s) 333
PsyI GACNNNGTC 3 cut(s) 359, 785, 850
PvuI CGATCG 1 cut(s) 630
RgaI GCGATCGC 1 cut(s) 630
RruI TCGCGA 1 cut(s) 632
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
RseI CAYNNNNRTG 1 cut(s) 968
SacI GAGCTC 1 cut(s) 1028
SaqAI TTAA 5 cut(s) 240, 500, 590, 666, 840
SatI GCNGC 5 cut(s) 341, 624, 642, 950, 961
Sau3AI GATC 5 cut(s) 121, 179, 333, 552, 627
Sau96I GGNCC 4 cut(s) 440, 476, 477, 704
SchI GAGTC 2 cut(s) 418, 938
ScrFI CCNGG 2 cut(s) 320, 804
SduI GDGCHC 3 cut(s) 167, 480, 1028
SfaAI GCGATCGC 1 cut(s) 630
SfaNI GCATC 2 cut(s) 713, 972
Sfr274I CTCGAG 1 cut(s) 392
SgfI GCGATCGC 1 cut(s) 630
SinI GGWCC 1 cut(s) 704
SlaI CTCGAG 1 cut(s) 392
SmiMI CAYNNNNRTG 1 cut(s) 968
SmlI CTYRAG 3 cut(s) 392, 740, 955
SmoI CTYRAG 3 cut(s) 392, 740, 955
Sse9I AATT 2 cut(s) 22, 86
SsiI CCGC 4 cut(s) 340, 381, 492, 950
SspMI CTAG 2 cut(s) 291, 638
SstI GAGCTC 1 cut(s) 1028
StyD4I CCNGG 2 cut(s) 318, 802
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 4 cut(s) 32, 83, 527, 688
TaqI TCGA 5 cut(s) 93, 120, 205, 393, 854
TasI AATT 2 cut(s) 22, 86
TauI GCSGC 2 cut(s) 343, 952
TfiI GAWTC 3 cut(s) 70, 95, 768
Tru1I TTAA 5 cut(s) 240, 500, 590, 666, 840
Tru9I TTAA 5 cut(s) 240, 500, 590, 666, 840
TseFI GTSAC 2 cut(s) 608, 941
TseI GCWGC 3 cut(s) 623, 641, 960
Tsp45I GTSAC 2 cut(s) 608, 941
TspGWI ACGGA 1 cut(s) 362
Tth111I GACNNNGTC 3 cut(s) 359, 785, 850
VpaK11BI GGWCC 1 cut(s) 704
XceI RCATGY 1 cut(s) 150
XhoI CTCGAG 1 cut(s) 392
XmiI GTMKAC 2 cut(s) 509, 1011
XspI CTAG 2 cut(s) 291, 638
ZraI GACGTC 2 cut(s) 358, 787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.